Accelerating the humification of mushroom waste by regulating nitrogen sources composition: Deciphering mechanism from bioavailability and molecular perspective.

Regulating nitrogen source composition is efficient approach to accelerate the spent mushroom substrate (SMS) composting process. However, currently, most traditional composting study only focuses on total C/N ratio of initial composting material. Rarely research concerns the effect of carbon or nitrogen components at different degradable level and their corresponding decomposed-substances on humification process. This study deciphers and compares the mechanism of mixed manure-N sources on SMS humification from bioavailability and molecular perspective. Two different biodegradable manure-N sources, cattle manure (CM) and Hainan chicken manure (CH), were added into the SMS composting with the different CM:CH ratio of 1:0, 3:1, 1:1, 1:3, and 0:1, respectively. The physicochemical properties and humic substances were determined to evaluate the compost quality. Coupling analysis of spectroscopy, fluorescence, and humic intermediate precursors were conducted to characterizing molecular formation process of humic acid (HA). The results indicated that regulating the carbon-nitrogen nutrient biodegradability of composting material by adding mixed nitrogen sources is an effective strategy to accelerate the SMS humification process. The C1H3 (CM:CH ratio of 1:3) and CH treatments obtained great physicochemical properties and the highest growth rate of HA (31.96% and 27.02%, respectively). The rapid reaction of polysaccharide, ketone, quinone, and amide in DOM (LCP1) might be the key for the fast humification in C1H3 and CH. The polyphenol, reducing sugar and amino acid originated from the labile-carbon-proportion I (LCP1) and recalcitrant-carbon-proportion (RCP), labile-carbon-proportion II (LCP2) and RCP, and labile-nitrogen-proportion I (LNP1), respectively, were the main driving intermediate precursors for the formation of HA. This study deciphers the SMS humification mechanism at molecular level and provides a strategy in accelerating-regulating the composting process. which will be beneficial for enhancing the disposing efficiency of SMS, producing high-quality organic fertilizer, and even popularizing to the similar types of organic waste in practical field.

Insight into humification of mushroom residues under addition of Rich-N sources: Comparing key molecular evolution processes using EEM-PARAFAC and 2D-FTIR-COS analysis.

Accelerating the humification of organic solid waste is one of the most important issues in composting. This present study aims to study and compare the humification process of different rich-N sources (chicken manure, cattle manure, and urea) addition during the composting of mushroom residues, from macro physicochemical properties to micro humic molecular structure evolution process. The physicochemical elements and humic components were determined for evaluating the compost quality and humification degree as composting proceed. The coupled analysis of excitation-emission matrix with parallel factor analysis (EEM-PARAFAC) and two-dimensional correlation with Fourier transform infrared spectrum (2D-FTIR-COS) were used to characterize the functional molecular structure evolution of dissolved organic matter during humification process. The results indicated that the rank order for humification level were the treatments of chicken manure (HM), urea (UM), cattle manure (CM), and single mushroom residue treatment (CK), with their humification index of 22.18%, 22.05%, 18.47%, and 16.52%, respectively. Humic substance, humic acid, and fulvic acid were obtained the highest in HM treatment with contents of 35.41 ± 0.86%, 23.32 ± 1.57%, and 10.97 ± 0.52%, respectively. The rich-N source addition enhanced the degradation of protein-like and polysaccharides-like substances in dissolved organic matter, thus accelerating the humification process of mushroom residues. The key structure evolution of dissolved organic matter in the HM treatment, in which the CO and CC stretching of quinone, amide, or ketone, and the C-O stretching of polysaccharides may be responsible for the faster formation of humus compared to the other nitrogen treatments. In this study, redundancy analysis indicated that the total nitrogen (TN) and nitrate nitrogen (NO3--N) may be the potential indicators for determining the humification level as composting proceed. The result provides significant insight into the humification mechanism of mushroom residue under different types of nitrogen sources at the molecular level, and will be reference for improving the composting technique in practical field.

Regulation of retinoic acid synthetic enzymes by WT1 and HDAC inhibitors in 293 cells.

All-trans retinoic acid (atRA), which is mainly generated endogenously via two steps of oxidation from vitamin A (retinol), plays an indispensible role in the development of the kidney and many other organs. Enzymes that catalyze the oxidation of retinol to generate atRA, including aldehyde dehydrogenase 1 family (ALDH1)A1, ALDH1A2 and ALDH1A3, exhibit complex expression patterns at different stages of renal development. However, molecular triggers that control these differential expression levels are poorly understood. In this study, we provide in vitro evidence to demonstrate that Wilms' tumor 1 (WT1) negatively regulates the expression of the atRA synthetic enzymes, ALDH1A1, ALDH1A2 and ALDH1A3, in the 293 cell line, leading to significant blockage of atRA production. Furthermore, we demonstrate that the suppression of ALDH1A1 by WT1 can be markedly attenuated by histone deacetylase inhibitors (HDACis). Taken together, we provide evidence to indicate that WT1 and HDACs are strong regulators of endogenous retinoic acid synthetic enzymes in 293 cells, indicating that they may be involved in the regulation of atRA synthesis.

Cordycepin induces apoptosis and autophagy in human neuroblastoma SK-N-SH and BE(2)-M17 cells.

Cordycepin, also termed 3'-deoxyadenosine, is a derivative of the nucleoside adenosine that represents a potential novel class of anticancer drugs targeting the 3' untranslated region of RNAs. Cordycepin has been reported to induce apoptosis in certain cancer cell lines, but the effects of cordycepin on human neuroblastoma cells have not been studied. In the present study, an MTT assay revealed that cordycepin inhibits the viability of neuroblastoma SK-N-SH and BE(2)-M17 cells in a dose-dependent manner. In addition, cordycepin increases the early-apoptotic cell population of SK-N-SH cells, as determined by fluorescence-activated cell sorting analysis. The induction of apoptosis in neuroblastoma cells by cordycepin was further confirmed by western blotting, which revealed cleavage of caspase-3 and poly(adenosine diphosphate-ribose) polymerase 1 in the SK-N-SH and BE(2)-M17 cells. Cordycepin also induced the formation of a punctate pattern of light-chain 3 (LC3)-associated green fluorescence in the SK-N-SH cells transfected with a pEGFP-LC3 vector. Furthermore, western blotting revealed cleavage of LC3 A/B in cordycepin-treated neuroblastoma SK-N-SH cells. Taken together, the results indicate that cordycepin significantly increases apoptosis and autophagy in neuroblastoma cells, and may therefore be a drug candidate for neuroblastoma therapy, but requires additional evaluation.