The Effect of Menthol Mouth Rinsing and Fluid Temperature on Male Cycling Performance in Thermoneutral Conditions.

PURPOSE: The purpose of this study was to determine the effect of a menthol (MEN) mouth rinse (MR) on cycling time trial (TT) performance in thermoneutral conditions and to explore the impact of fluid temperature (cold water [CW] or thermoneutral water [TNW]) on MEN's effect on performance. METHODS: Twelve trained male cyclists (VO2 peak, 61.4 ± 12.1 mL/kg/min) completed a cycling TT in thermoneutral conditions (21 ± 0.2 °C, 40 ± 0.6% relative humidity) with four different mouth rinses: (1) MEN + CW; (2) MEN + TNW; (3) CW; and (4) TNW. The time to complete the TT and the power output (W) were recorded. The ratings of perceived exertion (RPE, Borg 6-20), thermal sensation (TS), and thermal comfort (TC) were recorded prior to and throughout the TT. The core body temperature (Tc) and heart rate (HR) were recorded throughout. RESULTS: The TT duration was not significantly different between trials (MEN + TNW: 38:11 ± 12:48, MEN + CW: 37:21 ± 13:00, CW: 38:12 ± 13:54, TNW: 36:06 ± 14:12 mins:secs, p < 0.05). The mean trial power output did not significantly differ between conditions (>0.05). The Tc, HR, RPE, TS, and TC were not significantly different between trials (p > 0.05). CONCLUSION: The results suggest that a MEN MR with either CW or TNW does not significantly improve cycling TT performance in trained male cyclists compared to a CW or TNW MR in thermoneutral conditions.

How Cool is That? The Effects of Menthol Mouth Rinsing on Exercise Capacity and Performance: A Systematic Review and Meta-analysis.

BACKGROUND: Menthol (MEN) mouth rinsing (MR) has gained considerable interest in the athletic population for exercise performance; however, the overall magnitude of effect is unknown. OBJECTIVE: The aim of this systematic review and meta-analysis was to determine the efficacy of menthol MEN MR and the impact it has on exercise capacity and performance. METHODS: Three databases were searched with articles screened according to the inclusion/exclusion criteria. Three-level meta-analyses were used to investigate the overall efficacy of MEN MR and the impact it has on exercise capacity and performance. Meta-regressions were then performed with 1) mean VO2peak, 2) MEN swilling duration; 3) the MEN concentration of MR solution, 4) the number of executed swills throughout a single experiment, 5) the use of flavoured sweetened, non-caloric, or non-flavoured neutral solutions as controls, 6) mean environmental temperature at the time of exercise tests, and 7) exercise type as fixed factors to evaluate their influence on the effects of MEN MR. RESULTS: Ten MEN MR studies included sufficient information pertaining to MEN MR and exercise performance and capacity. MR with MEN resulted in no significant change in capacity and performance (SMD = 0.12; 95% CI - 0.08, 0.31; p = 0.23, n = 1, tau21 < 0.0001, tau22 = < 0.0001, I2 = 0%). No significant influence was detected in meta-regressions for VO2peak, (estimate: 0.03; df = 8; 95% CI - 0.03, 0.09; p = 0.27), swilling duration (5 vs. 10 s: 0.00; df = 16; 95% CI - 0.41, 0.41; p = 1.0), MEN concentration (low [0.01%] vs. high [0.1%]: - 0.08; df = 15; 95% CI - 0.49, 0.32; p = 0.67), number of swills (estimate: 0.02; df = 13; 95% CI - 0.05, 0.09; p = 0.56), the use of flavoured sweetener or non-caloric as control (non-flavoured vs. flavoured: 0.12; df = 16; 95% CI - 0.30, 0.55; p = 0.55) or mean room temperature during exercise tests (estimate: 0.01; df = 16; 95% CI - 0.02, 0.04; p = 0.62). CONCLUSION: MEN MR did not significantly improve overall exercise capacity and performance, though those involved in endurance exercise may see benefits.

Reliability of anaerobic and aerobic mobility performance tests used in wheelchair rugby, wheelchair basketball and wheelchair tennis: A systematic review.

