Phycosphere bacterial disturbance of Saccharina japonica caused by white rot disease relates to seawater nutrients.

In mid-November 2021, there were large areas of white rot disease on cultivated Saccharina japonica in Rongcheng City, China, and diseases were undetected on Sargassum horneri and Porphyra yezoensis. The disturbance direction of bacterial community in the phycosphere after disease outbreak and the relationship with seawater nutrients remain unclear. Here, in situ studies of bacterial community in the non-diseased and diseased areas (Shawo and Dongchu islands) and seawater nutrient levels were carried out. 16S rRNA sequencing showed that the bacterial richness of the studied seaweeds increased in the diseased area. Only in S. japonica, Algitalea outcompeted abundant primary bacteria with probiotic relationships to the host of the non-diseased area, and dominated in the diseased area (17.6% of the total abundance). Nitrogen and phosphorus levels in seawater were 57.8% and 19.6% higher in the non-diseased area than those in the diseased area, respectively, and were strongly correlated with the phycosphere bacteria at the family level of S. japonica. There was no difference in potential pathogenicity between the two areas, while positive signal communications decreased, and nitrogen cycle, chemoheterotrophy, and cellulolysis increased in the diseased area compared to the non-diseased area. Overall, white rot disease caused a structural disturbance in phycosphere bacterial community of S. japonica that related to seawater nutrient levels. Enriched degraders and altered bacterial community functions may exacerbate the disease. This evaluation will provide information for white rot disease management to prevent and mitigate the occurrence of S. japonica outbreaks.

The EH domain-containing protein, EdeA, is involved in endocytosis, cell wall integrity, and pathogenicity in Aspergillus fumigatus.

Endocytosis has been extensively studied in yeasts, where it plays crucial roles in growth, signaling regulation, and cell-surface receptor internalization. However, the biological functions of endocytosis in pathogenic filamentous fungi remain largely unexplored. In this study, we aimed to functionally characterize the roles of EdeA, an ortholog of the Saccharomyces cerevisiae endocytic protein Ede1, in Aspergillus fumigatus. EdeA was observed to be distributed as patches on the plasma membrane and concentrated in the subapical collar of hyphae, a localization characteristic of endocytic proteins. Loss of edeA caused defective hyphal polarity, reduced conidial production, and fewer sites of endocytosis initiations than that of the parental wild type. Notably, the edeA null mutant exhibited increased sensitivity to cell wall-disrupting agents, indicating a role for EdeA in maintaining cell wall integrity in A. fumigatus. This observation was further supported by the evidence showing that the thickness of the cell wall in the ΔedeA mutant increased, accompanied by abnormal activation of MpkA, a key component in the cell wall integrity pathway. Additionally, the ΔedeA mutant displayed increased pathogenicity in the Galleria mellonella wax moth infection model, possibly due to alterations in cell wall morphology. Site-directed mutagenesis identified the conserved residue E348 within the third EH (Eps15 homology) domain of EdeA as crucial for its subcellular localization and functions. In conclusion, our results highlight the involvement of EdeA in endocytosis, hyphal polarity, cell wall integrity, and pathogenicity in A. fumigatus. IMPORTANCE: Aspergillus fumigatus is a significant human pathogenic fungus known to cause invasive aspergillosis, a disease with a high mortality rate. Understanding the basic principles of A. fumigatus pathogenicity is crucial for developing effective strategies against this pathogen. Previous research has underscored the importance of endocytosis in the infection capacity of pathogenic yeasts; however, its biological function in pathogenic mold remains largely unexplored. Our characterization of EdeA in A. fumigatus sheds light on the role of endocytosis in the development, stress response, and pathogenicity of pathogenic molds. These findings suggest that the components of the endocytosis process may serve as potential targets for antifungal therapy.

High-frequency rTMS of the left dorsolateral prefrontal cortex for post-stroke depression: A systematic review and meta-analysis.

