Use of masking strategies to avoid the boar taint perception in chorizo: consumers' acceptability.

The production of entire male pigs is an alternative to surgical castration. However, boar taint may be present in the meat of entire male animals. The aim of the present paper was to investigate the acceptability and sensory properties of chorizo, elaborated under three different levels of androstenone [castrated 0-0.3 mg / kg, medium 0.4-0.9 mg / kg and high 1-2.75 mg / kg] and two masking strategies [rosemary, and rosemary plus smoking] using a Check-All-That-Apply questionnaire. The results showed that, of the strategies employed, the rosemary plus smoked chorizo was the most effective masking strategy to reduce the boar taint in chorizos with high androstenone level (3.7% selection rate for animal flavour), while the closest to the ideal product, according to consumers' description, was high androstenone level plus rosemary chorizo.

Rustic Chato Murciano pig breed: Effect of the weight on carcass and meat quality.

Carcass and meat quality parameters of two different weights of pigs from the rustic breed Chato Murciano (Murcia Region, south-eastern Spain) were studied: light weight (LW, 147.98 ±â€¯3.78 kg live weight) and heavy weight (HW, 176.07 ±â€¯4.78 kg live weight). No weight-dependent effects were observed on carcass quality (carcass yield, length, back fat thickness) or on moisture, pH, and the L* and a* coordinates. The HW group had a higher fat content and cholesterol values than the LW group. None of the fatty acids analysed in the respective meats showed differences between groups (P > .05), except C16:0, C18:2 and C18:3. The HW group had higher values for unsaturated and polyunsaturated fatty acids and the n6/n3 index than LW, and lower values for saturated fat and the Saturated/Polyunsaturated fat ratio and a lower Atherogenesis Index. In conclusion, meat and fat quality are influenced by the final live weight of Chato Murciano pigs.

Consumers' sensitivity to androstenone and the evaluation of different cooking methods to mask boar taint.

Boar taint is an unpleasant odour and flavour present in some entire male pigs that is due to the presence of androstenone and skatole. The aim of the study was to assess the sensitivity of 150 consumers to androstenone and to compare the acceptability and liking of meat from castrated and entire pigs, cooked with different cooking methods. Meat samples consisted of loins from castrated (CM) and entire male pigs (EM) with high levels of androstenone cooked by two cooking methods: sous-vide and fried/breaded with garlic and parsley. Consumers evaluated smell and flavour acceptability, and overall liking of CM and EM for each cooking method. The results of the study showed that dislike of androstenone odour increased significantly with sensitivity. The results of acceptability and overall liking were similar in CM and EM for both cooking methods. Therefore, the two cooking methods used in the study may be useful to mask boar taint.

Evaluation of different strategies to mask boar taint in cooked sausage.

The use of smoking and/or spices was evaluated for their ability to mask boar taint in frankfurters manufactured from entire pigs with high levels of androstenone. Five frankfurter types were considered: control, smoked, flavouring+smoked, spicy and spicy+smoked. A trained panel in androstenone perception carried out a sensory profile on the different sausages. The highest scores for androstenone perception (odour, flavour and aftertaste) were found in frankfurters that included no masking strategy which indicated the effectiveness of the evaluated strategies. Regarding masking strategies, the contribution of spices and smoking to sensory perception of frankfurters was detected by the panellists. Smoking was the best strategy to mask androstenone odour, while the use of spices masked androstenone odour to a greater extent than androstenone flavour. Only the combined use of spices and smoking was able to eliminate the perception of androstenone. The application of this strategy in frankfurters could be an alternative in the commercialization of entire pigs.

Development of a convenience and safety chilled sous vide fish dish: Diversification of aquacultural products.

