Assuntos
Diabetes Mellitus Experimental/cirurgia , Transplante das Ilhotas Pancreáticas/fisiologia , Animais , Glicemia/metabolismo , Diabetes Mellitus Experimental/sangue , Teste de Tolerância a Glucose , Insulina/sangue , Masculino , Mesocolo , Monitorização Fisiológica/métodos , Ratos , Ratos Endogâmicos , Transplante Heterólogo/métodos , Transplante IsogênicoRESUMO
The iodo derivative of histamine labelled with 125I has been used for many years to prepare tracers used in RIA systems. The aim of this study was to evaluate radioiodinated histamine as a potential isotope carrier for in vivo applications. The biological behaviour of radioiodinated histamine has been investigated in rodents. The observed absence of any specific iodohistamine uptake by a critical organ or tissue promises a very quick distribution of the iodohistamine in soft tissues, and a rapid rate of whole-body clearance via the urinary tract (e.g. over 50% of the injected dose (ID) during the first hour after administration). In spite of moderately low in vitro stability of iodohistamine in serum, biodistribution studies in rodents have not shown any significant release of iodine from the parent molecule in the whole animal. Low uptake was observed in the thyroid (e.g. 0.22 and 0.11% ID at 1 and 2 h after administration to rats), and not more than 3% of injected activity was detected in the stomach in all of the biodistribution experiments. Moreover, our results refute any possibility of competition between histamine and iodohistamine for receptor binding sites, and suggest that radioactive mono-iodohistamine may be used successfully to develop some new radiolabelled bioactive molecules with potential application in vivo.
Assuntos
Histamina/farmacocinética , Radioisótopos do Iodo/farmacocinética , Compostos Radiofarmacêuticos/farmacocinética , Animais , Estabilidade de Medicamentos , Histamina/química , Histamina/urina , Radioisótopos do Iodo/química , Radioisótopos do Iodo/urina , Masculino , Camundongos , Compostos Radiofarmacêuticos/urina , Ratos , Ratos Wistar , Distribuição TecidualRESUMO
BACKGROUND: The unique mechanism of dithizone action in the interior of the viable pancreatic islet suggests the possible development of a specific radiopharmaceutical that may have a potential clinical application in the diagnosis of the pancreatic organ allografts or islets rejection. The radiodiagnostic properties of the newly developed radioactive analogue of dithizone, i.e. Dithizone-[(131)I]-Histamine conjugate have been evaluated in the present study. METHODS: The four islet cells transplantation models were chosen for this purpose. The most important feature of the Dithizone-[(131)I]-Histamine conjugate is its possessed ability of zinc chelation. As was presented in the recent study, the conjugate stains pink-reddish the isolated pancreatic islets in vitro. Among the studied transplantation models, only the islets grafting under testis capsule enabled determination of the pancreatic islets in rats by radioactive Dithizone-[(131)I]-Histamine conjugate. The level of the radioactivity in the recipient testis (right) was almost two times higher compared to the controls (0.24 vs. 0.13% ID/g, respectively). CONCLUSIONS: These preliminary data demonstrate the ability of the developed radioactive analogue of dithizone for in vivo identification of transplanted pancreatic islets, and suggests a potential clinical application of the radiodithizone in the diagnosis of the pancreatic islet rejection.
RESUMO
Synthesis, characteristics and radioiodination of the new carboxylic derivatives of dithizone are described in this paper. We have applied the carboxy dithizones for preparation of radioactive compounds by coupling with [131I]-histamine. Preliminary biological studies of the new radiodithizone were done in rats after two different application routs: peripheral i.v. injection and direct injection to splenic artery. Biodistribution of the carboxy dithizone-[131I]-histamine conjugate (i.v. injection) was quite different than that for free [131I]-histamine. However, uptake of activity in pancreas was low (0.81% g-1 of tissue). Direct application of the conjugate to splenic artery resulted in high activity retention in pancreas after 30 and 45 min post injection (respectively 8.8 and 12.4% g-1 of tissue) indicating potential usefulness of the new radiodithizone for in vivo monitoring of pancreas.
