Changes in the Lipid Metabolism of the Longissimus thoracis Muscle in Bulls When Using Different Feeding Strategies during the Growing and Finishing Phases.

The objective was to evaluate the supplementation strategy's effect on beef cattle during the growing phase and two systems during the finishing phase. One hundred and twenty young bulls were randomly divided in a 2 × 2 factorial design to receive either mineral (ad libitum) or protein + energy (3 g/kg body weight (BW)/day) during the growing phase and pasture plus concentrate supplementation (20 g/kg BW/day) or feedlot (25:75% corn silage:concentrate) during the finishing phase. Feedlot-fed bulls had meat (Longissimus thoracis-LT) with a higher content of lipids and saturated and monounsaturated fatty acids and a greater upregulation of stearoyl-CoA desaturase and sterol regulatory element-binding protein-1c than animals that fed on pasture (p < 0.05). On the other hand, pasture-fed bulls had meat with a higher content of α-linoleic acid, linolenic acid, and n6 and a greater n6:n3 ratio compared to the feedlot-fed group (p < 0.05). In addition, meat from pasture-fed bulls during the finishing phase had 17.6% more isocitrate dehydrogenase enzyme concentration than the feedlot group (p = 0.02). Mineral-fed and pasture-finished bulls showed down-regulation of peroxisome proliferator-activated receptor gamma (p < 0.05), while the bulls fed protein + energy and finished in the feedlot had higher carnitine palmitoyltransferase 2 expression (p ≤ 0.013). In conclusion, mineral or protein + energy supplementation in the growing does not affect the fatty acid composition of intramuscular fat of LT muscle. In the finishing phase, feeding bulls in the feedlot upregulates the lipogenic genes and consequently improves the intramuscular fat content in the meat.

Effect of Cow-Calf Supplementation on Gene Expression, Processes, and Pathways Related to Adipogenesis and Lipogenesis in Longissimus thoracis Muscle of F1 Angus × Nellore Cattle at Weaning.

The aim of this study was to identify differentially expressed genes, biological processes, and metabolic pathways related to adipogenesis and lipogenesis in calves receiving different diets during the cow-calf phase. Forty-eight uncastrated F1 Angus × Nellore males were randomly assigned to two treatments from thirty days of age to weaning: no creep feeding (G1) or creep feeding (G2). The creep feed offered contained ground corn (44.8%), soybean meal (40.4%), and mineral core (14.8%), with 22% crude protein and 65% total digestible nutrients in dry matter. After weaning, the animals were feedlot finished for 180 days and fed a single diet containing 12.6% forage and 87.4% corn-based concentrate. Longissimus thoracis muscle samples were collected by biopsy at weaning for transcriptome analysis and at slaughter for the measurement of intramuscular fat content (IMF) and marbling score (MS). Animals of G2 had 17.2% and 14.0% higher IMF and MS, respectively (p < 0.05). We identified 947 differentially expressed genes (log2 fold change 0.5, FDR 5%); of these, 504 were upregulated and 443 were downregulated in G2. Part of the genes upregulated in G2 were related to PPAR signaling (PPARA, SLC27A1, FABP3, and DBI), unsaturated fatty acid synthesis (FADS1, FADS2, SCD, and SCD5), and fatty acid metabolism (FASN, FADS1, FADS2, SCD, and SCD5). Regarding biological processes, the genes upregulated in G2 were related to cholesterol biosynthesis (EBP, CYP51A1, DHCR24, and LSS), unsaturated fatty acid biosynthesis (FADS2, SCD, SCD5, and FADS1), and insulin sensitivity (INSIG1 and LPIN2). Cow-calf supplementation G2 positively affected energy metabolism and lipid biosynthesis, and thus favored the deposition of marbling fat during the postweaning period, which was shown here in an unprecedented way, by analyzing the transcriptome, genes, pathways, and enriched processes due to the use of creep feeding.

Assessment of corn wet distillers grains fed to crossbred bulls on feeding behavior, rumen morphology, liver abscesses and blood parameters.

