Effects of Alkali Elements on Copper Indium Gallium Aluminum Selenide Flexible Solar Cells Fabricated on Polyimide Substrates.

Polycrystalline Cu(InGaAl)Se2 (CIGAS) thin films were prepared on polyimide (PI) foils by depositing aluminum (Al) and CIGS precursor layers. Three ceramic CIGS quaternary targets with different sodium (Na) contents were used for investigating the influences of alkali doping at an annealing temperature of 500 °C. The Al concentration was enriched at the front interfaces of absorber films with different Na doping amounts after annealing. Na in the precursor layer diffused to both interfaces during the annealing process, most Na diffused into the Mo layer, and Na existed in the annealed film as compounds Na2Sex and Na2SeO3. An appropriate amount Na element could be beneficial for grain growth in the region beneath the surface. Low Na doping had no significant effect on the crystallization property. High Na doping effected the diffusion of the Cu2-xSe liquid phase and reduced the grain size. On the basis of good crystallization, the passivation effect of Na can effectively improve the performance of cells. A certified power conversion efficiency of 16.19% of a CIGAS flexible solar cell with a 0.54 cm2 active area has been achieved.

Preparation of 3D printed silicon nitride bioceramics by microwave sintering.

Silicon nitride (Si3N4) is a bioceramic material with potential applications. Customization and high reliability are the foundation for the widespread application of Si3N4 bioceramics. This study constructed a new microwave heating structure and successfully prepared 3D printed dense Si3N4 materials, overcoming the adverse effects of a large amount of 3D printed organic forming agents on degreasing and sintering processes, further improving the comprehensive performance of Si3N4 materials. Compared with control materials, the 3D printed Si3N4 materials by microwave sintering have the best mechanical performance: bending strength is 928 MPa, fracture toughness is 9.61 MPa·m1/2. Meanwhile, it has the best biocompatibility and antibacterial properties, and cells exhibit the best activity on the material surface. Research has shown that the excellent mechanical performance and biological activity of materials are mainly related to the high-quality degreasing, high cleanliness sintering environment, and high-quality liquid-phase sintering of materials in microwave environments.

Fabrication and optimization of bioelectrochemical system using tetracycline-degrading bacterial strains for antibiotic wastewater treatment.

In this study, a microbial fuel cell was constructed using Raoultella sp. XY-1 to efficiently degrade tetracycline (TC) and assess the effectiveness of the electrochemical system. The degradation rate reached 83.2 ± 1.8 % during the 7-day period, in which the system contained 30 mg/L TC, and the degradation pathway and intermediates were identified. Low concentrations of TC enhanced anodic biofilm power production, while high concentrations of TC decreased the electrochemical activity of the biofilm, extracellular polymeric substances, and enzymatic activities associated with electron transfer. Introducing electrogenic bacteria improved power generation efficiency. A three-strain hybrid system was fabricated using Castellaniella sp. A3, Castellaniella sp. A5 and Raoultella sp. XY-1, leading to the enhanced TC degradation rate of 90.4 % and the increased maximum output voltage from 200 to 265 mV. This study presents a strategy utilizing tetracycline-degrading bacteria as bioanodes for TC removal, while incorporating electrogenic bacteria to enhance electricity generation.

Joint effect of atrial fibrillation and obesity on mortality in critically ill patients.

BACKGROUND: The interplay between atrial fibrillation (AF) and obesity on mortality in critically ill patients warrants detailed exploration, given their individual impacts on patient prognosis. This study aimed to assess the associations between AF, obesity, and 1-year mortality in a critically ill population. METHODS: Utilizing data from the Medical Information Mart for Intensive Care (MIMIC)-IV database, we conducted a retrospective analysis of adult patients admitted to the intensive care unit. The primary endpoint was 1-year mortality, analyzed through Cox regression with hazard ratio (HR) and Kaplan-Meier survival methods. RESULTS: The study included 25,654 patients (median age 67.0 years, 40.6% female), with 39.0% having AF and 36.1% being obese. Multivariate COX regression analysis revealed that AF was associated with a 14.7% increase in the risk of 1-year mortality (p < 0.001), while obesity was linked to a 13.9% reduction in mortality risk (p < 0.001). The protective effect of obesity on mortality was similar in patients with (HR = 0.85) and without AF (HR = 0.86). AF led to a slightly higher risk of mortality in patients without obesity (HR = 1.16) compared to those with obesity (HR = 1.13). Kaplan-Meier survival curves highlighted that non-obese patients with AF had the lowest survival rate, whereas the highest survival was observed in obese patients without AF. CONCLUSIONS: AF significantly increased 1-year mortality risk in critically ill patients, whereas obesity was associated with a decreased mortality risk. The most adverse survival outcomes were identified in non-obese patients with AF.

Sensory ASIC3 channel exacerbates psoriatic inflammation via a neurogenic pathway in female mice.

Psoriasis is an immune-mediated skin disease associated with neurogenic inflammation, but the underlying molecular mechanism remains unclear. We demonstrate here that acid-sensing ion channel 3 (ASIC3) exacerbates psoriatic inflammation through a sensory neurogenic pathway. Global or nociceptor-specific Asic3 knockout (KO) in female mice alleviates imiquimod-induced psoriatic acanthosis and type 17 inflammation to the same extent as nociceptor ablation. However, ASIC3 is dispensable for IL-23-induced psoriatic inflammation that bypasses the need for nociceptors. Mechanistically, ASIC3 activation induces the activity-dependent release of calcitonin gene-related peptide (CGRP) from sensory neurons to promote neurogenic inflammation. Botulinum neurotoxin A and CGRP antagonists prevent sensory neuron-mediated exacerbation of psoriatic inflammation to similar extents as Asic3 KO. In contrast, replenishing CGRP in the skin of Asic3 KO mice restores the inflammatory response. These findings establish sensory ASIC3 as a critical constituent in psoriatic inflammation, and a promising target for neurogenic inflammation management.

Effect of cognitive behavioral therapy on pain, knee function, and psychological status in patients after primary total knee arthroplasty: a systematic review and meta-analysis.

OBJECTIVE: The clinical efficacy of cognitive behavioral therapy (CBT) after Total knee arthroplasty (TKA) is still controversial, and the purpose of this meta-analysis was to evaluate the effect of CBT on pain, knee function, and psychological status of patients after TKA. METHODS: We systematically searched electronic databases such as CNKI, CBM, VIP, PubMed, Cochrane Library, and EMBASE for randomized controlled studies up to February 30, 2023. Screening against inclusion criteria to select valid studies and extract data. The quality of included studies was evaluated by the Cochrane Collaboration risk-of-bias 2 (RoB 2) tool for randomized trials. Statistical analysis of the data from this study was carried out using Stata 15.1 software. RESULTS: Finally, our meta-analysis incorporated seven randomized controlled studies of high quality, including 608 patients. The findings of the meta-analysis demonstrated a noteworthy decrease in kinesiophobia levels during the early postoperative phase in the CBT group as compared to the usual care group (WMD = -6.35, 95% CI: -7.98 to -4.72, Z = 7.64, P < 0.001). However, no statistically significant difference between the CBT and usual care groups in terms of postoperative pain as well as knee function. CONCLUSION: CBT may effectively reduce the level of kinesiophobia in the short term after TKA, but did not significantly relieve knee pain or improve knee function.

Exploration of the Vermiculite-Induced Bacterial Community and Co-Network Successions during Sludge-Waste Mushroom Co-Composting.

Vermiculite is a clay mineral with unique physical properties that plays a significant role in plant cultivation, soil remediation, and solid waste management. In this research, we first explored how vermiculite-to-microbe interactions evolved during sludge-waste mushroom residue co-composting. Vermiculite's addition had a substantial impact on the microbial α and ß diversities, significantly changed the microbial community pattern, and strengthened the composting nutrient circulation through the formation of more specialist and generalist species. The microbial community characteristics exhibited common co-networks for resisting composting environment stresses. Vermiculite contributed to enhancing the keystone taxa Proteobacteria and Actinobacteriota and caused the ecological function network to diversify in the warming and maturation phases, with more complexity and tightness in the thermophilic phase (with super-generalist species existing). The enhanced microbial interactions induced by vermiculite possessed a greater capacity to facilitate the metabolisms of carbohydrates and amino acids and cellulolysis, thereby promoting composting humification, and nitrogen retention in the final compost and composting maturity. These findings are helpful for us to understand the biological process mechanisms of the effect of vermiculite additives on composting and contribute to the establishment of a theoretical framework for enhancing the microbial interactions in composting systems by adding vermiculite in practical applications.

Acid and Glutathione Dual-Responsive, Injectable and Self-Healing Hydrogels for Controlled Drug Delivery.

