Carbon stock in aboveground biomass and necromass in the Atlantic Forest: an analysis of data published between 2000 and 2021.

Synthesising knowledge on carbon stocks is an essential tool for understanding the potential of forests to store carbon and its drivers. However, such a synthesis needs to be constructed for the Atlantic Forest due to various methodological approaches and biogeographic heterogeneity. Thus, here we conducted a bibliographic search (2000 to 2021) on carbon stocks in the biomass and necromass of Atlantic Forest ecosystems to understand the variation in stocks and their explanatory variables. Drivers included spatial (altitude, forest size) and climatic (precipitation and temperature) variables, and successional stages. Based on the information in 46 articles, biomass exhibited the highest carbon stock (96%), in Mature Forests (MF), with an average of 125.34±40.3 MgC.ha-1, whereas Secondary Forests (SF) stored 82.7±38.2 MgC.ha-1. The carbon in the necromass varied from 1.63 to 11.47 MgC.ha-1, with SF exhibiting 3.90±2.73 MgC.ha-1 and MF 4.31±2.82 MgC.ha-1. Only average annual precipitation and successional stage positively explained the carbon in Atlantic Forest. This research clarifies the function and potential of Atlantic Forest fragments for storing carbon and reinforces need for conserving mature forest patches throughout the biome since one hectare of mature forest can store almost twice as much carbon as one hectare of secondary young patches.

Gluten-free diet adherence in pediatric celiac patients: Evaluating CDAT questionnaire and test for gluten detection in urine.

OBJECTIVES: This study aimed to evaluate the contributions of the Adapted Celiac Dietary Adherence Test (CDAT) and the Rapid Urinary Gluten Detection Test (u-GIP) in assessing gluten-free diet adherence in children and adolescents with celiac disease. METHODS: Fifty-four celiac patients from two pediatric gastroenterology outpatient clinics affiliated with university hospitals were evaluated. The original CDAT was adapted for children through a transcultural process, and the original cutoff point was adopted to define adherence. A single examiner carried out the u-GIP test in fresh urine samples. Sociodemographic and clinical factors and family food security status were also evaluated. RESULTS: A total of 88.9% of participants (confidence interval [CI]: 77.4-95.8; p<0.001) adhered to the gluten-free diet, as determined by the adapted CDAT score, while 87.0% (CI: 75.1-94.6; p<0.001) had negative u-GIP results. Among the 48 children adhering to the CDAT, six exhibited positive u-GIP results in a urine sample. Of the six nonadherent participants, only one had a positive u-GIP result. Notably, none of the children and adolescents with celiac disease who tested positive for u-GIP reported symptoms on the day of testing, and their growth rates remained stable. CONCLUSIONS: Even celiac children and adolescents adhering to the CDAT questionnaire may show a positive u-GIP in a single measurement without accompanying symptoms or growth impairment. The u-GIP could be helpful in complementary tests in specific situations, such as for patients who exhibit compliant behavior but still experience symptoms or maintain persistent positive serology.

EVALUATION OF THE SATISFACTION OF REMOTE TEACHING IN POSTGRADUATE COURSES IN HEALTH: SYSTEMATIC REVIEW / EVALUACIÓN DE LA SATISFACCIÓN CON LA ENSEÑANZA A DISTANCIA EN CURSOS DE POSTGRADO EN SALUD: REVISIÓN SISTEMÁTICA / AVALIAÇÃO DA SATISFAÇÃO DO ENSINO REMOTO EM CURSOS DE PÓS-GRADUAÇÃO EM SAÚDE: REVISÃO SISTEMÁTICA

Objective: To analyze and identify in the scientific literature how remote teaching is being used and how it was evaluated in postgraduate health courses. Methods: Systematic review with a search in the Virtual Health Library (VHL), with the descriptors distance education, remote learning, residency, graduate studies and health in Portuguese and English, between 2017 and 2021, in Portuguese, English and Spanish. Results: From 750 initial results, 19 articles were included. The United States leads with the largest number of publications on the subject. The evaluation of students who studied through remote teaching is positive. Conclusion: Remote teaching was introduced very quickly for a large number of specialties in the world, with this, even showing positive aspects in this type of teaching, some negative points were evident and that future improvements must be made

Objetivo: Analizar e identificar en la literatura científica el uso de la enseñanza a distancia y su evaluación en los cursos de posgrado en salud. Métodos: Revisión sistemática en las bases de datos Medline, Lilacs, BDEnf, IBECS, BBO y BDENF con los descriptores educación a distancia, aprendizaje a distancia, residencia, estudios de posgrado y salud entre 2017 y 2021, en portugués, inglés y español. Resultados: La búsqueda inicial identificó 750 resultados. En la fase de análisis de títulos y resúmenes quedaron 66 estudios. Finalmente, se incluyeron 19 artículos. Estados Unidos lidera con el mayor número de publicaciones sobre el tema. La valoración de los alumnos que estudiaron a través de la docencia a distancia es positiva. Conclusión: La enseñanza a distancia se implementó muy rápidamente para una gran cantidad de especialidades en el mundo, con lo que, aun mostrando aspectos positivos en este tipo de enseñanza, se evidenciaron algunos puntos negativos y que se deben realizar mejoras futuras

Objetivo: Analisar e identificar na literatura científica a utilização do ensino remoto e sua avaliação em cursos de pós-graduação em saúde. Métodos: Revisão sistemática nas bases de dados Medline, Lilacs, BDEnf, IBECS, BBO e BDENF com os descritores educação a distância, ensino remoto, residência, pós-graduação e saúde entre 2017 a 2021, nos idiomas português, inglês e espanhol. Resultados: A busca inicial identificou 750 resultados. Na fase de análise dos títulos e resumos sobraram 66 estudos. Por fim foram incluídos 19 artigos. Os Estados Unidos lideram com o maior número de publicações acerca do tema. A avaliação dos discentes que estudaram através do ensino remoto é positiva. Conclusão: O ensino remoto foi colocado de forma muito rápida para uma grande quantidade de especialidades no mundo, com isso, mesmo evidenciando-se aspectos positivos nesse tipo de ensino, ficou evidente alguns pontos negativos e que se deve fazer melhorias futuras.

