Comparative Growth Inhibition of Bread Spoilage Fungi by Different Preservative Concentrations Using a Rapid Turbidimetric Assay System.

Bread and intermediate moisture bakery products are mainly spoiled by yeasts and filamentous fungi. The inoculum load and preservation system used determines their shelf life. To extend the shelf life of such commodities, the use of chemical preservatives is the most common way to try and control the initiation of mold spoilage of bread. This study has utilized a rapid turbidimetric assay system (Bioscreen C) to examine the temporal efficacy of calcium propionate (CP) and potassium sorbate (PS) for controlling the growth of important bread spoilage fungi. The objectives were to compare the temporal growth of strains of three important spoilage fungi Hyphopichia burtonii (HB17), Paecilomyces variotii (PV11), and Penicillium roqueforti (PR06) isolated from visibly molded bread to (a) different concentrations of CP and PS (0-128 mM), (b) temperatures (25°C, 30°C), (c) water activity (aw; 0.95, 0.97), and (d) pH (5.0, 5.5). All three abiotic factors, pH, aw, and temperature, and preservative concentrations influenced the relative growth of the species examined. In general, PS was more effective than CP in inhibiting the growth of the strains of these three species. In addition, the Time to Detection (TTD) for the efficacy of the preservatives under the interacting abiotic factors was compared. The strain of Paecilomyces variotii (PV10) was the most tolerant to the preservatives, with the shortest TTD values for both preservatives. P. roqueforti was the most sensitive with the longest TTD values under all conditions examined. These results are discussed in the context of the evolution of resistance to food-grade preservatives by such spoilage fungi in bakery products.

Overview of Fungi and Mycotoxin Contamination in Capsicum Pepper and in Its Derivatives.

Capsicum products are widely commercialised and consumed worldwide. These substrates present unusual nutritional characteristics for microbial growth. Despite this, the presence of spoilage fungi and the co-occurrence of mycotoxins in the pepper production chain have been commonly detected. The main aim of this work was to review the critical control points, with a focus on mycotoxin contamination, during the production, storage and distribution of Capsicum products from a safety perspective; outlining the important role of ecophysiological factors in stimulating or inhibiting mycotoxin biosynthesis in these food commodities. Moreover, the human health risks caused by the ingestion of peppers contaminated with mycotoxins were also reviewed. Overall, Capsicum and its derivative-products are highly susceptible to contamination by mycotoxins. Pepper crop production and further transportation, processing and storage are crucial for production of safe food.

Temperature and water activity effects on growth and temporal deoxynivalenol production by two Argentinean strains of Fusarium graminearum on irradiated wheat grain.

The objective of this study was to determine the effects of water activity (a(W); 0.900-0.995), temperature (5, 15, 25 and 30 degrees C), time of incubation (7-49 days) and their interactions on mycelial growth and deoxynivalenol (DON) production on irradiated wheat grain by two strains of Fusarium graminearum isolated from wheat ears in Argentina. Optimal a(W) levels for growth were in the range 0.950-0.995 with a temperature optima of 25 degrees C. Maximum growth rates were obtained at the highest a(W) (0.995) and 25 degrees C for both strains. No growth was observed at 5 degrees C regardless of the a(W) levels assayed. Both strains were able to growth at the lowest a(W) assayed (0.900), although the temperature ranges allowing growth at this minimal a(W) was 15-25 degrees C. DON was produced the most rapidly (7 days) when incubated at 25 degrees C and 0.995 a(W). All other conditions required 7-14 days before DON was produced on grain. Maximum amounts of DON for both strains were produced at the highest a(W) treatment (0.995) after 6 weeks at 30 degrees C. The range of DON concentrations varied considerably (5 to 140,000 ng g(-1)) depending on a(W) and temperature interaction treatments. Production of DON occurred over a narrower range of a(W) (0.995-0.95) than that for growth (0.995-0.90). DON was more rapidly produced at 25 degrees C but the maximum amount produced was at 30 degrees C. Two-dimensional profiles of a(W) x temperature were developed from these data to identify areas where conditions indicate a significant risk from DON accumulation.