Construction of functionalized In-based metal organic framework/BiOCl1-xIx Z-scheme heterojunction for efficient photocatalytic degradation of tetracycline: Performance and mechanism.

The environmental implications of antibiotics have drawn widespread attention. Numerous monomer-based bismuth oxide halide catalysts have been extensively studied to remove tetracycline (TC) from aquatic environments. Integrating bismuth oxide halide composites with In-based metal organic framework (NH2-MIL-68(In)) might potentially serve as a novel strategy. By meticulously adjusting Cl and I within the composite bismuth halide oxide (B-x), a suite of purpose built heterojunctions (NMB-x) were synthesized, which were engineered to facilitate the efficient photodegradation of TC in simulated and actual aquatic environments. The incorporation of Z-scheme heterojunctions yielded a significant enhancement in photocatalytic responsiveness and charge carrier separation. Notably, NMB-0.3 demonstrated remarkable TC removal efficiency of 88.52 ± 3.05%, which is 3.74 times of B-0.3 within 90 min. The apparent quantum yield was also increased from 8.97% (B-0.3) to 19.68% (NMB-0.3). The removal of TC from natural water bodies was also assessed. Moreover, the photocatalyst concentration, assessed using response surface method, was found to show influential factors on TC removal. In addition, density functional theory (DFT) simulations were employed to identify vulnerable sites within TC. Intermediates and pathways in the photodegradation of TC have also been inferred. Furthermore, a comprehensive environmental toxicity assessment of representative intermediates demonstrated that these intermediates exhibited significantly reduced environmental toxicity compared to TC. This study provides a new approach to the design strategy of efficient and environmentally friendly MOF-based photocatalysts.

Innovative inbuilt moving bed biofilm reactor for nitrogen removal applied in household aquarium.

An innovative inbuilt moving bed biofilm reactor (MBBR) was created to protect fish from nitrogen in a household aquarium. During the 90 experimental days, the ammonia nitrogen (NH4+-N) concentration in the aquarium with the inbuilt MBBR was always below 0.5 mg/L, which would not threaten the fish. Concurrently, nitrite and nitrate nitrogen concentrations were always below 0.05 mg/L and 4.5 mg/L, respectively. However, the blank contrast aquarium accumulated 1.985 mg/L NH4+-N on the 16th day, which caused the fish to die. The suspended biofilms could achieve the specific NH4+-N removal rate of 45.43 g/m3/d. Biofilms presented sparsely with filamentous structures and showed certain degrees of roughness. The bacterial communities of the suspended biofilms and the sediment were statistically different (p < 0.05), reflected in denitrifying and nitrifying bacteria. In particular, the relative abundance of Nitrospira reached 1.4%, while the genus was barely found in sediments. The suspended biofilms showed potentials for nitrification function with the predicted sequence numbers of ammonia monooxygenase [1.14.99.39] and hydroxylamine dehydrogenase [EC:1.7.2.6] of 220 and 221, while the values of the sediment were only 5 and 1. This study created an efficient NH4+-N removal inbuilt MBBR for household aquariums and explored its mechanism to afford a basis for its utilization.

The effect of metformin on senescence of T lymphocytes.

