RESUMO
The article presents data concerning analysis of bioassays under suspicion of influenza A(H1N1)pdm09. The technique of back transcription polymerase chain reaction and sequencing was applied. The separation of native influenza virus A was implemented using passaging through developing chick embryos.
Assuntos
Hemaglutininas Virais/genética , Vírus da Influenza A Subtipo H1N1/genética , Influenza Humana/virologia , RNA Viral/isolamento & purificação , Animais , Embrião de Galinha , Hemaglutininas Virais/isolamento & purificação , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Vírus da Influenza A Subtipo H1N1/patogenicidade , Vírus da Influenza A Subtipo H1N1/ultraestrutura , Influenza Humana/genética , Influenza Humana/patologia , Microscopia Eletrônica , RNA Viral/genéticaRESUMO
High in vitro and in vivo efficacy of Ingavirin against the Mexican pandemic influenza virus A/H1N1/2009, strains A/California/04/2009 (H1N1) and A/California/07/2009 (H1N1) vs. the reference drug Arbidol was studied and verified when used therapeutically and prophylactically.
Assuntos
Amidas/uso terapêutico , Antivirais/uso terapêutico , Ácidos Dicarboxílicos/uso terapêutico , Imidazóis/uso terapêutico , Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos , Influenza Humana , Caproatos , Humanos , Indóis/uso terapêutico , Influenza Humana/tratamento farmacológico , Influenza Humana/prevenção & controleRESUMO
Ingavirin was shown to be efficient in inhibition of the influenza virus strains A/California/04/2009 and A/California/07/2009 (H1N1) in the MDCK cell culture. The coefficient of the cytopathic activity inhibition amounted to 50 and 60%, and the inhibition coefficients of the specific hemagglutinin formation were 50 and 50%, and 79,1 and 75% before and after the enrichment in chick embryos respectively.