Corn ethanol production has been growing in Brazil in the last ten years, generating by-products to feedlot diets. This study evaluates the effects of the inclusion of low-fat corn wet distillers grains (LF-WDG) on feeding behavior, ruminal health, liver abscesses and blood parameters of F1 Angus-Nellore bulls feedlot finished. Our hypothesis is that evaluation of data from feeding behavior, rumen and liver health would help to explain animal performance. In this trail, one-hundred animals were fed for 129 days with diets containing amounts of 0 (control), 15, 30 and 45% of LF-WDG replacing corn grain and soybean meal. Evaluations of fluctuation of dry matter intake (DMI) were carried out. Additionally, feeding behavior data were assessed by monitoring (24-h period) the feeding, rumination, time spent eating (TSE), and time expended on other activities (resting and number of meals per day). Blood variables such as pH, bicarbonate, total CO2 content, and base excess in extracellular fluid (Beecf) were determined. After slaughter, rumen epithelium was classified according to the incidence of lesions (rumenitis) and abnormalities (papillae clumped), and samples were collected for morphology and histology evaluations. Moreover, livers were scored for severity of abscesses as follow: as unabscessed (0), one or two small abscesses (A-), two to four small active abscesses (A) or one or more large, active abscesses (A+). The DMI (kg/day) differed (P = 0.03) among treatments and there is a tendency of 15 and 30 LF-WDG (% DM) had lower %DMI fluctuation compared to 0 or 45%. The TSE increased linearly (P < 0.01) as the amounts of inclusion of LF-WDG increased. Moreover, neutral detergent fiber (NDF) intake, NDF consumption rate and NDF rumination efficiency increased linearly (P < 0.01) in response to LF-WDG feeding. The incidence of rumenitis tended (P = 0.08) to be greater at 45% LF-WDG, while keratin thickness decreased linearly in bulls fed LF-WDG (P < 0.01). The severity of liver abscesses (score A+) increased linearly (P = 0.02). Regarding blood parameters, only Beecf decreased linearly (P < 0.01) in response to LF-WDG feeding. Therefore, the hypothesis of the current study was confirmed. We previous reported that F1 Angus-Nellore bulls fed LF-WDG show greater weight gain (1.94 ± 0.09 kg/day) and final body weight (620 ± 18.8 kg) when compare to control (1.8 ± 0.09 kg/day and 602 ± 18.8 kg, respectively). Here, we conclude that inclusion of 15 to 30% LF-WDG in feedlot diets improved feeding behavior without impairing ruminal health and blood parameters, driving performance and weigh gain of crossbred bulls. However, bulls fed 45% LF-WDG had greater severity of liver abscesses.

Comparison of Dental Carcass Maturity in Non-Castrated Male F1 Angus-Nellore Cattle Finished in Feedlot.

Dental classification of carcasses is used as a parameter of cattle maturity at slaughter, and it can influence carcass and meat quality traits. Brazilian beef-packing companies use the number of permanent incisor (PI) teeth as a parameter for bonus and certification of carcasses with superior quality. However, when non-castrated male such as F1 Angus-Nellore (Bos taurus×Bos indicus) are slaughtered, only animals without PI teeth are subsidized by the breed association. We evaluated these animals finished in feedlot for 180 days with zero versus two PI teeth on the carcass and meat quality traits. At the time of slaughter, 88 carcasses were selected, forming two treatments according to dental carcass maturity (0 versus 2 PI teeth; 44 animals per category). It was demonstrated that the number of PI teeth (0 versus 2 PI) did not influence (p>0.05) carcass (weights, yield, cooling loss, ribeye area and the backfat thickness) and meat quality traits (Longissimus thoracis chemical composition, color, cooking losses, shear force and pH). Thus, dental carcass maturity (zero versus two PI teeth) does not influence non-castrated male F1 Angus-Nellore finished in feedlot for 180 days. This is the first study to demonstrate that carcasses of non-castrated male F1 Angus-Nellore with two PI teeth should be subsidized in a similar way to those with zero PI teeth. Moreover, Brazilian beef-packing companies could produce heavier and leaner carcasses of acceptable quality though the use of crossbred cattle such as non-castrated F1 Angus Nellore.