BACKGROUND: Understanding the reliability and validity of field-based mobility and performance tests used within the wheelchair sports of basketball (WCB), rugby (WCR) and tennis (WCT) can assist in understanding an athletes' physiological state, training effects, and/or assist with optimising their wheelchair-user interface. PURPOSE: To examine, evaluate and synthesize current aerobic and anaerobic field-based mobility and performance tests used in WCB, WCR and WCT. METHODS: A systematic search was performed according to PRISMA guidelines. Studies were included if they investigated performance tests in WCB, WCR and WCT and reported reliability. RESULTS: Twenty-one studies covering 45 mobility/performance tests were included (anaerobic, n = 35; aerobic, n = 10), with agility and repeated sprints (n = 13) being the most common, followed by linear-sprints (n = 11). Repeated sprint ability (n = 2) and submaximal field-tests (n = 2) were the least frequent. Intra-class correlations among all tests ranged from 0.62 to 0.99, with agility and repeated sprints being 0.65-0.98, followed by values of 0.62-0.99 for linear-sprint, 0.96-0.99 for repeated sprints and 0.85-0.97 for submaximal field-tests. CONCLUSION: The most frequently measured performance tests were anaerobic tests focusing on agility and repeated sprints. Given the low number of aerobic tests in WCB, WCR and WCT, future research should focus on reliable and valid ways to measure and track performance.

Correction: McKinlay et al. Effects of Post-Exercise Whey Protein Consumption on Recovery Indices in Adolescent Swimmers. Int. J. Environ. Res. Public Health 2020, 17, 7761.

The authors of "Effects of Post-Exercise Whey Protein Consumption on Recovery Indices in Adolescent Swimmers" report an error in Table 1 of their article [...].

Metabolic and Performance Responses to a Simulated Routine in Elite Artistic Swimmers.

The purpose of this investigation was to examine the interrelationship between time spent underwater (UW), movement frequency and accompanying blood acid base balance response. Elite artistic swimmers (n = 6) participated in the investigation and were all familiar with the testing procedures. All athletes completed the same choreographed artistic swimming routine. The routine was videoed and the number of movements during each 'lap' of the routine counted. Fingertip capillary blood samples were collected prior to the routine 60 sec post routine for pH, partial pressure of carbon dioxide (pCO2), partial pressure of oxygen (pO2), bicarbonate (HCO3-) and potassium (K+). and lactate (La) concentration (mmol/L). On a separate day an incremental exercise test to exhaustion was performed on a cycle ergometer for determination of maximal oxygen uptake (VO2max). Over half the routine was performed underwater (56 ± 4%). Aside from pCO2 (-1.07 ± 12.29%, p = 0.686), there were significant changes in all variables measured from the BG analysis. VO2peak was significantly correlated to total UW of the routine (r = -0.93; p = 0.007). as well as ∆PO2 r = 0.47 and ∆HCO3 r = 0.51. There was also a significant correlation between total UW and post routine pCO2 (r = 0.86; p = 0.030). There was also a significant correlation between total movements during the routine and post pO2 (r = -0.83; p = 0.044). These data show UW in combination with movement rate during a AS routine imfluence the metabolic response to the exercise. In addition, VO2max represents an important performance variable influencing AS performance.

Characterizing the Thermal Demands and Mobility Performance During International Wheelchair Rugby Competition.

Objective: To determine the thermoregulatory responses and mobility performance of wheelchair rugby (WCR) players during international competition. Methods: Eleven male National Team WCR players volunteered for the study. Testing occurred during a four game series against international competition (temp 24.7 ± 0.7°C, relative humidity 50.1 ± 3.6%), with movement time (MT) and gastrointestinal temperature (Tgi) recorded continuously. Results: The mean maximal Tgi was 38.6 ± 0.6°C (37.9-39.7) and did not significantly differ among Low-Class, Mid-Class, and High-Class athletes (p > 0.05). Moreover, there was a strong and significant relationship between minutes (min) played per quarter of the game and change in Tgi (r = 0.36, p = 0.01). Athletes moved a total of 27:43 ± 9:40 min:seconds (s), spent a total of 15:02 ± 8.23 min:s in Zone 1 (53.5%), 8:19 ± 3:20 min:s in Zone 2 (31.7%), and 5:59 ± 1:51 min:s in Zone 3 (21.3%). There were no differences among classification in total movement time (p = 0.169) or for speed in Zone 1, Zone 2, or Zone 3 (p > 0.05). The relationship between peak forward speed and total movement time was strong (p = 0.021, r = 0.68). Conclusion: This study demonstrated that the time spent in absolute movement zones is not classification dependent, the change in core temperature is related to movement time per quarter. Furthermore, peak speeds obtained on-court were linked to overall movement time which suggests athletes should warm-up before going on court.

