Transgenic, high-protein sorghums display promise in poultry diets in an initial comparison.

This study aimed to compare the inclusion of transgenic sorghums against commercially available sorghums on growth performance in broiler chickens. Isonitrogenous and isoenergetic diets were offered to a total 288 male Ross 308 broiler chickens from 14 to 35 d posthatch. Three dietary treatments were diets based on transgenic sorghums with a mean protein content of 154.7 g/kg and 5 treatments were based on commercially available sorghum hybrids with a mean protein content of 90.6 g/kg. Soybean meal inclusions in the commercial sorghum diets averaged 215 g/kg, which was reduced to 171 g/kg in the transgenic sorghum diets because of the higher protein contents. Overall growth performance was highly satisfactory, and commercial sorghums supported 2.55% (2,330 vs. 2,272 g/bird; P = 0.010) more weight gains and 2.74% (2,929 vs. 2,851 g/bird; P = 0.012) higher feed intakes; however, the transgenic sorghums supported a fractionally better FCR (1.255 vs 1.257; P = 0.826). There were no statistical differences in apparent jejunal and ileal starch and protein (N) digestibility coefficients between treatments. The transgenic sorghum diets generated slightly, but significantly, higher AME:GE ratios and AMEn, but the commercial sorghum diets generated 6.33% (235 vs. 221 g/kg; P < 0.001) greater breast meat yields. Apparent ileal digestibility coefficients of 16 amino acids averaged 0.839 and 0.832 for transgenic and commercial sorghum-based diets, respectively, without any significant differences in individual amino acids. This outcome suggests amino acid digestibilities of the transgenic sorghums may be inherently higher than commercial hybrid sorghums as the 25.7% higher average soybean meal inclusions would have advantaged amino acid digestibilities in commercial sorghum diets. The possibility that the digestibilities of amino acids in the kafirin component of transgenic sorghums was enhanced by modifications to the structure of kafirin protein bodies is discussed. In conclusion, transgenic sorghums with higher protein concentrations led to 20.5% reduction of soybean meal inclusions in broiler diets, and this change did not compromise feed conversion efficiency compared to standard commercial hybrid sorghums.

The influence of non-bound amino acid inclusions and starch-protein digestive dynamics on growth performance of broiler chickens offered wheat-based diets with two crude protein concentrations.

The primary objective of this study was to investigate the influence of high and low inclusions of non-bound amino acid (NBAA) in standard and reduced-crude protein (CP), wheat-based diets on growth performance in broiler chickens. Dietary treatments were formulated to either 210 or 180 g/kg CP. The 210 g/kg CP diets contained either 12.1 or 21.1 g/kg NBAA and 180 g/kg CP diets contained either 44.0 or 55.5 g/kg NBAA. The formulations also generated different dietary starch:protein ratios which impacted on starch-protein digestive dynamics. Each of the four dietary treatments were offered to 7 replicates of 15 birds housed in floor pens from 14 to 35 days post-hatch or a total of 420 male Ross 308 chickens. Growth performance, relative abdominal fat-pad weights, breast muscle and leg shank yields were determined. Ileal starch and protein (N) digestibility coefficients, disappearance rates and starch:protein disappearance rate ratios were defined. Apparent ileal digestibility coefficients and disappearance rates of 16 amino acids were determined at 35 days post-hatch and free concentrations of 20 amino acids in systemic plasma were determined at 34 days post-hatch. The transition from 210 to 180 g/kg CP diets depressed weight gain by 11.3% (1742 versus 1964 g/bird) and FCR by 10.4% (1.606 versus 1.455), although both parameters were subject to treatment interactions. The treatment interaction (P < 0.001) observed for FCR was because high NBAA inclusions significantly improved FCR by 4.17% (1.424 versus 1.486) in birds offered 210 g/kg CP diets, but significantly depressed FCR by 3.36% (1.632 versus 1.579) in 180 g/kg CP diets. A quadratic relationship (r = 0.860; P < 0.001) between dietary NBAA inclusions and FCR was detected, which indicated that when NBAA inclusions exceed 18.5 g/kg efficiency of feed conversion deteriorated. However, a multiple linear regression (r = 0.913; P < 0.001) was detected for FCR where both NBAA inclusions and analysed dietary starch:protein ratios were significantly (P < 0.001) related to FCR. This relationship indicates that growth performance of broiler chickens offered wheat-based diets is strongly influenced by dietary NBAA inclusions coupled with dietary starch:protein ratios and consideration is given to the possible underlying mechanisms.

The influence of dietary crude protein concentrations, grain types and arginine:lysine ratios on the performance of broiler chickens.

