Performance of microscopic observation drug susceptibility for the rapid diagnosis of tuberculosis and detection of drug resistance in Bamako, Mali.

OBJECTIVES: In Mali early detection and treatment of multidrug-resistant tuberculosis (MDR-TB) are still challenging due to the cost, time and/or complexity associated with regular tests. Microscopic Observation Drug Susceptibility (MODS) is a low-cost assay validated by WHO in 2010. It is a liquid-culture-based assay to detect the 'cording' characteristic of Mycobacterium tuberculosis complex and to assess susceptibility to both isoniazid and rifampicin defining multidrug-resistant tuberculosis (MDR-TB). In this study we aimed to evaluate the performance of MODS as diagnostic tool compared with a validated method-Mycobacteria Growth Indicator Tube/Antimicrobial Susceptibility Testing/Streptomycin, Isoniazid, Rifampicin and Ethambutol (MGIT/AST/SIRE). METHODS AND RESULTS: Between January 2010 and October 2015 we included 98 patients with suspected TB in an observational cohort study. The sensitivity and specificity of MODS assay for detecting TB were respectively 94.12% and 85.71% compared with the reference MGIT/7H11 culture, with a Cohen κ coefficient of 0.78 (95% CI 0.517-1.043). The median time to culture positivity for MODS assay and MGIT (plus interquartile range, IQR) was respectively 8 days (IQR 5-11) and 6 days (IQR 5-6). In detecting patients with MDR-TB, the sensitivity and specificity of MODS assay were respectively 100% and 95.92%. The positive predictive value and negative predictive value were, respectively, 66.7% and 100%. The median turnaround times for obtaining MDR-TB results using MODS assay and MGIT/AST/SIRE was respectively 9 days and 35 days. Hence, the MODS assay rapidly identifies MDR-TB in Mali compared with the MGIT/AST/SIRE. CONCLUSION: As an easy, simple, fast and affordable method, the MODS assay could significantly improve the management of TB.

Tuberculosis drug resistance in Bamako, Mali, from 2006 to 2014.

BACKGROUND: Although Drug resistance tuberculosis is not a new phenomenon, Mali remains one of the "blank" countries without systematic data. METHODS: Between 2006 and 2014, we enrolled pulmonary TB patients from local TB diagnostics centers and a university referral hospital in several observational cohort studies. These consecutive patients had first line drug susceptibility testing (DST) performed on their isolates. A subset of MDR was subsequently tested for second line drug resistance. RESULTS: A total of 1186 mycobacterial cultures were performed on samples from 522 patients, including 1105 sputa and 81 blood samples, yielding one or more Mycobacterium tuberculosis complex (Mtbc) positive cultures for 343 patients. Phenotypic DST was performed on 337 (98.3%) unique Mtbc isolates, of which 127 (37.7%) were resistant to at least one drug, including 75 (22.3%) with multidrug resistance (MDR). The overall prevalence of MDR-TB was 3.4% among new patients and 66.3% among retreatment patients. Second line DST was available for 38 (50.7%) of MDR patients and seven (18.4%) had resistance to either fluoroquinolones or second-line injectable drugs. CONCLUSION: The drug resistance levels, including MDR, found in this study are relatively high, likely related to the selected referral population. While worrisome, the numbers remained stable over the study period. These findings prompt a nationwide drug resistance survey, as well as continuous surveillance of all retreatment patients, which will provide more accurate results on countrywide drug resistance rates and ensure that MDR patients access appropriate second line treatment.

Molecular strain typing of Mycobacterium tuberculosis complex in Bamako, Mali.

OBJECTIVE: To identify strains of Mycobacterium tuberculosis complex (MTC) circulating in Bamako and to examine the relationship between the strains and their drug susceptibility profiles. METHODS: Between 2006 and 2010, we conducted a cross-sectional study using spoligotyping to identify strains of MTC recovered from 126 tuberculosis (TB) patients under treatment in Bamako, Mali. RESULT: Three members of the MTC were isolated: M. tuberculosis (71.4%), M. africanum (27.8%) and M. bovis (0.8%). Of these, three strains were found to be the most prevalent: M. tuberculosis T1 (MTB T1; 38.9%), M. africanum F2 (MAF2; 26.2%) and M. tuberculosis Latin American and Mediterranean 10 (MTB LAM 10; 10.3%). MAF2 and MTB LAM 10 strains have a lower risk of multidrug resistance (MDR) than MTB T1 (respectively OR 0.1, 95%CI 0.03-0.4 and OR 0.1, 95%CI 0.01-0.8). Age ≥ 32 years (OR 1.4, 95%CI 0.4-3.9), negative human immunodeficiency virus status (OR 0.4, 95%CI 0.1-2.5) and male sex (OR 4, 95%CI 0.9-16.5) were not associated with MDR. The prevalence of MDR among treatment and retreatment failure patients was respectively 25% and 81.8% compared to new patients (2.9%). CONCLUSION: This study indicates a low level of primary drug resistance in Bamako, affirms the importance of using correct drug regimens, and suggests that the MTB T1 strain may be associated with the development of resistance.

