An integrated metabolome and transcriptome analysis of the Hibiscus syriacus L. petals reveal the molecular mechanisms of anthocyanin accumulation.

Hibiscus syriacus L. var. Shigyoku is a new double-flowered bluish-purple variety in China that changes color during flower development from bluish-purple to light purple. There is limited information on the anthocyanin accumulation patterns and associated transcriptome signatures in Shigyoku from D1 (bud) to open flower (D3). Here, we employed a combined transcriptome and metabolome approach to understanding the mechanism of this color change. Our results demonstrate that cyanidins, pelargonidins, delphinidins, petunidins, peonidins, and malvidins were differentially accumulated in Shigyoku petals. The anthocyanin biosynthesis started in D1, was significantly upregulated in D2 (semi-open flower), and reduced in D3. However, malvidins, pelargonidins, and peonidins could be associated with the bluish-purple coloration on D2. Their reduced accumulation in D3 imparted the light purple coloration to Shigyoku petals on D3. Significant contributions in the color change could be associated with the expression changes in anthocyanin biosynthesis genes i.e., LARs, ANSs, DFRs, UGT79B1, C3'Hs, 3ATs, and BZ1s. The UFGTs were associated with the higher accumulation of glycosylated anthocyanins in D2 and D3. Furthermore, the changes in the expressions of the MYB and bHLH transcription factors were consistent with the anthocyanin accumulation. Finally, we discussed the possible roles of Jasmonic acid, auxin, and gibberellic acid signaling in regulating the MBW complex. Taken together, we conclude that H. syriacus petal coloration is associated with anthocyanin biosynthesis genes, the MBW complex, and phytohormone signaling.

Metabolic and transcriptomic analysis related to flavonoid biosynthesis during the color formation of Michelia crassipes tepal.

Michelia crassipes is the only plant with purple flowers amongst Michelia species, and its tepals exhibit an obvious color change from green to purple. In this study, a combination of metabolic and transcriptomic analyses was conducted at three stages of tepals in Michelia crassipes: green tepal, purple spot-containing tepal, and totally purple tepal. Several classes of flavonoid compounds were detected and cyanidin 3-rutinoside and delphinidin 3-glucoside were the major anthocyanins underlying the purple color formation, along with co-pigmentation of flavone compounds represented by luteolin derivatives and flavonol compounds represented by kaempferol and quercetin derivatives. Transcriptome analysis revealed up-regulation of genes encoding enzymes involved in the conversion of phenylpropanoid for flavonoid biosynthesis in Stage 1 vs. Stage 2, whereas up-regulation of most flavonoid biosynthesis genes was observed in Stage 1 vs. Stage 3. MYB, bHLH, and WD40 isoforms, as well as other classes of transcriptional factors, also exhibited differential expression. In addition, differentially expressed genes putatively related to the transport of flavonoids were also identified. The results of the current study provide insight into the regulatory mechanism underlying the color transition from green to purple in Michelia crassipes tepals and describe a complicated network involving PAL, transporter genes, and transcription factors, specifically responsible for the emergence of purple color in Stage 1 vs. Stage 2.

The identification of novel PMADS3 interacting proteins indicates a role in post-transcriptional control.

PMADS3, a known MADS-box transcriptional factor and a C-class gene for floral development, plays dual roles in controlling the identity of inner floral organs and the termination of flower meristems in petunia. In this study, it was confirmed by bimolecular fluorescence complementation (BiFC) assays that the PMADS3 protein can interact individually with E-class proteins FBP2, FBP5, FBP9 and PMADS12. A yeast two-hybrid cDNA library was screened using the entire PMADS3 as bait, and this identified further potential interaction candidates. Two novel genes, PheIF3f and PhAGO10, were isolated, and suggested to regulate mRNA and translational processes according to the analysis of protein functional domains and subcellular localization predictions. Notably, the PhAGO10 protein belongs to the Argonaute family, members of which are major players in small-RNA-guided gene silencing processes via mRNA cleavage or translational inhibition. The results of yeast two-hybrid and BiFC assays indicated that PheIF3f and PhAGO10 could interact with PMADS3. Our findings indicate that the C-class gene PMADS3 potentially participates in post-transcriptional control, as well as transcriptional regulation.