The dynamic expansion of the ready-to-eat seabream sector in its adaptation to new lifestyles has led to the search for new presentation formats in seabream (Sparus aurata). Green sauce (olive oil, wine vinegar, garlic, fresh parsley, black pepper, basil and salt) and 60 ℃ of cooking temperature were chosen by the panellists for the sous vide cooking process. Seabream fillet and sauce were packaged in polypropylene trays, cooked, chilled and stored at 2 ℃. Microbiological (total viable counts,Enterobacteriaceae,lactic acid bacteria, anaerobic psychrotrophic, moulds and yeasts, Salmonella and Listeria monocytogenes), chemical (pH and TBARs) and sensory parameters were determined at 0, 7, 17, 34, 48 and 62 days. In the conditions used, the microbiological counts remained stable, and Salmonella and Listeria monocytogenes were absent. The acidic sauce had a positive effect on the pH of the product, and low TBARs were obtained throughout storage. The processing conditions used in the present study allowed a chilled ready-to-eat seabream product of consistently high quality up to 62 days of storage to be obtained, representing an expansion of the products offered by the aquacultural industry.

Feeding Iberian × Duroc cross pigs with crude glycerine: Effects of diet and gender on carcass and meat quality.

Ninety (45 castrated males and 45 gilts) Iberian × Duroc pigs were used in this study. During the finishing period (95-160 kg body weight, 74 days) animals received conventional feed (control G0) or the same feed plus 5% (G5) or 10% (G10) of crude glycerine to partially replace wheat. In general, neither the diet nor gender affected ultrasound, carcass or meat quality parameters. The G10 had lower values of cooking loss and a* than the G5 and G0 groups. The fatty acids, C10:0, C12:0 and C18:3, in intramuscular fat were lower in both glycerine groups. Polyunsaturated fatty acids and 18:2 decreased and C20:0 increased at the subcutaneous fat of G10 animals. Castrated males produced carcasses with higher backfat thickness and fat content. Castrated males had a higher SFA content in subcutaneous fat than females (P<0.001). In conclusion, up to 10% crude glycerine could be used in the Iberian × Duroc diet.

TBARs distillation method: revision to minimize the interference from yellow pigments in meat products.

The aim was to study the effect of the incubation method and TBA reagent (concentration/solvent) on yellow pigment interference in meat products. Distillates from red sausage, sucrose, malondialdehyde and a mixture of sucrose-malondialdehyde were reacted with four different TBA solutions at five different temperature/time relations. Two TBA solutions were prepared at 20mM using 90% glacial acetic acid or 3.86% perchloric acid. In addition, an 80mM TBA solution was prepared using distilled water adjusted to pH4 and another using 0.8% TBA in distilled water. The temperature/time relations were: (1) 35min in a boiling water bath; (2) 70°C/30min; (3) 40°C/90min; (4) room temperature (r.t.) (24°C) in dark conditions for 20h; and (5) 60min in a boiling water bath. The results showed that aqueous or diluted acid solutions of TBA reagent and the application of 100°C for less than 1h provided the best conditions to minimize the presence of yellow pigments and maximize pink pigment formation in meat products.

Crude glycerine inclusion in Limousin bull diets: animal performance, carcass characteristics and meat quality.

Three hundred and six Limousin young bulls (7±1months of age, initial body weight 273±43kg) were used to evaluate the effect of crude glycerine supplementation on animal performance, carcass characteristics and meat quality. Animals were assigned to three different diets: Control (0% of crude glycerine), G2 and G4 (2 and 4% of crude glycerine, respectively). The diets were administrated ad libitum for 240days (final body weight 644±24kg). Average daily weight gain, average daily feed intake, the gain:feed ratio, ultrasound measures in vivo, carcass characteristics, pH, water holding capacity, drip losses, and cooking losses were not affected (P>0.05) by diets. Diet decreased C16:0 (P<0.01) and C16:1 (P<0.05) contents in meat. The G4 meat showed lower C12:0, C14:0, C17:0, C18:0, C18:1, C18:2, C18:3, c9,t11-c18:2, C20:0 and C20:4 levels (P<0.05) than control. Glycerine increased desirable fatty acid percentages (P<0.05) in intramuscular fat.

Chemical and sensory quality of lamb meat burgers from Manchego Spanish breed.

This study examines the nutritional composition, fatty acid profile and sensory properties of two types of lamb burgers from the Spanish Manchego breed (formula 1 = L: completely from leg lamb meat; formula 2 = LNB with 2/3 leg and 1/3 neck and breast meat). A significant effect of the formulation type was found since Formula 1 had a lower fat percentage (p < 0.001) and higher protein content (p < 0.05). The percentage of total fatty acids and the profile varied for both formulas and consequently their indexes. In addition, the levels of saturated and polyunsaturated fatty acids were slightly lower (p < 0.001) and higher (p < 0.001), respectively, in burgers L than in LNB although both types of burgers raised fatty acid indexes in a healthy threshold. As regarding the sensorial analysis, non-significant differences were described among both formulas. In conclusion, meat quality characteristics were similar for both formulation types being the storage time, the only factor affecting lamb burger stability.

