Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Int J Mol Sci ; 16(3): 4628-41, 2015 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-25739079

RESUMO

Rice blast is a major destructive fungal disease that poses a serious threat to rice production and the improvement of blast resistance is critical to rice breeding. The antimicrobial peptide MSI-99 has been suggested as an antimicrobial peptide conferring resistance to bacterial and fungal diseases. Here, a vector harboring the MSI-99 gene was constructed and introduced into the tobacco chloroplast genome via particle bombardment. Transformed plants were obtained and verified to be homoplastomic by PCR and Southern hybridization. In planta assays demonstrated that the transgenic tobacco plants displayed an enhanced resistance to the fungal disease. The evaluation of the antimicrobial activity revealed that the crude protein extracts from the transgenic plants manifested an antimicrobial activity against E. coli, even after incubation at 120 °C for 20 min, indicating significant heat stability of MSI-99. More importantly, the MSI-99-containing protein extracts were firstly proved in vitro and in vivo to display significant suppressive effects on two rice blast isolates. These findings provide a strong basis for the development of new biopesticides to combat rice blast.


Assuntos
Peptídeos Catiônicos Antimicrobianos/genética , Cloroplastos/genética , Resistência à Doença/genética , Peptídeos/genética , Alternaria/efeitos dos fármacos , Alternaria/fisiologia , Peptídeos Catiônicos Antimicrobianos/metabolismo , Peptídeos Catiônicos Antimicrobianos/farmacologia , Western Blotting , Cloroplastos/metabolismo , Escherichia coli/efeitos dos fármacos , Interações Hospedeiro-Patógeno/efeitos dos fármacos , Magnaporthe/fisiologia , Microscopia Confocal , Oryza/microbiologia , Peptídeos/metabolismo , Peptídeos/farmacologia , Doenças das Plantas/genética , Doenças das Plantas/microbiologia , Folhas de Planta/microbiologia , Plantas Geneticamente Modificadas , Protoplastos/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/farmacologia , Nicotiana/genética , Nicotiana/metabolismo , Nicotiana/microbiologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA