Bacteriemia por Leuconostoc spp: Descripción de un caso / A case of bacteremia caused by Leuconostoc spp

Leuconostoc spp es una bacteria tipo coco gram-positivo; recientemente se ha demostrado su potencial patógeno, sobre todo en pacientes inmunodeprimidos y con factores de riesgo como la patología oncológica. Dentro de los cuadros clínicos que pueden producir destacan: neumonía, meningitis, endocarditis o bacteriemia, siendo estas últimas las más frecuentes. Cabe destacar su resistencia intrínseca al tratamiento con vancomicina, siendo el tratamiento de elección en estos casos la penicilina y otros fármacos pertenecientes al grupo de los beta-lactámicos. (AU)

Leuconostoc spp is a gram-positive bacterium which pathogenic potential has been recently demonstrated, especially in immunocompromised patients and in those with risk factors la oncologic diseases. It can cause infections presented as pneumonia, meningitis, endocarditis or bacteremia, being the last two the most frequent ones. It can be highlighted its intrinsic resistance to vancomycin, which makes penicillin and other beta-lactam antibiotics the first treatment options. (AU)

Ferritin-mediated siRNA delivery and gene silencing in human tumor and primary cells.

We demonstrate a straightforward method to encapsulate siRNA into naturally available and unmodified human apoferritin. The encapsulation into apoferritin is independent of the sequence of the siRNA and provides superior protection for those sensitive molecules. High efficiency in transfection can be achieved in human tumorigenic cells, human primary mesenchymal stem cells (hMSC) and peripheral blood mononuclear cells (PBMCs). In contrast to Lipofectamine, highly effective gene silencing can be achieved with ferritin as the delivery agent in both tumor cells and PBMCs at low siRNA concentrations (10 nM). As an endogenous delivery agent, apoferritin does not induce immune activation of T- and B-cells in human PBMCs. Apoferritin shows intrinsic anti-inflammatory effects and apoferritin-mediated delivery shows a preference for immune-activated T- and B-cells, a natural selectivity which may turn useful for drug delivery in case of infections or inflammatory diseases.