Causal association between genetically predicted circulating immune cell counts and frailty: a two-sample Mendelian randomization study.

Background: Despite the recognized link between immune responses and frailty, the association between immune cell counts and frailty based on previous observational studies remains disputed, with uncertain causal nexus. This study aimed to elucidate causal association between genetically predicted circulating immune cell counts and frailty. Methods: We conducted the two-sample Mendelian randomization (MR) study with independent genetic variants associated with six immune cell subtype counts from genome-wide association studies in 563,946 European individuals. Frailty summary data, assessed via frailty index (FI), was obtained from study comprising 175,226 subjects. Univariate MR, reverse MR and multivariate MR were conducted to comprehensive investigate the association between immune cell counts and FI, with two-step MR analysis for mediation analysis. Results: Univariate MR evidence indicated that among six leukocyte subtype counts, only elevated eosinophil count was significantly correlated with higher FI (ß = 0.059, 95% confidence interval [CI], 0.042-0.078, P=5.63E-11), with no reverse causal relationship identified in reverse MR. In multivariate MR, the causal effect of eosinophil count retained statistical significance (ß = 0.063, 95% CI, 0.021-0.104, P = 0.003). Ultimately, the two-step MR analysis demonstrated two mediators in this causal pathway: asthma (ß= 0.019, 95% CI, 0.013-0.025, P = 35.84E-10, mediated proportion, 31.732%) and rheumatoid arthritis (ß= 0.004, 95% CI, 0.001-0.006, P=1.75E-03, mediated proportion, 6.411%). Conclusions: Within immune cell subtypes, MR evidence indicated only genetically predicted circulating eosinophil count had irreversible and independent causal effect on frailty, with asthma and rheumatoid arthritis possibly serving as partial mediators. The finding stressed the need for further exploring physiological functions of eosinophils in order to develop effective strategies against frailty.

Forgiveness weakens women's counter-empathy both in the stage of self-oriented and other-oriented emotional responses.

Counter-empathy may have more serious negative effects on women than on men. Sixty-four female Chinese university students participated in this study. Based on the counter-empathy model in a competitive setting, we examined how forgiveness affects counter-empathy. We manipulated the degree of participants' forgiveness toward their opponents by changing the type of information they received from their opponents. We also measured counter-empathy through self-reported pleasantness caused by opponents' facial expressions (smiles or frowns). We found that forgiveness can effectively alleviate women's counter-empathy, restraining their emotional experience from being inconsistent with the offender and enhancing their empathic concern toward the offender. We preliminarily explored how forgiveness works on counter-empathy and provided insight into the use of forgiveness to maintain global women's emotional health.

Quality evaluation index development for nutritional management in patients with nasopharyngeal carcinoma during peri-radiotherapy.

OBJECTIVES: It is necessary to construct an evaluation index for patients with nasopharyngeal carcinoma during peri-radiotherapy to provide a reference for the evaluation of the quality of nutritional management of patients with nasopharyngeal carcinoma during peri-radiotherapy. The aim of this study was to construct a set of scientific, comprehensive, and feasible indicators for evaluating the quality of nutrition management in patients with nasopharyngeal carcinoma during peri-radiotherapy to provide a unified reference basis for objective nutritional evaluation of these patients during the peri-radiotherapy period and to provide insights to the clinical treatment and care of these patients. METHODS: A multidisciplinary research team was set up from December 2021 to April 2022. We took the three-dimensional quality structure model as the theoretical framework; based on the literature review, the first draft of the nutrition management quality evaluation index for patients with nasopharyngeal carcinoma during peri-radiotherapy was formed by a semi-structured interview. The Delphi correspondence method was used to survey 18 experts from 12 cities in China. The multidimensional analytical hierarchy process was used to determine the evaluation index and weight of nutrition management quality of patients with nasopharyngeal carcinoma during peri-radiotherapy. RESULTS: The effective questionnaire recovery rates of the two rounds of letters were 90.005% and 100%, respectively, and the expert authority coefficients were 0.906 and 0.918, respectively. The Kendall harmony coefficients of the two rounds of letters were 0.271 to 0.313 and 0.309 to 0.349, respectively. The nutrition management quality evaluation index of patients with nasopharyngeal carcinoma during peri-radiotherapy was constructed and included 3 first-level indexes, 10 second-level indexes, and 71 third-level indexes. CONCLUSION: The evaluation index of the nutrition management quality of patients with nasopharyngeal carcinoma during peri-radiotherapy is scientific and reliable, and it may have a certain guiding significance for nurses to evaluate the quality of nutrition management of these patients during this period.

