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1.
Food Chem ; 266: 275-283, 2018 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-30381186

RESUMO

The six biogenic amines in sausage and cheese were analyzed by HPLC with UV detection after off-line derivatization with dansyl chloride, 9-fluorenylmethoxycarbonyl chloride, benzoyl chloride and dabsyl chloride, respectively. The results showed that both the off-line 9-fluorenylmethoxycarbonyl and dabsyl derivatization were not suitable for HPLC analysis of biogenic amines when batch injection was used because the derivatives were instable, whereas both the off-line dansyl and benzoyl derivatization were suitable for HPLC analysis of biogenic amines when batch injection was used, but the latter needed to maintain the derivatives at 4 °C to ensure that benzoylated tyramine was not degraded when waiting for the analysis. The off-line dansyl derivatization had an obvious advantage in the analysis of biogenic amines in sausage and cheese samples by HPLC combined with batch injection because the method has a wider linear range and higher sensitivity, accuracy, precision and stability of the derivatives.


Assuntos
Aminas Biogênicas/análise , Queijo/análise , Cromatografia Líquida de Alta Pressão/métodos , Produtos da Carne/análise , Benzoatos/química , Aminas Biogênicas/química , Aminas Biogênicas/isolamento & purificação , Compostos de Dansil/química , Análise de Alimentos , Extração em Fase Sólida , Espectrofotometria Ultravioleta/métodos
2.
Zhonghua Bing Li Xue Za Zhi ; 38(7): 441-4, 2009 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-19781189

RESUMO

OBJECTIVE: To study the distribution and quantity of CD44+/CD24- cells in breast cancer tissue and the cell lines, and as well as its correlation with the expression of various breast cancer markers and molecular subtyping of breast carcinoma. METHODS: The expression of CD44/CD24, estrogen receptor, progesterone receptor, HER2, human estrogen-induced protein PS2, bcl-2 and nm23 in 60 cases of invasive ductal carcinoma of breast were studied by either single or double immunohistochemical staining. The co-expression of CD44 and CD24 in 3 breast cancer cell lines (MCF-7, MDA-MB-468, and MDA-MB-231) was also examined. RESULTS: The quantity and distribution of CD44+/CD24- cells varied greatly and no specific patterns were identified. The percentage of CD44+/CD24- in breast cancer was 65%. The amount of CD44+/CD24- cells did not correlate with the age of patients, lymph node metastasis, tumor size, molecular subtypes and expression of various breast cancer markers in breast carcinoma. The proportion of CD44+/CD24- cells in MCF-7, MDA-MB-468, and MDA-MB-231 cell lines was <1%, 5% and >80%, respectively. CONCLUSIONS: CD44+/CD24- cells are demonstrated in certain breast cancer tissues and cell lines. However, there is no relationship obtained between the quantity or the distribution of these cells and the molecular subtyping or the clinicopathologic parameters in breast cancer.


Assuntos
Neoplasias da Mama/patologia , Antígeno CD24/metabolismo , Carcinoma Ductal de Mama/patologia , Receptores de Hialuronatos/metabolismo , Adulto , Idoso , Biomarcadores Tumorais , Neoplasias da Mama/classificação , Neoplasias da Mama/metabolismo , Carcinoma Ductal de Mama/classificação , Carcinoma Ductal de Mama/metabolismo , Linhagem Celular Tumoral , Feminino , Humanos , Metástase Linfática , Pessoa de Meia-Idade , Nucleosídeo NM23 Difosfato Quinases/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Receptor ErbB-2/metabolismo , Receptores de Progesterona/metabolismo , Fator Trefoil-1 , Proteínas Supressoras de Tumor/metabolismo
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