Surfactant system and water balance of the lungs in modeling of neurodegeneration and focus of pathological activity in the substantia nigra.

The comparative analysis of surfactant metabolism and water balance of the lungs in modeling the formation and neurodegeneration focus of pathological activity in the substantia nigra of the brain. Methods: Experiments were performed on male rats - nonlinear, including the control, sham operated with unilateral stereotaxic administration of the neurotoxin 6-hydroxydopamine (Sigma) in the substantia nigra compact part and the implantation of cobalt metal nanopowder (Berlin) in the reticular part of the structure. Complex investigations included determination of surfactant phospholipid fractions by thin layer chromatography, total phospholipids and cholesterol in the bronchoalveolar lavage and surface-active properties by the Wilhelmy method, phospholipase activity, lipid peroxidation intensity on the content of TBA-active products in the lung tissue, evaluation of water balance using the gravimetric method. Results: It has been established that the introduction of intranigral neurotoxin and implantation of cobalt in the structure of the brain called the deterioration of the surface activity of the alveolar lining set against the backdrop of an imbalance of fractional composition of surfactant lipids with multidirectional changes in the amount of phospholipids and the degree of hydration of the lung. Induction focus of pathological activity in the substantia nigra was accompanied by an increase in alveolar phospholipids by lysophosphatidylcholine, sphingomyelin, phosphatidylethanolamine, intensification of lipid peroxidation of the lung tissue and organ hyperhydration. Modeling neurodegeneration - reduction of total phospholipids, phosphatidylcholine and lysophosphatidylcholine accumulation in terms of activation of phospholipase hydrolysis, increasing blood supply to the lungs. Conclusion: Metabolism of surfactant lipids and water balance in the lung, along with changes of rhythmogenesis and mode of ventilation, may lead to the development of disregulation of pneumopathy with dysfunction of the substantia nigra of the brain.

[NON-RESPIRATORY FUNCTION OF THE LUNGS IN THE NIGROSTRIATAL DOPAMINERGIC SYSTEM DYSFUNCTION].

The study presents a comprehensive of the metabolism and the fractional composition of li- pids surfactant, water balance, hemostatic activity of the lungs in neyrodegeneration substantia nigra of the brain induced by stereotaxic microinjection of 6--hydroxydopamine and systemic administration of haloperidol. It is shown that a breach of dopaminergic neurotransmission leads to deterioration of surface-active properties of the alveolar lining of the complex against a decrea- se of phospholipids, cholesterol, phosphatidylcholine and lysophospholipids enhance the pulmo- nary surfactant in the activation of phospholipase hydrolysis and lipid peroxidation. Intranigral introduction neurotoxin accompanied by increased blood supply to the lungs and the blood coagu- lation potential of the pulmonary circulation, the blockade D2-receptors--hyporhydration lung tissue. The results obtained indicate the formation of dysregulation pneumopathy dysfunction nigrostriatal dopaminergic system.

[Metabolic lung function upon activation of the substantia nigra and in conditions of gabaergic mediation of the amygdala and brainstem structures].

The study presents a comprehensive assessment of the integration of metabolic functions of the lungs when the focus of pathologic activity forms in the substantia nigra and when GABA is introduced into the central nucleus of the amygdala and into the giant cell reticular nucleus of the medulla oblongata. The experimental studies were conducted on laboratory rats. It was established that GABAergic mediation of efferent nigral influences contributes to the optimization of the surface-active properties of surfactant lipids and to the restoration of the blood supply and fluid balance in the lungs which were modified modelling the dysfunction of the substantia nigra and preserving the endothelial dysfunction in the pulmonary circulation.

[The effect of calcitrin and corticotropin on pulmonary surfactant and engorgement in rats during activation of the lateral hypothalamic area]. / Vliianie kal'tsitrina i kortikotropina na surfaktant i krovenapolnenie legkikh krys pri aktivatsii lateral'nogo gipotalamicheskogo polia.

The level of phospholipids, cholesterol, lipase activity in bronchoalveolar washes and lungs' blood capacity were studied in rats. An action exerted upon the hypothalamus led to a decrease in the surfactant lipids and the blood capacity of lungs. Possible peptidergic mechanisms of this are discussed.

[The effect of the lateral hypothalamic area on the pulmonary surfactant system]. / Vliianie lateral'nogo gipotalamicheskogo polia na surfaktantnuiu sistemu legkikh.

The level of lipids, lipase activity and lungs' blood capacity were studied on 62 rats. Dispersal cobalt was infused into the hypothalamic lateral area of rats with adrenalectomy or injected with droperidol, obsidan, reserpine. Their effect upon the hypothalamus involved a decrease in surfactant phospholipids and lung's blood capacity. Possible adrenergic mechanisms of affecting the lung surfactant system are discussed.