Greater resting frontal alpha asymmetry associated with higher emotional expressive flexibility.

Emotional expressive flexibility (EEF) is an important social ability that has prompted scholars to examine its benefits to human mental health. However, the neural underpinnings of individual differences in the EEF remain unclear. In neuroscience, frontal alpha asymmetry (FAA) is regarded as a sensitive indicator of certain emotional modalities and affective styles. To the best of our knowledge, no study has linked FAA with EEF to examine whether FAA could be a potential neural indicator of EEF. In the present study, 47 participants (Mage = 22.38 years, 55.3% women) underwent a resting electroencephalogram and completed the flexible regulation of emotional expression scale (FREE). The results revealed that after controlling for gender, resting FAA scores positively predicted EEF, with relative left frontal activity associated with higher EEF. Additionally, this prediction was reflected in both the enhancement and suppression dimensions of EEF. Furthermore, individuals with relative left frontal activity reported greater enhancement and EEF than individuals with relative right frontal activity. The present study indicated that FAA may be a neural marker of EEF. In the future, more empirical studies are needed to provide causal evidence that the improvement in FAA can enhance EEF.

Empathy or Counter-Empathy? The Victims' Empathic Response Toward Offenders Depends on Their Relationships and Transgression Severity.

Introduction: Empathy facilitates prosocial behaviors, whereas counter-empathy harms others. The question that remains unanswered is: when and for whom do people show different empathic responses? This study aimed to explore the effects of transgression severity and interpersonal relationships on victims' empathy or counter-empathy toward an offender. Methods: Before and after experiencing a slight or serious transgression, 42 college students were asked to imagine that they had different relationships (ie, intimate, strange, or bad) with a person and then report their empathy or counter-empathy toward that person from cognitive and affective aspects. Results: The results showed that, in the affective aspect, the participants' empathy for the intimate friend decreased after a slight transgression and even disappeared after a serious transgression. For strangers, empathy transformed into counter-empathy after the transgression, and its intensity increased with the transgression's severity. For a person in a bad relationship, the participants felt counter-empathy before the transgression, and its intensity increased with the transgression's severity. In the cognitive aspect, participants' counter-empathy toward the stranger and the person in a bad relationship increased with transgression severity. Discussion: These results suggest that interpersonal relationships and transgression severity can change the type and degree of a victim's empathy toward the offender. Our findings not only deepen our understanding of the cognitive aspect of counter-empathy but also provide insights for handling interpersonal conflict.

Functional studies of four MiFPF genes in mango revealed their function in promoting flowering in transgenic Arabidopsis.

Flowering promoting factor (FPF) genes play a substantial regulatory role in the process of flowering. In the present study, four MiFPF genes, namely, MiFPF1, MiFPF2, MiFPF3a, and MiFPF3b, were obtained from mango (Mangifera indica L.). Tissue expression analysis showed that MiFPFs were expressed in all mango tissues. Specifically, MiFPF1 and MiFPF2 were highly expressed in leaves, while MiFPF3a and MiFPF3b were highly expressed in flowers and buds. The four MiFPF proteins localize to the nucleus. Overexpression of MiFPFs in transgenic Arabidopsis resulted in early flowering and upregulated the expression of APETAL1 (AP1), FLOWERING LOCUS D (FD) and FLOWERING LOCUS T (FT). MiFPF genes increased the root growth of transgenic Arabidopsis plants under gibberellin treatment. BiFC assays showed that MiFPFs can interact with several DELLA proteins. Taken together, our results demonstrate that the MiFPF gene was involved not only in promoting flowering but also in increasing root growth under gibberellin (GA3) treatment.

