Enhancing tomato fruit antioxidant potential through hydrogen nanobubble irrigation.

Eating fruits and vegetables loaded with natural antioxidants can boost human health considerably and help fight off diseases linked to oxidative stress. Hydrogen has unique antioxidant effects. However, its low-solubility and fast-diffusion has limited its applications in agriculture. Integration of hydrogen with nanobubble technology could address such problems. However, the physiological adaptation and response mechanism of crops to hydrogen nanobubbles is still poorly understood. Antioxidant concentrations of lycopene, ascorbic acid, flavonoids, and resveratrol in hydrogen nanobubble water drip-irrigated tomato fruits increased by 16.3-264.8% and 2.2-19.8%, respectively, compared to underground water and oxygen nanobubble water. Transcriptomic and metabolomic analyses were combined to investigate the regulatory mechanisms that differed from the controls. Comprehensive multi-omics analysis revealed differences in the abundances of genes responsible for hormonal control, hydrogenase genes, and necessary synthetic metabolites of antioxidants, which helped to clarify the observed improvements in antioxidants. This is the first case of hydrogen nanobubble water irrigation increasing numerous natural antioxidant parts in fruits. Considering the characteristics of hydrogen and the application of the nanobubble technology in agriculture, the findings of the present study could facilitate the understanding of the potential effects of hydrogen on biological processes and the mechanisms of action on plant growth and development.

Unlocking osmotic energy harvesting potential in challenging real-world hypersaline environments through vermiculite-based hetero-nanochannels.

Nanochannel membranes have demonstrated remarkable potential for osmotic energy harvesting; however, their efficiency in practical high-salinity systems is hindered by reduced ion selectivity. Here, we propose a dual-separation transport strategy by constructing a two-dimensional (2D) vermiculite (VMT)-based heterogeneous nanofluidic system via an eco-friendly and scalable method. The cations are initially separated and enriched in micropores of substrates during the transmembrane diffusion, followed by secondary precise sieving in ultra-thin VMT laminates with high ion flux. Resultantly, our nanofluidic system demonstrates efficient osmotic energy harvesting performance, especially in hypersaline environment. Notably, we achieve a maximum power density of 33.76 W m-2, a 6.2-fold improvement with a ten-fold increase in salinity gradient, surpassing state-of-the-art nanochannel membranes under challenging conditions. Additionally, we confirm practical hypersaline osmotic power generation using various natural salt-lake brines, achieving a power density of 25.9 W m-2. This work triggers the hopes for practical blue energy conversion using advanced nanoarchitecture.

Influence of double-layer filling structure on nitrogen removal and internal microbial distribution in bioretention cells.

The nitrogen removal effect of traditional bioretention cells on runoff rainwater is not stable. The nitrogen removal effect of bioretention cells can be improved by setting up a layered filling structure, but the effect of changes in filling structure on the nitrogen removal process and microbial community characteristics is still unclear. Two types of porosity fillers were set up in the experiment, and a homogeneous bioretention cell and three bioretention cells with layered fillers were constructed by changing the depth range of the upper and lower layers to analyze the influence of the pore variation of different depth fillers on the nitrogen removal process and microbial community characteristics. The experimental results showed that, compared with the homogeneous filing structure, the layered filling structure can strengthen the adsorption of NH4+-N and the conversion of NO3--N, so as to increase the removal rates of NH4+-N and NO3--N by 20.71-81.56% and 9.25%-78.19%, respectively. Although the low porosity filler structure will reduce the nitrification activity and urease activity by 48.63%-66.68% and 8.00%-20.64% respectively, it can increase the denitrification activity by 19.14%-31.92%, thus significantly reducing the nitrate content in the filler. The low porosity filler structure can affect the growth and reproduction of various phylum bacteria such as Proteobacteria, Chloroflexi, Acidobacteria, and genus bacteria such as Nitrospira, Ellin6067, Rhizobacter, Pseudomonas, which can improve the diversity and richness of microorganisms.

Nitrogen transfer and transformation in bioretention cells under low temperature conditions.

