[The standardization of the methods for assessing the immunogenic activity of all of the components of an adsorbed DTP vaccine]. / K voprosu o standartizatsii metodov otsenki immunolennoi aktivnosti vsekh komponentov AKDS-vaktsiny.

The reproducibility of laboratory methods, recommended by WHO and used by manufacturing and control institutions of most countries for the evaluation of the immunogenic potency of the pertussis, diphtheria and tetanus components of adsorbed DPT vaccine in the process of its production, was studied. This immunogenic potency was experimentally studied in comparison with the immunogenic potency of national standard samples from Bulgaria, Czechoslovakia, Hungary, Poland and Russia. The results of this study indicated that the coded commercial lots of adsorbed DPT vaccine corresponded to WHO requirements with respect to the immunogenic potency of all their components. At the same time, according to data provided by different laboratories, the indices of their immunogenic potency varied within rather wide limits. Taking into account the necessity of dosing all components of adsorbed DPT vaccine by immunizing (protective) units, the reliability of the recommended control methods should be enhanced by the maximum standardization of all testing conditions and the organization of periodic workshops on the basis of the leading WHO laboratory with a view to improving the qualification of specialists carrying out the quality control of commercial lots of adsorbed DPT vaccine.

[The vaccination of children with allergic diseases with reduced doses of adsorbed DT-m anatoxin]. / Vaktsinatsiia detei s allergicheskimi zabolevaniiami umen'shennymi dozami ADS-M-anatoksina.

During a remission and drug therapy, 71 children suffering from allergic diseases were vaccinated with DT-M toxoid containing different dose of diphtheria (1, 2.5, 5 LF) and tetanus (1, 2.5, 5 EC) antigens. It has been shown that all the children responded to tetanus antigen by adequate serological tests, whereas as regards diphtheria toxoid, the patients immunized with the least dose of DT-M preparation (1 LF + + 1 EC antigens) produced specific antibodies later and at a lower level. During the postvaccinal period, they also manifested an increase of the content of IgE and exacerbation of the underlying disease in 17.8% of cases. The use of DT-M toxoid with 2.5 LF + EC antigens was shown to hold promise in vaccination of children suffering from allergic diseases.

[The use of pharmacological screening for characterizing the toxicity of pertussis vaccines]. / Ispol'zovanie farmakologicheskogo skrininga dlia kharakteristiki toksichnosti kokliushnykh vaktsin.

The influence of pertussis preparations, introduced by oral and parenteral routes, on the detoxifying function of the liver and the state of the nervous system of the animals was studied by methods used in pharmacology and toxicology. The use of these methods made it possible to find out side effects produced by corpuscular pertussis vaccine, introduced parenterally, on the detoxifying function of the liver and the state of the nervous system of the animals. The negative influence on the nervous system was more pronounced after the injection of the commercial adsorbed diphtheria-pertussis-tetanus vaccine used in this investigation than after the injection of pertussis monovaccine. The oral administration of corpuscular pertussis vaccine exerted no negative influence on the above-mentioned body functions of the animals.

[The immunological effectiveness of small doses of diphtheria and tetanus anatoxins in the revaccination of children with allergy-altered reactivity and adults]. / Immunologicheskaia éffektivnost' malykh doz difteriinogo i stolbniachnogo anatoksinov pri revaktsinatsii detei s allergicheski-izmenennoi reaktivnost'iu i vzroslykh.

The possibility of decreasing the content of antigens in adsorbed diphtheria-tetanus toxoid, used for the booster immunization of children with allergically altered responsiveness and adults, is shown. Diphtheria toxoid in doses of 1-2.5 Lf and tetanus toxoid in doses of 1-2.5 binding units possessed sufficiently high immunological effectiveness in combination with low reactogenicity and good tolerance. In 6-12 months after booster immunization with decreased doses of toxoids 100% of children with allergy of different character and, respectively, 86-94% and 95% of adults have developed protective levels of antibodies to diphtheria and tetanus.

[Use of adsorbed diphtheria-tetanus (DT) toxoid containing different doses of antigens in booster immunizations of children]. / Primenenie ADS-anatoksina, soderzhashchego razlichnye dozy antigenov, pri povtornykh revaktsinatsiiakh detei.

To decrease the antigenic load in booster immunizations of children against diphtheria and tetanus, the immunological effectiveness and reactogenicity of different doses of both antigens (i. e 1 Lf and 1 BU; 2.5 Lf and 2.5 BU; 5 Lf and 5 BU) were studied. The study revealed that all these doses of the preparation were practically nonreactogenic: the total of systemic reactions was 0.9%. The study of the immunological effectiveness of adsorbed DT toxoid with reduced antigen content revealed the advantage of the commercial dose of the preparation (5 Lf of diphtheria antigen and 5 BU of tetanus antigen) over such doses of diphtheria and tetanus antigens as, respectively, 1 Lf and 1 BU; 2.5 Lf and 2.5 BU. Still, this advantage disappeared as early as 6 months after booster immunization. The results of these investigations indicate that booster immunization of children aged 6 years and over may be made with lower doses of adsorbed DT toxoid with reduced antigen content.

A study on the adhesive properties of Cor. diphtheriae and Cor. parvum in the direct haemagglutination reaction.

The adhesive properties of one Cor. parvum and two Cor. diphtheriae strains were investigated by means of the direct haemagglutination reaction (HAR). Using a specifically developed HAR procedure, it was proved beyond doubt that the studied Corynebacteria possess adhesive properties apparently associated with the presence of pili (fimbrias) on their surfaces. In addition, a correlation was found between the agglutinating activity of bacteria and the conditions of culture growth. Based on the reported results, it is suggested that there exists an intraspecies stability of adhesive properties and a possible link between corynebacterial adhesins and reticulostimulin.