BACKGROUND: Immunosenescence occurs as people age, leading to an increased incidence of age-related diseases. The number of senescent T cells also rises with age. T cell senescence and immune response dysfunction can result in a decline in immune function, especially in anti-tumor immune responses. Metformin has been shown to have various beneficial effects on health, such as lowering blood sugar levels, reducing the risk of cancer development, and slowing down the aging process. However, the immunomodulatory effects of metformin on senescent T cells still need to be investigated. METHODS: PBMCs isolation from different age population (n = 88); Flow Cytometry is applied to determine the phenotypic characterization of senescent T lymphocytes; intracellular staining is applied to determine the function of senescent T cells; Enzyme-Linked Immunosorbent Assay (ELISA) is employed to test the telomerase concentration. The RNA-seq analysis of gene expression associated with T cell senescence. RESULTS: The middle-aged group had the highest proportion of senescent T cells. We found that metformin could decrease the number of CD8 + senescent T cells. Metformin affects the secretion of SASP, inhibiting the secretion of IFN-γ in CD8 + senescent T cells. Furthermore, metformin treatment restrained the production of the proinflammatory cytokine IL-6 in lymphocytes. Metformin had minimal effects on Granzyme B secretion in senescent T cells, but it promoted the production of TNF-α in senescent T cells. Additionally, metformin increased the concentration of telomerase and the frequency of undifferentiated T cells. The results of RNA-seq showed that metformin promoted the expression of genes related to stemness and telomerase activity, while inhibiting the expression of DNA damage-associated genes. CONCLUSION: Our findings reveal that metformin could inhibit T cell senescence in terms of cell number, effector function, telomerase content and gene expression in middle-aged individuals, which may serve as a promising approach for preventing age-related diseases in this population.

Aquatic plant allelochemicals inhibit the growth of microalgae and cyanobacteria in aquatic environments.

Excess nitrogen and phosphorus nutrients in the aquatic environment result in the growth of algal cells and water eutrophication, which adversely affect the aquatic environment and human health. Therefore, discovering a safe and efficient algae suppression method is necessary to ensure the ecological safety of water. Recently, the allelopathic effects of aquatic plants on algae have attracted extensive attention from researchers. This review demonstrates the current research hotspot of allelopathic algal inhibition in aquatic plants and lists the common aquatic plant species and allelochemicals. In addition, the inhibition mechanism of allelochemicals from aquatic plants on algae is systematically discussed. Moreover, the key factors affecting the inhibition of allelopathy in algae, such as pH, temperature, algal cell density, and concentration of allelochemicals, are summarized. The present utilization modes of allelochemicals on algae are also presented. Finally, the problems existing in the study of allelopathic algal inhibition of aquatic plants are highlighted, and suggestions for further research are proposed.

Synthesis of metalla-dual-azulenes with fluoride ion recognition properties.

Azulene-based conjugated systems are of great interests due to their unusual structures and photophysical properties. Incorporation of a transition metal into azulene skeleton presents an intriguing opportunity to combine the dπ-pπ and pπ-pπ conjugated properties. No such metallaazulene skeleton however has been reported to date. Here, we describe our development of an efficient [5 + 2] annulation reaction to rapid construction of a unique metal-containing [5-5-7] scaffold, termed metalla-dual-azulene (MDA), which includes a metallaazulene and a metal-free organic azulene intertwined by sharing the tropylium motif. The two azulene motifs in MDA exhibit distinct reactivities. The azulene motif readily undergoes nucleophilic addition, leading to N-, O- and S-substituted cycloheptanetrienyl species. Demetalation of the metallaazulene moiety occurs when it reacts with nBu4NF, which enables highly selective recognition of fluoride anion and a noticeable color change. The practical [5 + 2] annulation methodology, facile functional-group modification, high and selective fluoride detection make this new π-conjugated polycyclic system very suitable for potential applications in photoelectric and sensing materials.

Synergistic Effects of Environmental Relative Humidity and Initial Water Content of Recycled Concrete Aggregate on the Improvement in Properties via Carbonation Reactions.

Moisture is the basis of CO2 transport and carbonation reactions in the internal pores of cement-based materials. Too much or too little moisture influences the effect of the carbonation modification of CO2 on recycled concrete aggregate (RCA). During the carbonation reaction process of RCA, moisture is mainly derived from the environmental relative humidity (RH) and the initial water content (IWC) of the RCA itself. According to the available literature, most of the studies on the effect of moisture on the carbonation modification of RCA considered either RH or IWC. Further investigations of the synergistic effects of RH and IWC on the improvement in the properties of carbonated recycled concrete aggregate (CRCA) are needed. In this study, accelerated carbonation experiments were conducted for RCA samples with different IWCs under different environmental RHs. The results showed that the best moisture conditions for CRCA property improvement were confirmed as RH = 70% for the dry-state IWC and RH = 50% for the saturated-state IWC. When the RCAs were carbonized under the conditions of high RH with low IWC and low RH with high IWC, CO2 had good abilities to permeate and diffuse, with the improvement in CRCA properties achieving excellent levels of performance.

