Gastrin induction of protein synthesis in isolated gastric mucosal cells.

Exposure of isolated rat gastric mucosal cells to 10(-10) and 10(-9) M gastrin (G-17-I) for 2 hr significantly stimulated [3H]leucine incorporation (15 min pulse) into protein by 100 and 212%, respectively, when compared with the basal levels. Doses beyond 10(-9) M lowered the maximal stimulatory effect of the hormone. Gastrin (10(-9) M) specifically stimulated the synthesis of five proteins in isolated gastric mucosal cells with apparent molecular weights of 105, 76, 71, 63, and 54 kDa. Actinomycin-D (10 micrograms/ml) completely abolished the gastrin-mediated stimulation of protein synthesis in isolated gastric mucosal cells.