A screening method for heavy metals in consumer goods using reactive desorption electrospray ionization mass spectrometry.

RATIONALE: Contamination of everyday goods with heavy metals such as nickel, cadmium, and lead known to be hazardous to the health of customers is an ongoing problem. METHOD: Here, a mass spectrometric screening method based on reactive desorption electrospray ionization (DESI) is presented for the analysis of metals in consumer goods such as jewelry, tableware, and paintings. The method detects oxidized species of lead, nickel, cadmium, copper, and iron from the surface of objects without sample preparation. Positively charged metal ions form singly and doubly negatively charged complexes with ethylenediaminetetraacetic acid added to the DESI solvent, which are analyzed by a mass spectrometer. RESULTS: Qualitative and quantitative performance of the method was elucidated with metal salt standards. Subsequently, authentic samples were analyzed qualitatively. Reactive DESI-MS was able to detect lead and cadmium in eight out of nine consumer goods. For tableware, these heavy metals were found to be localized in the print as determined by reactive DESI imaging. In addition, mockup paintings generated from modern and historical pigments of Pb, Cu, Cd, and Fe in various media (acrylic binder, egg tempera, and linseed oil) were measured to show the suitability of the method for art authentication and conservation. CONCLUSION: The developed method expands the range of analytes accessible by DESI-MS to metal ions. Hence, DESI becomes a suitable ionization technique for an increasing number of analyte classes, which are of interest in chemical screening of consumer goods.

Antiviral Potential of Natural Resources against Influenza Virus Infections.

Influenza is a severe contagious disease caused by influenza A and B viruses. The WHO estimates that annual outbreaks lead to 3-5 million severe infections of which approximately 10% lead to the death of the patient. While vaccination is the cornerstone of prevention, antiviral drugs represent the most important treatment option of acute infections. Only two classes of drugs are currently approved for the treatment of influenza in numerous countries: M2 channel blockers and neuraminidase inhibitors. In some countries, additional compounds such as the recently developed cap-dependent endonuclease inhibitor baloxavir marboxil or the polymerase inhibitor favipiravir are available. However, many of these compounds suffer from poor efficacy, if not applied early after infection. Furthermore, many influenza strains have developed resistances and lost susceptibility to these compounds. As a result, there is an urgent need to develop new anti-influenza drugs against a broad spectrum of subtypes. Natural products have made an important contribution to the development of new lead structures, particularly in the field of infectious diseases. Therefore, this article aims to review the research on the identification of novel lead structures isolated from natural resources suitable to treat influenza infections.

Antimicrobial, Insecticidal and Cytotoxic Activity of Linear Venom Peptides from the Pseudoscorpion Chelifer cancroides.

Linear cationic venom peptides are antimicrobial peptides (AMPs) that exert their effects by damaging cell membranes. These peptides can be highly specific, and for some, a significant therapeutic value was proposed, in particular for treatment of bacterial infections. A prolific source of novel AMPs are arthropod venoms, especially those of hitherto neglected groups such as pseudoscorpions. In this study, we describe for the first time pharmacological effects of AMPs discovered in pseudoscorpion venom. We examined the antimicrobial, cytotoxic, and insecticidal activity of full-length Checacin1, a major component of the Chelifer cancroides venom, and three truncated forms of this peptide. The antimicrobial tests revealed a potent inhibitory activity of Checacin1 against several bacteria and fungi, including methicillin resistant Staphylococcus aureus (MRSA) and even Gram-negative pathogens. All peptides reduced survival rates of aphids, with Checacin1 and the C-terminally truncated Checacin11-21 exhibiting effects comparable to Spinosad, a commercially used pesticide. Cytotoxic effects on mammalian cells were observed mainly for the full-length Checacin1. All tested peptides might be potential candidates for developing lead structures for aphid pest treatment. However, as these peptides were not yet tested on other insects, aphid specificity has not been proven. The N- and C-terminal fragments of Checacin1 are less potent against aphids but exhibit no cytotoxicity on mammalian cells at the tested concentration of 100 µM.