Essential role of bromodomain proteins in renal cell carcinoma (Review).

Histone alterations are a hallmark of kidney cancer. Histone acetylation modification mediated by bromodomain proteins (BRD) has been indicated to be related to a variety of cancer types and several targeted inhibitors have been proven to be promising modalities for cancer adjuvant therapy. As renal cell carcinoma (RCC) is not sensitive to radiotherapy or chemotherapy, the exploration of effective adjuvant therapies remains an important research direction for advanced RCC. At present, studies on bromodomain family proteins in RCC are limited and the roles of bromodomain family proteins in RCC have remained to be fully elucidated. The present review discussed the role of bromodomain family proteins in RCC, aiming to explore possible potential therapeutic targets of BRD­related drugs in this type of cancer.

Dynamic Sweep Experiments on a Heterogeneous Phase Composite System Based on Branched-Preformed Particle Gel in High Water-Cut Reservoirs after Polymer Flooding.

Heterogeneous phase composite (HPC) flooding technology that is based on branched-preformed particle gel (B-PPG) is an important technology for enhancing oil recovery in high water-cut reservoirs. In this paper, we conducted a series of visualization experiments under the condition of developed high-permeability channels after polymer flooding, with respect to well pattern densification and adjustment, and HPC flooding and its synergistic regulation. The experiments show that for polymer-flooded reservoirs, HPC flooding can significantly reduce the water cut and increase oil recovery, but that the injected HPC system mainly advances along the high-permeability channel with limited sweep expansion. Furthermore, well pattern densification and adjustment can divert the original mainstream direction, which has a positive effect on HPC flooding, and can effectively expand the sweeping range under the synergistic effect of residual polymers. Due to the synergistic effect of multiple chemical agents in the HPC system, after well pattern densification and adjustment, the production time for HPC flooding with the water cut lower than 95% was significantly prolonged. In addition, conversion schemes, in which the original production well is converted into the injection well, are better than non-conversion schemes in terms of expanding sweep efficiency and enhancing oil recovery. Therefore, for well groups with obvious high-water-consuming channels after polymer flooding, the implementation of HPC flooding can be combined with well pattern conversion and intensification in order to further improve oil displacement.

Tumor-Promoting ATAD2 and Its Preclinical Challenges.

ATAD2 has received extensive attention in recent years as one prospective oncogene with tumor-promoting features in many malignancies. ATAD2 is a highly conserved bromodomain family protein that exerts its biological functions by mainly AAA ATPase and bromodomain. ATAD2 acts as an epigenetic decoder and transcription factor or co-activator, which is engaged in cellular activities, such as transcriptional regulation, DNA replication, and protein modification. ATAD2 has been reported to be highly expressed in a variety of human malignancies, including gastrointestinal malignancies, reproductive malignancies, urological malignancies, lung cancer, and other types of malignancies. ATAD2 is involved in the activation of multiple oncogenic signaling pathways and is closely associated with tumorigenesis, progression, chemoresistance, and poor prognosis, but the oncogenic mechanisms vary in different cancer types. Moreover, the direct targeting of ATAD2's bromodomain may be a very challenging task. In this review, we summarized the role of ATAD2 in various types of malignancies and pointed out the pharmacological direction.

ß-Methyltryptamine Provoking the Crucial Role of Strictosidine Synthase Tyr151-OH for Its Stereoselective Pictet-Spengler Reactions to Tryptoline-type Alkaloids.