The nitrogen removal effect of traditional bioretention cells is generally poor under low temperature conditions, with significant levels of fluctuation and leaching often reported. Therefore, the migration characteristics of nitrogen were explored in bioretention cells under low temperature conditions, with the aim of improving the nitrogen removal effect. Four groups of modified collapsible loess bioretention cells were constructed and operated at 1, 5, 10 and 25 °C. The nitrogen removal effect of the cells was determined at different temperatures and the nitrogen migration and transformation characteristics under low temperature conditions were discussed. Experimental results showed that during the rainfall period, the ammonia nitrogen removal efficiency remained similar at different temperatures (above 97 %), while the nitrate nitrogen removal efficiency varied significantly at 1, 5, 10 and 25 °C, from 28.15 %-65.22 %, 96.68 %-98.8 %, 96.75 %-98.88 % and 80.14 %-96.72 %, respectively. In addition, nitrate nitrogen accumulation occurred in the filler during rainfall events, with lower temperature conditions increasing the final concentration of nitrate nitrogen accumulated. Following a rainfall event, the content of nitrate nitrogen in the filler decreased significantly over a 60 h dry period. However, the nitrate nitrogen reduction rate was significantly lower under low temperature conditions, than at 25 °C. Overall, low temperature conditions had a negative effect on the accumulation of nitrate nitrogen in the filler during rainfall events, as well as the transformation and migration of nitrate nitrogen within the filler during drought periods, with the adverse effects most significant at temperatures lower than 5 °C.

Effects of freeze-thaw cycles on nutrient removal from bioretention cells.

There have been numerous summaries of the runoff purification characteristics of bioretention cells in warm climates. However, little has been done on the effects of freeze-thaw cycles (FTCs) that frequently occur in cold regions on bioretention cell performance. Three experimental columns were constructed to simulate the operation of the bioretention cell under the FTCs. The effects of FTCs on the nutrient removal efficiency of different filling bioretention cells were analyzed. The results showed that the ammonia nitrogen (NH4+-N) concentration in the effluent of the wood chip bioretention cell under the T3 conditions (WBCF) (2.35 mg/L) was significantly higher than that of the wood chip bioretention cell operating at room temperature (WBCR) (0.62 mg/L). The effluent NH4+-N concentration of aluminum sludge bioretention cell (ABCF) (0.096 mg/L) under the FTCs was lower than that of WBCF (0.91 mg/L). Under the T3 condition, the effluent nitrate nitrogen (NO3--N) and total nitrogen (TN) concentrations of WBCF (5.33 mg/L and 8.86 mg/L) were higher than those of WBCR (5 mg/L and 6.11 mg/L) at room temperature. Under FTCs conditions, both WBCF and ABCF had high NO3--N removal efficiency (up to 85.87% and 24.75%) at the initial stage of thawing of the filler, and the efficiency gradually decreased with the thawing of the filler. With the increase of FTCs, the NO3--N removal efficiency of WBCF gradually decreased (always higher than 13.6%), while the removal efficiency of ABCF fluctuated wildly (the removal efficiency was primarily negative). The total phosphorus (TP) concentration in the effluent of WBCF (0.11 mg/L) under the T3 conditions was lower than that of WBCR (0.02 mg/L) at room temperature, and the TP concentration of ABCF (0.021 mg/L) in the effluent under the FTCs was slightly lower than that of WBCF (0.031 mg/L). The FTCs have a more significant impact on removing nitrogen pollutants in runoff, but have little effect on phosphorus. Compared with aluminum sludge, wood chips are more suitable for efficient removal of nitrogen pollutants in runoff under the FTCs. The experimental conclusions can provide a reference for the construction of bioretention cells in cold regions.

Religion, Psychological Strain, and Suicidality in China: A Preliminary Study.

BACKGROUND: There is a lack of religiosity studies in China especially in relation to mental health and suicidality. In this research, we focus our studies on medical workers of which some studies reported to have higher stress, and to pilot our studies in this adult age-group. DATA AND METHODOLOGY: Data were obtained by a questionnaire survey in a large public hospital in a big metropolitan city of China. The final sample consisted of 1012 respondents with 237 (23.4%) being male and 775 (76.6%) being female. The respondents were of three groups: (1) Believers (n = 34; 3.5%); (2) Non-Believers or Atheists (n = 547; 55.8%); and (3) Agnostics or Fence-Sitters (n = 400; 40.8%). Suicidality was measured by the NCS-Suicidality Scale, and standard measures were employed for other major variables. FINDINGS: In line with other recent studies in China, the religion rate among the urban adults remained low (3.5%). However, about 40.8% of the respondents chose "don't know" and could be fence-sitters on the issue of religious belief. Many of them are involved in various folk beliefs which may not be considered as religious. The religious believers were at higher risk of suicidality and depression than the atheists and the fence-sitters. However, the fence-sitters were higher than the believers and atheists on psychological strains, and they were higher on depression compared to the atheists. CONCLUSION: The religious believers and religious fence-sitters have higher psychopathologic risks and suicidal risk than the atheist group. Religion as of low prevalence in Chinese societies is a social value deviant from the norm and its practitioners are likely to be marginalized or stigmatized. The Strain Theory of Suicide is used for detailed explanations.

