RESUMO
AzuFluor® 435-DPA-Zn, an azulene fluorophore bearing two zinc(II)-dipicolylamine receptor motifs, exhibits fluorescence enhancement in the presence of adenosine diphosphate. Selectivity for ADP over ATP, AMP and PPi results from appropriate positioning of the receptor motifs, since an isomeric sensor cannot discriminate between ADP and ATP.
Assuntos
Difosfato de Adenosina/análise , Azulenos/química , Corantes Fluorescentes/química , Humanos , Estrutura Molecular , Espectrometria de FluorescênciaRESUMO
Azulene is a bicyclic aromatic chromophore that absorbs in the visible region. Its absorption maximum undergoes a hypsochromic shift if a conjugated electron-withdrawing group is introduced at the C1 position. This fact can be exploited in the design of a colorimetric chemodosimeter that functions by the transformation of a dithioacetal to the corresponding aldehyde upon exposure to Hg2+ ions. This chemodosimeter exhibits good chemoselectivity over other metal cations, and responds with an unambiguous colour change clearly visible to the naked eye. Its synthesis is concise and its ease of use makes it appropriate in resource-constrained environments, for example in determing mercury content of drinking water sources in the developing world.
RESUMO
Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.
Assuntos
Azulenos/química , Corantes Fluorescentes/química , Microscopia de Fluorescência por Excitação Multifotônica/métodos , Espécies Reativas de Nitrogênio/análise , Espécies Reativas de Oxigênio/análise , Azulenos/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Corantes Fluorescentes/toxicidade , Células HeLa , Humanos , Limite de DetecçãoRESUMO
Low cost and in situ fluoride detection by non-experts is important for the determination of drinking water safety in developing countries. Colour reagents can provide results quickly without expensive equipment, but colorimetric fluoride indicators are often nonspecific, complex to use or do not work in water. Here we show that azulene-boronate indicators respond selectively to fluoride at concentrations relevant to the WHO limit of 1.5 mg L-1.