Strictosidine synthase (STR), the gate enzyme for monoterpenoid indole alkaloid biosynthesis, catalyzes the Pictet-Spengler reaction (PSR) of various tryptamine derivatives with secologanin assisted by "indole sandwich" stabilization. Continuous exploration with ß-methyltryptamine (IPA) stereoselectively delivered the C6-methylstrictosidines and C6-methylvincosides by enzymatic and nonenzymatic PSR, respectively. Unexpectedly, the first "nonindole sandwich" binding mode was witnessed by the X-ray structures of STR1-ligand complexes. Site-directed mutagenesis revealed the critical cryptic role of the hydroxyl group of Tyr151 in IPA biotransformation. Further computational calculations demonstrated the adjustable IPA position in STR1 upon the binding of secologanin, and Tyr151-OH facilitates the productive PSR binding mode via an advantageous hydrogen-bond network. Further chemo-enzymatic manipulation of C6-methylvincosides successfully resulted in the discovered antimalarial framework (IC50 = 0.92 µM).

Efficacy of air purifier therapy for patients with allergic asthma

Objectives: This study aimed to evaluate the efficacy of air purifier therapy for patients with allergic asthma. Methods: Thirty-eight subjects were categorized under two groups namely treatment group and control group. All subjects were under 18 years of age and they had been clinically diagnosed with allergic asthma. The treatment group used high efficiency particulate air (HEPA) purifiers for six consecutive months, and the control group did not use the air filters. Particulate matter (PM) data and dust samples (from bedding and a static point) were collected from the subjects’ bedrooms before they started using the air purifiers and each month thereafter. Simultaneously, the subjects were asked to complete a questionnaire for the Asthma Control Test (ACT) or Childhood Asthma Control Test (C-ACT). Fractional exhaled nitric oxide (FENO) tests were performed at the start and end of the study. The concentrations of Der p1 and Der f1 were measured in the dust samples. Results: (1) After utilizing the air purifier, the concentrations of house dust mite (HDM) allergens (Der p1+ Der f1) in the dust samples decreased. In addition, the PMindoor/outdoor values significantly decreased. (2) The ACT and C-ACT scores in the treatment group maintained a steady significant upward trend. (3) At the end of the study, the FENO levels in both groups were lower, although the differences were not significant. Conclusions: It is witnessed that HEPA air purifiers can decrease indoor HDM allergen and PM levels and improve the quality of life for allergic asthma patients (AU)

[Preparation of Ag3PO4/g-C3N4 Composite Photocatalysts and Their Visible Light Photocatalytic Performance].

The visible light-driven photocatalyst Ag3PO4/g-C3N4 was synthesized by a simple in-situ precipitation method. The synthesized samples were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and UV-vis diffuse reflectance spectroscopy. Compared with that of single Ag3PO4 and g-C3N4, the Ag3PO4/g-C3N4 composite had a higher catalytic efficiency for levofloxacin. According to the energy band analysis and free radical capture experiment, the mechanism of the Z-type heterostructure of the Ag3PO4/g-C3N4 composite was proposed.

Discovery of 3-Cinnamamido-N-Substituted Benzamides as Potential Antimalarial Agents.

