RESUMO
Among 55 Butyrivibrio fibrisolvens strains five ribotypes of B. fibrisolvens were described on the basis of RFLP profiles of 16S rDNA regions obtained with restriction endonuclease HaeIII. In the phylogenetic tree, these ribotypes were located in the XIVa cluster of Gram-positive bacteria. Phenotypic differences of selected ribotype groups became the basis for further reclassification of B. fibrisolvens.
Assuntos
Sistema Digestório/microbiologia , Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/classificação , Filogenia , Ribotipagem , Rúmen/microbiologia , Anaerobiose , Animais , Butiratos/metabolismo , Meios de Cultura , DNA Bacteriano/genética , DNA Ribossômico/genética , Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/genética , Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/crescimento & desenvolvimento , Humanos , Fenótipo , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , RNA Ribossômico 16S/genéticaRESUMO
Data for bacterial identification were provided by culturing anaerobic bacteria under standardized conditions followed by extraction and methylation of cellular long-chain fatty acids and gas chromatographic analysis. The databases of fatty acid methyl ester (FAMEs) profiles for two predominant ruminal genera, Prevotella and Butyrivibrio, were created. Major long-chain cellular fatty acids found in the 23 analyzed Prevotella strains were 15:0 (anteiso), 15:0, 15:0 (iso) and 16:0. The strains of Prevotella could be well identified on species level by the characteristic ratios among major fatty acids and by acids unique fatty for each species. The 45 Butyrivibrio strains were grouped into 4 major and 2 minor groups according to FAMEs profiles. The major fatty acids for the bulk of the Butyrivibrio strains were 14:0, 15:1, 16:0 and 16:0 (iso). This groups corresponded to those based on 16S rDNA sequences.
Assuntos
Técnicas de Tipagem Bacteriana , Ácidos Graxos/análise , Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/classificação , Prevotella/classificação , Rúmen/microbiologia , Animais , Bases de Dados Factuais , Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos/química , Prevotella/químicaRESUMO
Butyrivibrio sp. Mz 5 with a high xylanolytic activity was isolated. Four major xylanases were detected in the cell-associated fraction using the zymogram technique. The xylanolytic activity was inducible with the oat spelts xylan; two endoxylanases (51 and 145 kDa) were formed constitutively. The bulk of the xylanolytic activity was cell-bound and growth-phase dependent; the maximum activity in the cell-associated fraction was achieved after 16 h of incubation. The highest xylanolytic activity was determined in a medium with 0.5% oat spelts xylan. Under optimum conditions (the highest xylanolytic activity produced), the two cell-bound xylanases (51 and 58 kDa) were isolated by anion exchange chromatography on CIM DEAE 8 tubes attached to a MPLC system, and gel filtration.