Hexagonal Boron Nitride Quantum Dots Embedded on Layer-by-Layer Films for Peroxidase-Assisted Colorimetric Detection of ß-Galactosidase Producing Pathogens.

Pathogen detection has become a major research area all over the world for water quality surveillance and microbial risk assessment. Therefore, designing simple and sensitive detection kits plays a key role in envisaging and evaluating the risk of disease outbreaks and providing quality healthcare settings. Herein, we have designed a facile and low-cost colorimetric sensing strategy for the selective and sensitive determination of ß-galactosidase producing pathogens. The hexagonal boron nitride quantum dots (h-BN QDs) were established as a nanozyme that showed prominent peroxidase-like activity, which catalyzes 3,3',5,5'-tetramethylbenzidine (TMB) oxidation by H2O2. The h-BN QDs were embedded on a layer-by-layer assembled agarose biopolymer. The ß-galactosidase enzyme partially degrades ß-1,4 glycosidic bonds of agarose polymer, resulting in accessibility of h-BN QDs on the solid surface. This assay can be conveniently conducted and analyzed by monitoring the blue color formation due to TMB oxidation within 30 min. The nanocomposite was stable for more than 90 days and was showing TMB oxidation after incubating it with Escherichia coli (E. coli). The limit of detection was calculated to be 1.8 × 106 and 1.5 × 106 CFU/mL for E. coli and Klebsiella pneumonia (K. pneumonia), respectively. Furthermore, this novel sensing approach is an attractive platform that was successfully applied to detect E. coli in spiked water samples and other food products with good accuracy, indicating its practical applicability for the detection of pathogens in real samples.

DNA Carbon-Nanodots based Electrochemical Biosensor for Detection of Mutagenic Nitrosamines.

Mutagenic and Carcinogenic substances are a threat to any living organism, and its detection is of paramount importance. In this work, we fabricate for the first time a DNA-carbon dots based electrochemical biosensor for sensitive and selective detection of mutagenic nitrosamines like N-nitrosodimethylamine (NDMA) and N-nitrosodiethanolamine (NDEA). At first, on the glassy carbon electrode (GCE), chitosan carbon dot was deposited, then, DNA was electro-statically immobilizing on the surface of carbon dots to fabricate the sensing electrode (DNA/chiCD/GCE modified electrode). In the presence of NDMA and NDEA, in differential pulse voltammetry technique, the absolute peak current increases, and thus it can detect NDMA and NDEA. The system DNA/chiCD/GCE modified electrode is highly selective and sensitive toward NDMA and NDEA. The detection limit was determined to be 9.9 × 10-9 M and 9.6 × 10-9 M, respectively. The possible reason for DNA/chiCD/GCE modified electrode showing such electrochemical selectivity toward nitrosamines is investigated and discussed.

Carbon-Dot-Coated Alginate Beads as a Smart Stimuli-Responsive Drug Delivery System.

In this work, we report a smart stimuli-responsive drug delivery system (DDS) that can release drug depending upon the amount of pathogen (MRSA) present in the target. A greater amount of MRSA in the system will lead to more release of drug and vice versa. Carbon-dot-coated novel alginate beads (CA-CD) exhibiting superior stability was successfully used as smart drug delivery vehicle. Garlic extract (GE), which contains allicin, was taken as model drug system to demonstrate the phenomena. It was observed that GE loading was 19 and 78% with CA and CA-CD, respectively. CA-CD-GE shows pH-dependent controlled drug release, which results in increased therapeutic efficiency. CA-CD-GE is not only stimuli responsive but also a controlled drug release system as it releases drug according to the pathogen concentration (MRSA). All the three factors viz. drug release, MRSA concentration and pH of the medium are interdependent as when the cell divides, it produces secondary metabolites that lead to the decrease in pH of the medium. The drop in the pH value triggers drug release from the beads. And the effect of the drug is reflected by the MRSA cell death. Hence, we demonstrate a smart stimuli responsive DDS. However, such DDS will be useful in cases where increased amount of pathogen in the system will lead to reduction in pH.