The objective of this study was to investigate the effects of dietary crude protein (CP) concentrations, grain types and arginine:lysine ratios on performance parameters of broiler chickens. The 2 × 2 × 2 factorial array of dietary treatments harnessed two CP concentrations (210 and 170 g/kg), two feed grains (wheat and sorghum), and two arginine:lysine ratios (104 and 110). Each dietary treatment was offered to 7 replicates of 14 birds per floor pen, a total of 784 off-sex male, Ross 308 broilers, from 14 to 35 d post-hatch. The dietary CP reduction compromised weight gain by 10.0% (2078 versus 2310 g/bird) as a main effect and FCR by 7.51% (1.474 versus 1.371), subject to an interaction. In a three-way interaction (P = 0.008), expanded arginine:lysine ratios improved FCR by 2.30% in 170 g/kg CP, sorghum-based diets but compromised FCR by 2.12% in corresponding wheat-based diets. Sorghum was the more suitable feed grain in reduced-CP diets as sorghum generated significant advantages in weight gain of 7.59% (2154 versus 2002 g/kg) and FCR of 6.94% (1.421 versus 1.527) in birds offered 170 g/kg CP diets. Both dietary CP and feed grain generated significant and divergent impacts in apparent ileal digestibility coefficients for the majority of 16 assessed amino acids. Dietary CP reductions increased non-bound amino acid inclusions (NBAA) in wheat-based diets (48.96 versus 9.80 g/kg) to a greater extent than sorghum-based diets (35.3 versus 9.50 g/kg) and increasing dietary NBAA inclusions were linearly associated with compromised weight gain (r = -0.834; P < 0.001) and FCR (r = 0.862; P < 0.001). Increasing ratios of free arginine to lysine plasma concentrations were linearly (r = -0.466; P = 0.004) related to improvements in FCR. The implications of the observed outcomes are discussed and possible explanations are advanced.

Dietary crude protein reductions in wheat-based diets with two energy densities compromised performance of broiler chickens from 15 to 36 days post-hatch.

This study was designed to investigate the impacts of 2 energy densities (13.0 and 12.5 MJ/kg ME) in wheat-based diets with 3 tiers of CP concentrations (210, 190, and 170 g/kg) on the performance of broiler chickens. The parameters assessed included growth performance (15-36 d posthatch), carcass traits, nutrient utilization, starch-protein digestive dynamics, apparent ileal amino acid digestibility coefficients, and the free amino acid and ammonia (NH3) concentrations in systemic plasma. Also, the feasibility of substituting soybean meal with canola meal in 190 g/kg CP diets was investigated. The dietary CP reduction from 210 to 170 g/kg significantly compromised weight gain by 12.4% (1,890 vs. 2158 g/bird) and FCR by 5.33% (1.501 vs. 1.425). The 0.5 MJ energy density reduction compromised FCR by 3.25% (1.525 vs. 1.477; P = 0.013) in birds offered 170 g/kg CP diets. Reducing dietary CP and energy densities interactively influenced (P = 0.027) apparent metabolizable energy (AME) and nitrogen corrected metabolizable energy (AMEn) (P = 0.022) such that reducing dietary CP increased these parameters but reducing dietary energy densities decreased AME and AMEn. The 150 g/kg canola meal inclusion with the elimination of soybean meal displayed some promise. Dietary CP reductions (and increased nonbound amino acid inclusions) linearly associated with increased plasma ammonia (NH3) concentrations (r = -0.607; P = 0.010) and plasma NH3 was linearly related to depressed weight gains (r = -0.565; P = 0.018). The association of dietary non-protein-bound amino acid (NPBAA) inclusions and elevated plasma NH3 concentrations have profound implications for the successful development of reduced-CP, wheat-based broiler diets.

The Contribution of Phytate-Degrading Enzymes to Chicken-Meat Production.