O brincar de fazer compras como estratégia educativa em saúde bucal do ensino infantil / Playingshoppingas aneducational strategy in oral health for children in early childhood education / Jugar las comprascomo estrategia educativa ensalud bucalpara niños en educacíon infantil

Introdução:A escola é um ambiente propício para o desenvolvimento de hábitos que podem ser aplicadospor toda a vida. Nessa perspectiva, as atividades educativas com escolares podem contribuir significativamente na promoção de saúde bucal e na disseminação de bons hábitos de higiene oral, sendo alternativas capazes de instruir esses sujeitos eficientemente.Objetivo:Relatar a experiência de uma intervenção lúdica de caráter educativo realizada pela equipe de um projeto de extensão com uma turma de educação infantil em escola institucional de educação básica.Metodologia:A partir do conhecimento da faixa etária dos escolares, um jogo educativo foi desenvolvido e uma ação de caráter lúdico foi elaborada. A alimentação saudável foi a temática escolhida para ser trabalhada interdisciplinariamente à conscientização sobre o papel dos alimentos no desenvolvimento da cárie dentária e das orientações de higiene oral. Ao "brincar de fazer compras", momento em que os alunos podiam escolher quais alimentos adquirir no jogo, foi possível discorrer sobre quais dessas escolhas alimentares não eram saudáveis e também orientar os participantescom macromodelosobre a forma correta de escovação e uso dofio dental.Resultados:A interação das crianças com a atividade e com toda equipe de extensionistas foi bastante satisfatória. Foi possível verificar que os mesmos assimilaram bem os assuntos trabalhados ludicamente e foi demonstrado pela intervenção que os alunos conseguiram adquirir conhecimentos práticos sobre a saúde bucal no momento de orientação de higiene oral tanto no jogo quanto na simulação da escovação com macromodelo. Conclusões:Atividades lúdicas para escolares podem ser instrumentos capazes de transmitir conhecimentos em saúde bucal satisfatoriamente ao passo que sinalizam aspectos que devem ser desenvolvidos e incorporados no cotidiano dos indivíduos desde a mais tenra idade (AU).

Introduction: School is a conducive environment for developing habits that can be extended throughout life. From this perspective, educational activities with schoolchildren can significantly contribute to promoting oral health and to disseminate good oral hygiene habits, constituting alternatives which are capable of efficiently educating these subjects. Objective:To report the experience of a playful and educational intervention carried out by the team of an extension project with an early childhood education class in an institutional school of basic education. Methodology: An educational game was developed based on the knowledge of the age range of the school children, and a playful action was elaborated. A healthy eating theme was chosen to be worked on in an interdisciplinary way to raise awareness about the role of food in developing dental caries and oral hygiene guidelines. By "playing shopping" in which students could choose which foods to buy in a game, it was possible to discuss which of these food choices were unhealthy and also guide participants with a macromodel on the correct way of brushing and flossing. Results: The interaction of children with the activity and with the entire extension team was quite satisfactory. It was possible to verify that they had assimilated the playfully addressed issues and it was demonstrated that students were able to acquire practical knowledge about oral health during the oral hygiene orientation in both the game and in the brushing simulation with the macromodel. Conclusions:Playful activities for schoolchildren may constitute instruments which are capable of satisfactorily transmitting knowledge on oral health, and can indicate aspects which must be developed and incorporated into the daily lives of individuals from a very early age (AU).

Introducción: La escuela es un entorno favorable para el desarrollo de hábitos que pueden prolongarse a lo largo de la vida. En esta perspectiva, las actividades educativas con alumnos pueden contribuir significativamente a la promoción de salud oral y de difusión de buenas costumbres de higiene oral, siendo estas alternativas capaces de educar estos sujetos eficientemente.Objetivo:Relatar la experiencia de una intervención lúdica educativo, realizada por un equipo de un proyecto de extensión, direccionada a un grupo de educación infantil en una escuela del sistema educativo básico brasileño. Metodología: A partir del conocimiento de la franja etaria , se diseñó un juego educativo y se planeó una acción de carácter lúdico. La alimentación sana fue la temática elegida para que, de este modo, además de orientaciones de cuidados orales, también se trabajara, interdisciplinariamente, la concientización sobre la relación entre los alimentos y el surgimiento de caries dentarias. Al "jugar a las compras", momento en que los alumnos podían elegir cuales alimentos iban a adquirir, fue posible debatir sobre cuales de las opciones alimentares no eran sanas y, además de eso, orientar los participantes, con un tipodonto, sobre la forma correcta de cepillarse y de usar el hilo dental. Resultados: La interacción con la actividad y con el equipo de extensión fue muy exitosa. Fue posible verificar que los alumnos comprendieron los temas trabajados lúdicamente y también que, a través de la intervención, consiguieron alcanzar conocimientos prácticos sobre salud oral, tanto en el momento de la orientación de higiene oral cuanto en la actividad de simulación de cepillado con el apoyo del tipodonto.Conclusiones:Actividades lúdicas para niños r pueden ser instrumentos capaces de proporcionar aprendizajes sobre salud oral en la medida en que señalan aspectos que necesitan ser desarrollados en las prácticas rutineras de sujetos desde muy temprana edad (AU).