OBJECTIVE: This meta-analysis investigated the therapeutic efficacy of high-frequency repetitive transcranial magnetic stimulation (rTMS) over the left dorsolateral prefrontal cortex (DLPFC) for treatment of post-stroke depression (PSD). METHODS: Ten articles with 266 patients in rTMS group and 258 patients in control group were included. The primary outcome was performed to examine the efficacy of rTMS for PSD. Secondary outcomes of response rates and remission rates and subgroup analyses were further explored. RESULTS: Our meta-analysis revealed a significant pooled effect size (the standard mean difference (SMD) was -1.45 points (95% CI, -2.04 to -0.86; p < 0.00001)). The odds ratio (OR) of the response rate and remission rate were 8.41 (95% CI, 2.52-28.12, p = 0.0005) and 6.04 (95% CI, 1.5-24.39, p = 0.01). Moreover, rTMS treatment for PSD patients in subacute phase and targeting the left DLPFC at 5-cm anterior to the left motor hotspot or the midpoint of the middle frontal gyrus showed significant antidepressant effect. In addition, the Hamilton Depression Rating Scale (HAMD) was sensitive to detect depressive changes in patients. CONCLUSIONS: Our meta-analysis elucidated that the application of high-frequency rTMS over the left DLPFC was an effective treatment alternative for PSD. SIGNIFICANCE: Our meta-analysis may help to develop more reasonable treatment strategies in clinical practice for PSD patients.

Safety profile of intravenous digoxin in Chinese patients with acute heart failure with reduced ejection fraction: a small-scale prospective cohort study.

Background: Adverse effects of intravenous digoxin vary from patients and disease status, which should be closely monitored. Aims: To explore the safety profile of intravenous digoxin in acute heart failure with reduced ejection fraction (HFrEF) among Chinese patients. Methods: A clinical prospective, single-center, single-arm, open-label exploratory clinical trial was performed in patients with acute HFrEF at Wuhan Asia Heart Hospital. A fixed dose of 0.5 mg digoxin was used intravenously once per day for 3 days. The normalized dosage of digoxin (NDD), toxic serum digoxin concentration (SDC), and adverse reactions of intravenous digoxin were recorded. Results: A total of 40 patients were recruited in the study. The SDC increased from 1.03 ± 0.34 ng/mL to 1.95 ± 0.52 ng/mL during treatment. 50% (20/40) patients reached a toxic SDC of 2.0 ng/mL, and toxic effects were seen in 30% (12/40) patients. Estimated glomerular filtration rate (eGFR) < 60 mL/min [HR: 5.269; 95% CI: 1.905-14.575, p = 0.001], NDD ≥7 µg/kg [HR: 3.028; 95% CI: 1.119-8.194, p = 0.029], and ischemic cardiomyopathy [HR: 2.658; 95% CI: 1.025-6.894, p = 0.044] were independent risk factors for toxic SDC. Toxic SDC was effectively identified [area under the receiver operating characteristic (ROC) curve = 0.85, p < 0.001] using this model, and patients would have a higher risk of toxicity with more risk factors. Conclusion: Intravenous digoxin of 0.5 mg was safe and effective for initial dose but not suitable for maintenance treatment in Chinese patients with acute HFrEF. Patients who had lower eGFR, received higher NDD, and had ischemic cardiomyopathy should be closely monitored to avoid digoxin toxicity.

Phase stability diagram, self-similar structures, and multistability in a free-running VCSEL with a small misalignment between the phase and amplitude anisotropies.

We report on the global dynamics of a free-running vertical-cavity surface-emitting laser (VCSEL) with misalignment between the linear phase and amplitude anisotropies due to the fact that this case might occur in practice caused unintentionally by minor manufacturing variations or design, in virtue of high-resolution phase stability diagrams, where two kinds of self-similar structures are revealed. Of interest is that the Arnold tongue cascades covered by multiple distinct periodicities are discovered for the first time in several scenarios specified in the free-running VCSEL, to the best of our knowledge. Additionally, we also uncover the existence of multistability through the basin of the attraction, as well as the eyes of anti-chaos and periodicity characterized by fractal. The findings may shed new light on interesting polarization dynamics of VCSELs, and also open the possibility to detect the above-mentioned structures experimentally and develop some potential applications.

Extreme events in two laterally-coupled semiconductor lasers.

