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1.
Rev Esp Med Nucl ; 22(5): 336-9, 2003.
Artigo em Espanhol | MEDLINE | ID: mdl-14534009

RESUMO

Clear cell sarcoma of the soft parts (CCSSP) is a rare and highly malignant tumor. This is a case report of a 31 years old woman who presented with a tumor in the internal face of left thigh. The tumor biopsy was suggestive of a possible malignant tumor of the peripheral nerve sheath (malignant schwannoma). Biochemical analyses, computed tomography and magnetic resonance were performed and reported a sarcoma of soft parts (CCSSP) without abnormal inguinal lymph nodes. With this diagnostic suspicion, the patient was sent to the Nuclear Medicine Service of our center where a 201Thallium scintigraphy study was performed. In this study, it showed the primary tumor together with a hot spot in the homolateral inguinal region, suggestive of the presence of a metastastic lymph node. This finding would change the surgical attitude in the patient, including inguinal lymphadenectomy. The post-surgical histological study confirmed a clear cell sarcoma of soft parts (CCSSP) with a metastatic inguinal lymph node.


Assuntos
Compostos Radiofarmacêuticos , Sarcoma de Células Claras/diagnóstico por imagem , Neoplasias de Tecidos Moles/diagnóstico por imagem , Tendões/diagnóstico por imagem , Radioisótopos de Tálio , Adulto , Diagnóstico Diferencial , Feminino , Humanos , Metástase Linfática , Imageamento por Ressonância Magnética , Neurilemoma/diagnóstico por imagem , Cintilografia , Sarcoma de Células Claras/diagnóstico , Neoplasias de Tecidos Moles/diagnóstico , Tálio , Coxa da Perna , Tomografia Computadorizada por Raios X
2.
Clin Lab Haematol ; 24(3): 145-50, 2002 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12067277

RESUMO

There are two major forms of the BCR/ABL fusion gene, involving ABL exon 2, but including different exons of BCR gene. The transcripts b2a2 or b3a2 code for a p210 protein. Another fusion gene leads to the expression of an e1a2 transcript, which codes for a p190 protein. Another, less common fusion gene is c3a2[e19a2], which encodes a p230 protein. The incidence of one or the other rearrangement in chronic myeloid leukaemia (CML) patients varies in different reported series. This study was designed to determine the frequency of coexpresion of the p210, p190 and p230 transcripts in 250 Mexican patients with CML. We performed nested and multiplex reverse transcriptase polymerase chain reaction (RT-PCR) on bone marrow samples from adult patients and found that all cases were positive for some type of BCR/ABL rearrangement. In 226 (90.4%) patients it was p210, while the remaining 9.6% showed coexpression or one of the transcripts of p190/p210/p230. In 7% of patients with p210 expression there are both isoforms (b3a2/b2a2), presumably the result of alternative splicing. The rate of coexpression of the p190/p210 transcripts was 5%, which is much lower than in other reports. This may be due to the technical factors. These patients had high platelet counts, marked splenomegaly and chromosomal abnormalities in addition to Ph'. Other types of coexpression seen were p210/p230 and p190/p210/p230, in patients with high-risk clinical factors. Our study confirms the occurrence of coexpression of different BCR/ABL transcripts, although the rate (9.6%) was much lower than has been reported in other populations. This may reflect either the sensitivity of the detection techniques used or the possibility of genetic differences between the populations studied. Coexpression may be due to alternative splicing or to phenotypic variation, with clinical courses different from classical CML.


Assuntos
Proteínas de Fusão bcr-abl/genética , Leucemia Mielogênica Crônica BCR-ABL Positiva/genética , Adolescente , Adulto , Idoso , Análise Citogenética , Éxons , Feminino , Rearranjo Gênico , Variação Genética , Humanos , Leucemia Mielogênica Crônica BCR-ABL Positiva/epidemiologia , Leucemia Mielogênica Crônica BCR-ABL Positiva/patologia , Masculino , México/epidemiologia , Pessoa de Meia-Idade , Fenótipo , Isoformas de Proteínas/análise , Isoformas de Proteínas/genética , RNA Mensageiro/análise , RNA Mensageiro/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa
3.
Poult Sci ; 80(6): 727-34, 2001 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-11441839