O brincar de fazer compras como estratégia educativa em saúde bucal do ensino infantil / Jugar las comprascomo estrategia educativa ensalud bucalpara niños en educacíon infantil / Playing shoppingas an educational strategy in oral health for children in early childhood education / Playing shopping as an educational strategy in oral health for early childhood education

A escola é um ambiente propício para o desenvolvimento de hábitos que podem ser aplicadospor toda a vida. Nessa perspectiva, as atividades educativas com escolares podem contribuir significativamente na promoção de saúde bucal e na disseminação de bons hábitos de higiene oral, sendo alternativas capazes de instruir esses sujeitos eficientemente. Relatar a experiência de uma intervenção lúdica de caráter educativo realizada pela equipe de um projeto de extensão com uma turma de educação infantil em escola institucional de educação básica. A partir do conhecimento da faixa etária dos escolares, um jogo educativo foi desenvolvido e uma ação de caráter lúdico foi elaborada. A alimentação saudável foi a temática escolhida para ser trabalhada interdisciplinariamente à conscientização sobre o papel dos alimentos no desenvolvimento da cárie dentária e das orientações de higiene oral. Ao "brincar de fazer compras", momento em que os alunos podiam escolher quais alimentos adquirir no jogo, foi possível discorrer sobre quais dessas escolhas alimentares não eram saudáveis e também orientar os participantescom macromodelosobre a forma correta de escovação e uso dofio dental. A interação das crianças com a atividade e com toda equipe de extensionistas foi bastante satisfatória. Foi possível verificar que os mesmos assimilaram bem os assuntos trabalhados ludicamente e foi demonstrado pela intervenção que os alunos conseguiram adquirir conhecimentos práticos sobre a saúde bucal no momento de orientação de higiene oral tanto no jogo quanto na simulação da escovação com macromodelo. Atividades lúdicas para escolares podem ser instrumentos capazes de transmitir conhecimentos em saúde bucal satisfatoriamente ao passo que sinalizam aspectos que devem ser desenvolvidos e incorporados no cotidiano dos indivíduos desde a mais tenra idade.

School is a conducive environment for developing habits that can be extended throughout life. From this perspective, educational activities with schoolchildren can significantly contribute to promoting oral health and to disseminate good oral hygiene habits, constituting alternatives which are capable of efficiently educating these subjects. Objective_To report the experience of a playful and educational intervention carried out by the team of an extension project with an early childhood education class in an institutional school of basic education. An educational game was developed based on the knowledge of the age range of the school children, and a playful action was elaborated. A healthy eating theme was chosen to be worked on in an interdisciplinary way to raise awareness about the role of food in developing dental caries and oral hygiene guidelines. By "playing shopping" in which students could choose which foods to buy in a game, it was possible to discuss which of these food choices were unhealthy and also guide participants with a macromodel on the correct way of brushing and flossing. The interaction of children with the activity and with the entire extension team was quite satisfactory. It was possible to verify that they had assimilated the playfully.

La escuela es un entorno favorable para el desarrollo de hábitos que pueden prolongarse a lo largo de la vida. En esta perspectiva, las actividades educativas con alumnos pueden contribuir significativamente a la promoción de salud oral y de difusión de buenas costumbres de higiene oral, siendo estas alternativas capaces de educar estos sujetos eficientemente. Relatar la experiencia de una intervención lúdica educativo, realizada por un equipo de un proyecto de extensión, direccionada a un grupo de educación infantil en una escuela del sistema educativo básico brasileño. A partir del conocimiento de la franja etaria , se diseñó un juego educativo y se planeó una acción de carácter lúdico. La alimentación sana fue la temática elegida para que, de este modo, además de orientaciones de cuidados orales, también se trabajara, interdisciplinariamente, la concientización sobre la relación entre los alimentos y el surgimiento de caries dentarias. Al "jugar a las compras", momento en que los alumnos podían elegir cuales alimentos iban a adquirir, fue posible debatir sobre cuales de las opciones alimentares no eran sanas y, además de eso, orientar los participantes, con un tipodonto, sobre la forma correcta de cepillarse y de usar el hilo dental. La interacción con la actividad y con el equipo de extensión fue muy exitosa. Fue posible verificar que los alumnos comprendieron los temas trabajados lúdicamente y también que, a través de la intervención, consiguieron alcanzar conocimientos prácticos sobre salud oral, tanto en el momento de la orientación de higiene oral cuanto en la actividad de simulación de cepillado con el apoyo del tipodonto. Actividades lúdicas para niños r pueden ser instrumentos capaces de proporcionar aprendizajes sobre salud oral en la medida en que señalan aspectos que necesitan ser desarrollados en las prácticas rutineras de sujetos desde muy temprana edad.

Characterization of clinical, laboratory, IL-6 serum levels, and IL-6-174 G/C genetic polymorphisms in patients with rheumatoid arthritis and Sjögren's syndrome.

OBJECTIVE: This study aimed to characterize the clinical (disease activity and exocrine gland function), laboratory, interleukin 6 (IL-6) serum levels, and IL-6-174G/C (rs1800795) genetic polymorphisms among rheumatoid arthritis (RA), RA plus Sjögren's syndrome (RA+SS), and control subjects. METHODS: A case-control study enrolling 137 women (52±11 years old) were divided into three groups as follows: RA (n=70), RA+SS (n=29), and healthy control (C, n=38). Individuals underwent clinical evaluation composed of Schirmer's test, unstimulated salivary flow rate, and evaluation of disease activity and functional capacity (Disease Activity Score [DAS28] and Health Assessment Questionnaire [HAQ]). IL-6 serum levels and IL-6-174G/C polymorphisms were assessed. RESULTS: RA and RA+SS presented higher serum levels of IL-6 than controls (p<0.001). Also, higher IL-6 levels were related to swollen joints (p=0.038), limited functional capacity (p=0.004), and disease activity (p≤0.001). However, neither IL-6-174G/C genetic polymorphism nor its allele frequency was associated with RA or RA+SS. CONCLUSION: IL-6 serum is an important marker of RA activity and functional incapacity, but IL-6-174G/C genetic polymorphism did not differ among healthy controls and cases.

The Program for Biodiversity Research in Brazil: The role of regional networks for biodiversity knowledge, dissemination, and conservation.