Acute Physiological Response to Different Sprint Training Protocols in Normobaric Hypoxia.

BACKGROUND: the purpose of this study was to examine acute physiological responses to and the performance effects of two sprint training protocols in normobaric hypoxic conditions. METHODS: Healthy competitive female (n = 2) and male (n = 5) kayakers (19 ± 2.1 years) performed four sprint training sessions on a kayak ergometer over a period of two weeks. Participants performed five sets of 12 × 5 s sprints or 3 × 20 s sprints in both normobaric normoxic (NOR, FiO2 = 20.9%) or normobaric hypoxic (HYP, FiO2 = 13.6%) conditions. The peak power output (PPO), rate of perceived exertion (RPE), and heart rate (HR) of each participant were monitored continuously. Their blood lactate concentrations ([BLa+]), in addition to their blood gas (mixed-venous partial pressure (p) of carbon dioxide (pCO2), O2 (pO2), and oxygen saturations (sO2)) were collected before and after exercise. RESULTS: A significantly greater RPE, HR, and [BLa+] response and a significant decrease in pCO2, pO2, and sO2 were observed in HYP conditions versus NOR ones, independent of the type of training session. The PPO of participants did not differ between sessions. Their RPE in HYP12 × 5 was greater compared to all other sessions. CONCLUSIONS: The HYP conditions elicited significantly greater physiological strain compared to NOR conditions and this was similar in both training sessions. Our results suggest that either sprint training protocol in HYP conditions may induce more positive training adaptations compared to sprint training in NOR conditions.

Menthol Mouth Rinsing Is More Than Just a Mouth Wash-Swilling of Menthol to Improve Physiological Performance.

Interventions that solely act on the central nervous system (CNS) are gaining considerable interest, particularly products consumed through the oral cavity. The oropharyngeal cavity contains a wide array of receptors that respond to sweet, bitter, and cold tastants, all of which have been shown to improve physiological performance. Of late, the ergogenic benefits of carbohydrate (CHO) and caffeine (CAF) mouth rinsings (MRs) have been widely studied; however, less is known about menthol (MEN). That the physiological state and environmental conditions impact the response each product has is increasingly recognized. While the effects of CHO and CAF MRs have been thoroughly studied in both hot and thermoneutral conditions, less is known about MEN as it has only been studied in hot environments. As such, this review summarizes the current knowledge regarding the MEN MR and exercise modality, frequency of the mouth rinse, and mouth rinse duration and compares two different types of study designs: time trials vs. time to exhaustion (TTE).

Applicability of Maximal Ergometer Testing and Sprint Performance in Adolescent Endurance and Sprint Trained Swimmers.

Sprint swimming is a short duration, high intensity sport requiring a relatively greater contribution of energy from anaerobic metabolism. Understanding energy system utilization for the classification of a competitive swimmer (sprint or distance) may be useful for both training prescription and event specialization. The relationship between anaerobic swim ergometer testing and adolescent sprint swimming performance has not been investigated. The purpose of this study was to compare the performance and physiological responses during a maximal all-out ergometer test as well as the maximal anaerobic lactate test in a group of sprint vs. middle-distance specialized swimmers. Sixteen (n = 16) competitive swimmers (mean ± standard deviation (SD), age 16.8 ± 0.7 year; body mass 67.3 ± 9.8 kg) were categorized into two gender matched groups: sprint (n = 8) and middle-distance (n = 8). Each athlete performed (1) a 45 s swim ergometer maximal test to determine peak and mean power output (Watts (W)), (2) a MANLT test to determine peak and average velocity as well as the post-exercise lactate response, and (3) a 50 m swim time trial. The sprint group showed a higher mean (p = 0.026) and peak (p = 0.031) velocity during the MANLT. In addition, blood lactate concentration was significantly (p < 0.01) higher in the sprint vs. middle-distance trained group at 3 and 12 min after completion of the MANLT (3-min post 11.29 ± 2.32 vs. 9.55 ± 3.48 mmol/L; 12-min post 8.23 ± 2.28 vs. 7.05 ± 2.47 mmol/L). The power output during the 45 s all-out swimming ergometer test was higher in the sprint trained group. The results of this study demonstrate the anaerobic contribution to sprint swimming measured during an all-out dryland ergometer test.

