[Effects of Equal Amounts Silicon Fertilizer Application on Soil Bioavailability of Cadmium and Cadmium Uptake by Rice].

Cadmium (Cd) contamination of paddy fields is a global concern, as it can cause the accumulation of Cd in food. To explore the effects of equal application of silicon fertilizers on the bioavailability of cadmium and soil Cd uptake at different growth stages of rice, a field experiment was conducted with five silicon fertilizers under the same silicon dose (225 kg·hm-2). The results revealed that the Cd contents in roots, stems, and leaves increased with the extension of the rice growth stage. The application of silicon fertilizers reduced the Cd contents in roots, stems, and leaves in brown rice by 14.9%, 28.2%, and 12.2%, respectively. Compared with that in the control, the Cd content of brown rice in the SiCaMgFe and SiW treatments was decreased by 21.1% (P<0.05) and 21.2% (P<0.05), respectively. Similarly, Cd content in iron plaque (DCB-Cd) increased with the extension of the rice growth period, which accounted for 15.8%-42.8% of the total Cd content in roots, and the DCB-Cd content was different in each stage of rice. The content of exchangeable Cd (Exc-Cd) in soil at the mature stage of rice decreased by 36.4%, and the other fractions increased by 12.5%-48.2%. The results showed significant negative correlations between the Cd contents and Si in roots, DCB-Cd and soil available Cd and available Si, Exc-Cd and Car-Cd, and soil available Cd and pH value. Cd content in roots was positively correlated with DCB-Cd. With the equal dose of silicon fertilizer, the treatments of SiCaMgFe and SiW could effectively reduce the Cd content in rice. The application of silicon fertilizer promoted the transfer of Exc-Cd to Carb-Cd by increasing the soil pH value and the soil available Si content, meanwhile reducing the soil available Cd, Exc-Cd contents, the adsorption of Cd by the iron film on the root surface, and the adsorption capacity of iron plaque and root, thereby reducing the absorption of Cd by rice.

Shifts of lipid metabolites help decode immobilization of soil cadmium under reductive soil disinfestation.

Cadmium (Cd) contamination in soil can cause serious environmental problems and threaten human health. Previous studies have shown that the reductive soil disinfestation (RSD) is regarded as an effective soil disinfection technology, which will affect the bioavailability of Cd. However, the influence of soil microorganisms and their metabolites on the morphologies of Cd during RSD treatment are still poorly understood. Here, a laboratory incubation experiment that composed of untreated soil (CK), two RSD treatments with flooded soil (FL) and added 2% bean dregs soil (BD) was conducted. After the treatment, the content of different morphologies of Cd in the soil and the molecular characteristics (the composition of the microbial community, functional enzymes and metabolites) of the soil were measured. The study found that, compared to CK treatment, the dominant phyla, such as Acidobacteria, Bacteroidetes, Firmicutes, etc., were significantly increased in BD treatment, and enzymes related to metabolism also showed noticeable enhancement. The differential accumulated metabolite (DAM) analysis revealed that the abound of lipids and lipid-like molecules involved with fatty acyls, steroids and steroid derivatives, glycerophospholipids, fatty acids and conjugates, glycerolipids, and sphingolipids were significant different among treatments. The correlation analysis showed the exchangeable fraction cadmium contents (EX-Cd) were negatively correlated with the content of glycerophospholipids and sphingolipids, and positively correlated with glycerolipids content. However, the relationship between the residual cadmium (RS-Cd) and these three metabolites was just the opposite. Compared with another two treatments, the BD treatment significantly reduced EX-Cd contents. Biological interaction network analysis indicated that the phyla Gemmatimonadetes and Proteobacteria assumed the primary responsibility for the morphological transformation of Cd through their corresponding functional enzymes. Overall, this study provided a new perspective on RSD-mediated soil Cd immobilization, and the findings should be beneficial to further applications of RSD technology on the remediation of Cd-polluted soils.