Effect of different gas stunning methods on Manchega suckling lamb meat packed under different modified atmospheres.

Forty-nine Manchega breed male suckling lambs were used in this experiment. The effect of CO(2) concentration and exposure time at stunning [80% CO(2) for 90 s (G1); 90% CO(2) for 90 s (G2); 90% CO(2) for 60 s (G3); 80% CO(2) for 60 s (G4)] plus an electrically stunned control group (G5) was assessed for pH, colour (L(*), a(*), b(*), C(*) and h(*)), water holding capacity (WHC), drip loss (DL), cooking loss (CL) and shear force (SF) in samples packed under two different types of modified atmospheres (MA: MA A: 70%O(2)+30%CO(2); MA B: 69.3%N(2)+30%CO(2)+0.7%CO) at 7, 14 and 21 d post-packaging. The lowest pH was found in G4 and in G5. The highest WHC and the lowest CL were found in G2 and G3 groups (P<0.05). Modified atmospheres did not affect on pH, WHC, CL and DL, although a significant effect (P<0.001) on colour was found at all the analysis times. Both the type of stunning and the modified atmosphere affected SF values.

Physiological responses of Manchega suckling lambs: Effect of stunning with different CO(2) concentrations and exposure times.

Four groups of Manchega breed suckling lambs, stunned with different CO(2) concentrations and exposure times (G1: 80% CO(2) 90s; G2: 90% CO(2) 90s; G3: 90% CO(2) 60s; G4: 80% CO(2) 60s) plus an electrically stunned control group (G5), were used to determine (1) the physiological responses (hormonal, haematological and biochemical blood parameters) of animals after stunning and (2) the stunning effectiveness in each group. No significant differences were found among groups for hormonal levels. Within haematological parameters, significant differences among groups were only found for haemoglobin (P<0.05) and leucocytes (P<0.01), with lowest values in both groups stunned with 90% CO(2). There were significant differences between groups (P<0.01) for urea and total protein, creatinine and LDH (P<0.05; lowest and highest, respectively, for G1) and for sodium (P<0.001; lowest in G3). Stunning effectiveness was highest in G5, G3 and G1 groups and lowest in G2 and G4 (100%, 90%, 89%, 50% and 43%, respectively). According to discriminant analysis, sodium, leucocytes and creatinine marked the differences among stunning groups.

Light lamb meat quality packed under modified atmospheres: effect of stunning systems (electrically v. gas).

In the present study, the effect of type of stunning (electrical v. gas) and the gas mixtures in a modified atmosphere (MA) packing system (MA-A: 30% CO2 + 70% O2; MA-B: 30% CO2 + 69.3% N2 + 0.7% CO; MA-C: 40% CO2 + 60% N2) on light lamb meat quality, obtained by 20 males of Manchego Spanish breed, was evaluated at 7, 14 and 21 days post packing. For all ageing times, gas-stunned groups reached the highest pH (P < 0.001) and shear force (SF) values (P < 0.05), and at 21 days, water losses were highest; in contrast, meat colour coordinates were lower (P < 0.001). In general, MA-B showed the highest stability for colour coordinates whereas the lowest tenderness (high SF) was found on MA-A. In this study, the gas mixture with a low carbon monoxide level (MA-B) promoted a higher colour stability and good tenderness, in comparison to the other two blends. In contrast, the conventional packaging system (type A, high oxygen levels) had a reduced tenderness and major colour instability.

Effects of stunning with different carbon dioxide concentrations and exposure times on suckling lamb meat quality.