Menthol Mouth Rinsing and Cycling Performance in Females Under Heat Stress.

PURPOSE: The effects of menthol (MEN) mouth rinse (MR) on performance, physiological, and perceptual variables in female cyclists during a 30-km independent time trial (ITT) were tested. METHODS: The participants (n = 9) cycled for 30 km in hot conditions (30°C [0.6°C], 70% [1%] relative humidity, 12 [1] km/h wind speed) on 2 test occasions: with a placebo MR and with MEN MR. Handgrip and a 5-second sprint were measured before, following the first MR, and after the ITT. Ratings of perceived exertion Borg 6 to 20, thermal sensation, and thermal pleasantness were recorded every 5 km. Core temperature and heart rate were recorded throughout. RESULTS: The ITT performance significantly improved with MEN MR by 2.3% (2.7%) relative to the placebo (62.6 [5.7] vs 64.0 [4.9] min P = .034; d = 0.85; 95% confidence interval, 0.14 to 2.8 min). The average power output was significantly higher in the MEN trial (P = .031; d = 0.87; 95% confidence interval, 0.9 to 15.0 W). No significant interaction of time and MR for handgrip (P = .581, η2 = .04) or sprint was observed (P = .365, η2 = .103). Core temperature, heart rate, ratings of perceived exertion, and thermal sensation did not significantly differ between trials at set distances (P > .05). Pleasantness significantly differed between the placebo and MEN only at 5 km, with no differences at other TT distances. CONCLUSION: These results suggest that a nonthermal cooling agent can improve 30-km ITT performance in female cyclists, although the improved performance with MEN MR is not due to altered thermal perception.

The Effects of Anaerobic Swim Ergometer Training on Sprint Performance in Adolescent Swimmers.

PURPOSE: The purpose of this study was to compare 4 weeks of pool-based sprint interval training with a similar ergometer training intervention on a maximal anaerobic lactate test (MANLT), 50-m (competition) freestyle performance, and 6- and 30-second maximal swimming ergometer performances. METHODS: A total of 14 competitive adolescent swimmers (male, n = 8; female, n = 6) participated in this study. Swimmers were categorized into 2 sex-matched groups: swimming ergometer (ERG; n = 7) and pool-sprint training (n = 7) groups. Each athlete performed 4 preintervention and postintervention assessments consisting of a MANLT, a 50-m freestyle race, and 6- and 30-second maximal swim ERG performances. RESULTS: Both groups demonstrated a significant effect (P < .05) of time for all assessments. Group differences were observed after 4 weeks of sprint interval training as follows: (1) The ERG group had a significantly faster speed in the fourth 50-m MANLT sprint (ERG 1.58 [0.05] vs pool-sprint training 1.48 [0.07] m/s, P < .01) and (2) The ERG group demonstrated enhanced Δblood lactate post-MANLT (ERG 2.4 [1.2] vs pool-sprint training 2.7 [0.9] mmol/L, P < .05). A significant correlation was found between the 30-second maximal ERG test and 50-m freestyle swimming velocity (r = .74, P < .01, effect size = 0.52). CONCLUSIONS: The results demonstrate significant physiological improvements to anaerobic sprint ability after 4 weeks of sprint interval training in both swim ERG and pool-based interventions. Thus, sprint ability may be improved through multiple modalities (pool and dry land) to elicit a positive training response.

Physiological Considerations to Support Podium Performance in Para-Athletes.

