[Pericardial and myocardial involvement after SARS-CoV-2 infection: a cross-sectional descriptive study in healthcare workers]. / Afección pericárdica y miocárdica tras infección por SARS-CoV-2: estudio descriptivo transversal en trabajadores sanitarios.

Introduction and objectives: The cardiac sequelae of SARS-CoV-2 infection are still poorly documented. We conducted a cross-sectional study in healthcare workers to report evidence of pericardial and myocardial involvement after SARS-CoV-2 infection. Methods: We studied 139 healthcare workers with confirmed past SARS-CoV-2 infection. Participants underwent clinical assessment, electrocardiography, and laboratory tests, including immune cell profiling and cardiac magnetic resonance (CMR). Clinically suspected pericarditis was diagnosed when classic criteria were present and clinically suspected myocarditis was based on the combination of at least 2 CMR criteria. Results: Median age was 52 (41-57) years, 71.9% were women, and 16.5% were previously hospitalized for COVID-19 pneumonia. On examination (10.4 [9.3-11.0] weeks after infection-like symptoms), participants showed hemodynamic stability. Chest pain, dyspnea or palpitations were present in 41.7% participants, electrocardiographic abnormalities in 49.6%, NT-proBNP elevation in 7.9%, troponin in 0.7%, and CMR abnormalities in 60.4%. A total of 30.9% participants met criteria for either pericarditis and/or myocarditis: isolated pericarditis was diagnosed in 5.8%, myopericarditis in 7.9%, and isolated myocarditis in 17.3%. Most participants (73.2%) showed altered immune cell counts in blood, particularly decreased eosinophil (27.3%; P < .001) and increased cytotoxic T cell numbers (17.3%; P < .001). Clinically suspected pericarditis was associated (P < .005) with particularly elevated cytotoxic T cells and decreased eosinophil counts, while participants diagnosed with clinically suspected myopericarditis or myocarditis had lower (P < .05) neutrophil counts, natural killer-cells, and plasma cells. Conclusions: Pericardial and myocardial involvement with clinical stability are frequent after SARS-CoV-2 infection and are associated with specific immune cell profiles.Full English text available from:www.revespcardiol.org/en.

Pericardial and myocardial involvement after SARS-CoV-2 infection: a cross-sectional descriptive study in healthcare workers.

INTRODUCTION AND OBJECTIVES: The cardiac sequelae of SARS-CoV-2 infection are still poorly documented. We conducted a cross-sectional study in healthcare workers to report evidence of pericardial and myocardial involvement after SARS-CoV-2 infection. METHODS: We studied 139 healthcare workers with confirmed past SARS-CoV-2 infection. Participants underwent clinical assessment, electrocardiography, and laboratory tests, including immune cell profiling and cardiac magnetic resonance (CMR). Clinically suspected pericarditis was diagnosed when classic criteria were present and clinically suspected myocarditis was based on the combination of at least 2 CMR criteria. RESULTS: Median age was 52 (41-57) years, 71.9% were women, and 16.5% were previously hospitalized for COVID-19 pneumonia. On examination (10.4 [9.3-11.0] weeks after infection-like symptoms), participants showed hemodynamic stability. Chest pain, dyspnea or palpitations were present in 41.7% participants, electrocardiographic abnormalities in 49.6%, NT-proBNP elevation in 7.9%, troponin in 0.7%, and CMR abnormalities in 60.4%. A total of 30.9% participants met criteria for either pericarditis and/or myocarditis: isolated pericarditis was diagnosed in 5.8%, myopericarditis in 7.9%, and isolated myocarditis in 17.3%. Most participants (73.2%) showed altered immune cell counts in blood, particularly decreased eosinophil (27.3%; P<.001) and increased cytotoxic T cell numbers (17.3%; P <.001). Clinically suspected pericarditis was associated (P <.005) with particularly elevated cytotoxic T cells and decreased eosinophil counts, while participants diagnosed with clinically suspected myopericarditis or myocarditis had lower (P <.05) neutrophil counts, natural killer-cells, and plasma cells. CONCLUSIONS: Pericardial and myocardial involvement with clinical stability are frequent after SARS-CoV-2 infection and are associated with specific immune cell profiles.

Iron coupling with carbon fiber to stimulate biofilms formation in aerobic biological film systems for improved decentralized wastewater treatment: Performance, mechanisms and implications.