Considering the complexity of physiological microenvironments and the risks of surgical infection, there still remains critical demand to develop a hydrogel as a drug release platform with multifunctional properties, including good neutral stability and sensitive multiple stimuli-responsive behaviors, as well as injectable and self-healing properties. Herein, we present a facile preparation of injectable, self-healing hydrogels with acid and glutathione (GSH) dual-responsiveness for controlled drug delivery. Initially, the anticancer drug camptothecin (CPT) was premodified with disulfide bonds and attached to poly(ethylenimine) (PEI) via the Schiff base reaction, resulting in PEI-CPT. Subsequently, OSA-IR780 was synthesized through the Schiff base reaction involving IR780 with amine groups (IR780-NH2) and oxidized sodium alginate with aldehyde groups (OSA). The formation of PEI-CPT/OSA-IR780 hydrogels with various solid contents occurred rapidly within 40 s through a simple mixing process of the aqueous solution of PEI-CPT and OSA-IR780. These hydrogels exhibited remarkable stability under neutral conditions and controlled release of CPT upon exposure to simulated tumor environments characterized by acidic conditions and elevated GSH concentrations. Furthermore, they had significant injectable and self-healing properties due to the dynamically imine-cross-linked networks. In addition, the prepared hydrogels exhibited long-term biodegradability and biocompatibility. Collectively, these features indicate the great potential of PEI-CPT/OSA-IR780 hydrogels as therapeutic delivery vehicles.

Extracellular proteins enhance Cupriavidus pauculus nickel tolerance and cell aggregate formation.

Heavy metal-resistant bacteria secrete extracellular proteins (e-PNs). However, the role of e-PNs in heavy metal resistance remains elusive. Here Fourier Transform Infrared Spectroscopy implied that N-H, C = O and NH2-R played a crucial role in the adsorption and resistance of Ni2+ in the model organism Cuprividus pauculus 1490 (C. pauculus). Proteinase K treatment reduced Ni2+ resistance of C. pauculus underlining the essential role of e-PNs. Further three-dimension excitation-emission matrix fluorescence spectroscopy analysis demonstrated that tryptophan proteins as part of the e-PNs increased significantly with Ni2+ treatment. Proteomic and quantitative real-time polymerase chain reaction data indicated that major changes were induced in the metabolism of C. pauculus in response to Ni2+. Among those lipopolysaccharide biosynthesis, general secretion pathways, Ni2+-affiliated transporters and multidrug efflux play an essential role in Ni2+ resistance. Altogether the results provide a conceptual model for comprehending how e-PNs contribute to bacterial resistance and adsorption of Ni2+.

Accuracy and reproducibility of two-dimensional computed tomography-based positioning of femoral component rotational alignment in preoperative planning for total knee arthroplasty.

BACKGROUND: Poor rotation of the femoral component in total knee arthroplasty (TKA) can result in various postoperative complications, underscoring the critical importance of preoperative planning. PURPOSE: To improve the accuracy of femoral component positioning during TKA, this study compared the accuracy and repeatability of different two-dimensional (2D) computed tomography (CT) measurement methods for measuring the posterior condylar angle (PCA) in preoperative TKA planning. METHODS: A retrospective analysis was conducted on 75 patients (150 knees) who underwent bilateral lower extremity computed tomography angiography (CTA) at Fuyang People's Hospital from January 2021 to July 2021. Three different methods were used to measure the PCA based on 2D CT images (axial CT slices) and three-dimensional(3D) models (femoral models reconstructed from CT data) in this study. Method 1: Single-plane 2D CT measurement, measuring PCA in the most obvious single-plane CT slice of the surgical transepicondylar axis (sTEA); Method 2: multi-plane 2D CT measurement, identifying and locating anatomical landmarks in multiple 2D CT slices and measuring PCA; Method 3: 3D model measurement, measuring PCA in the reconstructed femur 3D model. Compare the differences in PCA measurements between the three measurement methods. A positive PCA measurement was recorded when the sTEA was externally rotated relative to the posterior condylar line (PCL). Any difference exceeding 3° between the PCA measurement in the 2D CT and the PCA reference value in the 3D model was classified as an outlier. The intraclass correlation coefficient (ICC) and Bland-Altman method were utilized to assess the intra- and inter-observer reproducibility of the three measurement methods. RESULTS: The PCA measurement in the single-plane 2D CT was 1.91 ± 1.94°, with a measurement error of - 1.22 ± 1.32° and 12.7% of outlier values. In the multi-plane 2D CT, the PCA measurement was 2.96 ± 1.68°, with a measurement error of -0.15 ± 0.91° and 6.0% of outlier values. The PCA measurement in the 3D model was 3.12 ± 1.69°. The PCA measurement in single-plane 2D CT was notably smaller than that in multi-plane 2D CT and 3D models, with no significant difference between the latter two. The multi-plane 2D CT showed significantly lower measurement error and outlier values than the single-plane 2D CT. All three PCA measurement methods exhibited high reproducibility (ICC: 0.93 ~ 0.97). CONCLUSIONS: Using of multi-plane 2D CT for measuring PCA in preoperative planning of TKA has high reproducibility and accuracy, with fewer outlier values. We recommend preoperative measurement of PCA using muti-plane 2D CT to improve the accuracy of positioning the femoral component rotational alignment during surgery.