Evaluation of effect of horticultural therapy on improving mental health in university students / 中国学校卫生

Objective@#To investigate the effectiveness of horticultural therapy on improving university students mental health problems and to provide evidence to support the application of horticultural therapy in the prevention and treatment of university students mental health.@*Methods@#From March to May 2022,a before-and after paired design was used with a blank control group, mental health screening abnormalities were recruited from a comprehensive university in Hebei Province, including 57 in the intervention group and 21 in the control group, and the Symptom Checklist-90(SCL-90) score and hair cortisol were included as outcome indicators, which was measured by enzyme linked immunoassay Elisa double antibody sandwich method.@*Results@#The total SCL-90 scores of the intervention group decreased ( t=4.28, P <0.01) and were significantly lower compared with the control group( t=-2.66, P <0.01), while no significant difference was observed in the control group( t=0.29, P >0.05), and the difference between the pre and post measures on each dimension of the SCL-90 was significantly lower in the intervention group (all P <0.01); the difference between the pre and post measures of hair cortisol contentration in the intervention group was statistically significant( Z= -2.75, P <0.05), and the hair cortiso contentration in pre measure group was lower than that of control group( Z=-3.48, P < 0.01 ). In contrast, the difference between pre and post measurements of hair cortisol in the control group was not significant( Z= -0.75 , P >0.05).@*Conclusion@#Horticultural therapy can improve the psychological symptoms of university students is suitable for psychological interventions in the university population and is beneficial to the health and well being of university students.

"I'm listening, did it make any difference to your negative emotions?" Evidence from hyperscanning.

Hyperscanning refers to simultaneously recording the brain activity of two or more people participating in the same cognitive activity to reveal the underlying processes. Active listening is a necessary and important part of interpersonal emotional regulation; however, few studies have addressed the corresponding brain activity. Therefore, this study aims to explore the regulatory effect of active listening and changes in the brain using functional near-infrared optical spectroscopy(fNIRS) in real situations requiring interpersonal emotional regulation. Behavioral results show that active listening has a significant effect on improving individuals' negative emotions. According to the neuroimaging results, the orbitofrontal cortex (OFC), right dorsolateral prefrontal cortex (right dlPFC), right temporoparietal junction (right TPJ), and left dorsolateral prefrontal cortex (left dlPFC) were significantly activated. In addition, band analysis showed interpersonal brain synchronization (IBS) increments at the orbitofrontal cortex (OFC), right dlPFC, right TPJ, and left dlPFC at different frequencies. Measurements of IBS and behavioral coherence showed that the increases of IBS at the OFC, right dlPFC, right TPJ, and left dlPFC were not significantly correlated with depression, anxiety, and the empathy level of the emotional regulator. The present study provides brain imaging evidence for the effectiveness of active listening in interpersonal emotional regulation.

Construction of MicroRNA-mRNA Regulatory Network in the Molecular Mechanisms of Bleomycin-Induced Pulmonary Fibrosis.

Bleomycin is a common antitumor agent used to treat many different types of malignancies; however, its main side effect is pulmonary fibrosis. The mechanism of bleomycin-induced pulmonary fibrosis (BIPF) has not been fully elucidated. Therefore, to further explore the molecular mechanisms of BIPF, we screened for microRNA (miRNA) and mRNA expression obtained from BIPF samples from the Gene Expression Omnibus database. Subsequently, we identified the differentially expressed miRNAs and genes that overlapped with the differentially expressed miRNAs target genes, predicted by using the miRWalk database selected as a candidate. The candidate genes were visualized based on Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes analyses. A protein-protein interaction network was constructed. Hub differentially expressed genes were selected and corresponding miRNAs to construct a miRNA-mRNA regulation network. Then, we chose three key miRNAs to study their regulatory relationship in bleomycin-induced pulmonary fibrosis. Finally, mouse lung epithelial cells TC-1 and MLE-12 were treated with bleomycin with qPCR to validate the results of three important hub genes and all key miRNAs. And dual-luciferase report experiment was carried out to verify the interaction of mmu-miR-1946a and serpina3n. The results revealed that the imbalance of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) plays a pivotal role in the occurrence and development of BIPF. In addition, Serpina3n and mmu-miR-1946a were proved interaction and may be involved in the regulation of the balance between MMP-9 and TIMP-1. The experimental results also verify the analysis. Our findings provide new insights into the key mediators and pathways related to the molecular mechanisms of BIPF.