Rogue waves (RWs) are extreme and rare waves that emerge unexpectedly in many natural systems and their formation mechanism and prediction have been extensively studied. Here, we numerically demonstrate the appearance of extreme events (EEs) for the first time, to the best of our knowledge, in the chaotic regimes of a two-element coupled semiconductor laser array. Based on coupled-mode theory, we characterize the occurrence of EEs by calculating the probability distribution, which confirms the RW-type feature of the intensity pulses, i.e., non-Gaussian distribution. Combining with the results of the 0-1 test for chaos, we confirm that EEs originate from deterministic nonlinearities in coupled semiconductor laser systems. We show that EEs can be predicted with a long anticipation time. Furthermore, simulation results manifest that the occurrence probability of EEs can be flexibly tuned by tailoring the coupling parameter space. With the help of two-dimension maps, the effects of key parameters, i.e., the waveguide structure and the pump level, on the formation of EEs are discussed systematically. This work provides a new platform for the research of EEs in a highly integrated structure and opens up a novel investigation field for coupled semiconductor laser arrays.

Fatigue life prediction of brake discs for high-speed trains via thermal stress.

As one of the essential components of the braking system, the high-speed train brake disc is an integral part of ensuring the safety of the train. The main objective of this study is to conduct thermal analysis and reliable life prediction on brake discs. The research was conducted by developing a programmer spectrum of brake discs with high-speed train brake discs. According to the results of numerical analysis and the S-N curve of the material, the temperature distribution on the surface of the brake disc is determined, and the fatigue life of the brake disc is predicted. The comparison with the service life of the brake disc verifies the rationality of the calculation results. In addition, a fatigue damage probability model of brake discs is established based on the theory of fatigue cumulative damage. Through the relationship between the reliability of mechanical parts and the number of load cycles, the reliable life of the brake disc under different working conditions is predicted. This work establishes a method of reliable life analysis for brake discs of high-speed trains based on the load spectrum, which could analyze the life and reliability of brake discs more systematically.

Process optimization for fermented siwu decoction by multi-index-response surface method and exploration of the effects of fermented siwu decoction on the growth, immune response and resistance to Vibrio harveyi of Pacific white shrimp (Litopenaeus vannamei).

The purpose of this study was to explore the optimal fermentation technology of Chinese herbal medicine formula-Siwu Decoction and the effects of fermented Siwu Decoction (FSW) on the growth performance, immune response, intestinal microflora and anti microbial ability of Litopenaeus vannamei. Response to surface methodology (RSM) was used to optimize the fermentation process of Siwu Decoction. The optimal fermentation conditions were obtained as follows: inoculation amount of mixed strains was 4.5%, fermentation time was 36 h, and the ratio of material to liquid was 20%. A total of 1260 shrimps were selected and divided into seven groups, three in parallel in each group. The dietary level of each group was as follows: Control （No additions）, USW1 (0.2% unfermented herbal medicine), USW2 (0.5% unfermented herbal medicine), USW3 (0.8% unfermented herbal medicine), FSW1 (0.2% fermented herbal medicine), FSW2 (0.5% fermented herbal medicine), FSW3 (0.8% fermented herbal medicine). The immune response and antioxidant defense ability of hemocytes and intestine were measured at 21 and 42 days of feeding and the intestinal flora and growth performance were measured at 42 days of feeding, after that, a 7-day challenge test against Vibrio harveyi was conducted. The results showed that fermented Siwu Decoction significantly improved the growth performance and body composition of Litopenaeus vannamei; significantly increased the total number of hemocytes, phagocytic activity, antibacterial activity and bacteriolytic activity of Litopenaeus vannamei, and improved the antioxidant activity of Litopenaeus vannamei; the addition of fermented Siwu Decoction significantly increased the gene expression level of hemocytes and intestinal tract of Litopenaeus vannamei, and improved the antioxidant activity of Litopenaeus vannamei. The abundance of Bacillus increased, while the abundance of Vibrio decreased. After Vibrio harveyi challenge, the cumulative mortality of FSW group was significantly lower than that of control group. Fermented Siwu Decoction may be a potential physiological enhancer in aquaculture, and can be widely used in aquaculture.

Electron-Ion Coupling Mechanism to Construct Stable Output Performance Nanogenerator.