Forty-nine Manchega breed male suckling lambs were used to determine the effect of different stunning methods (using two different CO2 concentrations and exposure times) on lamb meat quality. The lambs were allocated to five stunning treatments including four CO2 treatments [80% CO2 for 90s (G1); 90% CO2 for 90s (G2); 90% CO2 for 60s (G3); 80% CO2 for 60s (G4)] and an electrically stunned control group (G5). The gas-stunning treatments did not cause neither haematomas nor blood splash in the carcasses. Meat quality was evaluated by testing pH, colour (L(∗), a(∗), b(∗), chroma, hue values), water holding capacity (WHC), cooking loss (CL), shear force (SF), drip loss (DL) and total aerobic bacteria. Statistical differences in pH at 24h post-mortem, colour, WHC and CL were not found among groups. After 7 days post-mortem, there were statistical differences among groups in pH (highest in G4 and G5) and in DL (highest in G1). There were differences in SF due to stunning method evident after 72h and 7 days ageing. The statistical differences (P<0.01) among groups on total aerobic bacteria at 24h (lower and higher values in G2 and G5, respectively) disappeared at 7 days post-mortem. As G2 as G3, could be recommended to stunning suckling lambs since a highest stability with ageing time on meat quality was found using 90% CO2.

CO(2) stunning procedure on Manchego light lambs: Effect on meat quality.

This study examined the effect of different gas stunning methods (concentration of CO(2)/time of exposure (G1: 80%90s; G2: 90%90s; G3: 90%60s; G4: 80%60s) on the initial meat quality of Manchego breed light lambs (25kg live weight) and at 7days post-mortem, assessed by pH, colour coordinates, water holding capacity (WHC), cooking loss (CL), drip loss (DL) shear force (SF) and lipid oxidation. An electrically stunned control group (G5) was used. Stunning method had a significant effect on pH values (P<0.001) as well as on pH decline (P<0.01). The lowest pH was found at 24h post-slaughter in G1 and the highest one on G5. The greatest drop in pH (pH(0)-pH(24)) was found in G1 and G5 while the smallest in G3. In general values of colour coordinates, WHC and DL were similar in all groups. Stunning method affected CL (P<0.001) at 7 days post-slaughter, with the lowest values being found in G1. Significant differences among groups were found (P<0.05) in SF values at both post-mortem times, with less tender meat in groups stunned with 80% CO(2), especially in G1. A significant effect (P<0.001) due to the type of stunning was found at 24h on lipid oxidation, with the highest value in G5. After ageing this parameter was lowest (P<0.05) in G1 and G4.

Microbial quality and lipid oxidation of Manchega breed suckling lamb meat: Effect of stunning method and modified atmosphere packaging.

The effect of CO(2) concentration and exposure time at stunning [80% CO(2) for 90s (G1); 90% CO(2) for 90s (G2); 90% CO(2) for 60s (G3); 80% CO(2) for 60s (G4)] plus an electrically stunned control group (G5) was assessed for lipid oxidation (LO) and microbial levels, [total viable counts, lactic acid bacteria, Enterobacteriaceae and Pseudomonas spp.] in Manchega breed suckling lamb meat at 24h and 7days post-mortem. Differences in LO were found at 7days post-mortem (P<0.05) with the highest value for G4. In general, values of all microorganisms studied were higher in G5. In addition the effects of these stunning methods (TS) on both LO and microbial counts were assessed in samples packed under two different types of modified atmospheres (MA: MA-A: 70% O(2)+30% CO(2); MA-B: 69.3% N(2)+30% CO(2)+0.7% CO) at 7, 14 and 21days post-packaging. Both factors (TS and MA), significantly affected LO, which was highest in the samples from the MA-A/G4 group. In general there were no significant differences in microbial quality between modified atmospheres. However, the type of stunning affected microbial count (P<0.001) at all analysis times. In general, G4 and G5 showed the highest level in all microorganisms assessed, while the rest of the gas-stunning groups showed more stability with ageing.

Effect of stunning systems on meat quality of Manchego suckling lamb packed under modified atmospheres.

