RESUMO
In this paper we present a wearable device for continuous monitoring of respiration signal and the associated algorithm for signal evaluations. The device took advantages of a proven respiratory inductive plethysmograph (RIP) technology and a wireless body sensor networks (BSN) development platform. The textile RIP sensor was integrated into a suit that could be comfortably worn around thorax or abdomen for monitoring respiration during sleep. A smart signal processing algorithm was implemented for extracting the dynamic respiration rate. The results of in-situ experiments from ten healthy subjects suggested that our system worked as intended. Due to the high reliability and low cost of our system it is believed to meet the future demands on home-based monitoring and diagnosis of sleep disorder-related diseases.
Assuntos
Desenho de Equipamento/métodos , Monitorização Fisiológica/instrumentação , Monitorização Fisiológica/métodos , Pletismografia/métodos , Respiração , Algoritmos , Humanos , Síndromes da Apneia do Sono/diagnóstico , SoftwareRESUMO
This paper proposed a low-complexity medium access control (MAC) protocol tailored for body sensor networks (BSN) applications. The MAC protocol was designated to handle collision avoidance by reducing the numbers of the overhead packets for handshake control within the BSN. We also suggested a novel message recovery mechanism for getting back the lost physiological information. The adaptive synchronization scheme we have implemented exploited the features of multiple data-rate and adjustable precision design to support differentiated healthcare applications. The MAC protocol was fully implemented using our BSN development platform. The experimental results suggested the improved MAC design was compact and energy-efficient.
Assuntos
Redes de Comunicação de Computadores/instrumentação , Monitorização Ambulatorial/instrumentação , Telemetria/instrumentação , Transdutores , Algoritmos , Segurança Computacional/instrumentação , Simulação por Computador , Computadores , Fontes de Energia Elétrica , Desenho de Equipamento , Humanos , Telemedicina/instrumentaçãoRESUMO
OBJECTIVE: To study the benzo (a) pyrene (BaP) induced DNA adduct in rat and establish a method to measure the DNA adduct in blood by high performance liquid chromatography (HPLC). METHODS: The SD rats were treated with 100 mg/kg of BaP-DMSO (cosolvent: 1% sodium carboxymethyl cellulose) once by i.p., and the blood of femoral vein was collected 5 hours later. The blood DNA was extracted by kit and confirmed by agarose gel electrophoresis. After extraction, the DNA adducts were hydrolyzed in 0.1 nmol/L HCl at 90 degrees C for 4 hours. The acid-hydrolysis products (BP-tetrols) of DNA adducts were extracted by ethyl acetate and measured by HPLC, and finally confirmed by HPLC-MS. RESULTS: In chromatogram there were new peaks to occur for rats treated by BaP, with compared to control. By HPLC-MS, one of the new peaks was confirmed to be BP-tetrol. CONCLUSION: The rats ingesting BaP in the way described in this experiment can form DNA adducts, and the adducts in blood can be detected by HPLC.