BACKGROUND: Malaria is one of the most devastating parasitic diseases, yet the discovery of antimalarial agents remains profoundly challenging. Very few new antimalarials have been developed in the past 50 years, while the emergence of drug-resistance continues to appear. OBJECTIVE: This study focuses on the discovery, design, synthesis, and antimalarial evaluation of 3- cinnamamido-N-substituted benzamides. METHODS: In this study, a screening of our compound library was carried out against the multidrugsensitive Plasmodium falciparum 3D7 strain. Derivatives of the hit were designed, synthesized and tested against P. falciparum 3D7 and the in vivo antimalarial activity of the most active compounds was evaluated using the method of Peters' 4-day suppressive test. RESULTS: The retrieved hit compound 1 containing a 3-cinnamamido-N-substituted benzamide skeleton showed moderate antimalarial activity (IC50 = 1.20 µM) for the first time. A series of derivatives were then synthesized through a simple four-step workflow, and half of them exhibited slightly better antimalarial effect than the precursor 1 during the subsequent in vitro assays. Additionally, compounds 11, 23, 30 and 31 displayed potent activity with IC50 values of approximately 0.1 µM, and weak cytotoxicity against mammalian cells. However, in vivo antimalarial activity is not effective, which might be ascribed to the poor solubility of these compounds. CONCLUSION: In this study, the phenotypic screen of our compound library resulted in the first report of a 3-cinnamamide framework with antimalarial activity and 40 derivatives were then designed and synthesized. Subsequent structure-activity studies showed that compounds 11, 23, 30 and 31 exhibited the most potent and selective activity against the P. falciparum 3D7 strain with IC50 values around 0.1 µM. Our work herein sets another example of phenotypic screen-based drug discovery, leading to potentially promising candidates of novel antimalarial agents once given further optimization.

Selective construction of alkaloid scaffolds by alcohol-based direct and mild aerobic oxidative Pictet-Spengler reactions.

Employing TBN/TEMPO as the catalysts and oxygen as the oxidant, the biologically and pharmaceutically significant tetrahydro-ß-carboline and ß-carboline alkaloid scaffolds that used to be obtained by multi-step processes can now be selectively obtained in only one-step via direct aerobic oxidative Pictet-Spengler reactions of tryptamines with alcohols under mild conditions, with water generated as the byproduct. In this reaction, TBN/TEMPO was interestingly found to be able to facilitate the cyclization step of the whole reaction. This method tolerates a variety of C- and N-substituted tryptamines, and both the more reactive benzylic and allylic alcohols and the less reactive aliphatic alcohols. This method can also be extended to dihydro-ß-carboline synthesis and applied to the more available and more economical tryptophan for ß-carboline synthesis, revealing its broad substrate scope and potential in synthetic applications.

DMSO-Triggered Complete Oxygen Transfer Leading to Accelerated Aqueous Hydrolysis of Organohalides under Mild Conditions.

Addition of DMSO is found to greatly accelerate the aqueous hydrolysis of organohalides to alcohols, providing a neutral, more efficient, milder and more economic process. Mechanistic studies using 18 O-DMSO and 18 O-H2 O showed that, contrary to the opinion that DMSO works as a dipolar solvent to enhance water's nucleophilicity, the accelerating effect comes from a complete oxygen transfer from DMSO to organohalides through generation of ROS+ Me2 ⋅X- salts through C-O bond formation, followed by O-S bond disassociative hydrolysis of ROS+ Me2 ⋅X- with water. This method is applicable to a wide range of organohalides and thus may have potential for practical industrial application, owing to easy recovery of DMSO from the H2 O/DMSO mixture by regular vacuum rectification.

Preoperative and postoperative medical therapies for chronic rhinosinusitis: National surveys among Chinese otolaryngologists.

OBJECTIVE: The aim of this study was to evaluate clinical practice patterns of preoperative and postoperative medical therapies immediately surrounding sinus surgery for chronic rhinosinusitis (CRS) by Chinese otolaryngologists. METHODS: Two anonymous web-based surveys of preoperative and postoperative medical therapies were performed. These surveys assessed the frequency of prescription of oral corticosteroids, intranasal corticosteroid sprays, oral antibiotics, nasal saline irrigation, oral antihistamines, nasal antihistamines, anti-leukotriene agents, topical decongestants and oral mucolytics. RESULTS: A total of 304 (17.5%) preoperative and 143 (23.5%) postoperative questionnaires were completed and returned. Seventy-eight percent, 63% and 56% of respondents used preoperative intranasal corticosteroid sprays, oral antibiotics and oral mucolytics "always or often", respectively. Ninety-four percent, 93%, 72% and 69% of respondents used postoperative intranasal corticosteroid sprays, nasal saline irrigation, oral antibiotics and oral mucolytics "always or often", respectively. Oral antihistamines, nasal antihistamines, anti-leukotrienes and topical decongestants were not commonly used preoperatively or postoperatively. CONCLUSIONS: Our study demonstrated that current practice patterns of preoperative medical therapies among otolaryngologists are not uniformly based on evidence-based outcomes research. Postoperative oral antibiotics, intranasal corticosteroid sprays, nasal saline irrigation and oral mucolytics are commonly used by a majority of Chinese otolaryngologist for CRS. Practice patterns of postoperative medical therapy reflect recent guidelines.

