A registered report testing the effect of sleep on Deese-Roediger-McDermott false memory: greater lure and veridical recall but fewer intrusions after sleep.

Human memory is known to be supported by sleep. However, less is known about the effect of sleep on false memory, where people incorrectly remember events that never occurred. In the laboratory, false memories are often induced via the Deese-Roediger-McDermott (DRM) paradigm where participants are presented with wordlists comprising semantically related words such as nurse, hospital and sick (studied words). Subsequently, participants are likely to falsely remember that a related lure word such as doctor was presented. Multiple studies have examined whether these false memories are influenced by sleep, with contradictory results. A recent meta-analysis suggests that sleep may increase DRM false memory when short lists are used. We tested this in a registered report (N = 488) with a 2 (Interval: Immediate versus 12 h delay) × 2 (Test Time: 9:00 versus 21:00) between-participant DRM experiment, using short DRM lists (N = 8 words/list) and free recall as the memory test. We found an unexpected time-of-day effect such that completing free recall in the evening led to more intrusions (neither studied nor lure words). Above and beyond this time-of-day effect, the Sleep participants produced fewer intrusions than their Wake counterparts. When this was statistically controlled for, the Sleep participants falsely produced more critical lures. They also correctly recalled more studied words (regardless of intrusions). Exploratory analysis showed that these findings cannot be attributed to differences in output bias, as indexed by the number of total responses. Our overall results cannot be fully captured by existing sleep-specific theories of false memory, but help to define the role of sleep in two more general theories (Fuzzy-Trace and Activation/Monitoring theories) and suggest that sleep may benefit gist abstraction/spreading activation on one hand and memory suppression/source monitoring on the other.

Episodic memory and sleep are involved in the maintenance of context-specific lexical information.

Familiar words come with a wealth of associated knowledge about their variety of usage, accumulated over a lifetime. How do we track and adjust this knowledge as new instances of a word are encountered? A recent study (Cognition) found that, for homonyms (e.g., bank), sleep-associated consolidation facilitates the updating of meaning dominance. Here, we tested the generality of this finding by exposing participants to (Experiment 1; N = 125) nonhomonyms (e.g., bathtub) in sentences that biased their meanings toward a specific interpretation (e.g., bathtub-slip vs. bathtub-relax), and (Experiment 2; N = 128) word-class ambiguous words (e.g., loan) in sentences where the words were used in their dispreferred word class (e.g., "He will loan me money"). Both experiments showed that such sentential experience influenced later interpretation and usage of the words more after a night's sleep than a day awake. We interpret these results as evidence for a general role of episodic memory in language comprehension such that new episodic memories are formed every time a sentence is comprehended, and these memories contribute to lexical processing next time the word is encountered, as well as potentially to the fine-tuning of long-term lexical knowledge. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Word-meaning priming extends beyond homonyms.

When a homonym (e.g., bark) is encountered in a sentential context that biases its interpretation towards a less frequent meaning, subsequent interpretations of the word are more likely to favour that subordinate meaning. Such word-meaning priming effects have been shown to be maintained via sleep-related consolidation, leading some to suggest that declarative memory systems play a crucial role in language comprehension, providing a relatively enduring contextually bound memory trace for the ambiguous word. By this account, word-meaning priming effects should be observable for all words, not just homonyms. In three experiments, participants were exposed to non-homonym targets (e.g., "balloon") in sentences that biased interpretation towards a specific aspect of the word's meaning (e.g., balloon­helium vs. balloon-float). After a ~ 10-30 min delay, the targets were presented in relatedness judgement and associate production tasks to assess whether the sentential contexts enhanced access to the primed aspect of the word's meaning. The results reveal that word-meaning priming effects do extend to non-homonyms. Indeed, there was also some evidence of a more generalised priming that did not rely on prior presentation of the non-homonym itself. We argue that context-specific interpretations of words are maintained during recognition in order to facilitate comprehension over longer periods.

Lexical connectivity effects in immediate serial recall of words.