RESUMO

N-acetylcysteine (NAC) has been used safely in humans and in other mammals as an antidote against several toxic and carcinogenic agents, including aflatoxin B1 (AFB1). The aim of this study was to evaluate the capability of dietary supplementation with NAC to ameliorate the effects of subacute intoxication with AFB1 in broiler chickens. One hundred twenty male Hubbard 1-d-old chickens were allocated into one of four dietary treatments: 1) control group without treatment, 2) purified AFB1 added to diet (3 mg/kg of feed) for 21 d, 3) NAC (800 mg/kg BW, daily), or 4) AFB1 plus NAC at the same doses as Groups 2 and 3. Broilers treated with AFB1 plus NAC were shown to be partially protected against deleterious effects on BW (57.8%), daily weight gain (49.1%), feed conversion index (21.4%), plasma and hepatic total protein concentration (45.2, 66.7%), plasma alanine aminotransferase (67.4%), hepatic glutathione-S-transferase (18.8%), and reduced glutathione liver concentration (75.0%). In addition, they showed less intense liver fading, friable texture, and microvesicular steatosis. In the kidney, thickening of glomerular basement membrane was also less severe in NAC+AFB1-treated chickens than in AFB1-treated chickens. Our results suggest that NAC provided protection against negative effects on performance, liver and renal damage, and biochemical alterations induced by AFB1 in broiler chickens. Effects of NAC alone on chick performance were also evaluated. Addition of NAC to diet (800 mg/kg BW) did not negatively affect feed consumption, conversion index, or serum chemistry and did not induce structural changes in the liver or kidney.


Assuntos
Acetilcisteína/farmacologia , Aflatoxina B1/antagonistas & inibidores , Galinhas , Sequestradores de Radicais Livres/farmacologia , Doenças das Aves Domésticas/prevenção & controle , Aflatoxina B1/toxicidade , Alanina Transaminase/sangue , Alanina Transaminase/efeitos dos fármacos , Ração Animal , Animais , Aspartato Aminotransferases/sangue , Aspartato Aminotransferases/efeitos dos fármacos , Proteínas Sanguíneas/efeitos dos fármacos , Proteínas Sanguíneas/metabolismo , Peso Corporal/efeitos dos fármacos , Doença Hepática Induzida por Substâncias e Drogas , Glutationa/efeitos dos fármacos , Glutationa/metabolismo , Glutationa Transferase/efeitos dos fármacos , Glutationa Transferase/metabolismo , Rim/efeitos dos fármacos , Rim/patologia , Nefropatias/induzido quimicamente , Nefropatias/veterinária , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Hepatopatias/veterinária , Masculino , Doenças das Aves Domésticas/induzido quimicamente , Distribuição Aleatória , Resultado do Tratamento
4.
J Agric Food Chem ; 49(2): 946-51, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11262054

RESUMO

Flame Seedless grapes were sprayed with N-(2-chloro-4-pyridyl)-N-phenylurea (CPPU) at 0, 2.5, and 5.0 ppm to develop rachis resistant to browning and dehydration. Rachis polyphenol oxidase (PPO) activity was determined during cluster development. Cluster components were weighed at commercial (CM), and physiological maturity (PM). PPO activity, rachis color changes (L and a), and cluster weight loss were evaluated at 0 degrees C for 8, 16, 32, and 56 days. CPPU-treated rachis had a decrease of 36% in PPO activity and a week delay in peak activity. At PM, dry weight of CPPU-treated rachis increased by 3 g. Postharvest rachis PPO activity declined with CPPU application, and color changes followed the same pattern for CM and PM. After 32 days of storage, L and a in lateral branches were significantly superior in CPPU treatments. Weight losses below 2.1% were significantly lowest in CPPU-treated clusters for 16 days of storage regardless of cluster maturity.


Assuntos
Catecol Oxidase/metabolismo , Compostos de Fenilureia/farmacologia , Caules de Planta/enzimologia , Piridinas/farmacologia , Rosales/enzimologia , Catecol Oxidase/efeitos dos fármacos , Cor , Citocininas/farmacologia , Dessecação , Manipulação de Alimentos , Reação de Maillard/efeitos dos fármacos , Fatores de Tempo
5.
Ginecol Obstet Mex ; 68: 97-104, 2000 Mar.
Artigo em Espanhol | MEDLINE | ID: mdl-10808614

RESUMO

Ten healthy patients with term pregnancy resolved by abdominal via. During the surgical procedure samples of umbilical chord serum and maternal adipose tissue, were taken. In all the samples, together with the maternal milk collected the day 10 of puerperium, the concentrations of the following organochlorine pesticides, were measured up: (PCC); beta-BHC; gamma-BHC; heptachloride; aldrin; dieldrin, DDE, DDD, DDT and methoxychloride. The identification and quantification of pesticides was done by the comparison with standards certified by NIST (National Institute of Standard Technology). The general characteristics of the participants were: primigestas of 24.1 years aged, married and of a low socioeconomical level. As to the neonates, of 39 weeks of gestational age, female sex; 3,311 g of corporal weight and size of 51.1 cm. In all the analyzed samples at least one of the organochlorine pesticides was present. The results of correlation analysis between DDT concentration, present in the maternal serum with those identified in the adipose tissue and serum from the umbilical chord were highly significant: a = 0.97 and 0.87, respectively. In the maternal milk the highest concentrations of total DDT, were found, average of 2053 ng/g lipidic base, which is 2.8 times more of daily accepted intake. Likewise, DDT concentration in maternal serum kept exponential relation, growing with age (a = 0.99).