Carcass characteristics of zebu steers receiving different oleaginous grains / Características de carcaça de novilhos zebuínos recebendo diferentes grãos de oleaginosas

This research aimed to evaluate carcass traits of Zebu steers fed different oleaginous grains. Thirty one 23 months old zebu steers with 365 + 37.5 kg of live weight were used. The diets consisted of corn silage and four different concentrates; no additional lipids and three different ground oilseeds sources: soybean, cottonseed and linseed. The diets had concentrate: roughage ratio of 60:40 and were offered ad libitum. The experimental period was of 84 days, preceded by a 28 day adaptation period. The experiment was carried out in a randomized block design and the means were compared by Scott-Knott test at 5% level of probability. The CS inclusion of into the diet decreased (p < 0.05) carcass yield of the animals. The hindquarter was heavier (p < 0.05) with the addition of SB to the diet. The spare ribs had the highest performance (p < 0.05) and outside flat was heavier (p < 0.05) when the animals were submitted to CS and FS diets. The CS addition to the diet improved carcass characteristics.

Este trabalho foi realizado com o objetivo de avaliar as características da carcaça em novilhos Zebuínos alimentados com inclusão de fontes de lipídeos moídas. Utilizaram-se 31 novilhos Zebuínos com idade média inicial de 23 meses e peso vivo inicial de 365 + 37,5 kg. As dietas consistiram em silagem de milho e quatro concentrados, sendo um sem lipídeo adicional (SLA) e três com a inclusão de diferentes grãos de oleaginosas moídas: grão de soja (GS), caroço de algodão (CA) e semente de linhaça (SL). As rações apresentaram relação concentrado: volumoso 60:40 e foram fornecidas ad libitum. O período experimental teve duração de 84 dias sendo precedido de um período de adaptação de 28 dias. O delineamento utilizado foi em blocos casualizados, sendo as médias dos tratamentos comparadas pelo teste Scott & Knott no nível de 5% de probabilidade. Com a inclusão de CA observou-se diminuição (p < 0,05) do rendimento de carcaça dos animais. O traseiro especial foi mais pesado (p < 0,05) com a inclusão GS na dieta. O maior rendimento (p < 0,05) da ponta de agulha e maior peso (p < 0,05) de coxão duro foram verificados com a inclusão de CA e SL na dieta. A inclusão de GS na dieta melhora as características de carcaça.

Mesquite pod meal in elephant grass silages - doi: 10.4025/actascianimsci.v35i3.12506

This study evaluated the chemical composition and in vitro dry matter digestibility (IVDMD) of elephant grass silages at different growth stages (70, 90 and 110 days), with the addition (0, 5, 10 and 15%, on a fresh matter basis) of mesquite pod meal. A completely randomized design (CRD) was used in a factorial arrangement with four replications. PVC pipes 100 mm in diameter were used as experimental silos. After 30 days of ensilage, samples were taken from the open silos to determine chemical composition and IVDMD. The inclusion of mesquite pod meal (MPM) increased (p 0.01) was detected between MPM concentrations and elephant grass cutting age for DM, CP and NDF contents in the silages. A decrease (p < 0.01) was observed in NDF, ADF and MM concentrations with the inclusion of MPM. There was a linear increase (p < 0.01) in IVDMD with increasing levels of MPM; and IVDMD decreased (p < 0.01) with increasing cutting age of the elephant grass. The inclusion of mesquite pod meal contributed to improve the chemical composition and digestibility of silages.

