RESUMO
Inflammatory bowel disease (IBD) may result from exaggerated stimulation of the mucosal immune system by luminal bacterial flora. Bacterial products are recognized by pattern recognition receptors such as Toll-like receptors (TLRs), which are key regulators of the innate immune system. Therefore, the expression of TLR2, TLR3 and TLR4 in colonic biopsy samples taken from children with active IBD were studied and compared to controls. Colonic biopsy samples were collected from macroscopically inflamed and non-inflamed regions of the mucosa of 12 children with freshly diagnosed IBD (fdIBD) and 23 children with relapsed IBD (rIBD). Specimens were also obtained from eight controls. TLR2, TLR3 and TLR4 mRNA expression and protein levels were determined by real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blot. We found higher TLR2 and TLR4 mRNA and protein levels in the inflamed colonic mucosa of children with fdIBD and rIBD compared to controls. In the non-inflamed colonic mucosa of children with fdIBD and rIBD, TLR2 and TLR4 mRNA and protein levels were similar to controls. TLR2 and TLR4 mRNA and protein levels also did not differ between children with fdIBD or rIBD in either inflamed or non-inflamed colonic mucosa. TLR3 mRNA expression and protein levels were similar in all groups studied. Our results of increased levels of TLR2 and TLR4 in the inflamed colonic mucosa of children with IBD confirm the hypothesis that innate immunity has an important role in the pathogenesis of this disease.
Assuntos
Colo , Doenças Inflamatórias Intestinais/metabolismo , Mucosa Intestinal/metabolismo , Receptor 2 Toll-Like/metabolismo , Receptor 4 Toll-Like/metabolismo , Adolescente , Western Blotting/métodos , Estudos de Casos e Controles , Criança , Suscetibilidade a Doenças , Feminino , Humanos , Imunidade Inata , Doenças Inflamatórias Intestinais/imunologia , Mucosa Intestinal/imunologia , Masculino , RNA Mensageiro/análise , Recidiva , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Estatísticas não Paramétricas , Receptor 2 Toll-Like/análise , Receptor 2 Toll-Like/genética , Receptor 3 Toll-Like/análise , Receptor 3 Toll-Like/genética , Receptor 3 Toll-Like/metabolismo , Receptor 4 Toll-Like/análise , Receptor 4 Toll-Like/genéticaRESUMO
The authors describe a 14-year-old gypsy girl with the clinical picture of exudative enteropathy where they observed on X-ray examination of the abdominal cavity circular formations which changed position. On laparotomy they found on the serous membrane of the entire enlarged and inflamed ileum numerous pseudo-polypous formations the majority of which were released and floated freely in the chylous ascites. The description of similar globular lesions of the lymphatic system was found in the literature only in accounts on post-mortem findings.
