Perillaldehyde ameliorates lipopolysaccharide-induced acute lung injury via suppressing the cGAS/STING signaling pathway.

Acute lung injury (ALI) is a common life-threatening illness characterized by a lung inflammatory response and oxidative stress, and effective agent therapies are currently lacking. mtDNA can be recognized by cGAS/STING, the dysregulation of which leads to inflammatory diseases, such as ALI. Perillaldehyde(PAH), one of the major active components of traditional Chinese medicine made from Perilla frutescens, has antioxidant and antiinflammatory effects. The aim of this study was to explore whether PAH can protect against lipopolysaccharide (LPS)-induced ALI and whether its protective effect is exerted through the regulation of cGAS/STING signaling. We found that PAH significantly inhibited lung histological changes, inflammatory cell infiltration, and the overproduction of inflammatory cytokines induced by LPS. Moreover, PAH inhibited LPS-induced oxidative stress, as shown by the deceases in superoxide dismutase (SOD) and glutathione(GSH) levels and increased in malondialdehyde (MDA) and lactate dehydrogenase (LDH) levels. In addition, PAH markedly downregulated the expression of cGAS, STING, p-TBK, p-IRF3, p-P65, and p-IκB, and pharmacological inhibition of cGAS/STING inhibited ALI- induced by LPS. Furthermore, the levels of mitochondrial ROS (mROS) and mtDNA were increased, and cGAS/STING-mediated IRF3/NF-κB signaling was activated during the inflammatory response- induced by LPS in RAW264.7 cells. In addition, pretreatment with the STING activator partially abolished the inhibitory effect of PAH on the inflammation and activation of STING-mediated IRF3/NF-κB signaling induced by LPS. Overall, the results revealed that PAH can effectively alleviate ALI by inhibiting cGAS/STING-mediated IRF3/NF-κB signaling, and that PAH may be a potential candidate agent for the treatment of ALI.

Case report: Two rare new cases of diaphragmatic bronchogenic cysts.

The bronchogenic cyst (BC) is a type of congenital anomaly that is most usually found in the mediastinum and lung, and rarely in the diaphragm. We report two cases of bronchogenic cysts originating from the left diaphragm. Case 1 involved a 50-year-old man who underwent computed tomography (CT) of the adrenal glands for hypertension, showing left adrenal changes. An adrenal CT at our hospital showed a space-occupying lesion above the left diaphragm. We performed a left-sided thoracotomy in the seventh intercostal space and found that the mass was entirely located in the diaphragm. Case 2 was a 58-year-old woman who underwent chest CT under a standard health check-up, and a subpleural lesion of approximately 4 cm was found in the posterior basal segment of the left inferior lung lobe. During thoracoscopic surgery, the mass was found to originate from the surface of the diaphragm, having no correlation with the lung tissue. The two cases of diaphragmatic bronchogenic cysts were confirmed by surgical pathology. Ectopic bronchogenic cysts arising from the diaphragm are very uncommon, and diaphragmatic bronchogenic cysts typically occur on the left side, in women, and in the angle between the vertebral column and the diaphragm. As far as we know, there are no relevant reports of intradiaphragmatic BCs similar to case 1, which was entirely located within the diaphragm. Most diaphragmatic BCs are located on the surface of the diaphragm and project toward the thoracic or abdominal cavities. Moreover, bilocular diaphragmatic bronchogenic cysts, similar to case 2, have not yet been reported.

Understanding impacts of cropland pattern dynamics on grain production in China: A integrated analysis by fusing statistical data and satellite-observed data.

Detailed information on spatial distribution of croplands and grain yields is crucial for agricultural management and food security, but is often limited by a lack of geospatial data. By integrating satellite observation and statistical data, this study produced new geospatial data of cropland areas and grain yields in China during 2000-2020. We found that the decrease of relatively high-yielding croplands in southern China mainly caused by the expansion of constructed land. Yet, the increase of croplands largely occurred in temperature/water-limited regions of Northern Arid and Semiarid Region (NASR) and Northeast China Plain (NCP). Croplands' decrease in southern China and expansion in NCP and NASR jointly led to the continuous northward shift of the centre of gravity of croplands and grain yields. This spatial transfer of croplands resulted in relatively lower-than- average grain yield per unit area (AGYA) croplands decreasing from 38.96% (2000) to 35.75% (2020), but also relatively higher-than-AGYA croplands decreasing from 38.41% (2000) to 35.01% (2020), implying spatial challenges of grain production. Generally, every 1 km2 of cropland loss in traditional high-yield zones required nearly 1-3 times expansion in area in NASR and NCP to balance grain yield losses. The new geospatial data and these findings from this study could provide valuable information for regional agriculture development and policy marking.