In six experiments, we tested whether immediate serial recall is influenced by a word's degree centrality, an index of lexical connectivity. Words of high degree centrality are associated with more words in free association norms than those of low degree centrality. Experiment 1 analyzed secondary data to explore the effect of degree centrality in wordlists containing a mixture of high- and low-degree words. High-degree words were advantaged across all serial positions, independently of other variables including word frequency. Experiment 2 replicated this finding using an expanded stimulus set. Experiment 3 used pure lists with each list containing high- or low-degree words only (e.g., HHHHHH vs. LLLLLL). Once again, high-degree words were better recalled across all serial positions. In Experiment 4, each wordlist alternated between high and low-degree words (e.g., HLHLHL and LHLHLH). Recall of low-degree words was facilitated by the neighboring high-degree words, abolishing the overall high-degree advantage. Experiment 5 used a within-participant design and replicated the findings from Experiments 3 and 4 such that the high-degree advantage in pure lists disappeared in alternating lists. Experiment 6 compared high and low frequency words in pure lists while controlling for degree centrality between the item sets. A high-frequency advantage emerged, suggesting that the effects of frequency and degree centrality are separable. We conclude that degree centrality is a distinct psycholinguistic variable that affects serial recall as both (a) an item-level characteristic such that high (vs. low) degree words have greater accessibility in the lexicon and (b) an interitem property such that high-degree words facilitate the recall of neighboring words by enhancing the formation of associative links. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

Evidence for preferential attachment: Words that are more well connected in semantic networks are better at acquiring new links in paired-associate learning.

Here, we view the mental lexicon as a semantic network where words are connected if they are semantically related. Steyvers and Tenenbaum (Cognitive Science, 29, 41-78, 2005) proposed that the growth of semantic networks follows preferential attachment, the observation that new nodes are more likely to connect to preexisting nodes that are more well connected (i.e., the rich get richer). If this is the case, well-connected known words should be better at acquiring new links than poorly connected words. We tested this prediction in three paired-associate learning (PAL) experiments in which participants memorized arbitrary cue-response word pairs. We manipulated the semantic connectivity of the cue words, indexed by the words' free associative degree centrality. Experiment 1 is a reanalysis of the PAL data from Qiu and Johns (Psychonomic Bulletin & Review, 27, 114-121, 2020), in which young adults remembered 40 cue-response word pairs (e.g., nature-chain) and completed a cued recall task. Experiment 2 is a preregistered replication of Qiu and Johns. Experiment 3 addressed some limitations in Qiu and Johns's design by using pseudowords as the response items (e.g., boot-arruity). The three experiments converged to show that cue words of higher degree centrality facilitated the recall/recognition of the response items, providing support for the notion that better-connected words have a greater ability to acquire new links (i.e., the rich do get richer). Importantly, while degree centrality consistently accounted for significant portions of variance in PAL accuracy, other psycholinguistic variables (e.g., concreteness, contextual diversity) did not, suggesting that degree centrality is a distinct variable that affects the ease of verbal associative learning.

Why and how the co-occurring familiar object matters in Fast Mapping (FM)? Insights from computational models.

This article uses insights from computational semantic networks to explain why the co-occurring familiar objects are critical to the Fast Mapping (FM) procedure. I first propose that the co-occurring familiar objects provide the novel targets with a 'mimicry opportunity', which may facilitate the establishment of targets in long-term cortical memory networks. I then argue that the occurrence of rapid cortical learning may depend on how 'well-connected' the co-occurring familiar object is in long-term memory networks.

The Evolving Science of Trauma Resuscitation.

This review summarizes the evolution of trauma resuscitation from a one-size-fits-all approach to one tailored to patient physiology. The most dramatic change is in the management of actively bleeding patients, with a balanced blood product-based resuscitation approach (avoiding crystalloids) and surgery focused on hemorrhage control, not definitive care. When hemostasis has been achieved, definitive resuscitation to restore organ perfusion is initiated. This approach is associated with decreased mortality, reduced duration of stay, improved coagulation profile, and reduced crystalloid/vasopressor use. This article focuses on the tools and methods used for trauma resuscitation in the acute phase of trauma care.

Hepatitis E virus: seroprevalence and frequency of viral RNA detection among US blood donors.