Recently, triboelectric nanogenerators (TENGs) have been promoted as an effective technique for ambient energy harvesting, given their large power density and high energy conversion efficiency. However, traditional TENGs based on the combination of triboelectrification effect and electrostatic induction have proven susceptible to environmental influence, which intensively restricts their application range. Herein, a new coupling mechanism based on electrostatic induction and ion conduction is proposed to construct flexible stable output performance TENGs (SOP-TENGs). The calcium chloride doped-cellulose nanofibril (CaCl2-CNF) film made of natural carrots was successfully introduced to realize this coupling, resulting from its intrinsic properties as natural nanofibril hydrogel serving as both triboelectric layer and electrode. The coupling of two conductive mechanisms of SOP-TENG was comprehensively investigated through electrical measurements, including the effects of moisture content, relative humidity, and electrode size. In contrast to the conventional hydrogel ionotronic TENGs that require moisture as the carrier for ion transfer and use a hydrogel layer as the electrode, the use of a CaCl2-CNF film (i.e., ion-doped natural hydrogel layer) as a friction layer in the proposed SOP-TENG effectively realizes a superstable electrical output under varying moisture contents and relative humidity due to the compound transfer mechanism of ions and electrons. This new working principle based on the coupling of electrostatic induction and ion conduction opens a wider range of applications for the hydrogel ionotronic TENGs, as the superstable electrical output enables them to be more widely applied in various complex environments to supply energy for low-power electronic devices.

Prediction and evaluation of health state for power battery based on Ridge linear regression model.

The state of health (SOH) of power battery reflects the difference between the current performance of the battery and the time it left the factory. Accurate prediction of it is the key to improving battery cycle efficiency. This paper studies the application of data-driven algorithms in power battery health estimation. Firstly, Using the data of actual operating vehicles which are monitoring in the data platform as the research objects. The charging event segmentation algorithm is designed for the full amount of data, and the K-means clustering model is used to extract slow charging events. Secondly, feature engineering is performed on the data, including the use of Pearson and Spearman coefficients analysis for numerical features, the use of one-hot encoding for category features to determine the final input features of SOH model. Eventually, using the Ridge linear regression model to predict the health status of the power battery. The research shows that the MAE is less than 5%, which meets the needs of practical use. In addition, this paper comparing Ridge with three other models named Linear Regression, Lasso, and Elastic Net. The result showed that the linear regression model with L2 regularization is more applicable in low-dimensional feature application scenarios without cell data in prediction of SOH.

Wedelolactone ameliorates Pseudomonas aeruginosa-induced inflammation and corneal injury by suppressing caspase-4/5/11/GSDMD-mediated non-canonical pyroptosis.

Pseudomonas aeruginosa (P. aeruginosa) keratitis, a worldwide leading cause of corneal perforation and blindness, which is associated with contact lens usage. Increasing evidence has indicated that pyroptosis, a novel proinflammatory programmed cell death, is linked with ocular diseases, little is known about the role of noncanonical pyroptosis in microbial keratitis. Here, we first indicated the involvement of noncanonical pyroptosis in P. aeruginosa keratitis and investigated whether wedelolactone (WDL), a major active component of Eclipta prostrate known to target caspase-11, could alleviate P. aeruginosa keratitis development. We found the expression of caspase-4/5/11 and cleaved GSDMD in corneas of P. aeruginosa keratitis patients, animal models and lipopolysaccharide (LPS)-induced primary cultured human corneal keratocytes (piHCKs) were increased. Combining ciprofloxacin with WDL significantly ameliorated the severity of P. aeruginosa keratitis, as manifested by decreased inflammatory responses and reduced corneal epithelial defects. Consistent with these findings, WDL also dose-dependently alleviated LPS-induced noncanonical pyroptosis by reversing the increased expression of caspase-4/5 and GSDMD in piHCKs. In summary, our results demonstrated that by targeting the activation of caspase-4/5/11, wedelolactone inhibited the development of P. aeruginosa keratitis and suppressed the release of proinflammatory cytokines. Wedelolactone may be a promising anti-inflammatory candidate to combat P. aeruginosa keratitis.

Transcriptome profiling based on protein-protein interaction networks provides a set of core genes for understanding the immune response mechanisms of the egg-protecting behavior in Octopus ocellatus.

Protection via of the immune system is indispensable to the life of organisms. Within an immune network, problems with a given link will affect the normal life activities of the organism. Octopus ocellatus is cephalopod widely distributed throughout the world's oceans. Because of its unique nervous system and locomotive organs, research on this species has gradually increased in recent years. Many immune response mechanisms associated with behaviors of O. ocellatus are still unclear. Moreover, as a factor affecting the normal growth of O. ocellatus, egg protection has rarely been considered in previous behavioral studies. In this study, we analyzed the transcriptome profile of gene expression in O. ocellatus larvae, and identified 5936 differentially expressed genes (DEGs). GO and KEGG enrichment analyses were used to search for immune-related DEGs. Protein-protein interaction networks were constructed to examine the interactions between immune-related genes. Fifteen hub genes involved in multiple KEGG signaling pathways or with multiple protein-protein interaction relationships were obtained and verified by quantitative RT-PCR. We first studied the effects of egg protection on the immunity of O. ocellatus larvae by means of protein-protein interaction networks, and the results provide valuable genetic resources for understanding the immunity of invertebrate larvae. The data serve as a foundation for further research on the egg-protecting behavior of invertebrates.