Objetivou-se avaliar a composição químico-bromatológica e a digestibilidade “in vitro” da matéria seca (DIVMS) de silagens de capim-elefante em diferentes tempos (70; 90 e 110 dias) de crescimento do capim, com inclusão (0, 5, 10 e 15%) do farelo da vagem de algaroba. Foi utilizado um delineamento inteiramente casualizado (DIC), arranjado em um esquema fatorial, com quatro repetições. Como silos experimentais foram utilizados tubos do tipo PVC com 100 mm de diâmetro. Passados 30 dias da ensilagem, os silos foram abertos e retiradas amostras para determinação da composição químico-bromatológica e DIVMS. A inclusão do farelo da vagem de algaroba (FVA) elevou os teores de MS e PB. Não foi observada interação (p > 0,01) concentração do FVA × idade de corte do capim-elefante nos teores de MS, PB e FDN das silagens. Foi observada diminuição (p < 0,01) nos teores de FDN, FDA e MM com a inclusão do FVA. Houve aumento linear (p < 0,01) na DIVMS com o aumento na concentração do FVA e diminuição (p < 0,01) na DIVMS com o aumento da idade de corte do capim-elefante. A inclusão do farelo da vagem de algaroba contribui para melhorar a composição química e a digestibilidade das silagens.

Mesquite pod meal in elephant grass silages - doi: 10.4025/actascianimsci.v35i3.12506

This study evaluated the chemical composition and in vitro dry matter digestibility (IVDMD) of elephant grass silages at different growth stages (70, 90 and 110 days), with the addition (0, 5, 10 and 15%, on a fresh matter basis) of mesquite pod meal. A completely randomized design (CRD) was used in a factorial arrangement with four replications. PVC pipes 100 mm in diameter were used as experimental silos. After 30 days of ensilage, samples were taken from the open silos to determine chemical composition and IVDMD. The inclusion of mesquite pod meal (MPM) increased (p < 0.01) the concentrations of DM and CP. No interaction (p > 0.01) was detected between MPM concentrations and elephant grass cutting age for DM, CP and NDF contents in the silages. A decrease (p < 0.01) was observed in NDF, ADF and MM concentrations with the inclusion of MPM. There was a linear increase (p < 0.01) in IVDMD with increasing levels of MPM; and IVDMD decreased (p < 0.01) with increasing cutting age of the elephant grass. The inclusion of mesquite pod meal contributed to improve the chemical composition and digestibility of silages.(AU) 

Objetivou-se avaliar a composição químico-bromatológica e a digestibilidade “in vitro” da matéria seca (DIVMS) de silagens de capim-elefante em diferentes tempos (70; 90 e 110 dias) de crescimento do capim, com inclusão (0, 5, 10 e 15%) do farelo da vagem de algaroba. Foi utilizado um delineamento inteiramente casualizado (DIC), arranjado em um esquema fatorial, com quatro repetições. Como silos experimentais foram utilizados tubos do tipo PVC com 100 mm de diâmetro. Passados 30 dias da ensilagem, os silos foram abertos e retiradas amostras para determinação da composição químico-bromatológica e DIVMS. A inclusão do farelo da vagem de algaroba (FVA) elevou os teores de MS e PB. Não foi observada interação (p > 0,01) concentração do FVA × idade de corte do capim-elefante nos teores de MS, PB e FDN das silagens. Foi observada diminuição (p < 0,01) nos teores de FDN, FDA e MM com a inclusão do FVA. Houve aumento linear (p < 0,01) na DIVMS com o aumento na concentração do FVA e diminuição (p < 0,01) na DIVMS com o aumento da idade de corte do capim-elefante. A inclusão do farelo da vagem de algaroba contribui para melhorar a composição química e a digestibilidade das silagens.(AU)

Carcass characteristics of zebu steers receiving different oleaginous grains / Características de carcaça de novilhos zebuínos recebendo diferentes grãos de oleaginosas