Verification of a Stiffness-Variable Control System with Feed-Forward Predictive Earthquake Energy Analysis.

Semi-active isolation systems with controllable stiffness have been widely developed in the field of seismic mitigation. Most systems with controllable stiffness perform more robustly and effectively for far-field earthquakes than for near-fault earthquakes. Consequently, a comprehensive system that provides comparable reductions in seismic responses to both near-fault and far-field excitations is required. In this regard, a new algorithm called Feed-Forward Predictive Earthquake Energy Analysis (FPEEA) is proposed to identify the ground motion characteristics of and reduce the structural responses to earthquakes. The energy distribution of the seismic velocity spectrum is considered, and the balance between the kinetic energy and potential energy is optimized to reduce the seismic energy. To demonstrate the performance of the FPEEA algorithm, a two-degree-of-freedom structure was used as the benchmark in the numerical simulation. The peak structural responses under two near-fault and far-field earthquakes of different earthquake intensities were simulated. The isolation layer displacement was suppressed most by the FPEEA, which outperformed the other three control methods. Moreover, superior control on superstructure acceleration was also supported by the FPEEA. Experimental verification was then conducted with shaking table test, and the satisfactory performance of the FPEEA on both isolation layer displacement and superstructure acceleration was demonstrated again. In summary, the proposed FPEEA has potential for practical application to unexpected near-fault and far-field earthquakes.

Impacts of climatic warming on cropping system borders of China and potential adaptation strategies for regional agriculture development.

Climate warming and its corresponding impacts on agriculture system increasingly attach great attentions. Earlier studies more concerned the impacts of the cultivated area expansion under climate change. Yet limited knowledge is about the impacts of climate warming on the cropping index change with the shifts of cropping system border. In this study, we used climatic data (1961-2015) to firstly investigate impacts of warming temperature on potential cropping system border expansion of China, and further used agricultural statistical data and satellite-based land use data to analyze the response of current land system to potential cropping system border expansion. Results of this study indicated that obviously advanced SDT10 and prolonged EDT10 contributed to the 88.4% regions of increased AAT10 at the past half century. Moreover, the northward expansion of the suitable cultivated areas in different cropping systems provided advantages for potential multiple cropping index (PMCI) improvement. Unfortunately, this study found that a significantly declined multiple cropping index (MCI) was observed in the peri-urban regions and the provinces with large out-migration of agriculture labor. The evidently increased MCI was only greatly observed in Xinjiang province. Besides, the potential increment of multiple cropping index (PIMCI) for different cropping system border expansion regions presented a rising trend and reached 53.6% in 2015 due to warming climate. Particularly, the significantly increased PIMCI was observed in the Loess Plateau, the Inner Mongolia, the Middle-lower Yangtze Plain, Northeast China Plain, Southern China and Beijing-Tianjin-Hebei Metropolitan Region. However, the response of current land system to the changes of PMCI and PIMCI was not timely. Based on the findings of our study, some potential agriculture development strategies were suggested by comprehensively considering regional natural conditions, agricultural production conditions and socioeconomic conditions. We hope these findings of this study could provide some valuable information for agricultural development policy decision-making.

LINC01619 promotes non-small cell lung cancer development via regulating PAX6 by suppressing microRNA-129-5p.

This article explored LINC01619 impact on non-small cell lung cancer (NSCLS) development. LINC01619 expression in tumor tissues/normal tissues of NSCLS patients was detected by qRT-PCR and in situ hybridization. PAX6 expression in clinical tissues was researched by immunohistochemistry. After transfection, SPCA1 and A549 cells were subjected to CCK-8 assay and cell colony formation experiment. Xenograft tumor experiment was conducted. ALDH+ cells from SPCA1 and A549 cells were separated and transfected. ALDH+ cells percentage, sphere number and cancer stem cell markers expression was determined by flow cytometry, sphere culture and Western blot respectively. Luciferase reporter gene assay and RNA binding protein immunoprecipitation assay was conducted. The colocalization of LINC01619 and miR-129-5p in cells was determined by RNA fluorescence in situ hybridization experiment. Gene expression in tissues and cells were assessed by qRT-PCR and Western blot. As a result, aberrantly up-regulated LINC01619 and PAX6 in NSCLC patients predicted poor prognosis. LINC01619 overexpression in SPCA1 cells enhanced cell viability, cloning ability, and xenograft tumors volume and weigh, whereas LINC01619 silencing in A549 cells weakened the above indicators. LINC01619 overexpression promoted cancer stem cells characteristics including increasing percentage of ALDH+ cells, sphere number and cancer stem cell markers expression. LINC01619 directly inhibited miR-129-5p and the two genes were mainly colocalized in the cytoplasm. PAX6 was up-regulated in NSCLC and directly suppressed by miR-129-5p. LINC01619 promoted cells viability, cloning ability and cancer stem cells characteristics in NSCLC via the miR-129-5p/PAX6 axis. Thus, LINC01619 promotes NSCLC development via regulating PAX6 by suppressing miR-129-5p.