Weak electrical stimulation on biological denitrification: Insights from the denitrifying enzymes.

In order to improve the denitrification efficiency of low carbon to nitrogen ratio (C/N) wastewater, we conducted continuous flow experiments of weakly electrically stimulated denitrification using a direct current output voltage. The results showed that the best denitrification was achieved at a voltage of 0.2 V. The removal of nitrate and total nitrogen was increased by 20% and the production of intermediate greenhouse gas (N2O) was reduced by 62.6%. We explored the specific pathways involved in the weak electrical stimulated denitrification using enzyme activity as a cut-off point. The enzyme activity analysis and 3D fluorescence spectroscopy revealed that nitrate reductase (NAR) and nitrite reductase (NIR) activities were significantly enhanced by weak electrical stimulation, and the aromatic protein content in extracellular polymers substances (EPS) increased, accelerating electron transfer and promoting the conversion of loosely bound EPS (LB) to tightly bound EPS (TB). The accelerated electron transfer further increased enzyme activity and the metabolic rate of microorganisms. This study indicates that weak electrical stimulation could improve activities of biological enzymes to enhance denitrification efficiency.

[Enhancement of artemisinin biosynthesis in transgenic Artemisia annua L. by overexpressed HDR and ADS genes].

Artemisnin is a novel sesquiterpene lactone with an internal peroxide bridge structure, which is extracted from traditional Chinese herb Artemisia annua L. (Qinghao). Recommended by World Health Organization, artemisinin is the first-line drug in the treatment of encephalic and chloroquine-resistant malaria. In the present study, transgenic A. annua plants were developed by overexpressing the key enzymes involved in the biosynthetic pathway of artemisinin. Based on Agrobacterium-mediated transformation methods, transgenic plants of A. annua with overexpression of both HDR and ADS were obtained through hygromycin screening. The genomic PCR analysis confirmed six transgenic lines in which both HDR and ADS were integrated into genome. The gene expression analysis given by real-time quantitative PCR showed that all the transgenic lines had higher expression levels of HDR and ADS than the non-transgenic control (except ah3 in which the expression level of ADS showed no significant difference compared with control); and the HPLC analysis of artemisinin demonstrated that transgenic A. annua plants produced artemisinin at significantly higher level than non-transgenic plants. Especially, the highest content of artemisinin was found in transgenic line ah70, in which the artemisinin content was 3.48 times compared with that in non-transgenic lines. In summary, overexpression of HDR and ADS facilitated artemisinin biosynthesis and this method could be applied to develop transgenic plants of A. annua with higher yield of artemisinin.

[Enhancement of tropane alkaloids production in transgenic hair roots of Atropa belladonna by overexpressing endogenous genes AbPMT and AbH6H].

Atropa belladonna L. is the officially medicinal plant species and the main commercial source of scopolamine and hyoscyamine in China. In this study, we reported the simultaneous overexpression of two functional genes involved in biosynthesis of scopolamine, which respectively encoded the upstream key enzyme putrescine N-methyltransferase (PMT; EC 2.1.1.53) and the downstream key enzyme hyoscyamine 6beta-hydroxylase (H6H; EC 1.14.11.11) in transgenic hair root cultures of Atropa belladonna L. HPLC results suggested that four transgenic hair root lines produced higher content of scopolamine at different levels compared with nontransgenic hair root cultures. And scopolamine content increased to 8.2 fold in transgenic line PH2 compared with that of control line; and the other four transgenic lines showed an increase of scopolamine compared with the control. Two of the transgenic hair root lines produced higher levels of tropane alkaloids, and the content increased to 2.7 fold in transgenic line PH2 compared with the control. The gene expression profile indicated that both PMT and H6H expressed at a different levels in different transgenic hair root lines, which would be helpful for biosynthesis of scopolamine. Our studies suggested that overexpression of A. belladonna endogenous genes PMT and H6H could enhance tropane alkaloid biosynthesis.