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2.
Neuroscientist ; 20(5): 426-31, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24122821

RESUMO

Neurons are exquisitely specialized for rapid electrical transmission of signals, but some properties of glial cells, which do not communicate with electrical impulses, are well suited for participating in complex cognitive functions requiring broad spatial integration and long-term temporal regulation. Astrocytes, microglia, and oligodendrocytes all have biological properties that could influence learning and cognition. Myelination by oligodendrocytes increases conduction velocity, affecting spike timing and oscillations in neuronal activity. Astrocytes can modulate synaptic transmission and may couple multiple neurons and synapses into functional assemblies. Microglia can remove synapses in an activity-dependent manner altering neural networks. Incorporating glia into a bicellular mechanism of nervous system function may help answer long-standing questions concerning the cellular mechanisms of learning and cognition.


Assuntos
Cognição/fisiologia , Aprendizagem/fisiologia , Neuroglia/fisiologia , Animais , Encéfalo/fisiologia , Humanos , Neurogênese/fisiologia , Percepção Espacial/fisiologia
3.
Microsc Microanal ; 13(2): 108-17, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17367550

RESUMO

The inaccessibility of osteocytes due to their embedment in the calcified bone matrix in vivo has precluded direct demonstration that osteocytes use gap junctions as a means of intercellular communication. In this article, we report successfully isolating primary cultures of osteocytes from chick calvaria, and, using anti-connexin 43 immunocytochemistry, demonstrate gap junction distribution to be comparable to that found in vivo. Next, we demonstrate the functionality of the gap junctions by (1) dye coupling studies that showed the spread of microinjected Lucifer Yellow from osteoblast to osteocyte and between adjacent osteocytes and (2) analysis of fluorescence replacement after photobleaching (FRAP), in which photobleaching of cells loaded with a membrane-permeable dye resulted in rapid recovery of fluorescence into the photobleached osteocyte, within 5 min postbleaching. This FRAP effect did not occur when cells were treated with a gap junction blocker (18alpha-glycyrrhetinic acid), but replacement of fluorescence into the photobleached cell resumed when it was removed. These studies demonstrate that gap junctions are responsible for intercellular communication between adjacent osteocytes and between osteoblasts and osteocytes. This role is consistent with the ability of osteocytes to respond to and transmit signals over long distances while embedded in a calcified matrix.


Assuntos
Comunicação Celular/fisiologia , Junções Comunicantes/fisiologia , Osteoblastos/fisiologia , Osteócitos/fisiologia , Crânio/fisiologia , Animais , Comunicação Celular/efeitos dos fármacos , Embrião de Galinha , Conexina 43/fisiologia , Corantes Fluorescentes/química , Ácido Glicirretínico/análogos & derivados , Ácido Glicirretínico/farmacologia , Isoquinolinas/química , Microscopia Eletrônica de Varredura , Microscopia de Fluorescência , Osteoblastos/citologia , Osteócitos/citologia , Crânio/citologia
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