Silicon Nitride Bioceramics Sintered by Microwave Exhibit Excellent Mechanical Properties, Cytocompatibility In Vitro, and Anti-Bacterial Properties.

Silicon nitride is a bioceramic with great potential, and multiple studies have demonstrated its biocompatibility and antibacterial properties. In this study, silicon nitride was prepared by a microwave sintering technique that was different from common production methods. SEM and pore distribution analysis revealed the microstructure of microwave-sintered silicon nitride with obvious pores. Mechanical performance analysis shows that microwave sintering can improve the mechanical properties of silicon nitride. The CCK-8 method was used to demonstrate that microwave-sintered silicon nitride has no cytotoxicity and good cytocompatibility. From SEM and CLSM observations, it was observed that there was good adhesion and cross-linking of cells during microwave-sintered silicon nitride, and the morphology of the cytoskeleton was good. Microwave-sintered silicon nitride has been proven to be non-cytotoxic. In addition, the antibacterial ability of microwave-sintered silicon nitride against Staphylococcus aureus and Escherichia coli was tested, proving that it has a good antibacterial ability similar to the silicon nitride prepared by commonly used processes. Compared with silicon nitride prepared by gas pressure sintering technology, microwave-sintered silicon nitride has excellent performance in mechanical properties, cell compatibility, and antibacterial properties. This indicates its enormous potential as a substitute material for manufacturing bone implants.

Pulse-shaped high-energy and high-average-power fiber laser in the nanosecond regime.

A high-energy and high-average-power pulsed fiber laser has been investigated in a master oscillator power amplifier (MOPA) configuration seeding with a diode laser at a programmed pulse duration of ∼250ns. The fiber amplifier successfully demonstrates the pulse with 21.4 mJ at the repetition rate of 50 kHz and a maximum average output power of 1535 W with a slope efficiency of 81.6% at 250 kHz. To overcome fiber nonlinearities such as stimulated Raman scattering (SRS) and self-phase modulation (SPM), extra-large mode area ytterbium (Yb)-doped step-index dual cladding fiber has been utilized as gain fiber in the MOPA laser system. The gain saturation effect in the power amplifier was greatly mitigated by the programmed seed signal. This pulse-shaped MOPA system can provide practical applications in many fields such as laser cleaning, paint stripping, and other applications requiring special pulse shapes.

A Survey of Universal Quantum von Neumann Architecture.

The existence of universal quantum computers has been theoretically well established. However, building up a real quantum computer system not only relies on the theory of universality, but also needs methods to satisfy requirements on other features, such as programmability, modularity, scalability, etc. To this end, here we study the recently proposed model of quantum von Neumann architecture by putting it in a practical and broader setting, namely, the hierarchical design of a computer system. We analyze the structures of quantum CPU and quantum control units and draw their connections with computational advantages. We also point out that a recent demonstration of our model would require less than 20 qubits.

Implantable Electrochemical Sensors for Brain Research.

Implantable electrochemical sensors provide reliable tools for in vivo brain research. Recent advances in electrode surface design and high-precision fabrication of devices led to significant developments in selectivity, reversibility, quantitative detection, stability, and compatibility of other methods, which enabled electrochemical sensors to provide molecular-scale research tools for dissecting the mechanisms of the brain. In this Perspective, we summarize the contribution of these advances to brain research and provide an outlook on the development of the next generation of electrochemical sensors for the brain.

In Vivo Electrochemical Biosensors: Recent Advances in Molecular Design, Electrode Materials, and Electrochemical Devices.