The Program for Biodiversity Research (PPBio) is an innovative program designed to integrate all biodiversity research stakeholders. Operating since 2004, it has installed long-term ecological research sites throughout Brazil and its logic has been applied in some other southern-hemisphere countries. The program supports all aspects of research necessary to understand biodiversity and the processes that affect it. There are presently 161 sampling sites (see some of them at Supplementary Appendix), most of which use a standardized methodology that allows comparisons across biomes and through time. To date, there are about 1200 publications associated with PPBio that cover topics ranging from natural history to genetics and species distributions. Most of the field data and metadata are available through PPBio web sites or DataONE. Metadata is available for researchers that intend to explore the different faces of Brazilian biodiversity spatio-temporal variation, as well as for managers intending to improve conservation strategies. The Program also fostered, directly and indirectly, local technical capacity building, and supported the training of hundreds of undergraduate and graduate students. The main challenge is maintaining the long-term funding necessary to understand biodiversity patterns and processes under pressure from global environmental changes.

Desafios da pandemia para a mentoria: o papel dos mentores juniores e das redes sociais / Challenges of the pandemic for mentoring: the role of junior mentors and social networks

Resumo: Introdução: A aprovação no curso de Medicina traz consigo a euforia e, por vezes, associa-se a grandes mudanças no dia a dia dos alunos. Essas mudanças estão relacionadas a momentos de medo, sofrimento e adaptação. Os programas de mentoria surgem como espaços importantes de cuidado que oferecem acolhimento e suporte às vivências dos alunos, de modo a contribuir para o desenvolvimento pessoal e acadêmico deles. Relato de experiência: Diante da necessidade de adaptar o ensino presencial ao modelo remoto, em detrimento da pandemia de Covid-19, o programa de mentoria da Universidade Federal do Rio Grande do Norte investiu na realização de atividades on-line, protagonizadas por monitores denominados mentores juniores. Essas atividades se valeram do potencial das mídias sociais, por meio das plataformas de videoconferência, além de interações nas redes sociais, para realizar os encontros e promover a manutenção do vínculo, bem como proporcionar um espaço de acolhimento e integração entre estudantes e mentores. Discussão: A partir da realização de atividades remotas, alunos e mentores mostraram-se muito participativos e satisfeitos. A atuação dos mentores juniores na elaboração dessas atividades virtuais foi um importante diferencial, possibilitando o engajamento dos mais tímidos e daqueles que tinham dificuldade em cumprir os horários presenciais. Conclusão: Mediante a observação no desenvolvimento das atividades do programa e com base na avaliação dos alunos e mentores sobre o desempenho da mentoria no semestre remoto, considera-se que é de grande valia investir no potencial das mídias sociais para impulsionar as reuniões dos grupos de mentoria, garantir a manutenção do acolhimento e suporte aos alunos, bem como para estreitar os vínculos entre os participantes. Sob esse prisma, é necessário considerar a possibilidade de adotar um modelo misto no aperfeiçoamento do programa de mentoria.

Abstract: Introduction: Being accepted for medical school brings euphoria, and is sometimes associated to major changes in the student's daily life. These changes pertain to moments of fear, suffering and adaptation. The mentoring programs, therefore, appear as essential care spaces, offering welcoming and support to students, in order to contribute to their personal and academic development. Experience Report: Faced with the need to adapt from face-to-face teaching to the remote model, to help control the Covid-19 pandemic, the Mentoring program of the Federal University of Rio Grande do Norte invested in conducting online activities led by junior mentors. These activities took advantage of the potential of social media, through video conferencing platforms, in addition to interactions on social networks, to hold the meetings and maintain the link, as well as providing a space for welcoming and integrating students and mentors. Discussion: In relation to the remote activities, students and mentors were found to be very participative and satisfied. The role of junior mentors in developing these virtual activities was an important differential, enabling the engagement of the most timid and those who had difficulty in meeting the face-to-face schedules. Conclusion: Through observation during the development of the program activities, and based on the evaluation of students and mentors on the performance of mentoring in the remote semester, the conclusion is drawn that it is important to invest in the potential of social media to boost mentoring group meetings, ensure that the students are offered reception and support, and to strengthen the bonds with the participants. In light of this, it is necessary to consider the possibility of adopting a mixed teaching model to improve the mentoring program.

EBV and CMV viral load in rheumatoid arthritis and their role in associated Sjögren's syndrome.

INTRODUCTION: The role of viral infections in the pathogenesis of autoimmune diseases has long been suggested, but little evidence is available. OBJECTIVE: This study aimed to evaluate an association between EBV and CMV and the presence of rheumatoid arthritis and its association with Sjögren's Syndrome. PATIENTS AND METHOD: A case-control study was performed with 227 patients divided in RA (n = 99), RA/SS (n = 20), and C (n = 128). Resting salivary flow rate and Schirmer's test were performed; minor salivary gland biopsy was indicated in the case of suspected Sjögren's syndrome. CMV and EBV viral loads were quantified in peripheral blood, and their presence in glandular tissue samples was evaluated by in situ hybridization (EBV) and immunohistochemistry (CMV). RESULTS: EBV was more frequent in RA and RA/SS than in C (P < .000007). No correlation with clinical markers (P > .05) or between RA and RA/SS was found (P > .05). A higher number of EBV/DNA copies were found in RA (158.52 copies/µL) and RA/SS (99.24 copies/µL) (P = .739). EBV/DNA was associated with the Schirmer test (P = .0231). CMV was detected in one patient of the RA group. None of the viruses were detected in biopsies of minor salivary glands. CONCLUSIONS: Detection of EBV/DNA in peripheral blood was associated with RA regardless of the presence of SS.

