RESUMO
A novel AluI-polymorphism in the fourth intron of chicken growth hormone gene was shown. It was detected the cytosine to thymine transition in the restriction site for AluI. Primers, that flanking the 460 bp fragment of the fourth intron, containing a polymorphic restriction site for AluI, was designed. The nucleotide sequence fragments amplified polymorphic variants was determined. Using designed primers was analyzed the genetic structure of populations of White Plymouth Rock, Poltava Clay, Rhode Island Red and Borkovskaya Barvistaya chicken breeds. It was found that growth hormone gene (by AluI-polymorphism in the fourthintron) was polymorphic in all experimental populations. Frequencies of alleles C and T in chicken population of White Plymouth Rock breed were 0,14 and 0,86; Rhode Island Red 0,3 and 0,7; Poltava Clay 0,04 and 0,96; Borkovskaya Barvistaya 0,08 and 0,92 respectively. The tendency to increase egg production and egg weight of chicken with C/C genotype, as well as meat quality (live weight, carcass weight, weight of pectoral muscles) of chickens with genotype T/T of Rhode Island Red chicken breed was shown.
Assuntos
Galinhas/genética , Desoxirribonucleases de Sítio Específico do Tipo II/química , Hormônio do Crescimento/genética , Íntrons , Polimorfismo de Fragmento de Restrição , Característica Quantitativa Herdável , Alelos , Animais , Sequência de Bases , Cruzamento , Galinhas/anatomia & histologia , Galinhas/classificação , Ovos , Feminino , Expressão Gênica , Frequência do Gene , Genótipo , Masculino , CarneRESUMO
The formation of different types of the artifacts in the amplification reaction with using different classes of the molecular-genetic markers (Indel and SSR) was studied. It was shown that DNA heteroduplexes formed during amplification of heterozygous samples, as fragments of target genes and microsatellite loci. During the electrophoresis in native polyacrylamide gel of the amplification products of homozygous samples for microsatellite loci it was revealed specific additional fragments that do not belong to the class of heteroduplex DNA. It was supposed, that the additional fragments belong to a special type of homoduplex DNA non-linear homoduplexes. The analysis revealed that the formation of a non-linear homoduplex DNA takes place on the 2025 cycle of the PCR, both at the amplification of the individual samples, and individual DNA fragments that was cut out from the gel.