Selenium fertigation with nanobubbles influences soil selenium residual and plant performance by modulation of bacterial community.

Although selenium (Se) is an essential microelement for humans and animals, it is a potentially toxic element due to its bioaccumulation potential. In this study, Se fertilizer was supplied in a greenhouse vegetable (cucumber) plantation using an innovative system consisting of nanobubbles (NB_Se) and compared to that under conventional conditions of fertigation (C_Se) with six doses. The results revealed that NB_Se significantly reduced soil Se accumulation (38%-144%) and increased cucumber Se content compared with the C_Se treatments at the same Se dose. NB_Se significantly lowered the soil bacterial diversity, with an initial increase and then decrease with the Se doses. Bacterial associations and potential keystone taxa also differed between the NB_Se and C_Se. The greater abundance of oxidizing bacteria (indicated by the function composition of bacterial community) and the improved soil redox environment created by NBs sustained more available Se for plants, leading to a reduction in soil Se residual and an increase in the plant Se content. Our results highlight the feasibility and efficiency of NB_Se and demonstrate the important implications of Se for the maintenance of soil health and sustainability.

Social support moderates suicidal ideation among Chinese nursing home residents with limited activities of daily living and loneliness.

Rare studies are available exploring the impact of limited activities of daily living (ADL), loneliness on suicidal ideation, and protective effect of social support on their relationships in nursing home residents. This study aims to examine these links in a sample of older adults from nursing homes. A stratified random sampling was adopted to recruit 538 respondents from 37 nursing homes in Jinan. Suicidal ideation, limited ADL, social support and loneliness were assessed through instruments of Beck Suicidal Ideation Scale, ADL scale, Perceived Social Support Scale and UCLA Loneliness Scale. Relationships of latent variables were tested using Path Analysis in this cross-sectional study. The mediating effect of loneliness was significant on the association between limited ADL and suicidal ideation, and the mediation model was multiply moderated by social support with significant coefficients and acceptable model fitness. This study demonstrated the multiple moderating role of social support in the effect of limited ADL and loneliness on suicidal ideation among nursing home residents. More efforts are suggested in providing more available external resources to seniors' mental health for reducing risk of influencing factors of suicidal ideation.

Cost analysis of staged versus simultaneous bilateral total knee and hip arthroplasty using a propensity score matching.

BACKGROUND: Total joint arthroplasty (TJA), including total knee arthroplasty (TKA) and total hip arthroplasty (THA), is required for many patients. This study aimed to evaluate the medical costs, length of stay (LOS), blood transfusion and in-hospital complications in patients undergoing simultaneous and staged TJA. METHODS: All patients who underwent primary bilateral TJA from 2013 to 2018 in our institute were included. The propensity score matching analysis was performed between simultaneous and staged TJA patients. The difference in medical costs, LOS, blood transfusion and in-hospital complications was compared between simultaneous and staged groups. RESULTS: Except for materials fees and general therapy fees, medical costs (bed fees, general therapy fees, nursing care fees, check-up and laboratory test fees, surgical fees and drug fees) were significantly lower in the simultaneous TKA, THA and TJA group. The total average medical costs in simultaneous and staged TKA groups were $15 385 and $16 729 (p<0.001), respectively; THA groups were $14 503 and $16 142 (p=0.016), respectively; TJA groups were $15 389 and $16 830 (p<0.001), respectively. The highest and lowest costs were materials fees and nursing care fees. No significant differences were found for five common comorbidities and postoperative complications between the two subgroups. The simultaneous groups had a shorter LOS and the differences from the staged group for TKA, THA and the TJA group were 8, 6 and 8 days, respectively. The incidence of blood transfusion is higher for simultaneous groups and the difference from the staged group for TKA, THA and TJA is 32.69%, 18% and 29.3%, respectively. CONCLUSIONS: Our results indicate that simultaneous TKA and THA with a shorter LOS would cost fewer (costs incurred during hospitalisation) than staged TKA and THA. Complication rates were not affected by the choice for staged or simultaneous arthroplasty, but the incidence of blood transfusion was higher in the simultaneous groups.