[Removal of Humic Acid from Water by Magnetic Chitosan-Grafted Polyacrylamide].

For high-efficiency removal of humic acid (HA), a natural organic matter in the water source, an adsorbent named magnetically modified chitosan-grafted polyacrylamide (MC-g-PAM) was developed by using an in situ coprecipitation method. Analytical instruments, such as a Fourier transform infrared (FTIR) spectroscope, scanning electron microscope (SEM), vibrating sample magnetometer (VSM), and specific surface area tester (BET), were used to characterize and analyze this material. With the aid of batch tests, the removal efficiency and mechanism of humic acid in water samples were investigated. The results show that the specific surface area and specific saturation magnetization values of the prepared MC-g-PAM are 27.065 m2·g-1 and 9.63 emu·g-1, respectively. The adsorption of humic acid by MC-g-PAM is an endothermic process and the Langmuir isotherm model and the pseudo-second-order kinetic equation fit the adsorption process well. At 25℃, the equilibrium adsorption capacity of MC-g-PAM to humic acid reaches 120.77 mg·g-1.

Synthesis and antitumor activity of novel 4-(2-fluorophenoxy)quinoline derivatives bearing the 4-oxo-1,4-dihydroquinoline-3-carboxamide moiety.

A series of 4-(2-fluorophenoxy)quinoline derivatives bearing the 4-oxo-1,4-dihydroquinoline-3-carboxamide moiety were designed, synthesized, and evaluated for their in vitro antitumor activity against the H460, HT-29, MKN-45, U87MG, and SMMC-7721 cancer cell lines. Most of the tested compounds showed potent activity and high selectivity toward the HT-29 and MKN-45 cell lines. Furthermore, compounds 21b, 21c, and 21i were further examined for their c-Met kinase activity and exhibited strong efficacy with IC50 values in the single-digit nanomolar range, which was comparable with the positive control foretinib. The most promising compound 21c showed excellent cytostatic activity with IC50 values from 0.01 to 0.53 µM against all tested cell lines, thus being 1.7-2.2 times more active than foretinib.

The orthographic sensitivity to written Chinese in the occipital-temporal cortex.

Previous studies have identified an area in the left lateral fusiform cortex that is highly responsive to written words and has been named the visual word form area (VWFA). However, there is disagreement on the specific functional role of this area in word recognition. Chinese characters, which are dramatically different from Roman alphabets in the visual form and in the form to phonological mapping, provide a unique opportunity to investigate the properties of the VWFA. Specifically, to clarify the orthographic sensitivity in the mid-fusiform cortex, we compared fMRI response amplitudes (Exp. 1) as well as the spatial patterns of response across multiple voxels (Exp. 2) between Chinese characters and stimuli derived from Chinese characters with different orthographic properties. The fMRI response amplitude results suggest the existence of orthographic sensitivity in the VWFA. The results from multi-voxel pattern analysis indicate that spatial distribution of the responses across voxels in the occipitotemporal cortex contained discriminative information between the different types of character-related stimuli. These results together suggest that the orthographic rules are likely represented in a distributed neural network with the VWFA containing the most specific information regarding a stimulus' orthographic regularity.