Can fallen trees enhance aboveground biomass estimation? A proposal for the Brazilian Atlantic Forest

Introduction: Estimating aboveground biomass (AGB) in protected forests is a challenge, due to high costs and legal restrictions for direct assessments, and also to frequently weak estimation provided by general AGB equations. Objective: We propose a new approach that uses dead fallen trees (DFTs) to improve AGB estimation. We aim to analyse if the adjustment of allometric models based on DFTs provides a suitable local equation for AGB estimation or helps to validate existing pantropical or regional allometric equations. Methods: The study was carried out at the Dois Irmãos State Park (PEDI), Pernambuco, Northeast Brazil. Along 4 000 m, using the line intersect technique, we sampled 37 recent dead fallen trees ranging from 7.6 to 92.3 cm in diameter and from 8.6 to 29.4 m in height. Nine models were adjusted, and the best equation (local) was compared to regional and pantropical equations. Results: Oneequation (AGBkg= 1.5292* DBH2.0601*TH-0.2187) produced biomass estimates which did not differ from the observed values (P > 0.05). Differently from the others, this equation overestimated AGB in only 2.8 % and, along with other parameters of analysis (R2 adj and Syx %) had the best overall performance. Analysing the performance of the local equationversusavailable equations for estimating AGB, we found that only the local and the pantropical equations by Pearson et al. (2005) estimated values that were not significantly different from observed values. However, the local equation is more appropriate for local estimation of AGB, since it has the lowest RSME and CV. Conclusions: Based on the proposed approach, we were able to offer a local equation for tree AGB estimation based on fallen trees. The DFT method is efficient regarding time and costs, avoiding tree logging in protected forests and helps to guide the choice of a proper equation for forest AGB demands.

Introducción: La estimación de la biomasa aérea (AGB) en los bosques protegidos es un desafío, debido a los altos costos y las restricciones legales para las evaluaciones directas, y también a una estimación con frecuencia débil proporcionada por las ecuaciones generales de AGB. Objetivo: Proponemos un nuevo enfoque que utiliza los árboles muertos caídos (DFT) para mejorar la estimación de AGB. Nuestro objetivo es responder si el ajuste de modelos alométricos basados en DFT proporciona una ecuación local adecuada para la estimación de AGB o ayuda a validar las ecuaciones alométricas pantropicales o regionales existentes. Métodos: El estudio se realizó en el Parque Estatal Dois Irmãos (PEDI), Pernambuco, Nordeste de Brasil. En el espacio de 4 000 m, utilizando la técnica de intersección de líneas, se tomaron muestras de 37 árboles muertos recientemente que van desde 7.6 a 92.3 cm de diámetro y 8.6 a 29.4 m de altura. Se ajustaron nueve modelos y se comparó la mejor ecuación (local) con las ecuaciones regionales y pantropicales. Resultados: Una ecuación (AGBkg = 1.5292 * DBH2.0601 * TH-0.2187) determinó estimaciones de biomasa que no diferían de los valores observados (p> 0.05). A diferencia de las otras, esta ecuación sobreestimó AGB en solo 2.8 % y, junto con otros parámetros de análisis (R2 adj y Syx %), presentó el mejor rendimiento general. Analizando el rendimiento de la ecuación local versus las ecuaciones disponibles para estimar AGB, encontramos que solo las ecuaciones locales y pantropicales de Pearson et al. (2005) estimaron valores que no fueron significativamente diferentes de los valores observados. Sin embargo, la ecuación local es más apropiada para la estimación local de AGB, ya que tiene el RSME y CV más bajos. Conclusiones: Con base en el enfoque propuesto, pudimos proponer una ecuación local para la estimación de AGB basada en árboles caídos. La estimación basada en DFT es eficiente en términos de tiempo y costo, evita la tala de árboles en bosques protegidos y ayuda a guiar la elección de una ecuación adecuada para las demandas de AGB forestales.

Anátomo-radiografia dos ossos longos do membro torácico do Tamandua tetradactyla / Anatomo-radiography of long-bones in thoracic limb of Tamandua tetradactyla

Objetivou-se descrever os acidentes anatômicos dos ossos longos do membro torácico de Tamandua tetradactyla por meio de análises macroscópicas e radiográficas. Foram utilizados 34 espécimes, que foram a óbito por atropelamento. A avaliação do úmero demonstrou as mesmas estruturas presentes nos animais domésticos, além de outras sem prévia descrição. Já no antebraço, rádio e ulna se apresentaram completamente separados, e apesar de, como o úmero, serem identificados alguns dos mesmos acidentes anatômicos descritos em outras espécies, também notamos particularidades. Todas as estruturas descritas na análise macroscópica foram identificadas à radiografia, quando realizada em pelo menos duas projeções ortogonais. As estruturas anátomo-radiográficas dos ossos longos do T. tetradactyla demonstraram grande variaçãoanatômica em comparação a outros mamíferos, o que torna a referida espécie muito singular. Assim o conhecimento de suas particularidades é fundamental para abordagens clínico-cirúrgicas mais seguras.

The objective was to describe the anatomical accidents of the long bones of the thoracic limb of Tamandua tetradactyla by means of macroscopic and radiographic analyzes. We used 34 specimens, which were death by running over. Evaluation of the humerus demonstrated the same structures present in domestic animals, besides others without previous description. In the forearm, radius and ulna were completely separated, and although, like the humerus, some of the same anatomical accidents described in other species were identified, we also noticed particularities. All the structures described in the macroscopic analysis were identified on radiography, when performed in at least two orthogonal projections. The anatomic-radiographic structures of the long bones of T. tetradactyla showed great anatomical variation compared to other mammals, which makes the species very unique. Thus knowledge of their particularities is fundamental for safer clinical-surgical approaches.

Anátomo-radiografia dos ossos longos do membro torácico do Tamandua tetradactyla / Anatomo-radiography of long-bones in thoracic limb ofTamandua tetradactyla

Objetivou-se descrever os acidentes anatômicos dos ossos longos do membro torácico de Tamandua tetradactyla por meio de análises macroscópicas e radiográficas. Foram utilizados 34 espécimes, que foram a óbito por atropelamento. A avaliação do úmero demonstrou as mesmas estruturas presentes nos animais domésticos, além de outras sem prévia descrição. Já no antebraço, rádio e ulna se apresentaram completamente separados, e apesar de, como o úmero, serem identificados alguns dos mesmos acidentes anatômicos descritos em outras espécies, também notamos particularidades. Todas as estruturas descritas na análise macroscópica foram identificadas à radiografia, quando realizada em pelo menos duas projeções ortogonais. As estruturas anátomo-radiográficas dos ossos longos do T. tetradactyla demonstraram grande variação anatômica em comparação a outros mamíferos, o que torna a referida espécie muito singular. Assim o conhecimento de suas particularidades é fundamental para abordagens clínico-cirúrgicas mais seguras.