The effects of the type of stunning (TS) [electrically vs. gas] and packing in modified atmospheres (MA) [MA-A: 30% CO(2)/70% O(2); MA-B: 30% CO(2)/69.3% N(2)/0.7% CO; MA-C: 40% CO(2)/60% N(2)] on meat quality (pH), drip losses (DL), water holding capacity (WHC), shear force (SF) and instrumental colour (L(∗), and C(∗)chroma) of suckling lamb of the Spanish Manchego breed at 7, 14 and 21d post-packing was studied. Acceptance of meat samples (on the basis of colour and odour) was determined. In general neither the TS nor the MA affected the pH values. Meat from the gas stunned lambs had the lowest DL (P<0.001 at 14d post-packing), but lower WHC (more water expelled; P<0.01 at 14 and 21d post-packing), was more tender (P<0.01) and had higher L(∗) (P<0.001 at 14d post-packing) and C(∗) values (P<0.001) than the electrically stunned group. Similar values of WHC and SF were observed for all MA types but the use of CO in the packs (MA-B) caused less DL, gave the highest C(∗) values, acceptability and colour stability with time of storage.

Effect of different stunning systems on meat quality of light lamb.

The present study was proposed to compare the effect that different types of stunning (TS) had on the quality of refrigerated meat from light lambs of the Spanish Manchega breed at 24h and 7 days post-mortem. Lambs were electrically stunned (ESL; n=10), using CO(2) (GSL; n=10) or slaughtered without previous stunning (USL; n=10). Measurements on meat quality were carried out by evaluating pH, colour coordinates (L(∗), a(∗), b(∗)), water holding capacity (WHC), cooking loss (CL), shear force (SF) and drip loss (DL). At 24h post-mortem, no significant differences were found in any of the variables studied. However, at 7 days post-mortem, meat quality was affected by the different TS: pH, CL and DL were lower (P<0.001) in the USL group and GSL obtained the lowest a(∗) (redness) and b(∗) (yellowness) values (P<0.01) than in the other groups. Ageing of meat affected SF in the ESL group (P<0.01), although there were no significant differences due to treatments at any of the ageing times.

Lipid oxidation in lamb meat: Effect of the weight, handling previous slaughter and modified atmospheres.

This study examined the effect of pre-slaughter handling (electrical, gas (CO(2)) or non-stunning) on lipid oxidation (as thiobarbituric acid reactive substances, TBARS; in the unit of mg malondialdehyde/kg(-1) of meat) of Spanish Manchega breed lamb meat, at 24h and at 7 days post-mortem. Lambs were slaughtered at two different weights (light (L), 25kg, vs. suckling (S), 12.8kg). In general gas-stunned lambs had lower lipid oxidation (P<0.001), and it was higher (P<0.001) in light lambs compared to suckling lambs. In both groups (S and L), malondialdehyde level increased with time (P<0.001), although this increase was lower (P<0.05) in gas-stunned suckling lambs. In addition, we evaluated the effect of stunning methods (TS: electrical vs. gas) and the weight (L vs. S) on lipid oxidation values in samples packed in different types of modified atmosphere (MA: A: 70%O(2)+30%CO(2); B: 69.3%N(2)+30%CO(2)+0.7%CO; C: 60%N(2)+40%CO(2)) at 7, 14 and 21 days post-packing. Values were higher in samples with MA-type A and lower in B and C types (P<0.05). A significant interaction (P<0.001) weight×TS was observed and the lowest rates of TBARS were found in the samples of light lambs stunned with gas and packed under anaerobic conditions (MA-B and C).

Meat quality in suckling lambs: effect of pre-slaughter handling.

Thirty-three suckling lambs of the Manchega breed were used to compare the effects of pre-slaughter handling (PSH) on initial meat quality and at 7 days post-mortem. Lambs were distributed into three groups: electrically stunned (ESL; n=15), stunned using CO(2) (GSL; n=10) and slaughtered without previous stunning (USL; n=8). Meat quality was evaluated by examining pH, colour (L*, a*, b* values), water holding capacity (WHC), cooking loss (CL), shear force (SF) and drip loss (DL). At 24 h post-mortem, the highest pH corresponded to the USL group (P<0.05) whereas the lowest pH decline (pH(0)-pH(24)) corresponded to the GSL group (P<0.05). Differences in pH among groups disappeared after 7 days post-mortem. In general, PSH did not affect values of WHC, CL and colour parameters. After 7 days post-mortem, there were significant differences between groups in DL, this being lowest (P<0.05) in stunned animals. GSL resulted in more tender meat (lower SF value) than ESL and USL (P<0.05). Moreover, there was no blood splash in any of the carcasses of the GSL group.