BACKGROUND: Hepatitis E virus (HEV) is a nonenveloped emerging virus of increasing worldwide interest. Antibody prevalence, RNA frequencies, and transfusion transmissions have been reported. We investigated the HEV RNA and antibody frequencies in US blood donors. STUDY DESIGN AND METHODS: Individual-donation HEV RNA testing was performed on 18,829 donations from six US geographic regions using a CE-marked nucleic acid test (95% limit of detection, 7.9 IU/mL). Repeat-reactive donations were confirmed by in-house, real-time polymerase chain reaction (PCR; 10.3 IU/mL). Total HEV seroprevalence in a randomly selected subset of donations (n = 4499) was assessed by a direct, double-antigen sandwich assay; reactives were further tested for immunoglobulin (Ig)G and IgM. As part of the total antibody confirmatory algorithm, the cutoff was adjusted. RESULTS: Two donations tested confirmed-positive for RNA (PCR not quantifiable, IgM/IgG positive; and 14 IU/mL, antibody negative) for a frequency of 1 in 9500 (95% confidence interval [CI], 1:2850-1:56,180) and 99.96% specificity (95% CI, 99.92%-99.98%); both donors were from the Midwest United States. Antibody prevalence was 9.5% (95% CI, 8.7-10.5) before the cutoff adjustment and 7.7% (95% CI, 7.0%-8.5%) after adjustment; 0.58% (95% CI, 0.39%-0.85%) were IgM positive. CONCLUSIONS: We confirmed comparatively low rates and low viral loads of HEV RNA in US blood donors indicating the need for individual-donation testing if screening is implemented. Antibody prevalence rates were comparable to those reported by one US study using a different assay, but lower than those reported in another study using yet a third assay. We did not answer the question of whether US blood donation screening is warranted. Selective strategies involving providing HEV-negative blood to severely immunosuppressed patients at risk of developing hepatitis may be considered.

Detection of Avian bornavirus in multiple tissues of infected psittacine birds using real-time reverse transcription polymerase chain reaction.

Avian bornavirus (ABV), the cause of proventricular dilation disease in psittacine birds, has been detected in multiple tissues of infected birds using immunohistochemical staining (IHC) and reverse transcription polymerase chain reaction (RT-PCR). In the current study, real-time RT-PCR, using primers targeting the ABV matrix gene, was used to detect ABV in 146 tissues from 7 ABV-infected psittacine birds. Eighty-six percent of the samples tested positive, with crossing point values ranging from 13.82 to 37.82 and a mean of 22.3. These results were compared to the findings of a previous study using gel-based RT-PCR and IHC on the same samples. The agreement between the 2 RT-PCR techniques was 91%; when tests disagreed it was because samples were negative using gel-based RT-PCR but positive on real-time RT-PCR. Agreement with IHC was 77%; 16 out of 74 samples were negative using IHC but positive on real-time RT-PCR. The results suggest that real-time RT-PCR is a more sensitive technique than gel-based RT-PCR and IHC to detect ABV in tissues. The tissues that were ranked most frequently as having a high amount of viral RNA were proventriculus, kidney, colon, cerebrum, and cerebellum. Skeletal muscle, on the other hand, was found to have a consistently low amount of viral RNA.

Socio-economic disparities in mortality due to pandemic influenza in England.

OBJECTIVES: This study examines variations in mortality between socio-economic groups due to the pandemic Influenza (H1N1) 2009 virus in England. METHODS: We established a system to identify all deaths related to pandemic (H1N1) 2009 influenza. We collected the postcode of every individual who died, and through this determined the socio-economic deprivation, urban-rural characteristics and region of their residence. Across England, we were therefore able to examine how mortality rates varied by socio-economic group, between urban and rural areas, and between regions. RESULTS: People in the most deprived quintile of England's population had an age and sex-standardised mortality rate three times that experienced by the least deprived quintile (RR = 3.1, 95% CI 2.2-4.4). Mortality was also higher in urban areas than in rural areas (RR = 1.7, 95% CI 1.2-2.3). Mortality rates were similar between regions of the country. CONCLUSION: Tackling socio-economic health inequalities is a central concept within public health, but has not always been a part of emergency preparedness plans. These data demonstrate the opportunity to reduce the overall impact and narrow inequalities by considering socio-economic disparities in future pandemic planning.

Engaging trainees in shaping the future of health policy.

This paper presents an analysis of the views and ideas generated at a recent health policy discussion for doctors in training. This provides an illustration of the creativity and enthusiasm that trainees can bring to the policy sphere by providing unique insights and a fresh perspective.

Microfluidic devices harboring unsealed reactors for real-time isothermal helicase-dependent amplification.

High-throughput microchip devices used for nucleic-acid amplification require sealed reactors. This is to prevent evaporative loss of the amplification mixture and cross-contamination, which may occur among fluidically connected reactors. In most high-throughput nucleic-acid amplification devices, reactor sealing is achieved by microvalves. Additionally, these devices require micropumps to distribute amplification mixture into an array of reactors, thereby increasing the device cost, and adding complexity to the chip fabrication and operation processes. To overcome these limitations, we report microfluidic devices harboring open (unsealed) reactors in conjunction with a single-step capillary based flow scheme for sequential distribution of amplification mixture into an array of reactors. Concern about evaporative loss in unsealed reactors have been addressed by optimized reactor design, smooth internal reactor surfaces, and incorporation of a localized heating scheme for the reactors, in which isothermal, real-time helicase-dependent amplification (HDA) was performed.