1.
Analyst
; 115(5): 617-21, 1990 May.
Artigo
em Inglês
| MEDLINE
| ID: mdl-2396748
RESUMO
A procedure for the isolation of glutamate dehydrogenase (GDH) from human liver, which involves the use of ion-exchange chromatography on diethylaminoethyl cellulose and affinity chromatography on guanosine triphosphate conjugated to Sepharose 4B, is described. The adsorptive voltammetric behaviour of human GDH, bovine GDH and rabbit anti-human GDH antibody was optimised with respect to accumulation potential, accumulation time and scan rate. The lower limits of detection were 0.2 and 1.2 mg l-1 for human and bovine GDH, respectively, and the lower limit of detection for rabbit anti-GDH antibody was 0.04 mg l-1. The interaction of human GDH with rabbit anti-human GDH antibody was also examined using this method.
Assuntos
Glutamato Desidrogenase/isolamento & purificação , Animais , Reações Antígeno-Anticorpo , Bovinos , Glutamato Desidrogenase/imunologia , Humanos , Fígado/enzimologia , Coelhos/imunologia
2.
Analyst
; 113(2): 317-9, 1988 Feb.
Artigo
em Inglês
| MEDLINE
| ID: mdl-3377174