Iron coupling with carbon fiber (ICF) as carriers to stimulate the biofilms formation for decentralized wastewater treatment was proposed. The typical pollutants removal was accelerated and enhanced (increased by 13.65% for chemical oxygen demand, 19.68% for ammonia nitrogen and 32.66% for phosphate) in ICF compared with the traditional carbon fiber (CF) system. Mechanism explorations indicated that the iron coupling improved the surface properties of carbon fibers and contributed to the attachment and growth of biomass significantly. The components of biomass were changed with increasing proteins proportion in ICF, which was beneficial to the biofilms formation and stability. The microbial community was altered with the enrichment of functional microorganisms (i.e. Pseudomonas and Thauera). Moreover, the microbial metabolic functions (i.e. enzymatic activities and encoding genes) involved in pollutants removal derived from decentralized wastewater were highly expressed in ICF. This work provided an effective strategy to enhance the decentralized wastewater treatment.

Pacientes con criterios clínicos de síndrome de Lynch con y sin mutación en genes reparadores del ADN ( MLH1 y MSH2). Un reto para el clínico / Patients with clinical criteria for Lynch syndrome with and without mutations in DNA repair genes ( MLH1 and MSH2). A challenge for the clinician

Fundamento y objetivo: El objetivo del estudio es conocer las características clínico-patológicas de los pacientes diagnosticados de cáncer colorrectal (CCR) con criterios clínicos de síndrome de Lynch, en nuestro medio, con el fin de valorar y mejorar la atención de los mismos y de sus familias, a través de la Unidad de Consejo Genético de nuestro centro.Pacientes y método: Se trata de un estudio con diseño observacional, transversal y con recogida de datos retrospectiva. La muestra objeto de estudio está constituida por todos los pacientes con criterios clínicos de síndrome de Lynch a los que se les realizó análisis molecular, a través de la Unidad de Consejo Genético de Salamanca, en el período 2004-2009. Se incluyeron variables relacionadas con el paciente, con el tumor, así como la presencia o ausencia de mutación en MLH1 y MSH2. Resultados: Se estudiaron un total de 76 pacientes, 15 de los cuales presentaban mutación, bien en MLH1 bien en MSH2. La edad media al diagnóstico del cáncer colorrectal fue de 51,2 y 54,3 años en el grupo sin y con mutación, respectivamente, con una distribución por sexos similar en ambos grupos. Se ha observado una amplia heterogeneidad fenotípica en la muestra analizada.Conclusiones: El síndrome de Lynch es una entidad difícil de categorizar desde un punto de vista clínico. Por lo tanto, es importante estar alerta para un mejor manejo de estos pacientes y de sus familias (AU)

Background and objectives: The objective was to study the clinicopathologic characteristics of patients diagnosed of colorectal cancer (CRC) with clinical criteria for Lynch syndrome, in our region, in order to assess and improve the care of them and their families in the Genetic Counseling Unit of our hospital. Patients and methods: This was an observational, transversal retrospective study. The studied sample was made up of all the patients with clinical criteria for Lynch syndrome, who underwent a molecular analysis test in the Genetic Counseling Unit of Salamanca, during the period 2004-2009. We included patient and tumor related variables and the presence or absence of mutations in MLH1 and MSH2. Results: A total of 76 patients were included in the analysis. Fifteen of them carried a mutation either in MLH1 or in MSH2. The mean age at diagnosis of colorectal cancer was 51.2 and 54.3 years in the group with and without mutation respectively, with a similar gender distribution in both groups. A wide phenotypic heterogeneity was found in the sample. Conclusions: Lynch syndrome is an entity difficult to categorize from a clinical point of view. Therefore, it is important to be alert for a better management of these patients and their families (AU)

[Patients with clinical criteria for Lynch syndrome with and without mutations in DNA repair genes (MLH1 and MSH2). A challenge for the clinician]. / Pacientes con criterios clínicos de síndrome de Lynch con y sin mutación en genes reparadores del ADN (MLH1 y MSH2). Un reto para el clínico.

BACKGROUND AND OBJECTIVES: The objective was to study the clinicopathologic characteristics of patients diagnosed of colorectal cancer (CRC) with clinical criteria for Lynch syndrome, in our region, in order to assess and improve the care of them and their families in the Genetic Counseling Unit of our hospital. PATIENTS AND METHODS: This was an observational, transversal retrospective study. The studied sample was made up of all the patients with clinical criteria for Lynch syndrome, who underwent a molecular analysis test in the Genetic Counseling Unit of Salamanca, during the period 2004-2009. We included patient and tumor related variables and the presence or absence of mutations in MLH1 and MSH2. RESULTS: A total of 76 patients were included in the analysis. Fifteen of them carried a mutation either in MLH1 or in MSH2. The mean age at diagnosis of colorectal cancer was 51.2 and 54.3 years in the group with and without mutation respectively, with a similar gender distribution in both groups. A wide phenotypic heterogeneity was found in the sample. CONCLUSIONS: Lynch syndrome is an entity difficult to categorize from a clinical point of view. Therefore, it is important to be alert for a better management of these patients and their families.