Assuntos
Ácidos Carboxílicos/farmacocinética , Ditizona/análogos & derivados , Ditizona/síntese química , Ditizona/farmacocinética , Radioisótopos do Iodo , Animais , Histamina/farmacocinética , Radioisótopos do Iodo/farmacocinética , Ratos , Iodeto de Sódio , Fatores de Tempo , Distribuição TecidualAssuntos
Diabetes Mellitus Experimental/cirurgia , Rejeição de Enxerto/imunologia , Transplante das Ilhotas Pancreáticas/imunologia , Isoantígenos/imunologia , Timo/imunologia , Imunologia de Transplantes , Animais , Diabetes Mellitus Experimental/imunologia , Tolerância Imunológica , Ratos , Ratos Endogâmicos Lew , Ratos Wistar , Transplante HomólogoRESUMO
Transplantation of whole pancreas or pancreatic islets remains a promising approach to treatment of diabetes mellitus. Since there is no efficient method presently known for in vivo detection of pancreatic islet rejection, we have utilized dithizone [DTZ] to monitor the survival of transplanted islet allografts following the induction of tolerance by a new strategy of deliberate introduction of donor antigens into the adult thymus. In this study, we examined the morphology of islet allografts in vivo and in vitro following pretreatment with intrathymic (IT) inoculation of 2 mg soluble Ag obtained from 3M KCl extracts of resting T-cells with or without ALS immunosuppression in the WF-to-Lewis combination. Fresh isolated rat islets stained pink 3-5 minutes following exposure to medium containing 0.12 mM DTZ solution in DMSO. Intravenous (i.v.) injection of DTZ solution into unmodified recipients of islet allografts that had rejected their grafts showed massive degranulation of islets which did not stain pink with DTZ. This was confirmed by microscopic finding of fibrosis and lymphocytic infiltration. In contrast, i.v. injection of DTZ solution into long-term recipients of islet allografts at 50, 100, and 150 days after transplantation showed viable islet cells which stained crimson red with DTZ and the findings were confirmed with microscopic sections. This study demonstrates that DTZ is an effective means of in vivo and in vitro identification of transplanted pancreatic islets and suggests that this strategy may have potential clinical application in the diagnosis of the pancreatic islet rejection.
Assuntos
Ditizona , Indicadores e Reagentes , Transplante das Ilhotas Pancreáticas/imunologia , Isoantígenos/administração & dosagem , Imunologia de Transplantes , Animais , Sobrevivência de Enxerto , Isoantígenos/uso terapêutico , Ratos , Ratos Endogâmicos Lew , Ratos Endogâmicos WF , TimoAssuntos
Glicemia/metabolismo , Diabetes Mellitus Experimental/cirurgia , Transplante das Ilhotas Pancreáticas/métodos , Animais , Diabetes Mellitus Experimental/sangue , Rejeição de Enxerto/patologia , Transplante das Ilhotas Pancreáticas/patologia , Transplante das Ilhotas Pancreáticas/fisiologia , Neovascularização Fisiológica , Ratos , Ratos Endogâmicos Lew , Transplante HomólogoAssuntos
Corantes , Ditizona , Transplante das Ilhotas Pancreáticas/patologia , Coloração e Rotulagem/métodos , Animais , Corantes/administração & dosagem , Corantes/toxicidade , Ditizona/administração & dosagem , Ditizona/toxicidade , Humanos , Técnicas In Vitro , Ilhotas Pancreáticas/efeitos dos fármacos , Ilhotas Pancreáticas/patologia , Ratos , Ratos Endogâmicos Lew , SegurançaRESUMO
Dithizone (DTZ) which selectively stains islets of Langerhans in vitro has a diagnostic potential in the in vivo identification of viable transplanted pancreatic islets. In the present study, we compared the use of DTZ, and a synthetic iodo-derivative of DTZ (I-DTZ) as a potential radioactive marker for identification of viable transplanted pancreatic islet cells. Fresh islets isolated from Lewis rat donors were transplanted beneath the kidney capsule into each diabetic syngeneic (Lewis) recipient rat. DTZ(I-DTZ) solution was injected intravenously (i.v.) at a dose 10 mg/kg body weight (b.w.) for macro and microscopic identification of surviving transplanted islets. The recipients were nephrectomized to confirm that the normoglycemic state was maintained by the islet grafts. In addition, we administered i.v. at the same time concentrations of DTZ (I-DTZ) to test for toxicity. The results show that following i.v. or intraductal (i.d.) injection of low concentrations does not damage pancreatic islet function as determined by insulin secretion or glucose levels. Angiogenesis and microvascularization were observed for 10-12 days after transplantation, and the histologic and biochemical studies showed no pathological changes in injected rats in organ examined. The human pancreatic islets harvested from multiorgan donors stain red ex vivo in the same way as DTZ or I-DTZ administered i.v. in rats. The results indicate that DTZ has a diagnostic potential in monitoring the survival of transplanted pancreatic islets and possibly in the early diagnosis by radioisotopic detection of pancreatic endocrine diseases.