The objective was to describe the anatomical accidents of the long bones of the thoracic limb of Tamandua tetradactyla by means of macroscopic and radiographic analyzes. We used 34 specimens, which were deaths by running over. Evaluation of the humerus demonstrated the same structures present in domestic animals, besides others without previous description. In the forearm, radius and ulna were completely separated, and although, like the humerus, some of the same anatomical accidents described in other species were identified, we also noticed particularities. All the structures described in the macroscopic analysis were identified on radiography, when performed in at least two orthogonal projections. The anatomic-radiographic structures of the long bones of T. tetradactyla showed great anatomical variation compared to other mammals, which makes the species very unique. Thus knowledge of their particularities is fundamental for safer clinical-surgical approaches.

Biodiversity of aquatic environments in a peri-urban Atlantic Forest protected remnant: a checklist / Biodiversidade de ambientes aquáticos em remanescente protegido da Mata Atlântica periurbana: um checklist

Abstract: We present a checklist for the aquatic biodiversity from two reservoirs within a PPBio (Biodiversity Research Program) site in a peri-urban forest fragment, the Dois Irmãos State Park (PEDI), in Pernambuco, Brazil. We obtained the data via extensive field collection and information from a specialized literature survey. We recorded 397 species in 156 families; the animal was the most abundant group (140 species) followed by fungi taxa (103), periphyton (69), aquatic macrophytes (44), and terrestrial plants in flooded areas (41). This review reflects different sample efforts toward selected groups and allows the definition of a long-term protocol for guiding new research based on the identified knowledge gaps revealed. Future ecological research should address the influence of the trophic state of the reservoirs, as well as the effects of competitive exclusion and predation on the long-term viability of the local diversity.

Resumo: Apresentamos uma lista da biodiversidade aquática de dois reservatórios em um sítio do PPBio (Programa de Pesquisa em Biodiversidade) em um fragmento de floresta peri-urbana, o Parque Estadual Dois Irmãos (PEDI), em Pernambuco, Brasil. Obtivemos os dados através de extensa coleta de campo e informações de pesquisa bibliográfica especializada. Registramos 397 espécies em 156 famílias; os animais foram o grupo mais abundante (140 espécies), seguido pelos fungos (103), perifíton (69), macrófitas aquáticas (44) e plantas terrestres em áreas alagadas (41). Esta revisão reflete diferentes esforços de amostra para grupos selecionados e permite a definição de um protocolo de longo prazo para orientar novas pesquisas com base nas lacunas de conhecimento identificadas. Pesquisas ecológicas futuras devem abordar a influência do estado trófico dos reservatórios, bem como os efeitos da exclusão competitiva e predação na viabilidade a longo prazo da diversidade local.

Integrating trait and evolutionary differences untangles how biodiversity affects ecosystem functioning.

Biodiversity and ecosystem functioning (BEF) research advocates that biodiversity loss has a drastic alteration on ecosystem functioning. However, studies have barely investigated how the evolutionary dependence of species traits affects EF. Here, we developed an integrated approach combining functional (FD) and phylogenetic diversity (PD) into a single space to disentangle the effects of diversity on leaf decomposition. We conducted an experiment manipulating plant leaves into litterbags containing four species (from a pool of 27) combined in four different treatments represented by low or high FD and PD; these treatments present different scenarios of trait evolution and, therefore, a treatment with high FD and low PD, for instance, mimics a community assembled by divergent trait evolution of close relatives. We found that leaf decomposition was 30% slower in pools with high FD and PD. We show species pool with higher FD and PD have non-additive effects on decomposition, which means there is a negative effect of mixtures combining species with great functional and evolutionary differences. In addition, interactive effects of PD and FD were more important to leaf decomposition than their isolated effects. Our results suggest that PD and FD have interactive effects on decomposition and represent different axes of ecosystem variation, indicating we should avoid using phylogenies as a proxy for functional diversity. We argue that future BEF experiments may alter their design by considering a multifaceted scenario investigating community effects on ecosystem functioning, and idiosyncratic effects of key traits which may determine community assembly and ecosystem processes.

Ability of two new thiazolidinediones to downregulate proinflammatory cytokines in peripheral blood mononuclear cells from children with asthma

Allergic asthma is a chronic, complex inflammatory disease of the airway. Despite extensive studies on the immunomodulation of T helper (Th) cell pathways (i.e., Th1 and Th2) in asthma, little is known about the effects of Th17 pathway modulation, particularly that involving peroxisome proliferator-activated receptors (PPARs). In response, two new thiazolidinedione derivatives-namely, LPSF-GQ-147 and LPSF-CR-35 were synthesized and evaluated for their immunomodulatory effects on Th17-related cytokines, including interferon γ (IFNγ), interleukin IL-6, IL-17, and IL-22 in the peripheral blood mononuclear cells of asthmatic children. Both compounds were nontoxic even at high concentrations (i.e., 100 µM). The LPSF-CR-35 compound significantly reduced the levels of IL-17A (p = .039) and IFNγ (p = .032) at 10 µM. For IL-22 and IL-6, significant reduction occurred at 100 µM (p = .039 and p = .02, respectively). Conversely, LPSF-GQ-147 did not significantly inhibit the production of the tested cytokines, the levels of all of which were more efficiently reduced by LPSF-CR-35 than methylprednisolone, the standard compound. Real-time polymerase chain reaction assay confirmed that LPSF-GQ-147 has significant PPARγ modulatory activity. Such data indicate that both LPSF-CR-35 and LPSF-GQ-147 are promising candidates as drugs for treating inflammation and asthma