Study of influential factors of provincial health expenditure -analysis of panel data after the 2009 healthcare reform in China.

BACKGROUND: Total Healthcare Expenditure (THE) has increased substantially in all countries. Since the health system reform and health policy environment differ from each country, it is necessary to analyze the motivations of THE in a specific country. METHODS: The objective of this study was to analyze the influential factors of Provincial THE (PTHE) per capita in China by using spatiotemporal panel data across 31 provinces (including provinces, autonomous regions, and municipalities, all called provinces in here) from 2009 to 2016 at the provincial and annual level. Generalized Estimating Equation (GEE) was used to identify the influential factors of PTHE per capita. RESULTS: The number of beds per 10,000 population explained most of the variation of PTHE per capita. The results also showed that health expenditure in China reacts more to mortality compared with the Gross Domestic Product (GDP) per capita. But mortality and Out-Of-Pocket Payments (OOP) as a percentage of THE were associated with PTHE per capita negatively. The rate of infectious diseases and THE as a percentage of GDP had no statistical significance. And the Proportion of the Population Aged 65 and Over (POP65) impact PTHE per capita positively. But the coefficient was small. CONCLUSIONS: In response to these findings, we conclude that the impact of the increasing percentage of OOP in THE diminishes the PTHE. Furthermore, we find that both the "baseline" health level and health provision are positively correlated with PTHE, which outweighs the effect of GDP.

The association between psychological strains and life satisfaction: Evidence from medical staff in China.

BACKGROUND: Previous research on the relationship between life satisfaction and its influencing factors has mainly focused on the work domain. Psychological strains, which result from these stress-related outcomes, have not been paid enough attention to explain how it correlates negatively with life satisfaction. METHOD: A cross-sectional study was conducted, using questionnaires sent to selected medical staff in a public hospital in Shandong, China (N = 1012). Multiple regression analysis was used to investigate how psychological strains influencing life satisfactions among medical staff. RESULTS: The findings indicate that aspiration strain and deprivation strain have significantly negative impact on medical staff's life satisfaction even with other variables controlled for. Weekly working hour was a significant predictor for life satisfaction. Family factors, such as marital status and kids in the family as well as social support were important factors in influencing individuals' life satisfaction. CONCLUSION: The current study highlights the negative associations between aspiration strain, deprivation strain and life satisfaction. The result underlines the importance of actions taken to prevent and combat psychological strains. It also provides some evidence for policy makers to improve the work environment for medical staff, such as reduce weekly working hours and enhance social support in order to increase medical staff's life satisfaction.

Testing the Strain Theory of Suicide - The Moderating Role of Social Support.

Background: According to the strain theory of suicide, strains, resulting from conflicting and competing pressures in an individual's life, are hypothesized to precede suicide. But social support is an important factor that can mitigate strains and lessen their input in suicidal behavior. Aims: This study was designed to assess the moderating role of social support in the relation between strain and suicidality. Methods: A sample of 1,051 employees were recruited in Beijing, the capital of China, through an online survey. Moderation analysis was performed using SPSS PROCESS Macro. Social support was measured with the Multidimensional Scale of Perceived Social Support, and strains were assessed with the Psychological Strains Scale. Results: Psychological strains are a good predictor of suicidality, and social support, a basic need for each human being, moderates and decreases the effects of psychological strains on suicidality. Limitations: The cross-sectional survey limited the extent to which conclusions about causal relationships can be drawn. Furthermore, the results may not be generalized to the whole of China because of its diversity. Conclusion: Social support has a tendency to mitigate the effects of psychological strains on suicidality.

Physical Illnesses and Medically Serious Suicide Attempts in Rural China.

