Assuntos
Reabilitação Vocacional , Atitude do Pessoal de Saúde , Comunicação , Atenção à Saúde , Europa (Continente) , Financiamento Governamental , Órgãos Governamentais , Planejamento em Saúde , Humanos , Propriedade , Prática Profissional , Problemas Sociais , Estados Unidos , Instituições Filantrópicas de SaúdeRESUMO
Nutrient agar medium is made highly toxic to certain repair-deficient strains of Escherichia coli by exposure of the uninoculated plates to radiation from cool-white fluorescent lamps or black-light fluorescent lamps. This sensitivity is associated with the genetic deficiencies, fil, phr, and recA. Repair-sufficient and uvr strains are only slightly affected by the irradiated media. The poor growth and reduced plating efficiency frequently associated with BPhr and recA strains are very likely caused by inadvertent exposure of the medium to fluorescent light.
Assuntos
Meios de Cultura/efeitos da radiação , Escherichia coli/crescimento & desenvolvimento , Ágar , Aminoácidos , Sobrevivência Celular , Reparo do DNA , Escherichia coli/metabolismo , Glucose , Luz , Mutação , Efeitos da Radiação , Recombinação GenéticaRESUMO
A vapor arc light source has been adapted to the study of the lethal action on bacteria of near-ultraviolet (UV) and visible light. Its use makes possible much shorter exposure times than could be obtained from previously available sources. The output of radiant energy is sufficient to provide a fairly detailed action spectrum for lethality in the long-UV and visible region without the addition of exogenous sensitizers. Populations of cells of Escherichia coli WP2 were inactivated through five log(10) cycles with light at 460 nm. Significant inactivation also was obtained with light at 550 and 650 nm.