Low-dose paclitaxel modulates the cross talk between the JNK and Smad signaling in primary biliary fibroblasts.

OBJECTIVE: The objective of this study was to explore the molecular mechanism underlying the occurrence of benign bile duct stricture and the target of low-dose paclitaxel in the prevention of benign bile duct stricture. METHODS: Under the stimulation of transforming growth factor beta 1, the expression of collagen type I and connective tissue growth factor were detected on isolated primary fibroblasts. The phosphorylation levels of JNK and Smad2L were detected using Western blot. The effect of low-dose paclitaxel on the transforming growth factor beta 1-induced inhibition of type I collagen and connective tissue growth factor expression and JNK and Smad2L phosphorylation was also observed. RESULTS: Transforming growth factor beta 1 induced the secretion of type I collagen and connective tissue growth factor as well as JNK phosphorylation in biliary fibroblasts. The JNK inhibitor or siRNA-Smad2 inhibited the transforming growth factor beta 1-induced secretion of type I collagen and connective tissue growth factor. Low-dose paclitaxel inhibited the expression of type I collagen induced by transforming growth factor beta 1 and may inhibit the secretion of collagen in biliary fibroblasts. CONCLUSION: The activation of JNK/Smad2L induced by transforming growth factor beta 1 is involved in the occurrence of benign bile duct stricture that is mediated by the overexpression of type I collagen and connective tissue growth factor, and low-dose paclitaxel may inhibit the phosphorylation of JNK/Smad2L.

Collective self-referential processing evoked by different national symbols: An event-related potential study.

The collective self is an important representation of self-concept, especially for people in collectivism culture. However, it is not clear whether there are differences in the self-reference effects caused by different collective self-relevant stimuli. The present study aimed to explore the temporal characteristics of collective self-referential processing evoked by polarized and unpolarized national symbols. Event-related potentials (ERPs) were recorded for pictures of national symbols and self-irrelevant pictures when 25 female participants performed a three-stimulus oddball task. The results indicate that compared to self-irrelevant pictures, both national symbols elicited collective self-reference effects on N2, P3, and LPP amplitudes. Polarized and unpolarized national symbols showed differences in N2 and P3 amplitudes. Moreover, national identity level was correlated with N2 and P3 amplitudes elicited by unpolarized symbols, and early LPP amplitudes elicited by both symbols. These results suggest greater recruitment of resources to process national symbols, and inconsistent time courses of processing different national symbols. Polarized symbols may consume more resources because of the internal complexity of their self-representations. The present study expands the research on collective self and its self-referential effect on women, and provides some enlightenment for understanding the internal factors that influence the strength of the self-reference effect.

Low-dose paclitaxel modulates the cross talk between the JNK and Smad signaling in primary biliary fibroblasts

SUMMARY OBJECTIVE: The objective of this study was to explore the molecular mechanism underlying the occurrence of benign bile duct stricture and the target of low-dose paclitaxel in the prevention of benign bile duct stricture. METHODS: Under the stimulation of transforming growth factor beta 1, the expression of collagen type I and connective tissue growth factor were detected on isolated primary fibroblasts. The phosphorylation levels of JNK and Smad2L were detected using Western blot. The effect of low-dose paclitaxel on the transforming growth factor beta 1-induced inhibition of type I collagen and connective tissue growth factor expression and JNK and Smad2L phosphorylation was also observed. RESULTS: Transforming growth factor beta 1 induced the secretion of type I collagen and connective tissue growth factor as well as JNK phosphorylation in biliary fibroblasts. The JNK inhibitor or siRNA-Smad2 inhibited the transforming growth factor beta 1-induced secretion of type I collagen and connective tissue growth factor. Low-dose paclitaxel inhibited the expression of type I collagen induced by transforming growth factor beta 1 and may inhibit the secretion of collagen in biliary fibroblasts. CONCLUSION: The activation of JNK/Smad2L induced by transforming growth factor beta 1 is involved in the occurrence of benign bile duct stricture that is mediated by the overexpression of type I collagen and connective tissue growth factor, and low-dose paclitaxel may inhibit the phosphorylation of JNK/Smad2L.