Background: Physical illness is a strong correlate of suicidal behavior. However, its impact on attempted suicide in rural China is under-studied. Aims: This study was designed to assess the influence of physical illness on risk of suicide attempts and to identify distinguishing characteristics among medically serious suicide attempters with physical illnesses from those without. Method: Participants were medically serious suicide attempters identified through hospital emergency rooms (n = 659) as well as age- and gender-matched community controls (n = 659) from Shandong Province, China. Face-to-face interviews with the respondents were conducted with a semistructured protocol that included sociodemographic and psychological measures. Results: Physical illness was a significant risk factor for medically serious suicide attempts (OR = 1.739, 95% CI = 1.182-2.560). Older age was a significant risk factor, while female gender, no religion, and non-peasant occupations were significant protective factors for suicide attempts with physical illness. Limitations: Samples from only one province may not completely represent all serious suicide attempters in China. Furthermore, we did not assess the severity and the degree to which their physical illness limited the functioning of the individual. Conclusion: Physical illness is an important risk factor for suicide attempts in rural China. More efforts are needed to specifically target older people, males, those with religious beliefs, and peasant workers with physical illness.

Redox-based electron exchange capacity of biowaste-derived biochar accelerates syntrophic phenol oxidation for methanogenesis via direct interspecies electron transfer.

In this study, six different types of biochar (based on two feedstocks and three pyrolytic temperatures) were prepared as individual additives for both syntrophic phenol degradation and methanogenesis promotion. The results showed that for phenol degradation, the addition of biochar (15 g/L) shortened the methanogenic lag time from 15.0 days to 1.1-3.2 days and accelerated the maximum CH4 production rate from 4.0 mL/d to 10.4-13.9 mL/d. Microbial community analysis revealed that the electro-active Geobacter was enriched (from 3.8-7.7% to 11.1-23.1%), depending on the type of biochar that was added. This indicates a potential shift of syntrophic phenol metabolism from a thermodynamically unfavorable pathway with H2 as the interspecies electron transfer mediator to direct interspecies electron transfer (DIET). Integrated analysis of methanogenesis dynamics and the electrochemical properties of biochar showed that compared with electrical conductivity, the electron exchange capacity of biochar was more likely to dominate the DIET process, which was due to the presence of redox-active organic functional groups in biochar. The removal of biochar from the anaerobic system generally prolonged the lag time, revealing the importance of adsorption capacity of biochar to mitigate bio-toxicity of phenol to microbial activity.

Redox-active biochar facilitates potential electron tranfer between syntrophic partners to enhance anaerobic digestion under high organic loading rate.

Sawdust-based biochar prepared (SDBC) at three pyrolytic temperatures were compared as additives to mesophilic anaerobic digestion (AD). SDBC prepared at 500 °C performed better in enhancing CH4 production than other SDBCs. Analyzing the crucial electro-chemical characteristics of the SDBCs revealed that the excellent electron transfer capacity of SDBC was significant to stimulate methanogenesis promotion. A long-term semi-continuous operation further confirmed that adding SDBC to AD system increased the maximum organic loading rate (OLR) from 6.8 g VS/L/d to 16.2 g VS/L/d, which attributed to the extremely low volatile fatty acids (VFA) accumulation. Microbial community succession analysis found that SDBC addition altered both bacterial and archaea structure greatly. More importantly, the syntrophic and electro-active partners of Petrimonas and Methanosarcina synergistically enriched under high OLR condition were responsible for the high-efficient VFA degradation, which suggested that SDBC likely acted as redox-active mediator to facilitate direct interspecies electron transfer between the syntrophic partners for high-efficient syntrophic methanogenesis process.

A novel construct with biomechanical flexibility for articular cartilage regeneration.

BACKGROUND: Although tissue-engineered cartilage has been broadly studied, complete integration of regenerated cartilage with residual cartilage is still difficult for the inferior mechanical and biochemical feature of neocartilage. Chondrogenesis of mesenchymal stem cells can be induced by biophysical and biochemical factors. METHODS: In this study, autologous platelet-rich fibrin (PRF) membrane was used as a growth factor-rich scaffold that may facilitate differentiation of the transplanted bone marrow mesenchymal stem cells (BMSCs). At the same time, hydrostatic pressure was adopted for pre-adjustment of the seed cells before transplantation that may promote the mechanical flexibility of neocartilage. RESULTS: An in vitro study showed that the feasible hydrostatic pressure stimulation substantially promoted the chondrogenic potential of in vitro-cultured BMSC/PRF construct. In vivo results revealed that at every time point, the newborn tissues were the most favorable in the pressure-pretreated BMSC/PRF transplant group. Besides, the transplantation of feasible hydrostatic pressure-pretreated construct by BMSC sheet fragments and PRF granules could obviously improve the integration between the regenerated cartilage and host cartilage milieu, and thereby achieve boundaryless repair between the neocartilage and residual host cartilage tissue in rabbit temporomandibular joints. It could be concluded that feasible hydrostatic pressure may effectively promote the proliferation and chondrogenic differentiation of BMSCs in a BMSC/PRF construct. CONCLUSION: This newly formed construct with biomechanical flexibility showed a superior capacity for cartilage regeneration by promoting the mechanical properties and integration of neocartilage.