Assuntos
Corantes , Ditizona/farmacologia , Sobrevivência de Enxerto , Ilhotas Pancreáticas/efeitos dos fármacos , Animais , Química Farmacêutica , Dimetil Sulfóxido , Ditizona/toxicidade , Etanol , Humanos , Insulina/metabolismo , Ilhotas Pancreáticas/metabolismo , Transplante das Ilhotas Pancreáticas , Neoplasias Pancreáticas/diagnóstico , Ratos , SolventesAssuntos
Ditizona/análogos & derivados , Transplante das Ilhotas Pancreáticas/fisiologia , Ilhotas Pancreáticas/citologia , Alanina Transaminase/sangue , Amilases/sangue , Animais , Aspartato Aminotransferases/sangue , Bilirrubina/sangue , Glicemia/metabolismo , Sobrevivência Celular , Creatinina/sangue , Técnicas de Cultura/métodos , Glucose/farmacologia , Insulina/metabolismo , Secreção de Insulina , Ilhotas Pancreáticas/efeitos dos fármacos , Ilhotas Pancreáticas/metabolismo , Transplante das Ilhotas Pancreáticas/patologia , Ratos , Ratos Endogâmicos , Coloração e RotulagemRESUMO
The mono and diiodo-derivatives of dithizone labelled with radioactive 131I isotope were obtained. Those compounds can be used for diagnostic of pancreas and other organs to monitor pathological states.
Assuntos
Ditizona/análogos & derivados , Compostos de Iodo/síntese química , Ilhotas Pancreáticas/metabolismo , Pancreatopatias/diagnóstico , Animais , Ditizona/farmacocinética , Compostos de Iodo/farmacocinética , Radioisótopos do Iodo , Masculino , Ratos , Ratos Wistar , Distribuição TecidualRESUMO
A simple, one-step method of preparation of human albumin microspheres by thermal denaturation of protein aerosol in a gas medium is described. These microspheres were easily labelled with technetium-99m and iodine-131, and were characterized by short biological clearance and high lung uptake.
Assuntos
Albumina Sérica , Animais , Meia-Vida , Temperatura Alta , Humanos , Radioisótopos do Iodo/metabolismo , Fígado/metabolismo , Pulmão/metabolismo , Métodos , Camundongos , Microesferas , Desnaturação Proteica , Albumina Sérica/isolamento & purificação , Albumina Sérica/metabolismo , Baço/metabolismo , Tecnécio/metabolismo , Agregado de Albumina Marcado com Tecnécio Tc 99mAssuntos
Índio , Fígado/diagnóstico por imagem , Radioisótopos , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Hepatopatias/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Ácido Fítico , CintilografiaAssuntos
Nefropatias/diagnóstico por imagem , Cintilografia/métodos , Adolescente , Adulto , Idoso , Animais , Ácido Ascórbico , Carga Corporal (Radioterapia) , Combinação de Medicamentos , Estudos de Avaliação como Assunto , Feminino , Gluconatos , Humanos , Radioisótopos do Iodo , Masculino , Radioisótopos de Mercúrio , Pessoa de Meia-Idade , Ácido Pentético , Ratos , Tecnécio , Distribuição TecidualRESUMO
Mice injected with 131I-human albumin microspheres equivalent to 10-20 or 200 human doses were sequentially killed over a 12-day period. About 90% of the microspheres initially lodged in the precapillary arterioles and capillaries of the lungs. Their pulmonary clearance was essentially complete after 3 days. Occlusion of the vessels always led to focal hyperemia of the surrounding tissue and to slight hemorrhage into the alveoli. This was followed, though less frequently, by perivascular nodular inflammtion. Hemorrhagic infarcts were quite uncommon and occurred only after the massive doses. Some emboli underwent organization, but most were resolved. Circulatory disturbances and perivascular inflammation receded in about 1 week and seldom led to obliteration of the involved vessels. Hemorrhagic infarcts were converted into minute scars. Twelve days after injection of microspheres in massive doses, the only findings were post-inarct scars and obliterated vessels, which were sparse and difficult to detect. The lower doses of microspheres did not leave any detectable residues.