Epithelial-mesenchymal Transition of Peritoneal Mesothelial Cells Is Enhanced by M2c Macrophage Polarization.

BACKGROUND: Peritoneal fibrosis (PF) can reduce the efficiency of peritoneal dialysis and eventually lead to ultrafiltration failure. Epithelial-mesenchymal transition (EMT) of peritoneal mesothelial cells (PMCs) is the start of PF. Macrophages are involved in the process. This study was to investigate the effect of macrophage polarization on EMT of PMCs. METHODS: Monocyte-macrophage cells (THP-1) were treated to induce macrophage subsets (M1, M2a, M2c). The inducing was assessed by detecting protein and mRNA expression of cytokines using ELISA and RT-PCR. Subsequently, PMCs were co-cultured with M1, M2a and M2c, respectively, in Transwell chambers for 48 h and then expressions of E-cadherin and α-SMA were determined in PMCs. The PMCs that were not co-cultured with macrophages served as control PMCs. One-way ANOVA and SNK-q test were used to conduct statistics and P < .05 as significant. RESULTS: Detection of the cytokines, including IL-6, IL-10, IL-12, TGF-ß1, CCL17 and CXCL13, verified that the inducting of macrophage subtypes was successful. Compared to control, E-cadherin protein expression was significantly decreased and α-SMA protein expression increased in M1-treated PMCs (P < .05); M2a-treated PMCs had an increased gene expression of α-SMA (P < .05); E-cadherin protein and gene expression were decreased and α-SMA protein and gene expression increased significantly in M2c-treated PMCs (P < .05 or P < .01). CONCLUSIONS: EMT of PMCs is enhanced by M2c macrophage polarization; meanwhile, M1 and M2a polarization may have the effect to some extent, but not as definite as M2c.

Forgiveness weakens counterempathy at the early and late stage of empathic responses to opponents' expressions.

Our emotional response to people is discordant with their emotional experience in competitive situations; this phenomenon is termed "counterempathy." Using event-related potentials, this study investigated the neural underpinnings of the effect of forgiveness on counterempathy. Twenty-seven female university students participated in a two phase-interpersonal competitive game with two other players whose smiles and frowns indicated the participant's losing and winning, respectively. In the "passive" phase, participants were passively punished with a high- or low-intensity noise chosen by the opponent each time they lost a trial (i.e., the opponent smiles). During the break, participants received a negative or friendly message from each opponent. Participants were more likely to forgive the opponent who had sent a friendly message. In the "active" phase, participants could punish both opponents when they won a trial (i.e., the opponent frowns). Behavioral data showed that participants' empathic responses were inconsistent with the opponents' expressions, and that forgiveness could weaken this effect. The electrophysiological data revealed that both very early emotional sharing (reflected in the N170) and late elaborative cognitive evaluation stage (reflected in the P300) of counterempathy were affected by forgiveness, whereas the early automatic cognitive evaluation stage (reflected in the feedback-related negativity [FRN]) was not. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

"Watching Eyes" Triggers Third-Party Punishment: The Role of Emotion Within the Eyes.