The contribution that exogenous phytases have made towards sustainable chicken-meat production over the past two decades has been unequivocally immense. Initially, their acceptance by the global industry was negligible, but today, exogenous phytases are routine additions to broiler diets, very often at elevated inclusion levels. The genesis of this remarkable development is based on the capacity of phytases to enhance phosphorus (P) utilization, thereby reducing P excretion. This was amplified by an expanding appreciation of the powerful anti-nutritive properties of the substrate, phytate (myo-inositol hexaphosphate; IP6), which is invariably present in all plant-sourced feedstuffs and practical broiler diets. The surprisingly broad spectra of anti-nutritive properties harbored by dietary phytate are counteracted by exogenous phytases via the hydrolysis of phytate and the positive consequences of phytate degradation. Phytases enhance the utilization of minerals, including phosphorus, sodium, and calcium, the protein digestion, and the intestinal uptakes of amino acids and glucose to varying extents. The liberation of phytate-bound phosphorus (P) by phytase is fundamental; however, the impacts of phytase on protein digestion, the intestinal uptakes of amino acids, and the apparent amino acid digestibility coefficients are intriguing and important. Numerous factors are involved, but it appears that phytases have positive impacts on the initiation of protein digestion by pepsin. This extends to promoting the intestinal uptakes of amino acids stemming from the enhanced uptakes of monomeric amino acids via Na+-dependent transporters and, arguably more importantly, from the enhanced uptakes of oligopeptides via PepT-1, which is functionally dependent on the Na+/H+ exchanger, NHE. Our comprehension of the phytate-phytase axis in poultry nutrition has expanded over the past 30 years; this has promoted the extraordinary surge in acceptance of exogenous phytases, coupled with the development of more efficacious preparations in combination with the deflating inclusion costs for exogenous phytases. The purpose of this paper is to review the progress that has been made with phytate-degrading enzymes since their introduction in 1991 and the underlying mechanisms driving their positive contribution to chicken-meat production now and into the future.

Essential amino acid recommendations for Isa Brown layers during peak and post peak production.

The present study was designed to re-evaluate the ideal amino acid ratios of total sulphur amino acids (TSAA), Thr, Val, Ile, Trp, and Arg relative to Lys during peak and post-peak production phases in laying hens by using seven independent amino acid assays in similar experimental setting. A total of 348 twenty wk old Isa Brown laying hens were allocated to individual battery cages. Each dietary treatment included 6 replicates with 2 single cages (2 birds) as one replicate. All diets were formulated based on maize, soybean meal, and canola meal to have identical crude protein (120 g/kg) concentrations and energy density (11.9 MJ/kg) but with 5 levels of dietary concentrations of tested amino acids. Hens were offered experimental diets from 27 to 33 wk of age in experiment 1 (Exp. 1) and from 42 to 48 wk of age in experiment 2 (Exp. 2). Daily egg production and weekly egg weights were recorded, and feed intakes were calculated for each experimental period to determine egg production rate, egg mass, and feed conversion ratio (FCR). Linear and quadratic broken line models were used to estimate amino acid requirements on egg production rate, egg mass and FCR. Overall, quadratic broken line models estimated higher amino acid requirements for egg mass, egg production rate and FCR than linear broken line models by 23, 25, and 20%, respectively. The predicted daily Lys intake recommendation was 720 mg/bird/day with linear broken line model and 897 mg/bird/day with quadratic broken line model and the recommended ideal amino acid ratios relative to Lys are 85 for TSAA, 69 for Thr, 83 for Val, 87 for Ile, 22 for Trp, and 82 for Arg based on linear broken line model and 87 for TSAA, 67 for Thr, 83 for Val, 86 for Ile, 22 for Trp, and 78 for Arg based on quadratic broken line model estimations.

Identifying the shortfalls of crude protein-reduced, wheat-based broiler diets.

The objective of this review is to identify the shortfalls of wheat-based, crude protein (CP)-reduced diets for broiler chickens as wheat is inferior to maize in this context but to inconsistent extents. Inherent factors in wheat may be compromising gut integrity; these include soluble non-starch polysaccharides (NSP), amylase trypsin inhibitors (ATI) and gluten. Soluble NSP in wheat induce increased gut viscosities, which can lead to compromised gut integrity, which is not entirely ameliorated by NSP-degrading feed enzymes. Wheat ATI probably compromise gut integrity and may also have the capacity to increase endogenous amino acid flows and decrease apparent starch and protein digestibilities. Gluten inclusions of 20 g/kg in a maize-soy diet depressed weight gain and feed intake and higher gluten inclusions have been shown to activate inflammatory cytokine-related genes in broiler chickens. Further research is required, perhaps particularly in relation to wheat ATI. The protein content of wheat is typically higher than maize; importantly, this results in higher inclusions of non-bound amino acids in CP-reduced broiler diets. These higher inclusions could trigger post-enteral amino acid imbalances, leading to the deamination of surplus amino acids and the generation of ammonia (NH3) which, if not adequately detoxified, results in compromised growth performance from NH3 overload. Thus, alternatives to non-bound amino acids to meet amino acid requirements in birds offered CP-reduced, wheat-based diets merit evaluation. The digestion of wheat starch is more rapid than that of maize starch which may be a disadvantage as the provision of some slowly digestible starch in broiler diets may enhance performance. Alternatively, slowly digestible starch may result in more de novo lipogenesis. Therefore, it may prove instructive to evaluate CP-reduced diets based on maize-wheat and/or sorghum-wheat blends rather than entirely wheat. This would reduce non-bound amino acid inclusions by lowering dietary CP derived from feed grains and may enhance starch digestive dynamics by retarding starch digestion rates. Also, the use of biomarkers to monitor gut integrity in broiler chickens is examined where calprotectin, ovotransferrin and possibly citrulline appear to hold promise, but their validation requires further research.

