RESUMO
The presence of virus DNA integration into the cell genome was studied for 47 primary HPV16-positive patients with morphologically verified stage III cervical cancer. By using ROC analysis, the patients were divided into two groups: with and without HPV DNA integration into the host cell genome. The differences between the groups by the histological type, degree of tumor differentiation, and primary response to therapy were statistically insignificant. Virus DNA integration more than 7-fold reduced 5-year relapse-free survival and 1.7-fold reduced overall survival rate in comparison with patients without HPV DNA integration (p=0.0002 and p=0.05, respectively). The relative risk of adverse outcome of the disease in patients with the presence of HPV16 DNA integration increases by 4 times over a period of less than 3 years (Ñ=0.0006) at high AUC level. The probability of earlier progression of the disease in patients with of HPV DNA integration calculated according to the Cox proportional hazards model was 85.5% (hazard ratio 5.96; p=0.002). Thus, the results suggest that the presence of HPV16 DNA integration into the cell genome is an independent factor in predicting clinical outcome of advanced cervical cancer and can serve as an effective criterion for the individual choice of treatment tactics for the patients.
Assuntos
DNA Viral/genética , Papillomaviridae/genética , Papillomaviridae/patogenicidade , Infecções por Papillomavirus/genética , Neoplasias do Colo do Útero/patologia , Feminino , Humanos , Recidiva Local de Neoplasia/genética , Prognóstico , Modelos de Riscos Proporcionais , Neoplasias do Colo do Útero/genética , Neoplasias do Colo do Útero/virologia , Integração Viral/genética , Integração Viral/fisiologiaRESUMO
Complex treatment with acetylsalicylic acid, heparin, and dexamethasone improves the efficiency of photodynamic therapy in rats with myosarcoma-I.
Assuntos
Hemostasia , Neoplasias Experimentais/terapia , Fotoquimioterapia , Animais , Feminino , Ratos , Ratos WistarRESUMO
Natural latent human antibodies cross-reacting with DNA and cardiolipin, interact with human endothelial cells, decrease antiaggregation activity of rat aortic wall, and increase fibrinolytic activity of the wall of the inferior vena cava. It is assumed that natural antiphospholipid antibodies present in immunoglobulin preparations in a latent state modify antithrombogenic activity of the vascular wall and are a potential cause of antiphospholipid syndrome.