Assuntos
Tecido Adiposo/química , DDT/análise , Sangue Fetal/química , Inseticidas/análise , Leite Humano/química , Adolescente , Adulto , Idoso , Aldrina/análise , Aldrina/sangue , Índice de Apgar , Cromatografia Líquida de Alta Pressão , DDT/sangue , Diclorodifenil Dicloroetileno/análise , Diclorodifenil Dicloroetileno/sangue , Diclorodifenildicloroetano/análise , Diclorodifenildicloroetano/sangue , Dieldrin/análise , Dieldrin/sangue , Feminino , Humanos , Recém-Nascido , Inseticidas/sangue , Masculino , Paridade , Período Pós-Parto , Gravidez , Estudos Prospectivos
6.
Int J Dermatol ; 38(1): 46-51, 1999 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-10065610

RESUMO

BACKGROUND: Pemphigoid gestationis (PG), also called herpes gestationis, is a rare autoimmune disease of pregnancy or puerperium (estimated 1 out of 50,000 pregnancies among Caucasians). A previous series has demonstrated an association of PG with human leukocyte antigen (HLA)-DR3 or HLA-DR4 haplotypes. While these haplotypes are most commonly found in individuals of European ancestry, they have also been found in African-American patients affected with PG. PG has rarely been reported in other ethnic groups, and the HLA association in non-Europeans has not been examined. METHODS: We have characterized eight patients of Mexican ancestry who have PG by clinical, histologic, and immunofluorescence criteria. Class I and class II major histocompatibility complex (MHC) antigens were studied by standard microlymphocytotoxicity assays. Class II MHC antigens were further studied by polymerase chain reaction (PCR) amplification of HLA-DRB1, DQA, and DQB genes and allele-specific oligonucleotide hybridization. For comparison purposes, we used results obtained from a group of 100 ethnically matched healthy individuals. RESULTS: We found that all eight patients had the HLA-DR3/DR4 phenotype; all HLA-DR3 haplotypes were HLA-DRB1*0301, DQA1*0501, and DQB1*0201, whereas half of the HLA-DR4 haplotypes were from the DRB1*0401 subtype and the other half were DRB1 *0407. CONCLUSIONS: These results suggest that, in Mexicans, the genetic susceptibility for the development of PG is strongly influenced by the genetic admixture of Caucasian origin, and the role of class II MHC antigens in the pathophysiology of this disease is confirmed.


Assuntos
Antígenos de Histocompatibilidade Classe II/genética , Penfigoide Gestacional/etnologia , Penfigoide Gestacional/genética , Adolescente , Adulto , Feminino , Técnica Direta de Fluorescência para Anticorpo , Frequência do Gene , Predisposição Genética para Doença , Idade Gestacional , Antígenos de Histocompatibilidade Classe I/genética , Teste de Histocompatibilidade , Humanos , México/etnologia , Penfigoide Gestacional/patologia , Fenótipo , Reação em Cadeia da Polimerase , Gravidez , Pele/patologia
7.
Biol Neonate ; 74(5): 385-92, 1998 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-9742268

RESUMO

Administration of ethanol (8%) or acetone (1%) to nursing dams in the drinking water, for 10 days, increased the nephrotoxicity of paracetamol (APAP) in the 14-day-old lactating offspring. The percentage of proximal tubular cells with evidence of necrotic damage in male rats was higher in those animals that received APAP (500 mg/kg, i.p.) and whose nursing rats were exposed to ethanol (25. 0 +/- 8.4%) or acetone (17.2 +/- 1.2%), than in the group treated with APAP alone (10.6 +/- 1.6%). The activity of urinary N-acetylglucuronidase was also significantly higher in the rats exposed translactationally to ethanol or acetone than in animals treated with the APAP alone. Nephrotoxicity showed a sexual dimorphic pattern with a higher toxicity in male than in female rats. The percentage of necrotic tubules in the male rats not exposed to inductor was 10.6 +/- 1.6%, and in female rats 5.0 +/- 1.4% (p < 0. 05). Animals exposed to ethanol or acetone and treated with APAP showed less weight gain than the group treated only with APAP. Our results suggest that renal toxicity is enhanced in the nursing animals that were exposed, via maternal milk, to ethanol or acetone (inductors of cytochrome P4502E1), than in the control animals. This circumstance may be relevant in alcoholic women while they are lactating.