Dietary crude protein concentrations, feed grains, and whey protein interactively influence apparent digestibility coefficients of amino acids, protein, starch, and performance of broiler chickens.

The present study was designed to investigate the impacts of dietary crude protein (CP) concentrations (220 and 180 g/kg) in either maize- or wheat-based diets, without or with 25 g/kg inclusions of whey powder (WP) concentrate on performance parameters and apparent amino acid digestibility coefficients in broiler chickens. The maize and wheat used in this study had CP levels of 84 and 119 g/kg, respectively. The 2 × 2 × 2 factorial array of 8 dietary treatments was offered to a total of 336 off-sex, male Ross 308 chicks from 7 to 35 d post-hatch with 7 replicate cages (6 birds per cage) per treatment. A treatment interaction (P = 0.016) between dietary CP and feed grains was detected for weight gains, where birds offered 180 g/kg maize-based diets displayed a weight gain advantage of 6.74% (2,628 vs. 2,462 g/bird) compared to their wheat-based counterparts. An interaction (P = 0.022) between feed grains and whey protein was observed for FCR as the addition of WP to maize-based diets improved FCR by 3.45% (1.314 vs. 1.361), but compromised FCR in wheat-based diets by 2.98% (1.415 vs. 1.374). A treatment interaction (P = 0.038) between dietary CP and feed grains was recorded for relative abdominal fat-pad weights weight gains as birds offered 180 g/kg CP maize-based diets had 43.4% (11.17 vs. 7.79 g/kg) heavier fat-pads than their wheat-based counterparts. Following the reduction in dietary-CP, apparent amino acid digestibility coefficients were depressed to greater extents in wheat-based diets. However, significant interactions between CP and feed grains were found in 14 of the 16 amino acids assessed and significant interactions between CP and WP were observed for 15 amino acids. Maize was the more suitable feed grain in terms of weight gain and FCR in 180 g/kg CP diets despite causing greater fat deposition. The inclusion of WP in reduced-CP diets did not enhance bird performance. Data generated indicate concentrations of microbial amino acids in distal ileal digesta were depressing apparent amino acid digestibility coefficients, which was more evident in wheat-based diets. Higher gut viscosities in birds offered wheat-based diets may have facilitated the proliferation of microbiota along the small intestine.

Protein sources and starch-protein digestive dynamics manipulate growth performance in broiler chickens defined by an equilateral-triangle response surface design.

A total of 360 male, off-sex Ross 308 chicks were offered 10 dietary treatments from 14 to 35 d post-hatch in an equilateral-triangle response surface design feeding study in order to confirm the importance of protein and amino acid digestive dynamics in broiler chickens. The 3 apical diets were nutritionally-equivalent containing either soybean meal, non-bound amino acids or whey protein concentrate as the major source of dietary protein and amino acids. Appropriate blends of the 3 apical diets comprised the balance of 7 diets and each dietary treatment was offered to 6 replicate cages with 6 birds per cage. Growth performance, nutrient utilisation, apparent protein and starch digestibility coefficients were determined in 4 small intestinal segments. The optimal weight gain (2,085 g/bird) and feed conversion ratios (FCR, 1.397) were generated by Diet 50S50W which included a 50:50 blend of apical diets rich in whey protein concentrate and soybean meal. Broiler chickens offered Diet 50S50W also had the highest experimental and predicted jejunal digestibility (0.685 in proximal jejunum and 0.823 in distal jejunum). FCR was not correlated with apparent distal ileal digestibility coefficient (P > 0.05) of protein but was correlated with apparent protein digestibility in proximal jejunum (r = -0.369, P = 0.040) and distal jejunum (r = -0.316, P = 0.015). Surplus dietary starch was correlated with increased fat pad weight (r = 0.781, P = 0.008). The findings confirmed the relevance of protein digestion rate, reflected by jejunal digestibility, on feed conversion of broiler chickens. A balance between protein-bound and non-bound crystalline or synthetic amino acids may be required for optimal growth and protein digestion.

The Impact of Digestive Dynamics on the Bioequivalence of Amino Acids in Broiler Chickens.