The role of anthrax toxin protein receptor 1 as a new mechanosensor molecule and its mechanotransduction in BMSCs under hydrostatic pressure.

Anthrax toxin protein receptor (ANTXR) 1 has many similarities to integrin and is regarded in some respects as a single-stranded integrin protein. However, it is not clear whether ANTXR1 responds to mechanical signals secondary to the activation of integrins or whether it is a completely new, independent and previously undiscovered mechanosensor that responds to an undefined subset of mechanical signaling molecules. Our study demonstrates that ANTXR1 is a novel mechanosensor on the cell membrane, acting independently from the classical mechanoreceptor molecule integrinß1. We show that bone marrow stromal cells (BMSCs) respond to the hydrostatic pressure towards chondrogenic differentiation partly through the glycogen synthase kinase (GSK) 3ß/ß-Catenin signaling pathway, which can be partly regulated by ANTXR1 and might be related to the direct binding between ANTXR1 and low-density lipoprotein receptor-related protein (LRP) 5/6. In addition, ANTXR1 specifically activates Smad2 and upregulates Smad4 expression to facilitate the transport of activated Smad2 to the nucleus to regulate chondrogenesis, which might be related to the direct binding between ANTXR1 and Actin/Fascin1. We also demonstrate that ANTXR1 binds to some extent with integrinß1, but this interaction does not affect the expression and function of either protein under pressure. Thus, we conclude that ANTXR1 plays a crucial role in BMSC mechanotransduction and controls specific signaling pathways that are distinct from those of integrin to influence the chondrogenic responses of BMSCs under hydrostatic pressure.

The Role of Depressive Symptoms in Suicide Attempt in Rural China.

Depression is a well-established predictor of suicidal behaviors, yet its effects among Chinese rural suicide attempters are understudied. In this study, we examined the role of depressive symptoms with other common risk factors, such as impulsivity, among medically serious suicide attempters. A case-controlled study was conducted in 13 rural counties in China. Medically serious suicide attempters (n = 791) and 791 nonsuicidal controls matched for sex and age range (±3 years) in the same location were recruited and interviewed to obtain information with regard to demographics, depressive symptom severity, and psychological strain measures. Both depressive symptoms, assessed with the Center for Epidemiological Studies Depression Scale, and psychological strains predicted suicide attempt risk, and psychological strains significantly predicted depressive symptoms. Depressive symptoms fully mediated the relations between value strain, deprivation strain, and suicide attempt risk, and partially mediated the associations among aspiration strain, coping strain, and suicide attempt risk. The reduction of psychological strains may help decrease both depression and suicidal behaviors.

Speciation, Distribution and Risk Assessment of Metals in Sediments from a Water Body Replenished by Effluent from a Wastewater Treatment Plant.

To assess the status and risk of metal pollution in landscape water body replenished by wastewater treatment plant effluent, the distribution of metals in sediments from three regions [regulation pond (RP), wetland lake (WL), and main lake (ML)] of Harbor Lake, Tianjin, China, was characterized. Higher levels of all metals (except Cr and Pb) were observed in RP sediments. As, Cr, Cu, Ni, Pb, and Zn were mainly bound to organic matter and sulfide (F3) and residual fractions (F4), while the exchangeable and carbonate fraction (F1) and Fe/Mn oxide fraction (F2) were the dominant forms for Cd. Additionally, finding showed that the overall risk of sediments in aquatic systems was affected by both metal toxicity and metal fractionation. Thus, according to a modified risk assessment code (RAC), the potential adverse effect of metals in sediments was medium (although As, Cd, Ni, and Zn had high mobility risks based on the RAC), decreasing in the order RP > WL > ML.