DOAÇÃO DE ÓRGÃOS E TECIDOS PARA TRANSPLANTES: IMPASSES SUBJETIVOS DIANTE DA DECISÃO FAMILIAR / ORGAN AND TISSUE DONATION FOR TRANSPLANTATION: SUBJECTIVE IMPASSES BEFORE THE FAMILY DECISION / DONACIÓN DE ORGÁNOS Y TEJIDOS PARA TRASPLANTES: IMPASSES SUBJETIVOS ANTE LA DECISIÓN FAMILIAR

Apesar dos investimentos no desenvolvimento dos transplantes no estado da Bahia, ainda prevalece um índice de 70% de negativa familiar para doação de órgãos e tecidos para transplantes. Essa realidade motiva inúmeras considerações. O objetivo deste artigo foi revelar os impasses subjetivos intervenientes em familiares e profissionais que participaram do processo de doação de órgãos e tecidos para transplantes. Trata-se de um estudo qualitativo, no qual foram construídos dez casos por meio de entrevistas e diários de campo referentes à observação de familiares e profissionais que participaram do processo de decisão sobre a doação. A análise e discussão demonstrou aspectos relevantes que interferem na escolha familiar sobre a doação: a falta de informação sobre o tema, crenças culturais e religiosas, a maneira como é conduzida a entrevista familiar para doação, dificuldade para compreender a morte encefálica, dentre outros. As considerações finais indicaram que o tempo para a família tomar a decisão é um fator significativo que pode contribuir para o alto índice de negativa familiar para doação. A família precisa ser acolhida desde a abertura do protocolo de morte encefálica e a entrevista familiar para doação deve possibilitar uma dimensão terapêutica que vise a amenizar a dor da família.

Despite investments in the development of transplants in the State of Bahia, a 70% family refusal index for organ and tissue donation for transplants still prevails. This reality motivates countless considerations. The objective of this article is to reveal the subjective impasses involved in family members and professionals who participated in the process of organ and tissue donation for transplants. This is a qualitative study, in which ten cases were constructed through interviews and field journals referring to the observation of family members and professionals who participated in the donation decision process. The analysis and discussion showed relevant aspects that interfere in the family choice on the donation: lack of information about the theme, cultural and religious beliefs, the way in which the family interview for donation is conducted, difficulty in understanding brain death, among others. The final considerations indicated that the time for the family to make the decision is a significant factor that may contribute to the high rate of family negative for donation. The family needs to be welcomed since the opening of the brain death protocol and the family interview for donation should provide a therapeutic dimension aimed at reducing the pain of the family.

A pesar de las inversiones en el desarrollo de los trasplantes en el Estado de Bahía, aún prevalece un índice de 70% de negativa familiar para donación de órganos y tejidos para trasplantes. Esta realidad motiva numerosas consideraciones. El objetivo de este artículo es revelar los impasses subjetivos intervinientes en familiares y profesionales que participaron en el proceso de donación de órganos y tejidos para trasplantes. Se trata de un estudio cualitativo, en el cual se construyeron diez casos por medio de entrevistas y diarios de campo referentes a la observación de familiares y profesionales que participaron en el proceso de decisión sobre la donación. El análisis y discusión demostró aspectos relevantes que interfieren en la elección familiar sobre la donación: la falta de información sobre el tema, creencias culturales y religiosas, la manera como se conduce la entrevista familiar para donación, dificultad para comprender la muerte encefálica, entre otros. Las consideraciones finales indicaron que el tiempo para la familia tomar la decisión es un factor significativo que puede contribuir al alto índice de negativa familiar para donación. La familia necesita ser acogida desde la apertura del protocolo de muerte encefálica y la entrevista familiar para donación debe posibilitar una dimensión terapéutica que tenga como objetivo amenizar el dolor de la familia.

Fibromatose agressiva (tumor desmoide) associada ao implante mamário: revisão da literatura e apresentação de três novos casos / Aggressive fibromatosis (desmoid tumor) associated with breast implant: literature review and presentation of three new cases

INTRODUÇÃO: As fibromatoses consistem na proliferação de tecido fibroso, na forma de faixas ou nódulos, associadas às fáscias e aponeuroses. Sua variedade agressiva, denominada previamente tumor desmoide, se comporta de forma semelhante a uma neoplasia maligna, com destruição local de tecidos, sem, entretanto, originar metástases. MÉTODOS: Fez-se revisão da literatura de1979 a janeiro de 2017. Dados de três pacientes, operados entre maio de 2010 e agosto de 2015, foram revistos, com proservação até março de 2017. Observaram-se idade, características do implante, via de introdução do mesmo, tempo decorrido entre o implante e o aparecimento da fibromatose, tratamento cirúrgico instituído, acompanhamento clínico e resultados. RESULTADOS: Foram encontrados 24 trabalhos na literatura disponível, versando sobre fibromatose agressiva da parede torácica associada ao implante mamário de silicone, nos quais foram relatados 34 casos. São apresentados três casos de fibromatose agressiva associados ao implante mamário de silicone. Os casos foram tratados com sucesso por ressecção alargada da parede torácica, incluindo pele, musculatura, costelas, fáscia endotorácica e pleura parietal. A reconstrução foi bem-sucedida, realizada com tela aloplástica (Prolene & reg;) recoberta por retalho muscular em dois casos e retalho cutâneo local em um caso. CONCLUSÃO: A associação de fibromatose agressiva e implante mamário é rara. O tratamento deve consistir em cirurgia alargada, removendo-se o implante e toda a área da cápsula adjacente, em conjunto com parte da mama, costelas subjacentes, musculatura intercostal, fáscia endotorácica e pleura parietal. A reconstrução deve ser feita com tela aloplástica associada a retalho muscular ou retalho tegumentar local.