Third-party punishment refers to a behavioral phenomenon whereby people punish wrongdoers even if their sanction incurs personal costs but yields no direct benefits. Given the eye cues demonstrated ability to convey signals of being observed, its effect on third-party punishment, driven by virtue of its effects on others' perceptions, was investigated. In addition, emotional message featured in the eye region is crucial in social interaction, whether the emotion within the eyes serves this effect with varying degrees of influence has rarely considered. The present study aimed at exploring (a) the watching eyes effect on the third-party punishment and (b) whether this effect varies from negative eyes to positive eyes. By two experiments using a modified Third-Party Dictator Game, we displayed either eye images or control images above the question on whether to punish the dictators or not. There was no emotional diversity of eye cues in Experiment 1, and most participants tended to punish for unfair offer. However, the appearance of eye images increased the punishment relative to control images. In Experiment 2, the eye cues were subdivided into positive and negative. The effect of watching eyes on the third-party punishment was significantly stronger when the eyes were negative than positive. Results revealed that eye cues play a role in promoting the third-party punishment and offer a potential insight into the mixed findings, such that the emotion within the eyes, especially the negative expression in the eyes, may influence the watching eyes effect.

The influence of relationship closeness and desire for forgiveness on interpersonal forgiveness.

This study aimed to investigate the moderating effect of the desire for forgiveness on the association between relationship closeness and interpersonal forgiveness using event-related potentials (ERPs). We recruited forty student participants (20 males and 20 females) to complete the modified Taylor Aggression Paradigm (TAP), which was used to induce the offensive experience and record the victim's level of reactive aggression toward the offenders. Results indicated that for the internal intention of forgiveness behavior, when the desire for forgiveness was not expressed, participants were more forgiving of close offenders than strangers; when the desire for forgiveness was expressed, the internal intention of forgiveness behavior for strangers was similar to that for close offenders. Regarding the external performance of forgiveness behavior, whether the offender expressed the desire for forgiveness or not, participants exhibited more external performance of forgiveness behaviors toward the close offenders than the strangers. Furthermore, the decision-related negativity (DRN)-a negative component associated with aggressiveness-was larger when participants were considering the punishment of strangers when no desire for forgiveness was expressed. There was no significant difference when the desire for forgiveness was expressed. Moreover, the feedback-related negativity (FRN)-a negative component reflecting the evaluation of the valence of an outcome-was larger when participants lost a trial against strangers when no desire for forgiveness was expressed. No such difference was observed in both opponents when the desire for forgiveness was expressed. These findings confirmed that the desire for forgiveness moderated the association between relationship closeness and interpersonal forgiveness.

The Influence of Emotional Awareness on Time Perception: Evidence From Event-Related Potentials.

Prior studies found that participants overestimated both negative and positive emotional stimuli, compared with neutral emotion. This phenomenon can be explained by the "arousal mechanism." Participants demonstrated individual differences in emotion perception. In other words, high emotional awareness resulted in high emotional arousal, and vice versa. This study extended existing findings by exploring the influence of emotional awareness on time perception in a temporal generalization task, while recording electroencephalographic (EEG) signals. The findings revealed that in the positive emotion condition, the high emotional awareness group made more overestimations, compared with the low emotional awareness group. However, no difference was observed in the neutral or negative emotion conditions. Moreover, the event-related potential (ERP) results showed that in the positive emotion condition, the high awareness group elicited larger vertex positive potential (VPP) amplitudes, compared with that of the low awareness group. However, no such differences were observed in the neutral and negative emotion conditions. Moreover, the contingent negative variation (CNV) (200-300, 300-490 ms) component showed that in the positive emotion, the amplitudes of the high awareness group were larger than that of the low awareness group; however, they did not show differences in the neutral condition. The findings of this study suggest that high emotional awareness produces higher physiological arousal; moreover, when participants were required to estimate the time duration of emotional pictures, they tended to make higher time overestimation. Thus, our results support the relationship between emotional awareness and time perception.

[Metformin inhibits collagen production in rat biliary fibroblasts: the molecular signaling mechanism].