LINC00210 plays oncogenic roles in non-small cell lung cancer by sponging microRNA-328-5p.

Long noncoding RNA (lncRNA) has an important role in regulating non-small cell lung cancer (NSCLC) progression. The present study aimed to investigate the effect of LINC00210 in NSCLC progression in order to provide a novel treatment target for patients with NSCLC. A total of 39 NSCLC patients were obtained and divided into LINC00210 high expression and low expression groups. Subsequently, the 5-year survival rate from this patient cohort was analyzed. The SK-MES-1 and A549 NSCLC and the human 16-HBE bronchial epithelial cell lines were utilized to investigate expression level of LIN00210. A549 cells were used to investigate cell proliferation, migration and invasive abilities using Cell Counting kit 8, Transwell and Matrigel assays, respectively. In addition, the luciferase reporter gene assay was performed to investigate the potential target of LINC00210. Reverse transcription-quantitative PCR was used to determine LINC00210 and microRNA (miR)-328-5p expression levels in NSCLC tissues and tumor cell lines (SK-MES-1 and A549). The results demonstrated that LINC00210 was upregulated in NSCLC tissues and cell lines compared with that in normal tissues and 16-HBE cells, and that LINC00210 expression was associated with a poor prognosis in patients with NSCLC (P<0.05). Furthermore, A549 cell transfection with small interfering (si)LINC00210#1 and siLINC00210#2 induced a significant decrease in cell proliferation, and migratory and invasive abilities compared with that in the control groups (P<0.05). In addition, miR-328-5p overexpression was stimulated by knockdown of LINC00210. Furthermore, A549 cells transfected with siLINC00210#1 and miR-328-5p inhibitor exhibited a significant increase in cell proliferation, and migratory and invasive ability compared with that in A549 cells transfected with siLINC00210#1. These findings suggest that LINC00210 may serve as an oncogenic role in NSCLC by sponging miR-328-5p.

Circ_MDM2_000139, Circ_ATF2_001418, Circ_CDC25C_002079, and Circ_BIRC6_001271 Are Involved in the Functions of XAV939 in Non-Small Cell Lung Cancer.

Background: The small molecule inhibitor XAV939 could inhibit the proliferation and promote the apoptosis of non-small cell lung cancer (NSCLC) cells. This study was conducted to identify the key circular RNAs (circRNAs) and microRNAs (miRNAs) in XAV939-treated NSCLC cells. Methods: After grouping, the NCL-H1299 cells in the treatment group were treated by 10 µM XAV939 for 12 h. RNA-sequencing was performed, and then the differentially expressed circRNAs (DE-circRNAs) were analyzed by the edgeR package. Using the clusterprofiler package, enrichment analysis for the hosting genes of the DE-circRNAs was performed. Using Cytoscape software, the miRNA-circRNA regulatory network was built for the disease-associated miRNAs and the DE-circRNAs. The DE-circRNAs that could translate into proteins were predicted using circBank database and IRESfinder tool. Finally, the transcription factor (TF)-circRNA regulatory network was built by Cytoscape software. In addition, A549 and HCC-827 cell treatment with XAV939 were used to verify the relative expression levels of key DE-circRNAs. Results: There were 106 DE-circRNAs (including 61 upregulated circRNAs and 45 downregulated circRNAs) between treatment and control groups. Enrichment analysis for the hosting genes of the DE-circRNAs showed that ATF2 was enriched in the TNF signaling pathway. Disease association analysis indicated that 8 circRNAs (including circ_MDM2_000139, circ_ATF2_001418, circ_CDC25C_002079, and circ_BIRC6_001271) were correlated with NSCLC. In the miRNA-circRNA regulatory network, let-7 family members⟶circ_MDM2_000139, miR-16-5p/miR-134-5p⟶circ_ATF2_001418, miR-133b⟶circ_BIRC6_001271, and miR-221-3p/miR-222-3p⟶circ_CDC25C_002079 regulatory pairs were involved. A total of 47 DE-circRNAs could translate into proteins. Additionally, circ_MDM2_000139 was targeted by the TF POLR2A. The verification test showed that the relative expression levels of circ_MDM2_000139, circ_CDC25C_002079, circ_ATF2_001418, and circ_DICER1_000834 in A549 and HCC-827 cell treatment with XAV939 were downregulated comparing with the control. Conclusions: Let-7 family members and POLR2A targeting circ_MDM2_000139, miR-16-5p/miR-134-5p targeting circ_ATF2_001418, miR-133b targeting circ_BIRC6_001271, and miR-221-3p/miR-222-3p targeting circ_CDC25C_002079 might be related to the mechanism in the treatment of NSCLC by XAV939.