The purpose of this review is to consider the distinct possibility that dietary non-bound and protein-bound amino acids are not bioequivalent in broiler chickens. Usually, with conservative inclusions of a limited number of non-bound (synthetic, crystalline, feed-grade) amino acids in standard broiler diets, bioequivalency would not be an issue. However, reduced-crude protein (CP) broiler diets demand substantial inclusions of an extended range of non-bound amino acids to meet amino acid requirements. A standard diet may contain 5.0 g/kg non-bound amino acids, but a reduced-CP diet may contain up to 50 g/kg and this relative abundance skews the balance of non-bound to protein-bound amino acids and substantial proportions of certain amino acids are present in diets as non-bound entities. Importantly, tangible reductions in dietary CP, for example from 210 to 160 g/kg, usually both compromise broiler growth performance and increase fat deposition. Compromised growth performance is more evident in wheat- than maize-based diets but, paradoxically, fat deposition is more apparent in maize-based diets. The inability of birds to accommodate tangible dietary CP reductions appears to stem partially from the lack of bioequivalency between non-bound and protein-bound amino acids because of the differentials in intestinal uptake rates. Also, reduced-CP broiler diets generate perturbations in apparent amino acid digestibility coefficients which compound the fact that intestinal uptakes of non-bound acid acids are more rapid, and occur more anteriorly in the small intestine, than protein-bound amino acids. The likelihood is that greater proportions of non-bound amino acids transit the enterocytes of the gut mucosa without entering anabolic and/or catabolic pathways to gain entry to the portal circulation. This culminates in post-enteral amino acid imbalances and postprandial oxidation of surplus amino acids which involves deamination of amino acids and elevations in plasma ammonia (NH3) concentrations, but NH3 is inherently toxic and demands detoxification. Excessive deamination coupled with inadequate detoxification could result in 'ammonia overload' which would be expected to compromise growth performance. Thus, the hypothesis is that non-bound and protein-bound amino acids are not bioequivalent; moreover, it may be argued that this distinction is being overlooked and is thwarting the development and acceptance of reduced-CP broiler diets.

A multivariate Box-Behnken assessment of elevated branched-chain amino acid concentrations in reduced crude protein diets offered to male broiler chickens.

In a Box-Behnken assessment of elevated branched-chain amino acids (BCAA), 13 nutritionally equivalent maize-based diets were offered to a total of 390 off-sex male Ross 308 broiler chickens from 7 to 28 days post-hatch. The BCAA concentrations investigated in reduced-crude protein diets were 12.5, 15.5, 18.3 g/kg leucine (125, 155, 183); 8.9, 10.2, 12.5 g/kg valine (89, 102, 125) and 7.2, 8.9, 10.8 g/kg isoleucine (72, 89, 109), where their relativity to 11.0 g/kg digestible lysine are shown in parentheses. Determined parameters included growth performance, relative abdominal fat-pad weights, nutrient utilisation, apparent digestibility coefficients, disappearance rates of 16 amino acids and free amino acid systemic plasma concentrations. Increasing dietary leucine linearly depressed weight gain and quadratically influenced FCR where the estimated minimum FCR of 1.418 was with 14.99 g/kg leucine. Polynomial regression analysis and surface response curves of determined parameters were generated for significant (P < 0.05) BCAA variables, based on lack of fit (P > 0.005). Quadratic and cross-product responses were observed for weight gain, FCR, AME, AMEn, N retention and apparent digestibility of 13 amino acids. Relative fat-pad weights declined linearly with increasing isoleucine and valine. The lowest N retention was estimated at a combination of 15.25 and 10.50 g/kg leucine and valine respectively whilst the highest mean digestibility coefficient (0.793) of amino acids was estimated at a combination of 15.74 and 10.47 g/kg of leucine and valine respectively. The remaining parameter minima or maxima responses were not able to be determined since they were outside the extreme BCAA treatment levels. Increasing dietary BCAA significantly increased apparent ileal digestibilities and disappearance rates of BCAA. Systemic plasma concentrations of valine increased (P < 0.001) with increasing dietary valine but leucine was not influenced (P > 0.25). Systemic plasma concentration of isoleucine was maximised (P < 0.001) only when accompanied by elevated dietary leucine. Also, dietary treatments influenced (P < 0.05) apparent disappearance rates of all the essential amino acids analysed, with the exception of methionine. Whilst overall growth performance was not disadvantaged (P > 0.10) by elevated BCAA levels, compared with 2019 Ross 308 performance objectives, polynomial regression analysis suggested both interaction and antagonism between BCAA.

Graded methionine dietary inclusions influence growth performance and apparent ileal amino acid digestibility coefficients and disappearance rates in broiler chickens.