OBJECTIVE: To clarify the molecular signaling mechanism underlying the inhibitory effect of metformin on transforming growth factor-ß1 (TGF-ß1)-stimulated collagen I production in rat biliary fibroblasts. METHODS: Primary biliary fibroblasts were isolated under aseptic condition from 50 Sprague-Dawley rats (half male and half female), and microscopic observation identified no obvious difference in the morphology or viability of the cells from rats with different sexes or body weight. The cells were treated with TGF-ß1 (10 ng/mL), Smad3 siRNA+TGF-ß1, CTGF siRNA+TGF-ß1, metformin (10 mmol/L)+ TGF-ß1, or Compound C (10 µmol/L)+metformin+TGF-ß1. The expressions of CTGF and collagen I in the treated cells were determined using ELISA kit or Western blotting; the phorsphorylated and total Smad3 and AMPK expressions were detected using immunoblotting. RESULTS: TGF-ß1 time- and dose-dependently induced collagen I production in rat biliary fibroblasts. The activated AMPK by metformin dose-dependently inhibited TGF-ß1-induced collagen I production. Pre-incubation of cells with the AMPK inhibitor Compound C restored the inhibitory effect of AMPK on TGF-ß1-induced collagen I secretion (P < 0.01). Activation of AMPK by metformin significantly reduced TGF-ß1-induced collagen I production by suppressing Smad3-driven CTGF expression (P < 0.01), and the application of Compound C reversed such changes in the fibroblasts (P < 0.01). CONCLUSIONS: Metformin inhibits TGF-ß1-stimulated collagen I production by activating AMPK and inhibiting Smad3- driven CTGF expression in rat biliary fibroblasts.

Ag-Embedded Silica Core-Shell Nanospheres for Operando Surface Enhanced Raman Spectroscopy of High-Temperature Processes.

The construction of thermally robust, highly active, and universal substrate architectures is a major challenge for high-temperature operando studies using surface enhanced Raman spectroscopy (SERS). Herein, a novel hybrid nanostructure of embedded Ag nanoparticles confined by a core-shell silica nanosphere through facile chemical synthesis is reported. Benefiting from the coupling effect of embedded Ag nanojunctions and the nanoconfinement of the silica core-shell, the hybrid nanospheres exhibit strong SERS-enhancement effects and thermal stability without restrictions on the substrate generality. Three-dimensional finite-difference time-domain (3D-FDTD) calculation indicates that the self-assembled nanojunctions of the embedded Ag nanoparticles facilitate a strong amplification of the electromagnetic field on individual nanospheres. The measurements on the trace analysis of the carbon species and dynamic tracking of the ceria lattice illustrate the feasibility of the hybrid nanospheres for the operando analysis of high-temperature processes, especially for trace detection of critical surface species or dynamic tracking of local structure evolution.

Prognostic Value of CT Imaging-Based Tumor Volume in Patients With Non-Surgical Esophageal Squamous Cell Carcinoma.

BACKGROUND: The American Joint Committee on Cancer-Tumor (AJCC-T) staging system for esophageal carcinoma patients, which is based on the depth of tumor invasion, is not applicable in some cases. This study aims to assess the prognostic value of CT imaging-based tumor volume and its usefulness for T staging in patients with non-surgical esophageal squamous cell carcinoma (ESCC). METHODS: We retrospectively reviewed the records of 158 ESCC patients undergoing definitive (chemo) radiotherapy from two hospitals. Tumor volume based on the CT imaging was calculated using the formula: V = πabc / 6. Three cutoff points for tumor volume were obtained with the X-tile software. Overall survival (OS) was analyzed using the Kaplan-Meier method. The -2 log-likelihood ratio and Akaike Information Criterion (AIC) value were evaluated to compare the AJCC-T staging system with the proposed T staging method. RESULTS: The median tumor volume was 19.8 cm³ (range from 1.0 to 319.5 cm³). The three optimal cutoff points of tumor volume were 12.7, 22.8, and 51.9 cm³, and the patients were divided into four groups named as proposed T1-T4 stages. The 3-year OS rates in patients with proposed T1 to T4 stages were 67.9%, 30.6%, 21.3%, and 5.3%, respectively. The -2 log-likelihood ratios of the AJCC-T stage and proposed T stage were 1,068.060 and 1,047.418, respectively. The difference in the AIC value between the two T staging systems was 18.642. CONCLUSION: CT imaging-based tumor volume was superior to the depth of tumor invasion for T staging in predicting the prognosis of non-surgical ESCC patient.