1.
J Am Chem Soc
; 135(17): 6399-402, 2013 May 01.
Artigo
em Inglês
| MEDLINE
| ID: mdl-23560498
RESUMO
Here we describe a novel surface that enables direct visualization of the hybridization of single DNA molecules with an unprecedented resolution using atomic force microscopy. The surface consists of single-stranded DNA probes that are covalently anchored to a self-assembled monolayer. The surface satisfies the contradictory requirements for high-resolution imaging and hybridization by switching the DNA-surface interaction between a strong state and a weak state. Our approach opens up unique opportunities in elucidating hybridization at the molecular scale.