Effect of dietary Xiao-Chaihu-Decoction on growth performance, immune response, detoxification and intestinal microbiota of pacific white shrimp (Litopenaeus vannamei).

Xiao-Chaihu-Decoction (XCHD), a classical traditional Chinese medicine with diverse biological activities, is widely applied to prevent and treat many human diseases. Effects of dietary XCHD on growth performance, immune response, detoxification system, intestinal microbiota and resistance against aflatoxin B1(AFB1) of Litopenaeus vannamei was studied. Four isonitrogenous and isolipidic diets were formulated to contain 0, 1, 2, and 5 g/kg (control, XCHD1, XCHD2 and XCHD3) of XCHD, respectively. Seven hundred and eighty shrimp (1.16 ± 0.09 g) were assigned randomly to 12 tanks (400 L, three tanks each group, 65 shrimp in each tank) for 6 weeks. After sampling, 25 shrimp from each tank were selected for a 2-week AFB1 (2500 µg/kg) challenge experiment. The results indicated that the final weight, weight gain and specific growth rate in XCHD2 and XCHD3 groups were significantly increased compared to control. The protease, amylase, superoxide dismutase (SOD), glutathione s-transferase (GST), sulfotransferase (SULT) activities, total antioxidant capacity (T-AOC) and glutathione (GSH) contents in hepatopancreas were significantly increased in XCHD3 groups and the expressions of immune-related genes (Toll, Dorsal and Cru) in hepatopancreas were significantly up-regulated in XCHD2 and XCHD3 groups. High-throughput sequencing analysis revealed that the abundance of Proteobacteria decreased and the abundances of Bacteroidetes increased in XCHD2 and XCHD3 groups. Additionally, AFB1 challenge experiments showed that AFB1 caused histological damage to the hepatopancreas and significantly increased the levels of malondialdehyde (MDA) and protein carbonylation (PC) in hepatopancreas as well as the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Nevertheless, XCHD could effectively alleviated the growth toxicity, immunosuppression and macromolecular damage caused by AFB1 to shrimp by inhibiting the Phase I enzyme and enhancing Phase II enzyme and antioxidant system.

Bilateral Limbal Stem Cell Alterations in Patients With Unilateral Herpes Simplex Keratitis and Herpes Zoster Ophthalmicus as Shown by In Vivo Confocal Microscopy.

Purpose: The purpose of this study was to investigate the limbal changes in the palisades of Vogt (POV) in patients with herpes simplex keratitis (HSK) and herpes zoster ophthalmicus (HZO) with the application of in vivo confocal microscopy (IVCM). Methods: We enrolled 35 eyes of 35 consecutive patients with HSK and 4 patients with HZO in this observational study. Thirty-five participants were also recruited from a healthy population as the control group. All subjects were examined by IVCM in addition to routine slit-lamp biomicroscopy. The IVCM images of the corneal basal epithelial cells, corneal nerve, and the corneoscleral limbus were acquired and then were analyzed semiquantitatively. Results: The rate of absent and atypical POV was significantly higher in the affected eyes of patients with HSK than in the contralateral eyes and eyes of controls (88.57% vs. 65.71% vs. 17.14%, P < 0.01). In the HZO group, the rate of absent and atypical POV was 100% in the affected eyes and 50% in the contralateral eyes. When compared to the contralateral unaffected eyes and control eyes, the average density of the central basal epithelial cells and the sub-basal nerve plexus density and the total number of nerves in the central area of the affected eyes were significantly lower in the HSK group (1541 ± 704.4 vs. 2510 ± 746.8 vs. 3650 ± 746.1 cells/mm2, P < 0.0001). Spearman's rank correlation showed that the presence of absent and atypical POV had a significant negative correlation with central corneal basal epithelial cells (rs = -0.44979, P < 0.0001), the density of total nerves (rs = -0.49742, P < 0.0001), and the total nerve numbers (rs = -0.48437, P < 0.0001). A significant positive correlation was established between the presence of absent and atypical POV and HSK severity in affected eyes in the superior, inferior, nasal, and temporal quadrants (rs = 0.68940, rs = 0.78715, rs = 0.65591, and rs = 0.75481, respectively, P < 0.0001) and the contralateral eyes (rs = 0.51636, rs = 0.36207, rs = 0.36990, rs = 0.51241, correspondingly, P < 0.0001). Conclusions: Both eyes of patients with unilateral HSK and HZO demonstrated a profound and significant loss of limbal stem cells, which may explain the fact that HSK and HZO are risk factors for limbal stem cell deficiency (LSCD) in both eyes. The loss of LSCs was strongly correlated with the sub-basal nerve plexus and central basal epithelial cell alterations as shown by IVCM.

