Research progress of porcine epidemic diarrhea virus S protein.

Porcine epidemic diarrhea virus (PEDV) is a single-stranded RNA virus with a capsid membrane that causes acute infectious gastrointestinal disease characterized by vomiting, diarrhea, and dehydration in swine. Piglets are more susceptible to PEDV than adults, with an infection rate reaching 90% and a fatality rate as high as 100%. Moreover, PEDV has a rapid transmission rate and broad transmission range. Consequently, PEDV has caused considerable economic losses and negatively impacted the sustainability of the pig industry. The surface spike (S) glycoprotein is the largest structural protein in PEDV virions and is closely associated with host cell fusion and virus invasion. As such, the S protein is an important target for vaccine development. In this article, we review the genetic variation, immunity, apoptosis-induction function, virulence, vaccine potential, and other aspects of the PEDV S protein. This review provides a theoretical foundation for preventing and controlling PEDV infection and serves as a valuable resource for further research and development of PEDV vaccines.

High-throughput measurement of coefficient of thermal expansion using a high-resolution digital single-lens reflex camera and digital image correlation.

High-throughput measurement of thermal deformation and determination of coefficient of thermal expansion (CTE) using a high-resolution digital single lens reflex (DSLR) camera and digital image correlation (DIC) is described. To mitigate the mosaic effect caused by the Bayer filter of DSLR cameras, a color image pre-processing method, which adjusts the brightness and equalizes the color channels of the raw image, is carried out. In addition, a Gaussian pre-filtering step is adopted for denoising the images captured with DSLR cameras to enhance the subpixel registration accuracy. Then, by processing the recorded images using the state-of-the-art DIC algorithm, full-field displacements and strains can be determined. Compared with conventional industrial cameras, a DSLR camera offers not only portability, compactness, and economy but also much higher resolution of recorded images, allowing CTE characterization with higher throughput. Real experiments, including a verification experiment of the color image pre-processing technique, a benchmark CTE determination of Al alloy, and a high-throughput CTE determination of 15 samples of three different metals, validated the feasibility and accuracy of the proposed technique. The proposed method is cost-effective and time-saving, showing great potential in the high-throughput CTE measurement and other high-throughput strain measurement scenarios.