[Knowledge of the Bamako general population of tuberculosis]. / Connaissance de la population generale de Bamako sur la tuberculose.

The hope of the World Health Organization (WHO) in the fight against tuberculosis rests today on the implementation of the strategy DOTS. The success of this strategy passes obligatorily by an implication of the parents, neighbors in one word of the population living with the patient; this is why we fixed our objective to study knowledge on the tuberculosis of the people of more than 18 years of the district of Bamako. We carried out a cross-sectional study supplemented by focus-groups in 3 districts of Bamako near the general population (socio-medical personnel, old tuberculous, and helping natural) for the period from the 1st of June to July 15, 2004. Our sample was composed of 246 people for the individual questionnaires and of 47 per 8 meetings of focus group: the sex ratio was 2,5 in favour of the men and 60,2% of our subjects had less than 30 years. In the general population of Bamako 24,8% had a good knowledge, 49,0% an average knowledge and 26,2% a bad knowledge. 48,8 % of those which had a good knowledge were the pupils, students and civils servant. So in general the population had a good knowledge on symptomatology (90,2 %), it on the other hand had the knowledge very limited on the etiology (only 10,6% of the subject knew that tuberculosis is caused by a mycobactery) or on the modes of transmission (cigarettes, soap, meat). The population of Bamako has a very passable level of knowledge on tuberculosis. If this level is good with regard to symptomatology, it is very insufficient on the etiology or on the transmission of the disease. To improve this knowledge one needs a sensitizing supported for television and the radio in the dialects and national languages.

[The socio-economic impact of asthmatic disease at Bamako about 131 cases]. / Impact socio-economique de la maladie asthmatique a Bamako a propos de 131 cas.

With an aim of evaluating the socio-economic impact of the asthmatic disease in Mali, a cross-sectional study was carried out of July 1st to September 30, 2006 in the district of Bamako. The study proceeded with the service of Pneumo-phthisiology of the CHU of the point G and in 4 of the 6 drawn health center of reference of the district to the fate. 131 asthmatic patients were indexed on 1595 consultations is a frequency of 8, 24%. The average age was 31 years, the sex-ratio was of 1,2 in favour of the men. August recorded the greatest number of consultation is 50.4%. The antecedent of family asthma was found in 46, 6% of the cases, the nicotinism in 13% of the cases. The average therapeutic cost per annum rose of 75.750 is 115, 47 EUROS without hospitalization with 222.750 FCFA (339, 57 EUROS) FCFA with hospitalization. The hospital expenses rose on average to. 147000 FCFA (224, 09 EUROS) that is to say 66, 21% of the costs of assumption of responsibility. The intermediate duration of hospitalization was 5,5 days 1 to 4 days without activity was noted at 50.4% of the cases, on average 312 days of absence. The professional damage was announced in 13, 7%. The economic burden was supported by the father in 29% of the cases. In 42% of the cases in charge one of expenditure was civil servant. Asthma is a social disease from its impact on the family economy.

Impact socio-économique de la maladie asthmatique à Bamako à propos de 131 cas

Dans le but d'évaluer l'impact socio-économique de la maladie asthmatique au Mali, une étude transversale a été réalisée du 1er juillet au 30 septembre 2006 dans le district de Bamako. L'étude s'est déroulée au service de Pneumo-phtisiologie du CHU du point G et dans 4 des 6 centres de santé de référence du district tirés au sort. 131 malades asthmatiques ont été répertoriés sur 1595 consultations soit une fréquence de 8,24%. La moyenne d'âge était de 31 ans, le sex-ratio était de 1,2 en faveur des hommes. Le mois d'Août a enregistré le plus grand nombre de consultation soit 50,4%. L'antécédent d'asthme familial a été retrouvé dans 46,6% des cas, le tabagisme dans 13% des cas. Le coût thérapeutique moyen par an s'élevait de 75.750 FCFA soit 115,47 EUROS sans hospitalisation à 222.750 FCFA (339,57 EUROS) avec hospitalisation. Les frais d'hospitalisations s'élevaient en moyenne à 147.000 FCFA (224,09 EUROS) soit 66,21% des coûts de prise en charge. La durée moyenne d'hospitalisation était de 5,5 jours1 à 4 jours sans activité ont été notés chez 50,4% des cas, en moyenne 312 jours d'absence. Le dommage professionnel a été signalé dans 13,7%. Le fardeau économique était supporté par le père dans 29% des cas. Dans 42% des cas le chargé de dépense était fonctionnaire

[Etiology of pleurisy in the Service of Pneumology, Point G]. / Etiologies des pleuresies dans le service de pneumologie du point G.