Assuntos
Acetaminofen/intoxicação , Etanol/administração & dosagem , Rim/efeitos dos fármacos , Lactação/fisiologia , Acetona/administração & dosagem , Acetona/farmacologia , Animais , Animais Lactentes , Sinergismo Farmacológico , Etanol/farmacologia , Feminino , Glucuronidase/urina , Rim/patologia , Masculino , Ratos , Ratos Wistar , Caracteres Sexuais
9.
Acta Otorrinolaringol Esp ; 44(3): 223-6, 1993.
Artigo em Espanhol | MEDLINE | ID: mdl-8395194

RESUMO

The propose of this article is to show a case that presented several mixed tumours in the greater and minor salivary glands during 22 years. The patient was systematically studied according to the world's literature and the result obtained was the diagnosis of pleomorphic adenoma, both in the pre and post operatic phase.


Assuntos
Adenoma Pleomorfo/diagnóstico , Neoplasias das Glândulas Salivares/diagnóstico , Adenoma Pleomorfo/terapia , Adulto , Humanos , Masculino , Neoplasias das Glândulas Salivares/terapia
10.
Actas Urol Esp ; 17(1): 57-61, 1993 Jan.
Artigo em Espanhol | MEDLINE | ID: mdl-8452085

RESUMO

Study of group of 61 patients, nephrectomized as a result of various diseases and who before and three months after surgery underwent blood pressure, effective renal plasma flow (EPFF) and unilateral renal function determinations in order to verify the compensating ability of the remaining kidney. Effective renal plasma flow was determined by a single injection and removal of six serial blood samples with 125-I-Hippuran. Unilateral renal function was determined from the relative uptake of 99mTc-DMSA 24 hours after injection. The patients were divided into four groups according to their overall and unilateral renal function as well as the presence or absence of hypertension. Patients with normal EPFF and symmetrical renal function showed a significantly increase in the function of the remaining kidney after surgery (p < 0.001). Patients with normal or slightly reduced EPFF (< 10%) and highly asymmetrical unilateral function as well as those with decreased EPFF (> 10%) and symmetrical or asymmetrical unilateral renal function did not increased the function of the remaining kidney after nephrectomy, and hypertensive patients whose blood pressure returned to normal values after nephrectomy had a decreased function of the remaining kidney after surgery (< 0.001). It is concluded that it is possible to predict the functional behaviour of the remaining kidney after nephrectomy, and that the compensating ability will basically depend on the previously existing (overall and unilateral) renal function as well as the presence or absence of hypertension.


Assuntos
Hipertrofia/diagnóstico , Testes de Função Renal , Rim/patologia , Espectroscopia de Ressonância Magnética , Nefrectomia , Velocidade do Fluxo Sanguíneo , Feminino , Humanos , Hipertensão Renal/diagnóstico , Hipertrofia/etiologia , Rim/irrigação sanguínea , Rim/fisiopatologia , Masculino , Cuidados Pós-Operatórios
11.
Arch Invest Med (Mex) ; 11(1): 21-9, 1980.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-6930937

RESUMO

Six adult patients with acute myeloblastic leukemia were treated with chemotherapy and immunotherapy. This was done with cultured autologous leukoblasts, treated with neuraminidase of Vibrio cholerae and radiation, and injected six to ten times each eight to ten days between the chemotherapy cycles. Class IgC leukemic anti-leukoblast antibodies which are able to activate the C3 component of the complement system were found in the serum of patients. Five patients have been in complete remission for more than twenty months and the sixth patient for ten months. Antibodies for C3 fixation were not found in the serum of five other patients who were not immunized, nor in five healthy control subjects.


Assuntos
Leucemia Mieloide Aguda/imunologia , Adolescente , Adulto , Antibióticos Antineoplásicos/análise , Complemento C3/análise , Feminino , Humanos , Imunização , Imunoterapia , Leucemia Mieloide Aguda/radioterapia , Leucemia Mieloide Aguda/terapia , Masculino , Neuraminidase/uso terapêutico
13.
Revista Española de Ortodoncia;26(3): 273-280,
em Espanhol | URUGUAIODONTO | ID: odn-12283
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