Dermatophytosis caused by Trichophyton spp. in a Tenerife Lizard (Gallotia galloti): an immunohistochemical study.

Reports of dermatophytosis in reptiles are rare. This report describes the microscopical and immunohistochemical findings in a case of dermatophytosis caused by Trichophyton spp. in a 2-year-old Tenerife lizard (Gallotia galloti) with ulcerative and pustular skin lesions. Microscopically, the lesions were characterized by superficial epidermal pustules containing heterophils with numerous fungal hyphae that stained by periodic acid-Schiff and Grocott's stain. Fungal culture was not performed, but a panel of polyclonal antibodies specific for different fungal genera was applied to tissue sections. These immunohistochemical studies demonstrated reactivity of the hyphae only with antiserum specific for Trichophyton spp.

Climatic effects on Salmonella survival in plant and soil irrigated with artificially inoculated wastewater: preliminary results.

The use of wastewater to replace other water resources for irrigation is highly dependent on whether the health risk and environmental impacts entailed are acceptable or not. Total count and species of microorganisms found in wastewater vary widely because of climatic conditions, season, population sanitary habits and disease incidence. Salmonella, one of the genera associated with waterborne diseases, lives in the intestine. Thus, it is widely accepted that they have a limited survival period under environmental conditions. Wastewater management practices and the ability of Salmonella to survival under field conditions would determine the health risk associated with its presence in wastewater. Although chlorination is widely used, there are situations in which Salmonella is able to survive the sudden stress imposed by this technique. The aim of this experiment was to contribute to the study of the climatic and soil effects on pathogen survival under agricultural field conditions in order to assess which were the best wastewater management practices from both health and economic points of view. Five pots filled with soil seeded with Medicago sativa and an automatic weather station were used. A secondary effluent was artificially inoculated with Salmonella. In addition, open plates (filled with sterilised soil) and ultraviolet radiation isolated plates (filled with non-sterilised soil) were used. As soil heat emission contributes to the environmental conditions around the bacteria, standardised meteorological temperature data had to be carefully used in the bacterial survival studies under agricultural conditions. Radiation was the main cause of Salmonella mortality as its effect was more important than natural soil bacteria competence. Higher reduction of Salmonella counts could have been associated with longer spring days. Soil was able to effectively remove Salmonella. Subsurface drip irrigation methods could provide an effective tool to prevent health risk associated with wastewater irrigation.

[Carriers of beta hemolytic streptococci from groups A, B, and C among schoolchildren in Las Palmas]. / Portadores de estreptococos betahemolíticos de los grupos A, B y C en escolares de Las Palmas.

BACKGROUND: Betahemolytic streptococci, particularly group A, are the most frequently isolated pathogen in the cases of acute pharyngotonsilitis in school-aged children. A study was carry out in school children of Las Palmas de Gran Canaria with the aim of knowing of the state of pharyngeral carriers of betahemolytic streptococci in Lancefield groups A, B and C. METHODS: A total of 1,002 healthy school children (520 boys and 482 girls) with ages ranging from 4 to 15 of aged were included in the study in which a pharyngeal exudate was obtained to detect the presence of betahemolytic streptococci. RESULTS: 69.5% of the healthy school children were not pharyngeal carriers of betahemolytic streptococci. The prevalence of streptococci group A in all the age groups studied was of 6%. The prevalence of beta streptococci groups B and C were of 11 and 13.5%, respectively. CONCLUSIONS: The results of this study are in agreement with the literature reviewed in which the rate of pharyngeal carriers of beta-hemolytic streptococci in school children of Las Palmas de Gran Canaria are more similar that studies reported from tropical and subtropical zones showed a high prevalence of groups B and C and a lower prevalence of a group A than others communities of our country and zones with temperature climate where showed a high prevalence of group A and lower prevalence of groups B and C.

Caseous lymphadenitis caused by Corynebacterium ulcerans in the dromedary camel.

Caseous lymphadenitis that affected the dorsal and ventral superficial lymph nodes in the left cervicothoracic region of a young dromedary camel is described. The agent isolated was Corynebacterium ulcerans. To our knowledge, this is the first description of purulent lymphadenitis caused by C. ulcerans in a species belonging to the Camelidae.

[Sensitivity of groups A, B and C beta hemolytic streptococci to antibiotics]. / Sensibilidad a antibióticos en estreptococos beta hemolíticos de los grupos A, B y C.

We studied the susceptibility of 330 strains of beta hemolytic streptococci (60 group A, 125 group B, 145 group C) isolated from healthy school children in Las Palmas de Gran Canaria to 17 antibiotics. Only 2% of group A streptococci showed resistance to erythromycin, and 18% to 31% of the beta hemolytic streptococci strains were resistant to trimethoprim-sulfamethoxazole. All of these strains showed susceptibility to betalactam antibiotics, chloramphenicol, clindamycin and vancomycin. Most of them were resistant to tetracycline and aminoglycoside antibiotics.

Type I beta-lactamases of Enterobacter cloacae and resistance to beta-lactam antibiotics.

The interaction of type-I beta-lactamases from Enterobacter cloacae with diverse beta-lactam compounds was examined. The ability of penicillin and cefoxitin to induce beta-lactamase production in this strain was assessed. The effect of beta-lactamase inhibitors was measured on beta-lactamase extracts and on intact cells. E. cloacae 78 strain is a stably derepressed mutant showing limited susceptibility to a number of antibiotics except imipenem. Imipenem would therefore be the appropriate choice for therapy of infections caused by stably derepressed mutants of Enterobacter sp. producing type-I beta-lactamases.