Nanoliter capillary electrochromatography columns based on collocated monolithic support structures molded in poly(dimethyl siloxane).

Following current trends in miniaturization of analytical chemistry, an inexpensive disposable analytical tool in the form of a liquid chromatography column fabricated on a poly(dimethyl siloxane) (PDMS) chip was created. Ease of fabricating the chromatography column was demonstrated by molding collocated monolithic support structures (COMOSS) directly in the column. Positive photo-resist, SPR 220, was used to create column structures on a negative relief master providing final channel dimensions of 2.7-5.2 microm wide by 10.0 microm deep, while monolithic dimensions were 9.8 x 9.8 x 10.0 microm - 12.3 x 12.3 x 10.0 microm. The ability to separate biological samples such as peptides from a tryptic digest of fluorescein isothiocyanate labeled bovine serum albumin (FITC-BSA) was shown. Separations in capillary electrochromatographic (CEC) mode were performed yielding column efficiencies of 4.0 x 10(5) plates/m.

[Use of a densitometric method for analysis of active substances in the drugs. XVI. Multicomponent drugs containing ascorbic acid]. / Zastosowanie pomiarów densytometrycznych do oznaczania skladników czynnych w lekach.

A densitometric method for the determination of active substances in Ascofer, Fenquil, Rutinoscorbin and Scorbolamid after separation of their components by means of TLC-has been elaborated. Usefulness of the method was checked by comparison of the obtained results with those afforded by means of reference methods.