Management of Colic in the Field.

Colic is one of the most frequent emergencies necessitating veterinary attention. Referral is not an option in many cases; therefore, the ability to diagnose and treat colic in an ambulatory setting is paramount. Portable imaging and point-of-care testing has improved the ability to identify lesions and assess the patient's status. In cases when field management is the only option, practitioners should be aware of the various treatment options available.

Comparison of the microbiome, metabolome, and lipidome of obese and non-obese horses.

Metabolic diseases such as obesity and type 2 diabetes in humans have been linked to alterations in the gastrointestinal microbiota and metabolome. Knowledge of these associations has improved our understanding of the pathophysiology of these diseases and guided development of diagnostic biomarkers and therapeutic interventions. The cellular and molecular pathophysiology of equine metabolic syndrome (EMS) and obesity in horses, however, remain ill-defined. Thus, the objectives of this study were to characterize the fecal microbiome, fecal metabolome, and circulating lipidome in obese and non-obese horses. The fecal microbiota, fecal metabolome, and serum lipidome were evaluated in obese (case) horses (n = 20) and non-obese (control) horses (n = 20) matched by farm of origin (n = 7). Significant differences in metabolites of the mitochondrial tricarboxylic acid cycle and circulating free fatty acids were identified in the obese horses compared to the non-obese horses. These results indicate that the host and bacterial metabolism should be considered important in obese horses. Further studies to determine whether these associations are causal and the mechanistic basis of the association are warranted because they might reveal diagnostic biomarkers and therapeutic interventions to mitigate obesity, EMS, and sequelae including laminitis.

CASE SERIES: CLINICAL SALMONELLOSIS IN FOUR BLACK RHINOCEROS (DICEROS BICORNIS) CALVES.

Although Salmonella spp. infection has been identified in captive and free-ranging rhinoceros, clinical cases in black rhinoceros ( Diceros bicornis ) calves have not been described. This case series describes clinical salmonellosis in four black rhinoceros calves. Two calves developed self-limiting diarrhea, recovering after treatment. The other two cases were fatal. One of the fatal cases had a short clinical course, whereas the other case was protracted, with signs reflecting multiple organ system involvement. In all cases, diagnosis was by fecal culture and/or quantitative polymerase chain reaction. A variable clinical presentation, which is typical for salmonellosis in domestic hoofstock, was a feature of these rhinoceros cases. Similarly, postmortem pathology in black rhinoceros calves was consistent with domestic neonatal ungulates with salmonellosis. Potential predisposing factors for infection were considered to be primiparity of the dam and failure of passive transfer in the calf. The case investigation included attempts to identify the source of infection, which was aided by organism serotyping. In one case, the patient's dam and another conspecific in the facility were shown to be asymptomatic shedders of the organism strain responsible for disease in the calf. Further surveillance of captive rhinoceros Salmonella spp. carrier status is needed to inform screening recommendations for this taxa.

Adjuvant immunotherapy of C6 glioma in rats with pertussis toxin.

PURPOSE: In spite of the recent advances in surgery and antitumor drugs, the brain tumors, like glioblastoma, have shown a poor prognosis. The aim of this study was to determine the effect of pertussis toxin (PTx) as immunomodulatory molecule on glial tumors induced by C6 glioma cells. METHODS: Given the pleiotropic effect of PTx on the immune system, we analyzed the effect of PTx on CD4+/CD25+/FoxP3+ (Treg) cells like as immunotherapeutic adjuvant. Thirty rats with a glial tumor of 1.5 cm in diameter were separated in two groups: the first group was treated with PTx and the second group was non-treated (controls). Tumoral volume was measured weekly; tumor, blood and spleen were taken for analysis of subpopulations of T cells, apoptotic index and cytokine contents, in both groups. RESULTS: We observed a significant decrease in tumor volume in the PTx group; this was associated with a decreased in the number of Treg cells, in both spleen and tumor. The percentage of apoptotic cells was increased as compared with that of controls. The production of proinflammatory cytokines was increased in mRNA for IL-6 as well as a small increase in the mRNA expression of perforin and granzime in tumors from rats treated with PTx. No changes were found in the mRNA expression of MCP-1 and MIP-1α. CONCLUSION: These results suggest that PTx could be an immunotherapeutic adjuvant in the integral therapy against glial tumors.

Estenosis aguda de la arteria carótida estudiada con Resonancia Magnética en perros / Acute stenosis of the carotid artery, studied by magnetic resonance in dogs

Se valoró la utilidad de la Angiorresonancia magnética (ARM), para determinar el grado de estenosis producida en nueve perros, a los que se les practicó estenosis de la arteria carótida común mediante plicadura y colocación de injerto de pericardio bovino. Los resultados se compararon entre diferentes modalidades de imagen (ARM, Ultrasonido Doppler, y Angiografía con Subtracción Digital), así como con los hallazgos histopatológicos. Como resultado, se comprobó mediante ARM la permeabilidad de los vasos en siete animales, mientras que por histopatología se demostró que existió permeabilidad en 100 por ciento de los mismos. La ARM es un estudio útil para demostrar el sitio de estenosis y como método de seguimiento no invasivo en cirugía por estenosis carotídea aunque cuando se requiere una valoración cuantitativa es necesario recurrir a otras modalidades de imagen

El terremoto de Fraijanes de 1888 y el sistema de fallas de Alajuela : Implicaciones del peligro sísmico potencial para el Valle Central Occidental

El estudio geológico estructural y morfotectónico de la región al norte de la ciudad de Alajuela, muestra una serie de fallas, estructuras volcánicas y alineamientos que pueden ser correlacionados con el terremoto de 1888, o bien, ser nuevas fuentes sísmicas, siendo la falla de Alajuela la alternativa más viable para el origen del terremoto y una depresión 1.5 Km al este de Fraijanes, la probable área epicentral. Los datos obtenidos de los informes y periódicos de la época permiten estimar el patrón de distribución de las isosistas del terremoto del 30 de diciembre de 1888 y calcular junto con otros datos microsísmicos y a partir de relaciones empíricas, los parámetros sísmicos (profundidad, epicentro, magnitud, intensidad, aceleración). El terremoto de Fraijanes demostró hace casi un siglo ser muy destructivo, y hoy día con el aumento de población e infraestructura en la región, el grado de vulnerabilidad es mayor y por consiguiente aumenta el riesgo sísmico (AU)