Long noncoding RNA MNX1-AS1 contributes to lung cancer progression through the miR-527/BRF2 pathway.

Lung cancer belongs to a leading popular and malignant cancer around the world. However, the root mechanism underlying lung cancer progression remains unclear. Recently, long noncoding RNA (lncRNA) has been identified as important for tumorigenesis. LncRNA MNX1-AS1 is proven to regulate colon adenocarcinoma, cervical cancer, glioblastoma, and ovarian cancer. Whether MNX1-AS1 participates in lung cancer needs investigation. In our research, we found that MNX1-AS1 was dramatically upregulated in lung cancer. MNX1-AS1 upregulation indicated poor prognosis in lung cancer patients. Functionally, MNX1-AS1 promoted lung cancer progression through regulating proliferation, migration, and invasion. Mechanistically, MNX1-AS1 was found to locate in the cytoplasm and interact with miR-527. Through inhibiting miR-527 availability, MNX1-AS1 facilitated BRF2 expression. Restoration of BRF2 rescued defects of proliferation, migration, and invasion caused by MNX1-AS1 knockdown. Taken together, our study found a novel signaling pathway, namely MNX1-AS1/miR-527/BRF2 axis, involved in lung cancer progression.

Biophysical effect of conversion from croplands to grasslands in water-limited temperate regions of China.

The biophysical effect of land use and land cover change (LUCC) on regional climatic regulation is currently of growing interest. However, in water-limited temperate regions, the net biophysical effect of conversion from croplands to grasslands on regional climatic regulation remains poorly understood to date. To answer this concern, a modified land surface model (mEASS) and two different land use scenarios in a typical study area of the Loess Plateau of China were used in this study. We first validated the performances of mEASS model by using observations from six flux tower sites with different land cover and three metrics of the coefficient of determination (R2), the root mean square error (RMSE) and the difference between the simulated and observed data (bias). Subsequently, the biophysical effect of conversion from croplands to grasslands was investigated. Results indicated that mEASS model could well capture the seasonal dynamics of net radiation and latent heat with high R2 and lower RMSE and bias at grassland, forest and cropland sites. In the context of semi-arid and semi-humid climatic conditions, conversion from croplands to grasslands caused the cooling effect (-0.3 W/m2) at the annual scale. Similar cooling effects were found in spring (-0.4 W/m2), autumn (-0.8 ±â€¯0.1 W/m2) and winter (-0.9 ±â€¯0.1 W/m2). The decreased latent heat (inducing warming effects) were completely offset by decreased net radiation (inducing cooling effects), which were responsible for the net cooling effects. However, a warming effect with 1.0 ±â€¯0.1 W/m2 was observed in summer. This is because that magnitude of decreased latent heat is greater than that of decreased net radiation in summer. These findings will enrich our understanding for the biophysical effect of conversion from croplands to grasslands in water-limited temperate regions.

Early-season crop mapping using improved artificial immune network (IAIN) and Sentinel data.