Graded quantities of 1.38, 2.76 and 4.14 g/kg L-methionine were included in a control diet formulated to contain 3.07 g/kg digestible methionine. Each of the 4 dietary treatments was offered to 6 replicate cages (initially 8 birds per cage) from 1 to 21 d post-hatch. The parameters assessed included growth performance, nutrient utilisation (apparent metabolisable energy [AME], AME:GE ratios, N retention, N-corrected apparent metabolisable energy [AMEn]), apparent digestibility coefficients and disappearance rates of amino acids in the distal ileum. They also included free amino concentrations in systemic plasma (brachial vein) at 20 d post-hatch and in hepatic tissue at 14 and 21 d post-hatch. Graded L-methionine inclusions quadratically influenced weight gain (r = 0.688; P = 0.001) and FCR (r = 0.780; P < 0.001). It may be deduced from the quadratic regressions that 3.43 g/kg L-methionine supported maximum weight gain of 1,036 g/kg and 3.50 g/kg L-methionine minimum FCR of 1.193, from 1 to 21 d post-hatch. The control diet contained specified levels of 3.07 g/kg digestible methionine and 13.0 g/kg digestible lysine. Thus, an inclusion of 3.465 g/kg L-methionine corresponded to a total of 6.535 g/kg methionine or a methionine-to-lysine ratio of 50.3, which is higher than standard recommendations. The implications of this and other outcomes of the present study are reported and discussed.

Evaluation of dietary crude protein concentrations, fishmeal, and sorghum inclusions in broiler chickens offered wheat-based diet via Box-Behnken response surface design.

The objective of this study was to investigate the impacts of dietary crude protein (CP), fishmeal and sorghum on nutrient utilisation, digestibility coefficients and disappearance rates of starch and protein, amino acid concentrations in systemic plasma and their relevance to growth performance of broiler chickens using the Box-Behnken response surface design. The design consisted of three factors at three levels including dietary CP (190, 210, 230 g/kg), fishmeal (0, 50, 100 g/kg), and sorghum (0, 150, 300 g/kg). A total of 390 male, off-sex Ross 308 chicks were offered experimental diets from 14 to 35 days post-hatch. Growth performance, nutrient utilisation, starch and protein digestibilities and plasma free amino acids were determined. Dietary CP had a negative linear impact on weight gain where the transition from 230 to 190 g/kg CP increased weight gain by 9.43% (1835 versus 2008 g/bird, P = 0.006). Moreover, dietary CP linearly depressed feed intake (r = -0.486. P < 0.001). Fishmeal inclusions had negative linear impacts on weight gain (r = -0.751, P < 0.001) and feed intake (r = -0.495, P < 0.001). There was an interaction between dietary CP and fishmeal for FCR. However, growth performance was not influenced by dietary inclusions of sorghum. Total plasma amino acid concentrations were negatively related to weight gain (r = -0.519, P < 0.0001). The dietary transition from 0 to 100 g/kg fishmeal increased total amino acid concentrations in systemic plasma by 35% (771 versus 1037 µg/mL, P < 0.001). It may be deduced that optimal weight gain (2157 g/bird), optimal feed intake (3330 g/bird) and minimal FCR (1.544) were found in birds offered 190 g/kg CP diets without fishmeal inclusion, irrespective of sorghum inclusions. Both fishmeal and sorghum inclusions did not alter protein and starch digestion rate in broiler chickens; however, moderate reductions in dietary CP could advantage broiler growth performance.

Implications of excreta uric acid concentrations in broilers offered reduced-crude protein diets and dietary glycine requirements for uric acid synthesis.

In a previous experiment, male Ross 308 broiler chickens were offered dietary treatments with 3 levels of crude protein (222, 193, 165 g/kg) and 3 feed grains (ground maize, ground wheat, whole wheat) from 7 to 35 d post-hatch. Maize-based diets supported superior growth performance in comparison to wheat-based diets. Uric acid concentrations in excreta were retrospectively determined and related to total nitrogen (N) excreta concentrations. Uric acid concentrations ranged from 28.5 to 69.4 mg/g and proportions of uric acid-N to total excreta-N ranged from 27.4% to 42.6% in broiler chickens offered the 3 × 3 factorial array of dietary treatments. Proportions of uric acid-N to total N in excreta in birds offered the 165 g/kg CP, maize-based diet were significantly lower by 10.6 percentage units (27.4% versus 38.0%; P = 0.00057) than their wheat-based counterparts. Total excreta analysed had been collected from 35 to 37 d post-hatch when feed intakes and excreta outputs were monitored. There were linear relationships between proportions of uric acid-N to total N in excreta in birds offered the three 165 g/kg CP diets with weight gain (r = -0.587; P = 0.010), feed intake (r = -0.526; P = 0.025) and feed conversion ratios (r = 0.635; P = 0.005). The possibility that increasing uric acid-N proportions in excreta is indicative of excessive ammonia accumulations compromising growth performance is discussed. The mean proportion of dietary glycine involved in uric acid excretion was 49.2% across all dietary treatments but ranged from 25.0% to 80.9%. Thus, the appropriate amount of dietary glycine is variable and largely dependent on the volume of uric acid synthesised and excreted.

