Cell and tissue interactions of Treponema pallidum in primary and secondary syphilitic skin lesions: an ultrastructural study of serial sections.

There are limited reports on the ultrastructure of syphilis skin lesions. The aim of this study has been to perform an electron microscopic investigation of the morphology and the tissue distribution of treponemes in primary and secondary cutaneous lesions. Three cases of primary syphilitic chancre and one case of secondary syphilis were included. Prominent epidermal abnormalities in the primary chancre and a perivascular inflammatory infiltrate in the secondary lesion were found by light microscopy. Ultrastructurally, spirochetes were located mainly in the blood vessel walls and dermal tissue of the chancre lesions. In the secondary syphilis case, spirochetes were more abundant between epidermal keratinocytes. Most of them adjusted to the intercellular spaces. Occasionally, the electron microscopy images were highly suggestive of an intracellular location. Both the ultrastructural and immunohistochemical examination of the primary and secondary syphilis lesions showed a paradoxical distribution of the causative microorganisms compared to the light microscopic changes. In addition, the ultrastructural findings strongly suggest that Treponema pallidum subspecies pallidum invades tissues, not only through an intercellular, but also through a transcellular pathway.

CD10 y mesotelioma maligno. A propósito de un caso / CD10 and malignant mesothelioma. Report of a case

Aportamos un caso de mesotelioma maligno en unamujer de 37 años con antecedentes de laparoscopia por leiomiomasuterinos. El diagnostico de mesotelioma se estableciópor autopsia. El estudio inmunohistoquímico del tumormesotelial, reveló intensa positividad para CD10, y ausenciade inmunoreactividad para receptores de estrógenos y deprogesterona y calretinina. En nuestra serie de mesoteliomasobservamos que alrededor del 30% de ellos expresabanCD10. En la revisión de la Literatura se observó que aproximadamenteel 40% de los mesoteliomas, mostraban inmunoreactividadpara CD10. En conslusión nuestras observacionesmuestran que CD10 puede expresarse de formaextensa en los mesoteliomas, dato que puede ayudar al establecerun diagnóstico diferencial en estos casos

We report a case of malignant mesothelioma in a 37year-old woman treated for uterine leiomyomas. The diagnosisof mesothelioma was stablished based on autopsystudy. The immunohistochemical study of mesothelialtumor showed strong CD10 immunoreactivity. Our series ofmesotheliomas was also retrospectively studied with anti-CD10 and around 30% of them expressed CD10. Around40% of mestohelioma cases reported in the Literatureexpress CD10. We conclude that CD10 could be expressedin some mesothelial tumors, and may help to stablish a differentialdiagnosis in some cases