Electrochemical biosensors provide powerful tools for dissecting the dynamically changing neurochemical signals in the living brain, which contribute to the insight into the physiological and pathological processes of the brain, due to their high spatial and temporal resolutions. Recent advances in the integration of in vivo electrochemical sensors with cross-disciplinary advances have reinvigorated the development of in vivo sensors with even better performance. In this Review, we summarize the recent advances in molecular design, electrode materials, and electrochemical devices for in vivo electrochemical sensors from molecular to macroscopic dimensions, highlighting the methods to obtain high performance for fulfilling the requirements for determination in the complex brain through flexible and smart design of molecules, materials, and devices. Also, we look forward to the development of next-generation in vivo electrochemical biosensors.

Evaluation of fungal community assembly and function during food waste composting with Aneurinibacillus sp. LD3 inoculant.

The objective of this work was to evaluate the fungal community assembly and function during food waste composting with Aneurinibacillus sp. LD3 (LD3) inoculant. Inoculation reduced the content of total organic carbon, moisture content, nitrate nitrogen, and nitrite nitrogen. The LD3 inoculant was able to drive the changes in the assembly of the fungal community. In particular, inoculation with LD3 not only increased the relative abundance of Ascomycota and Trichocomaceae_unclassified for lignocellulose degradation at the mesophilic and cooling stages but also reduced the relative abundances of the opportunistic human pathogen Candida. Saprotroph was the predominant fungal trophic mode in composting, and inoculation with LD3 has a better inactivation effect on animal and plant pathogenic fungi during composting. Furthermore, the variation of the fungal community after inoculation with LD3 was the largest explained by temperature (30.64%). These results implied that LD3 significantly regulated fungal composition and function of food waste composting.

Real-Time Tracking of Electrical Signals and an Accurate Quantification of Chemical Signals with Long-Term Stability in the Live Brain.

The development of in vivo analytical tools and methods for recording electrical signals and accurately quantifying chemical signals is a key issue for a comprehensive understanding of brain events. The electrophysiological microelectrode was invented to monitor electrical signals in free-moving brains. On the other hand, electrochemical assays with excellent spatiotemporal resolution provide an effect way to monitor chemical signals in vivo. Unfortunately, the in vivo electrochemical biosensors still have three limitations. First, many biological species such as reactive oxygen species (ROS) and neurotransmitters demonstrate large overpotentials at conventional electrodes. Thus, it is hard to convert the chemical/electrochemical signals of these molecules into electric signals. Second, the interfacial properties of the recognition molecules assembled onto the electrode surfaces have a great influence on the transmission of electric charge through the interface and the stability of the modified recognition molecules. Meanwhile, the surface of biosensors implanted in the brain is easily absorbed by many proteins present in the brain, resulting in the loss of signals. Finally, activities in the brain including neuron discharges and electrophysiological signals may be affected by electrochemical measurements due to the application of extra potentials and/or currents.This Account presents a deep view of the fundamental design principles and solutions in response to the above challenges for developing in vivo biosensors with high performance while meeting the growing requirements, including high selectivity, long-time stability, and simultaneously monitoring electrical and chemical signals. We aim to highlight the basic criteria based on a double-recognition strategy for the selective biosensing of ROS, H2S, and HnS through the rational design of specific recognition molecules followed by electrochemical oxidation or reduction. Recent developments in designing functionalized surfaces through a systematic investigation of self-assembly with Au-S bonds, Au-Se bonds, and Au≡C bonds for facilitating electrochemical properties as well as improving the stability are summarized. More importantly, this Account highlights the novel methodologies for simultaneously monitoring electrical and chemical signals ascribed to the dynamic changes in K+, Na+, and Ca2+ and pH values in vivo. Additionally, SERS-based photophysiological microarray probes have been developed for quantitatively tracking chemical changes in the live brain together with recording electrophysiological signals.The design principles and novel strategies presented in this Account can be extended to the real-time tracking of electrical signals and the accurate quantification of more chemical signals such as amino acids, neurotransmitters, and proteins to understand the brain events. The final part also outlines potential future directions in constructing high-density microarrays, eventually enabling the large-scale dynamic recording of the chemical expression of multineuronal signals across the whole brain. There is still room to develop a multifiber microarray which can be coupled with photometric methods to record chemical signals both inside and outside neurons in the live brains of freely moving animals to understand physiological processes and screen drugs.

Exploring the dynamic of microbial community and metabolic function in food waste composting amended with traditional Chinese medicine residues.