Substantial efforts have been made to identify crop types by region, but few studies have been able to classify crops in early season, particularly in regions with heterogeneous cropping patterns. This is because image time series with both high spatial and temporal resolution contain a number of irregular time series, which cannot be identified by most existing classifiers. In this study, we firstly proposed an improved artificial immune network (IAIN), and tried to identify major crops in Hengshui, China at early season using IAIN classifier and short image time series. A time series of 15-day composited images was generated from 10 m spatial resolution Sentinel-1 and Sentinel-2 data. Near-infrared (NIR) band and normalized difference vegetation index (NDVI) were selected as optimal bands by pair-wise Jeffries-Matusita distances and Gini importance scores calculated from the random forest algorithm. When using IAIN to identify irregular time series, overall accuracy of winter wheat and summer crops were 99% and 98.55%, respectively. We then used the IAIN classifier and NIR and NDVI time series to identify major crops in the study region. Results showed that winter wheat could be identified 20 days before harvest, as both the producer's accuracy (PA) and user's accuracy (UA) values were higher than 95% when an April 1-May 15 time series was used. The PA and UA of cotton and spring maize were higher than 95% with image time series longer than April 1-August 15. As spring maize and cotton mature in late August and September-October, respectively, these two crops can be accurately mapped 4-6 weeks before harvest. In addition, summer maize could be accurately identified after August 15, more than one month before harvest. This study shows the potential of IAIN classifier for dealing with irregular time series and Sentinel-1 and Sentinel-2 image time series at early-season crop type mapping, which is useful for crop management.

No evidence of widespread decline of snow cover on the Tibetan Plateau over 2000-2015.

Understanding the changes in snow cover is essential for biological and hydrological processes in the Tibetan Plateau (TP) and its surrounding areas. However, the changes in snow cover phenology over the TP have not been well documented. Using Moderate Resolution Imaging Spectroradiometer (MODIS) daily snow products and the Interactive Multi-sensor Snow and Ice Mapping System (IMS) data, we reported daily cloud-free snow cover product over the Tibetan Plateau (TP) for 2000-2015. Snow cover start (SCS), melt (SCM) and duration (SCD) dates were calculated for each hydrological year, and their spatial and temporal variations were analyzed with elevation variations. Our results show no widespread decline in snow cover over the past fifteen years and the trends of snow cover phenology over the TP has high spatial heterogeneity. Later SCS, earlier SCM, and thus decreased SCD mainly occurred in the areas with elevation below 3500 m a.s.l., while regions in central and southwestern edges of the TP showed advanced SCS, delayed SCM and consequently longer SCD. The roles of temperature and precipitation on snow cover penology varied in different elevation zones, and the impact of both temperature and precipitation strengthened as elevation increases.

Comparison of surface energy budgets and feedbacks to microclimate among different land use types in an agro-pastoral ecotone of northern China.

The biophysical effect of land use conversion plays a significant role in regulating climate change. Owing to albedo and evapotranspiration (ET) change, the effect of energy budget difference on land surface temperature (LST) is important but unclear among contrasting land use types, especially in temperate semi-arid regions. Based on moderate-resolution imaging spectroradiometer (MODIS) data, we compared the differences in albedo, ET, and LST between cropland and grassland (CR-GR), and between planted forest and grassland (PF-GR) in the Horqin Sandy Land of Inner Mongolia, an agro-pastoral ecotone of northern China. Our main objective was to explore the magnitude and direction of albedo and ET change during the growing season and, subsequently, to estimate the biophysical effects on LST as a result of land use and land cover change. Our results indicate no significant difference in mean monthly albedo for CR-GR and PF-GR. Cropland lost more water through ET and significantly decreased daytime LST compared with grassland from July to September, but no significant differences in ET and LST were observed for PF-GR in any month. The biophysical climate effects were more pronounced for CR-GR compared with PF-GR. The response of LST to the changes in energy budget confirmed that ET was the critical driving factor relative to albedo. Compared with grassland, cropland and planted forest tended to cool the land surface by 5.15°C and 1.51°C during the growing season, respectively, because of the biophysical effects. Our findings suggest the significance of local-scale biophysical effect on climate variation after land use conversion in semi-arid regions.

[Vulnerability assessment of eco-environment in Yimeng mountainous area of Shandong Province based on SRP conceptual model].

Based on the ecological sensitivity-resilience-pressure (SRP) conceptual model, and selecting 13 indices including landscape diversity index, soil erosion, and elevation, etc. , the vulnerability of the eco-environment in Yimeng mountainous area of Shandong Province was assessed under the support of GIS and by using principal component analysis and hierarchy analytical method. According to the eco-environmental vulnerability index (EVI) values, the eco-environment vulnerability of study area was classified into 5 levels, i.e., slight (<1.8), light (1.8-2.8), moderate (2.8-3.5), heavy (3.5-4.0), and extreme vulnerability (>4.0). In the study area, moderately vulnerable area occupied 43.3% of the total, while the slightly, lightly, heavily, and extremely vulnerable areas occupied 6.1%, 33.8%, 15.9%, and 0.9%, respectively. The heavily and extremely vulnerable areas mainly located in the topographically complicated hilly area or the hill-plain ecotone with frequent human activities.