Dual Active Center-Assembled Cu31S16-Co9-xNixS8 Heterodimers: Coherent Interface Engineering Induces Multihole Accumulation for Light-Enhanced Electrocatalytic Oxygen Evolution.

The design of low-cost yet highly efficient electrocatalysts plays a critical role in energy storage and conversion reactions. The oxygen evolution reaction (OER) is considered a bottleneck of electrochemical water splitting for hydrogen fuel generation. It is still challenging to extract a high density of charge carriers in noble-metal-free alternative catalysts to facilitate sluggish kinetics. Herein, we report the rational design and coherent interface engineering for combining light-harvesting Cu31S16 with electroactive Co9-xNixS8 (x = 0-9) to form novel Cu31S16-Co9-xNixS8 heterodimers. By delicately controlling the kinetic growth in a seed-mediated growth method, the bifunctional centers, even with two distinct crystal phases, were integrated into a synergistic architecture, which achieved full-spectrum solar energy capture and light conversion to drive and activate the electrochemical reaction. Benefiting from the well-defined structure, high-quality interface, oriented attachment, and optimal Co/Ni bimetal ratio, Cu31S16-Co7.2Ni1.8S8 produces a dramatically reduced overpotential (242 mV at 10 mA cm-2) with a shift of 83 mV under visible-light excitation, achieving a 4.5-fold higher turnover frequency than that of its unirradiated Co7.2Ni1.8S8 counterpart. This enhanced performance also far exceeds commercial RuO2 (358 mV at 10 mA cm-2) and most nonprecious-metal nanocatalysts. Further mechanistic studies reveal that coherent interface engineering leads to a strong photo/electricity coupling effect and efficient spatial charge separation, which induces sufficient hot holes that eventually accumulate at the electroactive sites to accelerate the multihole-involved OER. This work would open up new opportunities for the fabrication of non-noble metal electrocatalysts and management of charge carriers.

The Interface between Nanoenergy and Self-Powered Electronics.

In recent decades, nanogenerators based on several techniques such as triboelectric effects, piezoelectric effects, or other mechanisms have experienced great developments. The nanoenergy generated by nanogenerators is supposed to be used to overcome the problem of energy supply problems for portable electronics and to be applied to self-powered microsystems including sensors, actuators, integrated circuits, power sources, and so on. Researchers made many attempts to achieve a good solution and have performed many explorations. Massive efforts have been devoted to developing self-powered electronics, such as self-powered communication devices, self-powered human-machine interfaces, and self-powered sensors. To take full advantage of nanoenergy, we need to review the existing applications, look for similarities and differences, and then explore the ways of achieving various self-powered systems with better performance. In this review, the methods of applying nanogenerators in specific circumstances are studied. The applications of nanogenerators are classified into two categories, direct utilization and indirect utilization, according to whether a treatment process is needed. We expect to offer a line of thought for future research on self-powered electronics.

Ocular pathogens and antibiotic resistance in microbial keratitis over three years in Harbin, Northeast China.

