Effects of Lecithin Supplementation in Feed of Different fat Levels on Serum Indexes and Liver Health of Laying Hens.

The aim of this experiment was to investigate the effect of soy lecithin on serum-related indicators and liver health in laying hens under the influence of high-fat diets. 180 peak laying hens at 40 weeks of age were randomly assigned to one of the four diets using a 2 × 2 factorial and fed for 5 weeks. The results showed that compared to the low-fat group, the high-fat group had lower egg production (p < 0.05) and higher average daily feed intake and feed-to-egg ratio (p < 0.05). At the 21st day, the serum levels of triglyceride (TC) and superoxide dismutase (SOD) were higher (p < 0.05), high-density lipoproteins cholesterol (HDL-C) levels were lower (p < 0.01), catalase (CAT) activity was lower (p < 0.05), TC and malondialdehyde (MDA) levels in liver were higher (p < 0.01) and SOD activity in liver was lower (p < 0.05) in layers supplemented with soy lecithin. CAT activity in serum was increased (p < 0.01) and total antioxidant capacity (T-AOC) activity in the liver was decreased (p < 0.05) after increasing the dietary fat concentration. The addition of soy lecithin and the increase in dietary fat concentration had a highly significant interaction on serum CAT activity and liver TC content in layers (p < 0.01). At the 35th day, the serum alanine aminotransferase (ALT) activity was higher (p < 0.01), serum glutathione peroxidase (GSH-Px) and CAT activity were higher (p < 0.05), and serum triglyceride (TG) content and total T-AOC capacity activity were lower (p < 0.05) in layers supplemented with soy lecithin. Increasing dietary fat concentration decreased alanine aminotransferase (ALT), aspartate aminotransferase (AST) and GSH-Px activity in serum (p < 0.05). However, it increased TG and MDA content in liver (p < 0.05), and highly decreased SOD content in liver (p < 0.01) in layers. The addition of soy lecithin and increasing dietary fat concentration had a highly significant reciprocal effect on serum ALT viability and CAT viability (p < 0.01) and liver TG and MDA content and SOD viability (p < 0.05) in layers. In conclusion, feeding high-fat diets will adversely affect the laying performance of laying hens, while long-term addition of lecithin can improve the blood lipids and liver lipids of laying hens, enhance the antioxidant capacity of the liver, and maintain liver health.

Effect of Dietary Ramie Powder at Various Levels on the Growth Performance, Meat Quality, Serum Biochemical Indices and Antioxidative Capacity of Yanling White Geese.

To investigate the effects of different levels of ramie powder (Boehmeria nivea (L.) Gaudich.) (i.e., 0%, 6%, 12% and 24%) on the production performance, serum biochemical indices, antioxidative capacity and intestinal development of Yanling white geese, a total of 256 geese at 56 days of age were randomly divided into four groups and fed a control diet and the control diet supplemented with 6%, 12% and 24% ramie powder, respectively, for 42 days. The results show that dietary supplementation with 12% ramie powder significantly increased the average final weight (p < 0.05) and tended to improve the average daily gain (ADG) and feed/gain ratio (F/G) of the test geese (0.05 < p < 0.10). Moreover, the dietary inclusion of 12 and 24% ramie powder improved meat qualities by reducing the L* value (p < 0.05) and cooking loss (0.05 < p < 0.10) of thigh muscle. Compared with the control group, the ramie powder supplementation at different levels increased the serum activities of glutathione peroxidase and glutathione, promoting the antioxidative capacity of the body (0.05 < p < 0.10). This study demonstrates that moderate ramie powder is beneficial to the production performance of Yanling white geese and has the potential to be used as a poultry feed ingredient. In conclusion, 12% was the proper supplementation rate of ramie powder in Yanling white geese feed.

Effects of Different Levels of Garlic Straw Powder on Growth Performance, Meat Quality, Antioxidant and Intestinal Mucosal Morphology of Yellow-Feathered Broilers.

