RESUMO
This is an innovative study to engineer biological filter to evaluate the effect of template surface structure and physiochemical properties that can be used for wide variety of applications in biological, health care as well as environmental protection. Specifically, planar silicon (Si) wafer and arrayed Si nano-tips (SiNT) templates were fabricated and coated with gold for various lengths of time to study the effect of surface charge, surface roughness, and hydrophilicity on biological activity of rat pheochromocytoma cell lines PC12. The initial growth and proliferation of PC12 cells on Si and SiNT templates showed an antipathy for the ultra-sharp SiNTs templates. In contrast, the same cells demonstrated a preferable adherence to and proliferation on planar Si templates, resulting in higher cell densities by three orders of magnitude than those on SiNT templates. It is hypothesized that SiNTs array does generate nano-fluidic effect such that the effective contact region for aqueous solution on SiNTs is lower than that on planar Si templates, thus decreasing adsorbable area for cell viability and survival. Moreover, the effect of the gold coating on cell number density was analyzed in terms of the surface roughness, zeta potential and wetting properties of the templates. It was determined that surface charge, as measured by the zeta potential, strongly correlated with the trend observed in the surface cell density, whereas no such correlation was observed for surface roughness or wetting properties in the ranges of our experiment conditions.
Assuntos
Filtração/instrumentação , Microfluídica/métodos , Nanoestruturas/química , Neurônios/citologia , Animais , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Microscopia de Fluorescência por Excitação Multifotônica , Nanoestruturas/ultraestrutura , Nanotecnologia , Neurônios/efeitos dos fármacos , Neurônios/ultraestrutura , Células PC12 , Ratos , Silício/farmacologia , Eletricidade Estática , Propriedades de SuperfícieRESUMO
Label free optical sensing of adenine and thymine oligonucleotides has been achieved at the sub-picomole level using self assembled silver nanoparticles (AgNPs) decorated gold nanotip (AuNT) arrays. The platform consisting of the AuNTs not only aids in efficient bio-immobilization, but also packs AgNPs in a three dimensional high surface area workspace, assisting in surface enhanced Raman scattering (SERS). The use of sub-10 nm AgNPs with optimum inter-particle distance ensures amplification of the chemically specific Raman signals of the adsorbed adenine, thymine, cytosine and guanine molecules in SERS experiments. High temporal stability of the Raman signals ensured reliable and repeatable DNA detection even after three weeks of ambient desk-top conservation. This facile architecture, being three dimensional and non-lithographic, differs from conventional SERS platforms.
Assuntos
DNA/análise , DNA/genética , Microquímica/instrumentação , Nanopartículas/química , Análise de Sequência com Séries de Oligonucleotídeos/instrumentação , Prata/química , Análise Espectral Raman/instrumentação , Técnicas Biossensoriais/instrumentação , Desenho de Equipamento , Análise de Falha de Equipamento , Nanopartículas/ultraestrutura , Nanotecnologia/instrumentação , Coloração e RotulagemRESUMO
Nature routinely produces nanostructured surfaces with useful properties, such as the self-cleaning lotus leaf, the colour of the butterfly wing, the photoreceptor in brittlestar and the anti-reflection observed in the moth eye. Scientists and engineers have been able to mimic some of these natural structures in the laboratory and in real-world applications. Here, we report a simple aperiodic array of silicon nanotips on a 6-inch wafer with a sub-wavelength structure that can suppress the reflection of light at a range of wavelengths from the ultraviolet, through the visible part of the spectrum, to the terahertz region. Reflection is suppressed for a wide range of angles of incidence and for both s- and p-polarized light. The antireflection properties of the silicon result from changes in the refractive index caused by variations in the height of the silicon nanotips, and can be simulated with models that have been used to explain the low reflection from moth eyes. The improved anti-reflection properties of the surfaces could have applications in renewable energy and electro-optical devices for the military.
Assuntos
Materiais Biomiméticos/química , Cristalização/métodos , Nanoestruturas/química , Nanoestruturas/ultraestrutura , Nanotecnologia/métodos , Fotometria/métodos , Silício/química , Luz , Substâncias Macromoleculares/química , Teste de Materiais , Conformação Molecular , Dispositivos Ópticos , Tamanho da Partícula , Espalhamento de Radiação , Propriedades de SuperfícieRESUMO
The wetting properties of silicon nanotips (SiNTs) are discussed. SiNTs were prepared by single step dry etching of silicon wafers in an electron cyclotron resonance plasma of silane, methane, argon and hydrogen and water contact angles were measured as a function of their aspect ratio (α) and the inter-tip distance. The hydrophilic nature of the SiNTs is tunable with α and the inter-tip distance. Super-hydrophilicity with water contact angles close to 2° was observed with α>12 (length â¼1500 nm). Upon coating a 1500 nm long SiNT with TiO(2), the water contact angle jumped from 2° to â¼140°, demonstrating a switchover from super-hydrophilic to hydrophobic surface properties.