Exogenous abscisic acid improves grain filling capacity under heat stress by enhancing antioxidative defense capability in rice.

BACKGROUND: Heat stress is a major restrictive factor that causes yield loss in rice. We previously reported the priming effect of abscisic acid (ABA) on rice for enhanced thermotolerance at the germination, seedling and heading stages. In the present study, we aimed to understand the priming effect and mechanism of ABA on grain filling capacity in rice under heat stress. RESULTS: Rice plants were pretreated with distilled water, 50 µM ABA and 10 µM fluridone by leaf spraying at 8 d or 15 d after initial heading (AIH) stage and then were subjected to heat stress conditions of 38 °C day/30 °C night for 7 days, respectively. Exogenous ABA pretreatment significantly super-activated the ABA signaling pathway and improved the SOD, POD, CAT and APX enzyme activity levels, as well as upregulated the ROS-scavenging genes; and decreased the heat stress-induced ROS content (O2- and H2O2) by 15.0-25.5% in rice grain under heat stress. ABA pretreatment also increased starch synthetase activities in rice grain under heat stress. Furthermore, ABA pretreatment significantly improved yield component indices and grain yield by 14.4-16.5% under heat stress. ABA pretreatment improved the milling quality and the quality of appearance and decreased the incidence of chalky kernels and chalkiness in rice grain and improved the rice grain cooking quality by improving starch content and gel consistence and decreasing the amylose percentage under heat stress. The application of paraquat caused overaccumulation of ROS, decreased starch synthetase activities and ultimately decreased starch content and grain yield. Exogenous antioxidants decreased ROS overaccumulation and increased starch content and grain yield under heat stress. CONCLUSION: Taken together, these results suggest that exogenous ABA has a potential priming effect for enhancing rice grain filling capacity under heat stress at grain filling stage mainly by inhibiting ROS overaccumulation and improving starch synthetase activities in rice grain.

Effects of landscape conservation on the ecohydrological and water quality functions and services and their driving factors.

Since the implementation of landscape conservation of the green heart area in the Changsha-Zhuzhou-Xiangtan Metropolitan Region, the landscape structure and pattern have changed significantly. The ecosystem service functions in the area have been improved, but the status of ecohydrological and water quality and service functions (EHWQSFs) is still unclear. To clarify the status of EHWQSFs and their driving factors influenced by landscape conservation, this study analysed landscape changes using remote sensing image data from 1998, 2008, and 2018 and the changes and their spatial characteristics using the Soil and Water Assessment Tool (SWAT) and spatial analysis methods. The results showed that the dominant land types in the area were forestland and cropland from 1998 to 2018; the area of forestland and construction land expanded and that of cropland decreased year by year; the annual average surface runoff volume rose, and the annual average actual evapotranspiration and soil water content fell from 1998 to 2008 and rose from 2008 to 2018; and all pollutant indicators decreased significantly after 2008. The areas with higher surface runoff were mainly concentrated in the central and southern regions, those with higher evapotranspiration were in the northwestern and southwestern regions, those with higher soil water content were in the northern region, and those with higher sediment and nitrogen and phosphorus pollutant contents were in the central and southeastern regions. The results showed that land use, land cover and meteorological factors were the most significant drivers on EHWQSFs and illustrated that EHWQSFs in the area decreased after 1998. There was a significant improvement after 2008 and the area currently has a good status. This study not only provides insights into land use, land cover and meteorological factors that have significant impacts on EHWQSFs but also highlights that the landscape conservation of the area can improve ecosystem service functions.