The Dynamic Conversion of Dietary Protein and Amino Acids into Chicken-Meat Protein.

This review considers the conversion of dietary protein and amino acids into chicken-meat protein and seeks to identify strategies whereby this transition may be enhanced. Viable alternatives to soybean meal would be advantageous but the increasing availability of non-bound amino acids is providing the opportunity to develop reduced-crude protein (CP) diets, to promote the sustainability of the chicken-meat industry and is the focus of this review. Digestion of protein and intestinal uptakes of amino acids is critical to broiler growth performance. However, the transition of amino acids across enterocytes of the gut mucosa is complicated by their entry into either anabolic or catabolic pathways, which reduces their post-enteral availability. Both amino acids and glucose are catabolised in enterocytes to meet the energy needs of the gut. Therefore, starch and protein digestive dynamics and the possible manipulation of this 'catabolic ratio' assume importance. Finally, net deposition of protein in skeletal muscle is governed by the synchronised availability of amino acids and glucose at sites of protein deposition. There is a real need for more fundamental and applied research targeting areas where our knowledge is lacking relative to other animal species to enhance the conversion of dietary protein and amino acids into chicken-meat protein.

Progress towards reduced-crude protein diets for broiler chickens and sustainable chicken-meat production.

The prime purpose of this review is to explore the pathways whereby progress towards reduced-crude protein (CP) diets and sustainable chicken-meat production may be best achieved. Reduced-CP broiler diets have the potential to attenuate environmental pollution from nitrogen and ammonia emissions; moreover, they have the capacity to diminish the global chicken-meat industry's dependence on soybean meal to tangible extents. The variable impacts of reduced-CP broiler diets on apparent amino acid digestibility coefficients are addressed. The more accurate identification of amino acid requirements for broiler chickens offered reduced-CP diets is essential as this would diminish amino acid imbalances and the deamination of surplus amino acids. Deamination of amino acids increases the synthesis and excretion of uric acid for which there is a requirement for glycine, this emphasises the value of so-called "non-essential" amino acids. Starch digestive dynamics and their possible impact of glucose on pancreatic secretions of insulin are discussed, although the functions of insulin in avian species require clarification. Maize is probably a superior feed grain to wheat as the basis of reduced-CP diets; if so, the identification of the underlying reasons for this difference should be instructive. Moderating increases in starch concentrations and condensing dietary starch:protein ratios in reduced-CP diets may prove to be advantageous as expanding ratios appear to be aligned to inferior broiler performance. Threonine is specifically examined because elevated free threonine plasma concentrations in birds offered reduced-CP diets may be indicative of compromised performance. If progress in these directions can be realised, then the prospects of reduced-CP diets contributing to sustainable chicken-meat production are promising.

Amino acid requirements for laying hens: a comprehensive review.

The main aim of this review is to consolidate the relevant published data examining amino acid requirements of layer hens and to reach a new set of recommendation based on these data. There are inconsistences in lysine, sulphur-containing amino acids, threonine, tryptophan, branched-chain amino acids, and arginine recommendations in data that have surfaced since 1994. This review finds that breed, age, basal diet composition, and assessment method have contributed toward inconsistencies in amino acid recommendations. Presently, the development of reduced-protein diets for layer hens is receiving increasing attention because of the demand for sustainable production. This involves quite radical changes in diet composition with inclusions of nonbound, essential and nonessential amino acids. Increasing inclusions of nonbound amino acids into layer diets modifies protein digestive dynamics, and it may influence amino acid requirements in layer hens. This review considers present amino acid recommendations for layer hens and proposes refinements that may better serve the needs of the layer industry in the future.

Protein digestive dynamics of meat and bone meals in broiler chickens.

This study determined the variations in protein digestibilities and digestion rates in broiler chickens offered diets containing 7 different meat and bone meals (MBM). A total of 252 male Ross 308 broiler chickens were offered 7 atypical diets largely based on maize and MBM from 24 to 28 d post-hatch. Each experimental diet was offered to 6 replicates with 6 birds per replicate cage. Excreta were collected in their entirety from 25 to 27 d post-hatch and on 28 d post-hatch. Digesta samples were collected from the proximal jejunum, distal jejunum, proximal ileum and distal ileum. Apparent digestibilities of protein were determined in each segment and apparent digestibilities of amino acids were measured in the distal ileum. There were significant differences in apparent protein digestibility coefficients in the proximal jejunum (P = 0.006), where broiler chickens offered the high ash beef meal (diet 7) generated the lowest protein digestibility in the proximal jejunum (0.318). Similarly, there were significant differences in apparent digestibility coefficients in the distal jejunum (P < 0.001) and distal ileum (P < 0.001) but not in the proximal ileum. More pronounced differences were found in the disappearance rate of protein and there were significant differences in all 4 segments of the small intestine (P < 0.001). Broiler chickens offered the high ash beef meal had the lowest protein disappearance rate (P < 0.001). No difference was observed in the predicted protein digestion rate (P = 0.486) but chickens offered the high ash beef meal had the lowest potential digestible protein (0.662, P = 0.034) whereas the highest potential digestible protein (0.739) was detected in diet 5 (containing a beef meal). This study contributed to the establishment of a preliminary database to include digestion rates of starch and protein into practical diet formulation.