This study describes epidemiologic, etiologic and evaluative aspects of Pleurisy pneumology department of Point "G". From 1st January 1998 to 31 December 2002 was realised a study about hospitalized patients in the department. Pleurisy represented 15; 9% of hospitalizations. The sex Ratio was 2,3 en for men aged of 41 years 55 of pleurisy were located on right. In 60% of cases the liquid was sero-fibrin, purulent in 25% of cases and haemorrhagic in 15%. Tuberculosis was the most frequent etiology with 37% of cases. According to liquid aspect tuberculosis represented 54, 3% of sero-fibrin pleurisy in one hand, on the other 60% of haemorrhagic pleurisy was due to cancer. The serology HIV have been realised on 89 patients and it was positive on 56% of cases. Association pleurisy/HIV tuberculosis etiology represented 56% of cases. Among 369 patients we have counted 100 deaths, 27% and 86% of these death occurred before a month of hospitalization. Even if the prevalence of neoplastic pleurisy increases, tuberculosis still the 1st etiology of pleurisy. It co-infection with HIV is the principal cause of morbidity and mortality.

[Problems with lung cancer in the pneumology service at G Barnako Hospital, Mali]. / Problematique du cancer bronchique dans leservice de pneumologie du point G Bamako Mali.

The authors report the results of a retrospective study on the bronchial cancers observed in the service of PPH of the hospital of the point G of Bamako during the time active of January 1, 1999 to December 31, 2003. The goal was to describe the epidemiological, clinical and therapeutic aspects of bronchial cancer On 669 in-patients, 36 cases of bronchial cancer were diagnosed either a prevalence of 5.36%. the average age of the patients was 54.5 years with the extreme ones going from 35 to 80 years. Our series was made up of 27 men and 9 women with a sex ratio of 3 in favor of the men. The nicotinic was the principal factor of risk (69.4%) with an average of 20,9 packages year. The mode of prevalent nicotinic was the cigarette (92%). The functional signs most frequent were the thoracic pain (72.2%); cough (56.6%); dyspnea (22.2%). The histological type was found in 19.4% of the cases, carcinomas épidermoíde were more frequents (42.9%). 93.4% of the patients had profited only from one palliative treatment. Bronchial cancer poses enormous problems with the experts of the countries under equipped. Its appalling forecast is related to the delays and insufficiencies diagnostic and therapeutic

[Contribution of radiography and repeat microscopy in the diagnosis of pulmonary tuberculosis in the hospital environment of Bamako]. / Apport de la radiographie et de la bacilloscopie répétée dans le diagnostic de la tuberculose pulmonaire en milieu hospitalier à Bamako.

WHO and IUAT-LD recommend repeated microscopy in order to improve the detection of contagious cases of pulmonary tuberculosis. Our aim was to determine the contributions of radiology and microscopy in the diagnosis of pulmonary tuberculosis. From January 1998 to April 1999 radiography and microscopy were performed for 275 patients who were admitted to the pneumology service. Pulmonary tuberculosis prevalence was higher among women aged 10 to 29 years than among men of the same age (p = 10(-6)). 188 (68.4%) women tested positive, 49 (17.8%) tested positive at the second microscopy and 1 (0.36%) at the the third. The main radiological lesions were nodules + infiltrats (46.2%), nodules + infiltrats + cavity (26.2%) and infiltrats + cavity (6.5%). Repeated microscopy significantly increased the proportion of pulmonary tuberculosis cases detected (from a prevalence 68.4% to 86.5%).

[Epidemiological, clinical and progressive aspects of asthma observed at Bamako, Mali]. / Aspects épidémiologiques, cliniques et évolutifs de l'asthme observés à Bamako, Mali.

We carried out a prospective study of 106 of the 752 asthmatic patients attending the outpatient clinic of Point G Hospital, Bamako, between December 1997 and November 1998. We analyzed data for 61 patients whose lung function was assessed by measurement of peak expiratory flow. The frequency of asthma was 14.9%, suggesting regular progression of the disease. There were slightly more women than men (55.7% women) in the study population and the mean age of the patients was 31 years. Most of the patients (65.5%) were recruited in the dry season (November to May). Based on peak expiratory flow measurements, intermittent asthma was the most frequent type of asthma observed (37.8%), followed by moderate persistent asthma (34.4%), mild persistent asthma (18%) and severe persistent asthma (9.8%). The mean cost per episode of asthma was $36 (US) and the mean cost per patient was $432 (US) per year. In 52.45% of cases, the drugs required were available and the patient had access to them. They were available but not accessible in 26.22% of cases. To improve the management of asthma in countries with limited financial resources, generic drugs should be made available.