RESUMO
BACKGROUND: Gastric cancer (GC) develops from the lining of the stomach. The present study aimed to explore the effects of long non-coding RNA-ENST00000434223 (lncRNA ENST00000434223) on gastric cancer (GC) cells. METHODS: One hundred and four GC tissues and paracancerous tissues were collected from GC patients, and expression of ENST00000434223, Wnt2b, ß-catenin, cyclinD1, E-cadherin, N-cadherin, vimentin, and snail was subsequently assessed. Morphological changes in cells were assessed using an inverted microscope, and expression of Bcl-2, Bax and caspase-3 was examined. RESULTS: We found that expression of Wnt2b, ß-catenin, cyclinD1, N-cadherin, vimentin, and snail was increased in GC tissues, while expression of ENST00000434223 and E-cadherin was decreased. SGC-7901 cells were closely arranged, and expression of Wnt2b, ß-catenin, CyclinD1, N-cadherin, Vimentin, snail and Bcl-2 was increased, whereas expression of ENST00000434223, E-cadherin, Bax and caspase-3 was decreased. Furthermore, the rate of apoptosis was decreased and cell proliferation, invasion and migration were increased in response to downregulation of ENST00000434223. By contrast, upregulation of ENST00000434223 exhibited the opposite effects in MKN-45 cells. CONCLUSION: The results of this study provide a promising experimental basis for the treatment of gastric cancer through interventional targeting of lncRNA ENST00000434223.
Assuntos
Proliferação de Células/genética , Transição Epitelial-Mesenquimal/genética , RNA Longo não Codificante/genética , Neoplasias Gástricas/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Apoptose/genética , Caderinas/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Ciclina D1/genética , Progressão da Doença , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Glicoproteínas/genética , Humanos , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica/genética , Invasividade Neoplásica/patologia , Neoplasias Gástricas/patologia , Proteínas Wnt/genética , beta Catenina/genéticaRESUMO
Breast cancer is the most prevalent cancer and the leading cause of cancerassociated mortalities among women worldwide today. Accumulating evidence suggested that miR372 may serve important roles in the initiation and development of various human cancers. However, the role of miR372 in breast cancer remains unknown. The present study demonstrated that the expression level of miR372 in human breast cancer tissues and cell lines is significantly reduced compared with normal breast tissues cell lines. Furthermore, results of functional assays indicated that miR372 inhibits cell proliferation and induces apoptosis in the MCF7 human breast cancer cell line. E2F1 was identified as a direct functional target of miR372 in breast cancer. In conclusion, the findings revealed that miR372 may have the potential to act as a novel molecule for the diagnosis and therapy of patients with breast cancer.
