Influence of reinforcing materials on strain of maxillary complete denture.

OBJECTIVE: A maxillary complete denture has been a common prosthetic treatment for people with edentulous maxillas. The fracture and deformation of dentures are recurrent and common problems for denture-wearers. Consequently, reinforcement materials are embedded in the denture base to prevent fracture and deformation. The purpose of this study was to examine the influence of reinforcing materials on the strain within the acrylic base of a maxillary complete denture. MATERIALS AND METHODS: Three reinforcements (cast cobalt-chromium bar; glass fibers; and cobalt-chromium wire) were embedded in the bases and a strain-gauge was attached at three positions (labial, middle and posterior) at the mid-line of the polished surface of each denture. A vertical occlusal load of 49 N was applied to the left and right side in the region of the 1st premolar and the 1st molar. Comparisons of the strain were made via ANOVA. RESULTS: The strain of the denture base with a cast cobalt-chromium reinforcement was significantly (p < 0.05) smaller than that with the other reinforcements. CONCLUSIONS: This result suggested that a cast cobalt-chromium reinforcement helps to reduce the risk of fracture and deformation of a maxillary complete denture.

Identification of CD123+ myeloid dendritic cells as an early-stage immature subset with strong tumoristatic potential.

CD123 has been identified as a specific surface marker for plasmacytoid dendritic cells (PDCs). However, CD123 has recently been shown to be expressed on freshly isolated or in vitro generated myeloid dendritic cells (MDCs). In this article, we investigated whether the expression of CD123 on monocyte-derived MDCs was related to their function, especially to tumor-inhibiting potential. MDCs were induced from cord blood CD14+ monocytes with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) for 7 days, and then CD123+ cells were isolated by positive immunomagnetic cell selection. We observed that CD123+ cells lost monocyte CD14 expression, acquired immature myeloid dendritic cell phenotype and morphology. They exerted more significant endocytosis and less antigen-presenting function than CD123(-)MDCs which are often referred to as typical MDCs. Meanwhile, CD123+ MDCs exhibited more significant tumor-inhibiting activity toward hematological tumor cell lines of U937 and Jurkat even at a low effector:target ratio. CD123+ MDCs expressed higher level of cytoplasmic TNF-alpha-related apoptosis-inducing ligand (TRAIL), but no detectable surface TRAIL and very little soluble TRAIL. Pretreatment with recombinant human TRAIL receptor 2:Fc fusion protein significantly reduced the tumor-inhibiting effect of CD123+ MDCs, but not of CD123(-) MDCs. Overall, our data demonstrated that CD123+ MDCs were an early-stage immature DC subset, with a significant tumor-inhibiting activity partially via involvement of enhanced cytoplasmic TRAIL.