PURPOSE: Understanding the spectrum of ocular pathogens in a given geographic region is important for devising appropriate practical treatment. Therefore, we examined the pathogen spectrum and antibiotic resistance of microbial keratitis in northeast China. METHODS: In this retrospective observational study, we reviewed the microbiology laboratory records of patients diagnosed with microbial keratitis in a tertiary eye hospital in Harbin, northeast China, between 2017 and 2019, and analysed the pathogen spectrum and antibiotic susceptibility. RESULTS: We collected 462 specimens, of which 282 exhibited positive cultures. Among these cultures, 257 were bacterial and 25 were fungal. Of the 257 bacterial isolates, 214 (83.27%) were gram positive whereas 43 (16.73%) were gram negative. The most prevalent gram-positive pathogen was coagulase-negative staphylococcus (CoNS; 58.37%), followed by Staphylococcus aureus (S. aureus; 20.62%) and Streptococcus pneumoniae (2.33%). Of the gram-negative bacterial isolates, 10 were Pseudomonas aeruginosa (3.89%). The most frequently detected ocular pathogens from fungal isolates were Fusarium species (40%). We also found more culture-positive cases of bacterial keratitis in summer. Overall, 16.98% S. aureus and 64.00% CoNS isolates were methicillin resistant. These methicillin-resistant bacteria were also more likely to be resistant to other antibiotics, with multidrug resistance found in 77.78% methicillin-resistant S. aureus and 90.63% methicillin-resistant CoNS. However, all gram-positive isolates were sensitive to vancomycin and linezolid. CONCLUSION: Coagulase-negative staphylococcus are the most common ocular pathogens in northeast China. We also show the prevalence of methicillin resistance and concurrent multidrug resistance among staphylococcal isolates. Monitoring the patterns of antimicrobial resistance could help in the management selection.

Tolerance analysis for automobile transmission shaft based on minimum cost and reliability target.

To design a reasonable dimensional tolerance of a transmission shaft, the higher product quality while lower manufacturing cost must be considered. This paper provides a mathematical model and a flowchart which elucidates the relationship between process capability index (PCI), reliability, tolerance and manufacturing cost, considering the characteristics of the shaft diameter, the material and manufacturing process. A 10.904% cost reduction under certain PCI range of a real case shows the effectiveness of the model and flowchart, thus it can be applied to those technical area when optimizing product design.

Phase-field study of the effects of the multi-controlling parameters on columnar dendrite during directional solidification in hexagonal materials.

The growth of hexagonal columnar dendrite during directional solidification with respect to the multi-controlling parameters such as anisotropy, cooling rate, temperature gradient and orientation angle were investigated by a quantitative phase-field method, respectively. The simulation results show that the increase of anisotropy, cooling rate and temperature gradient can accelerate the solidification velocity of columnar dendrites. Among them, the cooling rate has the most significant effect on the solidification velocity of columnar dendrite. In contrast, the solidification velocity of columnar dendrite slows down with the increase of the orientation angle. Meanwhile, the primary dendrite spacing decreases with the increase of cooling rates and temperature gradient, and the primary dendrite arms are smooth. The primary dendrite spacing increases with the increase of anisotropy and orientation angle, which provides space for the development of secondary dendrite arms. In addition, the effects of cooling rate and temperature gradient on the solid volume fraction were also studied.

Serum IgM against SARS-CoV-2 correlates with in-hospital mortality in severe/critical patients with COVID-19 in Wuhan, China.

Severe/critical patients with coronavirus disease 2019 (COVID-19) have become the central issue in the current global pandemic due to their high mortality rate. However, the relationship between antibody response and clinical outcomes has not been well described in this group. We conducted a single-center, retrospective, cohort study to investigate the relationship between serum immunoglobulin G (IgG) and IgM and clinical outcomes in severe/critical patients with COVID-19. Seventy-nine severe/critical patients with COVID-19 admitted in Wuhan Asia General Hospital in Wuhan, China during January 22, 2020 to March 6, 2020 were included. Serum antibodies were measured at day 25 (SD, 7) post illness onset. The median IgG titer was 113 (IQR 81-167) AU/ml, and IgM titer was 50 (IQR, 23-105) AU/ml. Patients whose IgM titer ≥ 50 AU/ml had higher in-hospital mortality (p=0.026). IgM titer ≥ 50 AU/ml was also correlated with higher incidences of Acute Respiratory Distress Syndrome (ARDS) and sepsis shock. Antibody remeasurements were performed in 42 patients, where IgM titer declined significantly in survivors (p=0.031). Serum IgM titer changes according to the COVID-19 progression. The severe/critical patients with COVID-19 have a higher risk of clinical adverse events when IgM titer ≥ 50 AU/ml. Further decreasing of IgM could imply a better outcome in severe/critical cases.