The full utilization of garlic straw can partially alleviate shortage of feedstuff and waste of resources. The purpose of this study was to investigate the effects of garlic straw as an unconventional feed on yellow-feathered broilers. 360 28-day-old yellow-feathered broilers were randomly divided into 4 groups with 6 replicates (cage) per group, 15 per cage. The 4 groups were as follows: control group (basal diet) and experimental group I (basal diet supplemented with 3% garlic straw powder), II (basal diet supplemented with 6% garlic straw powder) and III (basal diet supplemented with 9% garlic straw powder). There was no significant difference in the initial body weight of the broilers among groups (p > 0.05). The test period was 28 days in total. The experiment results showed that there were no significant difference in the average final weight, ADG, ADFI and F/G among groups (p > 0.05). On the one hand, for the breast muscle, the drip loss of experimental group I, II and III were reduced by 17.24% (p <0.05), 20.11% (p <0.05) and 20.50% (p <0.05), respectively, compared with the control group; the redness a* of the experimental groups had a trend of improvement (0.05

0.05). In conclusion, dietary supplementation of different levels of garlic straw powder can improve meat quality and antioxidant capacity of yellow-feathered broilers without affecting growth performance and intestinal mucosal morphology.

Effects of a mixture of mono-glycerides of butyric-, capric-, and caprylic acid with chlortetracycline on the growth performance, intestine morphology, and cecal microflora of broiler birds.

This study aimed to investigate the effects of a mixture of mono-glycerides of butyric-, capric-, and caprylic acid (MMG) on the growth performance, intestinal morphology, and cecal microflora of broilers. A total of 960 male Arbor Acre broilers were offered basal diets with or without Chlortetracycline additive (CA) at 500 g/t, and MMG at 3,000, 1,000, or 650 g/t, with 8 replicates of 20 birds per treatment. The results confirmed 500 g/t CA with/without 1,000 g/t MMG increased the average daily weight gain (ADG) of birds compared to the control group 1 during the 42-d experimental period (P < 0.05). Comparing to the control group 1, 500 g/t CA with either 650 g or 1,000 g/t MMG or 1,000 g MMG alone increased the ADG of birds during the late growth stage (22-42 d) (P < 0.05). On d 42, the serum triglyceride levels were higher (P < 0.05) in groups supplemented with CA and CA + 1,000 g/t MMG comparing to the control group; while urea nitrogen level was higher in the control group comparing to the rest of treatment groups Compared to the control group 1, 1,000 g/t MMG alone without CA decreased the abundance of Faecalibacterium and Bacteroides but increased the abundance of Escherichia/Shiegella. About 500 g/t CA alone treatment group had higher abundance of Lactobacillus comparing to the rest of groups. In conclusion, dietary supplement with MMG showed beneficial efficacy on the growth and intestinal function of broilers, demonstrating the potential value of MMG to poultry industry. In terms of dosage, the current trial shows that 3,000 g/t (1-21 d) and 1,000 g/t (22-42 d) MMG without CA was the appropriate dietary supplemented rate for broilers. And the mixed use of 500 g/t CA and 1,000 g/t MMG was benefit for broilers at 22 to 42 d.

Magnolol additive improves growth performance of Linwu ducklings by modulating antioxidative status.