INTRODUCTION: Fibromatoses consists of the proliferation of fibrous tissue, in the form of bands or nodules, associated with fasciae and aponeuroses. The aggressive variety, previously denominated desmoid tumor, behaves similarly to malignant neoplasm, with local destruction of tissues, without, however, producing metastases. METHODS: A literature review was carried out from 1979 to January 2017. Data from three patients, operated between May 2010 and August 2015, were reviewed. Age, implant characteristics, route of introduction, time elapsed between implantation and fibromatosis, surgical treatment, clinical follow-up and results were observed. RESULTS: Twenty-four papers were found in the literature reporting aggressive fibromatosis of the chest wall associated with silicone breast implant. In these studies, 34 cases were reported. Three new cases of aggressive fibromatosis associated with silicone breast implant are now presented. These cases were successfully treated by extensive resection of the chest wall, including skin, musculature, ribs, endothoracic fascia, and parietal pleura. The reconstruction was successful, performed with alloplastic mesh (Prolene & reg;) covered by muscular flap in two cases and local skin flap in one case. CONCLUSION: The association of aggressive fibromatosis and breast implant is rare. The treatment should consist of extensive surgery, removing the breast implant and the entire area of the capsule around it, part of breast, together with the underlying ribs, intercostal muscles, endothoracic fascia and parietal pleura. The reconstruction should be made with an alloplastic mesh, covered by muscular flaps or local skin flap.

Synthesis and Anticancer Evaluation of Thiazacridine Derivatives Reveals New Selective Molecules to Hematopoietic Neoplastic Cells.

AIM AND OBJECTIVE: Cancer has become one of the leading causes of morbidity and mortality worldwide. Limitations associated with existing agents increase the need to develop more effective anticancer drugs to improve the therapeutic arsenal available. The aim of this study was to synthesize and evaluate the antiproliferative effects of three new thiazacridine derivatives. MATERIAL AND METHODS: Using a three steps synthesis reaction, three novel thiazacridine derivatives were obtained and characterized: (Z)-5-acridin-9-ylmethylene-3-(4-methyl-benzyl)-4-thioxo-thiazolidin- 2-one (LPSF/AC-99), (Z)-5-acridin-9-ylmethylene-3-(4-chloro-benzyl)-4-thioxo-thiazolidin-2- one (LPSF/AC-119) and (Z)-5-acridin-9-ylmethylene-3-(3-chloro-benzyl)-4-thioxo-thiazolidin-2- one (LPSF/AC-129). Toxicity and selectivity assays were performed by colorimetric assay. Then, changes in cell cycle and cell death induction mechanisms were assessed by flow cytometry. RESULTS: All compounds exhibited cytotoxicity to Raji (Burkitt's lymphoma) and Jurkat (acute T cell leukemia) cells, where LPSF/AC-119 showed best IC50 values (0.6 and 1.53 µ M, respectively). LPSF/AC-129 was the only cytotoxic compound in glioblastoma cell line NG97 (IC50 = 55.77 µ M). None of the compounds were toxic to normal human cells and induced neoplastic cell death primarily by apoptosis. CONCLUSION: All derivatives were more cytotoxic to hematopoietic neoplastic cells when compared to solid tumor derived cells. All three compounds are promising for in vivo and combination therapy studies against cancer.

Renal biochemistry variables and ultrasonographic imaging in healthy Squirrel monkeys (Saimiri collinsi).

BACKGROUND: The combined use of renal biochemistry and ultrasonographic imaging may improve the correct management of renal disease. Although renal disease is frequently observed in nonhuman primates, renal function markers have not yet been studied in Squirrel monkeys (Saimiri collinsi). OBJECTIVES: The aim of this study was to establish normal renal biochemistry variables and ultrasonographic features in Squirrel monkeys. MATERIAL AND METHODS: Renal biochemistry variables and ultrasonographic images were documented in 29 healthy Squirrel monkeys (15 males and 14 females). Urea, serum creatinine (SCr), and uric acid (UA) concentrations were measured by kinetic assay. Cystatin C (CysC) was analyzed by immunonephelometry. A multiple frequency linear array probe (5-12 MHz) was used for ultrasonographic imaging. The studied indicators of renal function were related to sex, age, and body mass. RESULTS: Serum creatinine was influenced by sex and body mass. Serum concentration of urea, UA, and CysC were not influenced by sex, age, and body mass. Ultrasonographic images provided accurate and comprehensive data for making clinical decisions for Squirrel monkeys. The total renal volume was only influenced by the body mass nested in sex and was positively correlated to body mass. Right renal volume was bigger than the left one. CONCLUSION: Normative standards for the renal evaluation, including biochemistry and ultrasonography, in the Squirrel monkey have been established correlated to age, sex, and body mass.

Effect of Xerostomia on the Functional Capacity of Subjects with Rheumatoid Arthritis.

OBJECTIVE: To evaluate the intensity of xerostomia and hyposalivation in subjects with rheumatoid arthritis (RA) as well as the effects of these conditions on functional incapacity and disease activity. METHODS: The study sample comprised 236 individuals of both sexes who had RA. All the individuals were submitted to clinical evaluation and unstimulated sialometry. Functional capacity was determined by using the Health Assessment Questionnaire (HAQ), xerostomia was assessed using the Xerostomia Inventory, and disease activity was evaluated with the 28-joint Disease Activity Score (DAS28). The effect of Sjögren syndrome (SS) was analyzed, and the sample was divided into 2 groups: RA (191 subjects) and RA/SS (45 subjects). RESULTS: The Xerostomia Inventory showed positive and significant correlation with fatigue (r = 0.243; p < 0.0001), number of painful joints (r = 0.218; p = 0.001), HAQ (r = 0.279; p < 0.0001), and DAS28 (r = 0.156; p < 0.0001). On regression analysis, both xerostomia (OR 3.89, 95% CI 1.84-8.23, p < 0.001) and DAS28 (for severe disease activity: OR 13.26, 95% CI 3.15-55.79, p < 0.001) showed influence on functional incapacity. Forty-five individuals (19.1%) presented with secondary SS, and having this diagnosis was not associated with disease activity or functional capacity. CONCLUSION: Xerostomia demonstrated an adverse effect on quality of life of subjects with RA, being associated with a reduction in functional capacity. In this clinical setting, xerostomia can be monitored as a marker of worse clinical evolution.