The twenty-first century has seen an increase in para-sport participation and the number of research publications on para-sport and the para-athlete. Unfortunately, the majority of publications are case reports/case series or study single impairment types in isolation. Indeed, an overview of how each International Paralympic Committee classifiable impairment type impact athlete physiology, health, and performance has not been forthcoming in the literature. This can make it challenging for practitioners to appropriately support para-athletes and implement evidence-based research in their daily practice. Moreover, the lack of a cohesive publication that reviews all classifiable impairment types through a physiological lens can make it challenging for researchers new to the field to gain an understanding of unique physiological challenges facing para-athletes and to appreciate the nuances of how various impairment types differentially impact para-athlete physiology. As such, the purpose of this review is to (1) summarize how International Paralympic Committee classifiable impairments alter the normal physiological responses to exercise; (2) provide an overview of "quick win" physiological interventions targeted toward specific para-athlete populations; (3) discuss unique practical considerations for the para-sport practitioner; (4) discuss research gaps and highlight areas for future research and innovation, and (5) provide suggestions for knowledge translation and knowledge sharing strategies to advance the field of para-sport research and its application by para-sport practitioners.

Relationship between VO 2max , under Water Swim Testing and Artistic Swim Solo Performance.

The purpose of this study was to evaluate the relationship between: 1) laboratory-determined cycling peak oxygen consumption (VO 2max ) and AS performance in a new underwater swim test (UWST), and 2) cycling VO 2max and ventilatory threshold (VT) in cycling and performance score during a simulated AS solo routine. Trained artistic swimmers (n=15, 15.8±0.8 yrs., height: 169.1±5.4 cm, body mass: 57.1±6.3 kg) completed (1) a maximal incremental cycle test to exhaustion to determine VO 2max , (2) the UWST which comprised 275 m of freestyle and underwater breaststroke, and (3) a simulated solo competition where artistic swimming elements were evaluated by five FINA judges. There was a significant correlation between mean element score and (i) VO 2max (48±4 mL . kg . min -1 , r=0.44, p =0.05), and (ii) UWST (r=-0.64, p =0.005). However, there was an insignificant relationship between cycling ventilatory threshold and mean element score (r=-0.36, p =0.10). In addition, the results demonstrate a significant relationship between HR at the ventilatory threshold and peak HR of the UWST (r=-0.64, p =0.014). The results of this study demonstrate that VO 2max is an important determinant of AS performance. In addition, the UWST appears to be a useful indicator of AS performance.

Characterizing thermoregulatory demands of female wheelchair basketball players during competition.

This study aimed to assess the thermoregulatory and cardiovascular responses of WC basketball players during international competitions. Eleven female (7 spinal cord injured (SCI) and 4 Non-SCI) National team WC basketball players volunteered for the study. Testing occurred during a four-game series against the same international competitor (temp 22.1 ± 1.2°C, relative humidity (RH) 55 ± 2%). Hydration habits were monitored and heart rate, skin (SkT) and gastrointestinal (Tc), were recorded. Athletes arrived hydrated for all games (urine specific gravity, 1.014 ± 0.002). Players lost a mean of 0.5 ± 0.1% body mass due to sweat loss and replaced ~69% of fluid losses. SCI athletes played 21 ± 4 min while Non-SCI played 14 ± 6 min. SCI athletes had a mean SkT throughout the game of 35.2 ± 0.2°C and Non-SCI of 36.2 ± 0.2°C. SCI SkT rose a mean of 6.3 ± 1.1°C and Non-SCI 6.8 ± 0.9°C. SCI Tc rose a mean of 1.0 ± 0.2°C and Non-SCI a mean of 0.9 ± 0.4°C. 2/10 players reached a Tc>39°C (SCI athlete class 1, 39.4°C; Non-SCI class 4.5 athlete, 39.5°C). There were differences between players in Tc responses, due to variability in playing time. Monitoring Tc during competition is encouraged to understand competition specific responses and identify athletes at risk of heat-related fatigue.

A Physiological Overview of the Demands, Characteristics, and Adaptations of Highly Trained Artistic Swimmers: a Literature Review.

Artistic swimming (AS) is a very unique sport consisting of difficult artistically choreographed routines ranging in the number of athletes (one to ten: solo, duet, team, combination, highlight routine) and with elements performed quickly and precisely above, below, and on the surface of the water. As a result, the physical and physiological demands placed on an athlete are unique to the sport with the most pronounced adaptation being the bradycardic response to long apneic periods spent underwater while performing strenuous movements. This indeed influences training prescription and the desired training outcomes. This review paper explores the physiological demands of AS, the physiological characteristics that influence AS performance, and innovative approaches to enhancing training and performance in elite performers.