Magnolol is a bioactive polyphenolic compound commonly found in Magnolia officinalis. The aim of this study is to clarify the contribution of the magnolol additive on the growth performance of Linwu ducklings aging from 7 to 28 d, comparing to the effects of antibiotic additive (colistin sulphate). A total of 325, 7-d-old ducklings were assigned to 5 groups. Each group had 5 cages with 13 ducklings in each cage. The ducklings in different groups were fed with diets supplemented with 0, 100, 200 and 300 mg/kg magnolol additive (MA) (Control, MA100, MA200 and MA300) and 30 mg/kg colistin sulphate (CS30) for 3 weeks, respectively. Parameters regarding to the growth performance, intestinal mucosal morphology, serum biochemical indices, antioxidant and peroxide biomarkers and the expression levels of antioxidant-related genes were evaluated by one way ANOVA analysis. The results showed that 30 mg/kg colistin sulphate, 200 and 300 mg/kg magnolol additive improved the average final weight (P = 0.045), average daily body weight gain (P = 0.038) and feed/gain ratios (P = 0.001) compared to the control group. 200 and 300 mg/kg magnolol additive significantly increased the villus height/crypt depth ratio of ileum, compared to the control and CS30 groups (P = 0.001). Increased serum level of glucose (P = 0.011) and total protein (P = 0.006) were found in MA200 or MA300 group. In addition, comparing to the control and CS30 groups, MA200 or MA300 significantly increased the levels of superoxide dismutase (P = 0.038), glutathione peroxidase (P = 0.048) and reduced glutathione (P = 0.039) in serum. Moreover, the serum and hepatic levels of 8-hydroxy-2'-deoxyguanosine (P = 0.043 and 0.007, respectively) were lower in all MA groups compared to those of the control and CS30 group. The hepatic mRNA expression levels of superoxide dismutase-1, catalase and nuclear factor erythroid-2-related factor 2/erythroid-derived CNC-homology factor were also increased significantly in MA200 and MA300 groups (P < 0.05). Taken together, these data demonstrated that MA was an effective feed additive enhancing the growth performance of Linwu ducklings at 7 to 28 d by improving the antioxidant and intestinal mucosal status. It suggested that MA could be a potential ingredient to replace the colistin sulphate in diets.

Effects of Dietary Bacillus and Non-starch Polysaccharase on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Intestinal Morphology, and Serum Antioxidant Profiles in Ducks.

Given the desirable results of using probiotics and enzyme preparations as feed supplements in poultry health, here, the effects of Bacillus and Non-starch Polysaccharase (NSPase) on the growth performance, serum antioxidant profiles, and gut microbial communities of early stage ducks is investigated. A total of 400 Zhijiang ducks (of similar body weight and 1 day age) was selected and randomly divided into four groups. The feeding period was 28 days. Each group contained 10 replicates of 10 birds. Control group (I) was fed with basal diet, while treatment groups II to IV were fed, respectively, with 150 mg/kg NSPases, 25 mg/kg Bacillus probiotics, and 150 mg/kg NSPases + 25 mg/kg Bacillus probiotics in their basal diet. The results demonstrated that dietary Bacillus (25 mg/kg) increased average final weight, average daily gain (ADG), and decreased the malonaldehyde (MDA) in birds (P < 0.05). Dietary Bacillus (25 mg/kg) and NSPases + Bacillus (150 mg/kg + 25 mg/kg) presented much higher glutathione (GSH) and activities of superoxide dismutase (SOD) in birds (P < 0.05). Additionally, as revealed by ß-diversity indices and analysis of similarities, dietary NSPases + Bacillus could affect the ileum microbial abundances and diversities at the genera level (P < 0.05), but it had no effect on the caecal microbiota. Also, 16S rRNA sequencing revealed that dietary Bacillus and NSPases + Bacillus increased the populations of Ruminococcaceae genera in the cecum (P < 0.05), and S24-7_group and Lactobacillus genera in the ileum (P < 0.05). However, dietary NSPases and Bacillus alone and in combination could significantly decrease the content of Bacteroides in the ileum (P < 0.05). According to Spearman correlation analysis, 7 ilea bacterial microbiomes (S24-7 group, Lactobacillus, Subgroup 2, Subgroup 1, Kitasatospora, Candidatus Solibacter, and Akkermansia) were positively correlated with SOD (P < 0.05). In conclusion, Bacillus (25 mg/kg) and NSPases (150 mg/kg) included in the diet could efficiently enhance the growth performance by altered gut microbiota composition at the genera level and antioxidant indices of ducks.