This research aimed to evaluate carcass traits of Zebu steers fed different oleaginous grains. Thirty one 23 months old zebu steers with 365 + 37.5 kg of live weight were used. The diets consisted of corn silage and four different concentrates; no additional lipids and three different ground oilseeds sources: soybean, cottonseed and linseed. The diets had concentrate: roughage ratio of 60:40 and were offered ad libitum. The experimental period was of 84 days, preceded by a 28 day adaptation period. The experiment was carried out in a randomized block design and the means were compared by Scott-Knott test at 5% level of probability. The CS inclusion of into the diet decreased (p < 0.05) carcass yield of the animals. The hindquarter was heavier (p < 0.05) with the addition of SB to the diet. The spare ribs had the highest performance (p < 0.05) and outside flat was heavier (p < 0.05) when the animals were submitted to CS and FS diets. The CS addition to the diet improved carcass characteristics.(AU)

Este trabalho foi realizado com o objetivo de avaliar as características da carcaça em novilhos Zebuínos alimentados com inclusão de fontes de lipídeos moídas. Utilizaram-se 31 novilhos Zebuínos com idade média inicial de 23 meses e peso vivo inicial de 365 + 37,5 kg. As dietas consistiram em silagem de milho e quatro concentrados, sendo um sem lipídeo adicional (SLA) e três com a inclusão de diferentes grãos de oleaginosas moídas: grão de soja (GS), caroço de algodão (CA) e semente de linhaça (SL). As rações apresentaram relação concentrado: volumoso 60:40 e foram fornecidas ad libitum. O período experimental teve duração de 84 dias sendo precedido de um período de adaptação de 28 dias. O delineamento utilizado foi em blocos casualizados, sendo as médias dos tratamentos comparadas pelo teste Scott & Knott no nível de 5% de probabilidade. Com a inclusão de CA observou-se diminuição (p < 0,05) do rendimento de carcaça dos animais. O traseiro especial foi mais pesado (p < 0,05) com a inclusão GS na dieta. O maior rendimento (p < 0,05) da ponta de agulha e maior peso (p < 0,05) de coxão duro foram verificados com a inclusão de CA e SL na dieta. A inclusão de GS na dieta melhora as características de carcaça.(AU)

Viabilidade econômica da terminação de novilhos Nelore e Red Norte em confinamento na região de Lavras-MG / Economical viability of finishing Nellore and Red Norte bulls in feedlot, in Lavras-MG region

Conduziu-se este trabalho, com o objetivo de verificar a viabilidade econômica da terminação em confinamento de novilhos Nelore e Red Norte na região de Lavras, MG. Foram utilizados 41 novilhos, não castrados, de dois grupos genéticos, sendo: 19 Nelore e 22 Red Norte, com pesos vivos iniciais médios de 315 ±31 kg e 321 ±30 kg, respectivamente. O período experimental foi de 112 dias, divididos em quatro períodos de 28 dias. A viabilidade econômica foi analisada utilizando o modelo de custo, considerando os custos operacionais, alternativos e de depreciação. Todos os indicadores econômicos gerados apresentaram resultados negativos. A atividade apresentou prejuízo de R$ 6,88 e 19,74/arroba para os animais Red Norte e Nelore, respectivamente. Os resultados negativos obtidos nessas condições ocorreram porque o custo operacional efetivo foi maior que o preço de venda da arroba. Em um cenário com a aquisição de animais a atividade de confinamento apresentou resíduo próximo de zero para o grupo Red Norte. A prática do confinamento não apresentou viabilidade econômica para a região de Lavras, MG, no ano de 2007.

The objective was to study the economic viability of feedlot in Lavras region, state of Minas Gerais. Forty one bulls of two genetic groups were used, being: 19 Nellore and 22 Red Norte, with initial average age of 20 months and initial average live weight of 315 ±31 kg and 321 ±30 kg, respectively. The experimental period was 112 days. The model of cost was used to evaluate the economic viability. All economic indexes presented negative results. The activity presented negative profit of R$ 6.88 and R$ 19.74/arroba for the animals Red Norte and Nellore, respectively. The negative results obtained in these conditions took place because the operational effective cost was bigger than the sale price of the arroba. The feedlot didn't present economical viability for the Lavras region, in the year of 2007. The feed efficiency has straight influence on the economical viability of feedlot.