Fluid balance and thermoregulatory responses of competitive triathletes.

As little as 2% total body mass (BM) loss from sweat has been shown to compromise physiological functioning during prolonged exercise in the heat, subsequently compromising endurance performance. PURPOSE: This observational study aims to describe the fluid balance and thermoregulatory responses of competitive triathletes racing at a major international competition in a cool environment. METHODS: Fluid balance and thermoregulatory responses was measured in six (3 male, 3 female) national-level triathletes competing at the ITU World Triathlon Grand Finale in ambient temperatures of 19-20 °C (relative humidity (RH) ~55%). Dry, nude BM was recorded before and immediately following the race. Fluid intake was monitored throughout the race. Pre-race urine samples were measured for specific gravity (USG). Each athlete ingested a core temperature (Tc) pill 5 h prior to the event and was monitored before and after the race. RESULTS: Three of six triathletes arrived at the race mildly dehydrated (USG 1.021,1.024,1.030). One of these athletes (1F) subsequently withdrew from the race providing no further data. Another athlete (1M) ended the race vomiting providing invalid hydration data. The four remaining competitors' sweat loss was on average 2.15 L (range: 1.65-2.80 L), while fluid intake was 0.66 L (0.50-0.85 L). A mean loss of 3.3% (2.2-4.5%) BM was recorded. Tc increased by 2.0 °C (1.1-2.9 °C) and 4/5 athletes' (2 M, 2 F) Tc exceeded 39 °C by race-end. Both female athletes self-reported feelings of heat-related exhaustion at the completion of the race. CONCLUSIONS: Despite cool environmental conditions, elite triathletes lost ~3.3% BM, replacing only 33% of sweat losses, and achieved a Tc > 39 °C by race-end.

The effect of dehydration on muscle metabolism and time trial performance during prolonged cycling in males.

This study combined overnight fluid restriction with lack of fluid intake during prolonged cycling to determine the effects of dehydration on substrate oxidation, skeletal muscle metabolism, heat shock protein 72 (Hsp72) response, and time trial (TT) performance. Nine males cycled at ~65% VO2peak for 90 min followed by a TT (6 kJ/kg BM) either with fluid (HYD) or without fluid (DEH). Blood samples were taken every 20 min and muscle biopsies were taken at 0, 45, and 90 min of exercise and after the TT. DEH subjects started the trial with a -0.6% BM from overnight fluid restriction and were dehydrated by 1.4% after 45 min, 2.3% after 90 min of exercise, and 3.1% BM after the TT. There were no significant differences in oxygen uptake, carbon dioxide production, or total sweat loss between the trials. However, physiological parameters (heart rate [HR], rate of perceived exertion, core temperature [Tc], plasma osmolality [Posm], plasma volume [Pvol] loss, and Hsp72), and carbohydrate (CHO) oxidation and muscle glycogen use were greater during 90 min of moderate cycling when subjects progressed from 0.6% to 2.3% dehydration. TT performance was 13% slower when subjects began 2.3% and ended 3.1% dehydrated. Throughout the TT, Tc, Posm, blood and muscle lactate [La], and serum Hsp72 were higher, even while working at a lower power output (PO). The accelerated muscle glycogen use during 90 min of moderate intensity exercise with DEH did not affect subsequent TT performance, rather augmented Tc, RPE and the additional physiological factors were more important in slowing performance when dehydrated.

The acute effects of fluid intake on urine specific gravity and fluid retention in a mildly dehydrated state.

