ALDH5A1 acts as a tumour promoter and has a prognostic impact in papillary thyroid carcinoma.

Thyroid cancer is the most common endocrine carcinoma, with papillary thyroid carcinoma (PTC) accounting for 80%-90% of thyroid cancers. Accumulating studies reported that mitochondria plays an important role in the regulation of cell proliferation. ALDH5A1, may function as an oncogene or tumour suppressor in various human cancers, and the role of ALDH5A1 in PTC is still unclear. The aim of this study was to investigate the clinical significance of ALDH5A1 expression and its functions in PTC. In this present study, we studied ALDH5A1 expression on primary papillary thyroid carcinoma (PTC) in The Cancer Genome Atlas (TCGA) database. Results showed that the levels of ALDH5A1 were found positively correlated with tumour stage, metastasis, lymph node stage, and higher levels of ALDH5A1 demonstrated poor disease-free survival (DFS). Immunohistochemistry (IHC) revealed that significantly higher expression of ALDH5A1 was found in PTC tissues. On the other hand, knockdown of ALDH5A1 significantly inhibited PTC cell proliferation, migration and invasion detection found the migration and invasion of cells also were hindered when ALDH5A1 level was reduced. The knockdown of ALDH5A1 inhibited the expression of Vimentin and promoted the expression of E-cadherin. In brief, knockdown of ALDH5A1may act as a novel molecular target for the prevention and treatment of PTC. SIGNIFICANCE OF THE STUDY: The present study focused on the role and the potential mechanism of ALDH5A1 in papillary thyroid carcinoma. We demonstrated that reduced expression of ALDH5A1 might inhibit the progression of TC by inhibiting cell proliferation, migration and invasion and reversing epithelial-mesenchymal transition (EMT). The findings ensured the interaction relation between ALDH5A1 and EMT in PTC, providing a novel biological marker for PTC and enriching the potential strategies for TC treatment.

Characteristics and removal of microplastics in rural domestic wastewater treatment facilities of China.

The environment of the countryside is different from that of the city. Studying the abundance, characteristics, and removal of microplastics (MPs) in rural domestic wastewater treatment facilities (RD-WWTFs) is of great significance for understanding the impacts of human activities on the environment of the countryside. Therefore, we studied five such facilities in the Hangzhou region of China. The abundance of MPs in the influent was 430-2154 items/m3. Micro-Raman spectroscopic analysis indicates that the main type of polymer in the influent is polypropylene (PP, 54.6%), followed by polystyrene (PS, 29.7%) and polyethylene terephthalate (PET, 9.7%). The color of MPs is mainly white and clear (62.9%), red (13.3%) and gray (12.0%). Our results show that fragments (71.3%) are the dominant shape of MPs, followed by fibers (21.5%). The characteristics of MPs, such as sizes, shapes, and types, along with the treatment process, affect the removal of MPs in RD-WWTFs. Large MPs are easily removed by anaerobic processes, while small MPs are better removed by anaerobic/anoxic/oxic processes. Fibrous MPs are more difficult to be removed than the fragmented ones. Constructed wetlands play an important role in the removal of MPs.

[Nucleotide sequence analysis for a new HLA-B allele HLA-B*13:01:06*].

OBJECTIVE: To confirm a new allele HLA-B*13:01:06 and analyze its nucleotide sequence. METHODS: Genomic DNA was extracted using a Qiagen DNA extraction kit. Nucleotide sequences of HLA-A, HLA-B, HLA-C and HLA-DRB1 were analyzed by polymerase chain reaction-sequence based typing (PCR-SBT). HLA high-resolution results were assigned, and the nucleotide sequences of HLA-B locus was compared with that of HLA-B*13:01:01. RESULTS: The nucleotide sequence of the new allele shows a strong similarity to that of HLA-B*13:01:01. One nucleotide in exon 2 has changed from G to A at position 219 (codon 49 GCG>GCA), which however did not result in amino acid change. CONCLUSION: The novel allele verified by sequencing has been submitted to GenBank and officially named as HLA-B*13:01:06 by the World Health Organization HLA Nomenclature Committee.

[Full sequence analysis for a null allele of MICA gene (MICA*063N)].

OBJECTIVE: To analyze the full nucleotide sequence of a null allele of major histocompatibility complex class I chain-related gene (MICA). METHODS: A sequence-based typing method was used to determine the nucleotide sequence of the MICA gene. Potential alleles were identified with a computer program. RESULTS: The identified allele has possessed a sequence similar to that of MICA*027 except for a C→T substitution at position 184 in codon 62 (CAG→TAG) of exon 2. As a stop codon, this may result in a truncated protein. CONCLUSION: A null allele of MICA gene has been identified. The sequence has been submitted to the Genbank nucleotide sequence database (submission No. HWS10011131), which was officially named as MICA*063N by the WHO Nomenclature Committee in October 2010.