Effects of Adding Phytase from Aspergillus niger to a Low Phosphorus Diet on Growth Performance, Tibia Characteristics, Phosphorus Excretion, and Meat Quality of Broilers 35 days after hatching.

This study was designed to determine the effect of phytase extracted from Aspergillus niger (Natuphos® E) on growth performance, bone mineralization, phosphorous excretion, and meat quality parameters in broilers fed available phosphorous (aP)-deficient diet. In total, 810 one-day-old Indian River broilers were randomly allotted into one of three dietary treatments, with six replicates per treatment. The three dietary treatments were 1) control group (CON: basal diet with sufficient aP), 2) low phytase (LPY: available phosphorus-deficient diet supplemented with 0.01% phytase), and 3) high phytase (HPY: available phosphorus-deficient diet supplemented with 0.02% phytase). Average daily gain and, feed intake, and feed conversion ratio were measured for 35 days. Excreta were collected from each pen on day 35. One broiler from each cage was euthanized to collect visceral organs and tibia samples. Broiler chickens fed LPY and HPY showed improved (P<0.05) growth performance compared to broilers fed CON on day 35. The tibia length of HPY-fed broilers was more than those of broilers fed other diets on day 35 (P<0.05). However, tibia calcium and phosphorous contents in LPY-fed broilers was higher (P<0.05) than in CON and HPY-fed broilers. Tibia length and calcium and phosphorous content showed a positive correlation (P<0.05) with the weight gain of broilers on day 35. Phosphorous level in the excreta of LPY- and HPY-fed broilers was lesser than those of CON broilers on day 35 (P<0.05). Furthermore, HPY-fed broilers showed lower (P<0.05) phosphorous content in the excreta than LPY-fed broilers. LPY- and HPY-fed broilers showed higher (P<0.05) liver weight than the CON broilers. In conclusion, broilers fed aP-deficient diet supplemented with phytase from Aspergillus excreted less phosphorus, which enhanced growth performance and tibia development from time of hatching to day 35 post-hatching.

Broilers fed a low protein diet supplemented with synthetic amino acids maintained growth performance and retained intestinal integrity while reducing nitrogen excretion when raised under poor sanitary conditions.

The present study investigated the effects of supplementing a low protein (LP) diet supplemented with key essential amino acids (AA) to broilers on growth performance, intestinal tract function, blood metabolites, and nitrogen excretion when the animals were maintained under various sanitary conditions for 35 D after hatching. Three hundred eighty-four one-day-old male broilers (Ross 308) were randomly allotted to groups that received one of 6 dietary treatments in a 2 × 3 factorial arrangement (i.e., 2 environmental conditions and 3 dietary treatments) to give 8 replicates per treatment. Broilers were challenged with 2 environmental conditions (sanitary vs. poor sanitary). The dietary treatments were (1) high protein (HP) diet, (2) LP diet, and (3) LP diet with synthetic key essential AA (LPA): the LP diet was supplemented with synthetic AA up to the required levels for broilers. On day 14, birds consumed the LP diet impaired growth performance compared with those fed the HP diet, while the average daily weight gain-to-feed conversion ratio of birds fed the LPA diet improved to the level of birds fed the HP diet under poor sanitary conditions (P < 0.05). Broilers raised under poor sanitary conditions and fed the LP diet displayed higher (P < 0.05) zonula occludens (ZO-1) expression on day 14 than broilers fed either the HP or LPA diet. Under sanitary conditions, birds fed HP and LPA diets showed higher villus height and crypt depth compared with those of broilers fed the LP diet on day 35. Moreover, broilers raised in the poor sanitary environment had higher (P < 0.05) serum endotoxins than those raised in the sanitary environment. Broilers fed the LPA diet showed reduced (P < 0.05) nitrogen excretion on days 14 and 35 compared with those fed the LP and HP diets independent of the environment. In conclusion, the LPA diet did not impair growth performance under poor sanitary conditions for 14 D after hatch while resulting in lower nitrogen excretion in any environment conditions throughout the experiment.