Many athletes arrive at training sessions and competitions in a mildly hypohydrated (HYPO) state and are instructed to drink fluids before exercise to reach a euhydrated (HYD) state. Ten recreational athletes (6 women, 4 men; 71.9 ± 4.6 kg, 25.2 ± 0.9 years) participated in the studies to examine (a) the day-to-day variability of morning urine specific gravity (USG), (b) the effects of consuming 600 ml of water on the hydration status of HYD and HYPO (USG > 1.020) subjects, and (c) the effects of consuming water (W), salt-water (SW, 40 mM Na), a carbohydrate-electrolyte solution with 3% or light carbohydrate (CES-L, 20 mM Na) or a CES with 6% carbohydrate (CES, 20 mM Na) on the hydration status of HYPO subjects. The hydration status was assessed with USG and body mass measures and urine volume collections. The day-to-day variability in morning USG (coefficient of variation = 0.2 ± 0.1%) was low and the responses to 600 ml of W ingestion were repeatable. Pretrial USG was 1.022 ± 0.001 in the HYPO trial and decreased <1.020 by 45 minutes (1.013 ± 0.003). In the CES study, HYPO subjects reached HYD status at 45 minutes in all conditions (W 1.013 ± 0.003, SW 1.013 ± 0.003, CES-L 1.011 ± 0.003, CES 1.017 ± 0.004) because salt or CES ingestion did not affect fluid retention (W 68%, SW 72%, CES-L 68%, CES 76%). This study demonstrated that mildly HYPO subjects could reach euhydration within 45 minutes of the ingestion of 600 ml of W or a combination of salt and CES solutions. Following this practice will minimize the incidence of starting a practice or competition hypohydrated.

Increase in skeletal-muscle glycogenolysis and perceived exertion with progressive dehydration during cycling in hydrated men.

This study investigated the effects of progressive mild dehydration during cycling on whole-body substrate oxidation and skeletal-muscle metabolism in recreationally active men. Subjects (N = 9) cycled for 120 min at ~65% peak oxygen uptake (VO2peak 22.7 °C, 32% relative humidity) with water to replace sweat losses (HYD) or without fluid (DEH). Blood samples were taken at rest and every 20 min, and muscle biopsies were taken at rest and at 40, 80, and 120 min of exercise. Subjects lost 0.8%, 1.8%, and 2.7% body mass (BM) after 40, 80, and 120 min of cycling in the DEH trial while sweat loss was not significantly different between trials. Heart rate was greater in the DEH trial from 60 to 120 min, and core temperature was greater from 75 to 120 min. Rating of perceived exertion was higher in the DEH trial from 30 to 120 min. There were no differences in VO2, respiratory-exchange ratio, total carbohydrate (CHO) oxidation (HYD 312 ± 9 vs. DEH 307 ± 10 g), or sweat rate between trials. Blood lactate was significantly greater in the DEH trial from 20 to 120 min with no difference in plasma free fatty acids or epinephrine. Glycogenolysis was significantly greater (24%) over the entire DEH vs. HYD trial (433 ± 44 vs. 349 ± 27 mmol · kg-1 · dm-1). In conclusion, dehydration of <2% BM elevated physiological parameters and perceived exertion, as well as muscle glycogenolysis, during exercise without affecting whole-body CHO oxidation.

Effects of dehydration during cycling on skeletal muscle metabolism in females.

INTRODUCTION: This study investigated the effects of progressive dehydration on the time course of changes to whole body substrate oxidation and skeletal muscle metabolism during 120 min of cycling in hydrated females. METHODS: Subjects (n = 9) cycled for 120 min at approximately 65% VO(2peak) on two occasions: with no fluid (DEH) and with fluid (HYD) replacement to match sweat losses. Venous blood samples were taken at rest and every 20 min and muscle biopsies taken at 0, 60, and 120 min of exercise. RESULTS: DEH subjects lost 0.9% body mass from 0 to 60 min and 1.1% from 60 to 120 min (2.0% total). HR and core temperature (Tc) were significantly greater from 30 to 120 min, plasma volume (Pvol) loss from 40 to 120 min, and RPE from 60 to 120 min in the DEH trial. There were no differences in VO(2) or sweat loss between trials. RER (HYD, 0.85 ± 0.01, vs. DEH, 0.87 ± 0.01) and total CHO oxidation (175 ± 17 vs. 191 ± 17 g) were higher in the DEH trial. Blood (La) was significantly higher in the DEH trial, with no change in plasma free fatty acid and epinephrine concentrations. Muscle glycogenolysis was 31% greater in the DEH trial (252 ± 49 vs. 330 ± 33 mmol.kg(-1) dry muscle), and muscle (La) was also higher at 60 min. CONCLUSION: Progressive dehydration significantly increased HR, Tc, RPE, Pvol loss, whole body CHO oxidation, and muscle glycogenolysis, and these changes were already apparent in the first hour of exercise when body mass losses were ≤ 1%. The increased muscle glycogenolysis with DEH appeared to be due to increased core and muscle temperature, secondary to less efficient movement of heat from the core to the periphery.