The aim of this study was to explore the dynamic of microbial community and metabolic function in food waste composting amended with traditional Chinese medicine residues (TCMRs). Results suggested that TCMRs addition at up to 10% leads to a higher peak temperature (60.5 °C), germination index (GI) value (119.26%), and a greater reduction in total organic carbon (TOC) content (8.08%). 10% TCMRs significantly induced the fluctuation of bacterial community composition, as well as the fungal community in the thermophilic phase. The addition of 10% TCMRs enhanced the abundance of bacterial genera such as Acetobacter, Bacillus, and Brevundimonas, as well as fungal genera such as Chaetomium, Thermascus, and Coprinopsis, which accelerated lignocellulose degradation and humification degree. Conversely, the growth of Lactobacillus and Pseudomonas was inhibited by 10% TCMRs to weaken the acidic environment and reduce nitrogen loss. Metabolic function analysis revealed that 10% TCMRs promoted the metabolism of carbohydrate and amino acid, especially citrate cycle, glycolysis/gluconeogenesis, and cysteine and methionine metabolism. Redundancy analysis showed that the carbon to nitrogen (C/N) ratio was the most significant environmental factor influencing the dynamic of bacterial and fungal communities.

Isolation and characterization of a novel thermotolerant alkali lignin-degrading bacterium Aneurinibacillus sp. LD3 and its application in food waste composting.

The aim of this study was to isolate thermotolerant alkali lignin-degrading bacteria and to investigate their degradation characteristics and application in food waste composting. Two thermotolerant alkali lignin-degrading bacteria isolates were identified as Bacillus sp. LD2 (LD2) and a novel species Aneurinibacillus sp. LD3 (LD3). Compared with strain LD2, LD3 had a higher alkali lignin degradation rate (61.28%) and ligninolytic enzyme activities, and the maximum lignin peroxidase, laccase, and manganese peroxidase activities were 3117.25, 1484.5, and 1770.75 U L-1, respectively. GC-MS analysis revealed that low-molecular-weight compounds such as 4'-hydroxy-3'-methoxy acetophenone, vanillic acid, 1-(4-hydroxy-3,5-dimethoxyphenyl), benzoic acid, and octadecanoic acid were formed in the degradation of alkali lignin by LD3, indicating the cleavage of ß-aryl ether, Cα-Cß bonds, and aromatic rings in lignin. Composting results showed that inoculating LD3 improved the degradation of organic matter by 20.11% and reduced the carbon-to-nitrogen (C/N) ratio (15.66). Additionally, a higher decrease in the content of lignocellulose was observed in the LD treatment. FTIR and 3D-EEM spectra analysis indicated that inoculating LD3 promoted the decomposition of easily available organic substances and lignocellulose and the formation of aromatic structures and humic acid-like substances. In brief, the thermotolerant lignin-degrading bacterium Aneurinibacillus sp. LD3 is effective in degrading lignin and improving the quality of composting.

Effect of nickel (II) on the performance of anodic electroactive biofilms in bioelectrochemical systems.

The impact of nickel (Ni2+) on the performance of anodic electroactive biofilms (EABs) in the bioelectrochemical system (BES) was investigated in this study. Although it has been reported that Ni2+ influences microorganisms in a number of ways, it is unknown how its presence in the anode of a BES affects extracellular electron transfer (EET) of EABs, microbial viability, and the bacterial community. Results revealed that the addition of Ni2+ decreased power output from 673.24 ± 12.40 mW/m2 at 0 mg/L to 179.26 ± 9.05 mW/m2 at 80 mg/L. The metal and chemical oxygen demand removal efficiencies of the microbial fuel cells (MFCs) declined as Ni2+ concentration increased, which could be attributed to decreased microbial viability as revealed by SEM and CLSM. FTIR analysis revealed the involvement of various microbial biofilm functional groups, including hydroxyl, amides, methyl, amine, and carboxyl, in the uptake of Ni2+. The presence of Ni2+ on the anodic biofilms was confirmed by SEM-EDS and XPS analyses. CV demonstrated that the electron transfer performance of the anodic biofilms was negatively correlated with the various Ni2+ concentrations. EIS showed that the internal resistance of the MFCs increased with increasing Ni2+ concentration, resulting in a decrease in power output. High-throughput sequencing results revealed a decrease in Geobacter and an increase in Desulfovibrio in response to Ni2+ concentrations of 10, 20, 40, and 80 mg/L. Furthermore, the various Ni2+ concentrations decreased the expression of EET-related genes. The Ni2+-fed MFCs had a higher abundance of the nikR gene than the control group, which was important for Ni2+ resistance. This work advances our understanding of Ni2+ inhibition on EABs, as well as the concurrent removal of organic matter and Ni2+ from wastewater.